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The map label for this gene is azoR

Identifier: 170750858

GI number: 170750858

Start: 4747167

End: 4747781

Strand: Direct

Name: azoR

Synonym: Mrad2831_4469

Alternate gene names: 170750858

Gene position: 4747167-4747781 (Clockwise)

Preceding gene: 170750854

Following gene: 170750860

Centisome position: 78.11

GC content: 73.01

Gene sequence:

>615_bases
ATGAAACTCCTCCATCTCGACGCCAGCATCCTGGGCACCGGCTCGGTCTCGCGGGAGCTGTCCGCGCTGATCGTCCGGCG
CCTCGCCGGGGATGCGCCGGACGCCGTGACCTACCGGGATCTCGTCGCCGAGAATCCGCCGCACCTGACGGTCGCGACCC
TGCCGGGTGCCCACCCGGTCTCGGCCATGGCCGGCCCCCTCGACGCCGCCGGACAGGCCGTCCGGGACGCCAGCGACCGG
ATGCTGTCGGAATTCGTCGCCGCCGACACGGTCGTCATCGGCGTGCCGATGTACAATTTCACGATCCCGTCGCAGCTCAA
GGCCTGGATCGACCGCCTGCTCGTGCCGGGCACGACCTTCCGCTACGGCGCCGCCGGGCCGGAGGGGCTGATGGGCGGCA
AGCGCGTGATCCTGGCTCTCGCCCGCGGCGGCTTCTACGGGCCGGGCACGGCCTCCGTCCCGGCGGAGCACGCCGAGCAC
TACCTGCGCACGGTGTTCGGCTTCATGGGCATCGTGCCCGAGCTGGTGCTGGCCGAGGGGCTGGCGGCCGGCGAGCACAA
CAAGGCGCAGGCCCTGGCCTCGGCGCGCGACGCGGTCGGGCAGCTCGCCGCCTGA

Upstream 100 bases:

>100_bases
AGTCACATCCATGTAACCTCGGTATCAACCCTGTGCGTTCTTGCGCGGTCGACGCGCGGCCGTATCCTGATCCGGTCACA
AAGAGGAACCGGGTTACGCT

Downstream 100 bases:

>100_bases
GGCCGAGCGACCGGCCGCGGGGCGGCCGGCGGAGCGGCGGCCGACGCCGCCGCCCGCCCGATCAGTCGCGCACGACGACC
TTGGTACCGACGCGAACCCG

Product: NAD(P)H dehydrogenase (quinone)

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MKLLHLDASILGTGSVSRELSALIVRRLAGDAPDAVTYRDLVAENPPHLTVATLPGAHPVSAMAGPLDAAGQAVRDASDR
MLSEFVAADTVVIGVPMYNFTIPSQLKAWIDRLLVPGTTFRYGAAGPEGLMGGKRVILALARGGFYGPGTASVPAEHAEH
YLRTVFGFMGIVPELVLAEGLAAGEHNKAQALASARDAVGQLAA

Sequences:

>Translated_204_residues
MKLLHLDASILGTGSVSRELSALIVRRLAGDAPDAVTYRDLVAENPPHLTVATLPGAHPVSAMAGPLDAAGQAVRDASDR
MLSEFVAADTVVIGVPMYNFTIPSQLKAWIDRLLVPGTTFRYGAAGPEGLMGGKRVILALARGGFYGPGTASVPAEHAEH
YLRTVFGFMGIVPELVLAEGLAAGEHNKAQALASARDAVGQLAA
>Mature_204_residues
MKLLHLDASILGTGSVSRELSALIVRRLAGDAPDAVTYRDLVAENPPHLTVATLPGAHPVSAMAGPLDAAGQAVRDASDR
MLSEFVAADTVVIGVPMYNFTIPSQLKAWIDRLLVPGTTFRYGAAGPEGLMGGKRVILALARGGFYGPGTASVPAEHAEH
YLRTVFGFMGIVPELVLAEGLAAGEHNKAQALASARDAVGQLAA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=205, Percent_Identity=37.0731707317073, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR_METRJ (B1M4X4)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001757118.1
- ProteinModelPortal:   B1M4X4
- SMR:   B1M4X4
- GeneID:   6140535
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_4469
- HOGENOM:   HBG565834
- OMA:   RTFRYSE
- ProtClustDB:   CLSK2478805
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 21177; Mature: 21177

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLHLDASILGTGSVSRELSALIVRRLAGDAPDAVTYRDLVAENPPHLTVATLPGAHPV
CEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCH
SAMAGPLDAAGQAVRDASDRMLSEFVAADTVVIGVPMYNFTIPSQLKAWIDRLLVPGTTF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCE
RYGAAGPEGLMGGKRVILALARGGFYGPGTASVPAEHAEHYLRTVFGFMGIVPELVLAEG
ECCCCCCCHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAGEHNKAQALASARDAVGQLAA
HCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLLHLDASILGTGSVSRELSALIVRRLAGDAPDAVTYRDLVAENPPHLTVATLPGAHPV
CEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCH
SAMAGPLDAAGQAVRDASDRMLSEFVAADTVVIGVPMYNFTIPSQLKAWIDRLLVPGTTF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCE
RYGAAGPEGLMGGKRVILALARGGFYGPGTASVPAEHAEHYLRTVFGFMGIVPELVLAEG
ECCCCCCCHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAGEHNKAQALASARDAVGQLAA
HCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA