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The map label for this gene is 170750816

Identifier: 170750816

GI number: 170750816

Start: 4705648

End: 4707060

Strand: Direct

Name: 170750816

Synonym: Mrad2831_4427

Alternate gene names: NA

Gene position: 4705648-4707060 (Clockwise)

Preceding gene: 170750815

Following gene: 170750817

Centisome position: 77.42

GC content: 75.94

Gene sequence:

>1413_bases
ATGACGACCGCCGCGACTGACGCAATCTGGCAAGCGCCGATCACCGAGCTCGCGCGGATGATCGCGGCGCGGGAAGTGTC
GAGCGCCGAGCTCGTCGGCGCCAGCCTCGACCGGATCGCGCGGCTCGACGGCGCCCTGAACAGCTTCGTCCACCTCTCGC
CCGCGGCCCTCGACGCCGCGCGCGCGGCCGATGCCGAGATCGCCCGGACGGGTCCGCGCGGCCCGCTGCACGGCATCCCG
CTCGCCATCAAGGACAACTACGGCACCGCCGACATGCCGACGCGGGCCGGCACGGCGGTGGAGGCCCTGACCTTCCCGGC
GGCCGACAGCCACGCCGTGGCGCGACTGCGGGCGGCCGGCGCGGTGATCCTCGGCAAGACGCGCATGCACGAATTCGCCT
GGGGCATGGTCACGCCACCGACCCGCAACCCCTGGGACCCCGACCGGGTGCCCGGCGGCTCCAGCGGCGGCTCGGGCGCG
GCGGTCGCGGCCCGCCTCTGCCCGGCCGCGCTCGGCTCGGACACGGGCGGCTCGATCCGGATCCCGGCCGCCCTGTGCGG
GGTCGTCGGCCTCAAGCCGACCTACGGCCGGGTCGGCCGGTCGGGGATCGTGCCGCATTCCTGGTCCCTCGACCATGCCG
GGCCGCTGACCCTGACGGTGGCCGATTCCGCCCTGATGCTGCAGGCGCTCGCCGGCCCGGATGCCGGCGATCCCGCCGCC
AGCGCGGCGCCGGTCCCCGACTACACGGCCGGGCTGGACCGGCCGATCCGGGGCCTGCGGGTCGCGGTGATCCGCAACCA
CTTCTTCGACGCAATCGGCGACGACGTCGCCGCCGCGATCGAGGCGGCGCTCCGGTTCCTCGAAGACCGGGGCTGCGCGG
TGCGCGACGTCGCGGTGCCGTCCCTGCGCCACGGGCTCGGCGCGATCTACGCCATCGAGCTCGCCTCCTCGACCGCCTAT
CACGGCCGCAGCCTCGCCCAGGGGCACGTGGCGGGCTTCGCGGCCGATGTCCGCGACCTCGTGGAGATGGGCCGGCTCGT
GACCGGGCCGGACTACCTGCGCGCCGAGCAGGCGCGGGCCCTGATCATGGCCGAGATGGCCGGCGTCCTGGCCGAGGCCG
ACGTGATCGTCGGCCCGGCCTCGCCGCTGACCGCGTGGCGGACGGACGAGTCCGCGGTGTCGCTCGGCGGGCGCGCGGAG
AGCGTGCTGGCGGCCTCCTGGCGCCTGACCTACCCGTTCAACCTCGCGGGCCTGCCCGCCATCGCGCTGCCCTGCGGCTT
CGACCGGCGCGGCCTGCCGATCAGCCTCCAGATCGCCGCCCGGCCCTTCGCCGAGGCCGAGATCCTGCGCGTCGCCCACC
AGTACGAGCGGGCGCACGACTGGTGGTCCCGGGTGCCGCCCCTGGCCGGCTGA

Upstream 100 bases:

>100_bases
CCGGTGCTCGCCGAGATCGCGCGGCTGCGCGACCTCGACCTCGCGGACATCCATCCGCCGGTCGTCTTCGATCCGACCTT
GCCGTATCGCCGGGTGCCCC

Downstream 100 bases:

>100_bases
CGCGCCGCCCGGTCCCGCGCAGCTGAGCGGGACCGGGGCGGTCGATTGTACGCAACCAATACCCTATCTAATCGTGCGGA
TCCGCGTGATTTACCGGTCG

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTTAATDAIWQAPITELARMIAAREVSSAELVGASLDRIARLDGALNSFVHLSPAALDAARAADAEIARTGPRGPLHGIP
LAIKDNYGTADMPTRAGTAVEALTFPAADSHAVARLRAAGAVILGKTRMHEFAWGMVTPPTRNPWDPDRVPGGSSGGSGA
AVAARLCPAALGSDTGGSIRIPAALCGVVGLKPTYGRVGRSGIVPHSWSLDHAGPLTLTVADSALMLQALAGPDAGDPAA
SAAPVPDYTAGLDRPIRGLRVAVIRNHFFDAIGDDVAAAIEAALRFLEDRGCAVRDVAVPSLRHGLGAIYAIELASSTAY
HGRSLAQGHVAGFAADVRDLVEMGRLVTGPDYLRAEQARALIMAEMAGVLAEADVIVGPASPLTAWRTDESAVSLGGRAE
SVLAASWRLTYPFNLAGLPAIALPCGFDRRGLPISLQIAARPFAEAEILRVAHQYERAHDWWSRVPPLAG

Sequences:

>Translated_470_residues
MTTAATDAIWQAPITELARMIAAREVSSAELVGASLDRIARLDGALNSFVHLSPAALDAARAADAEIARTGPRGPLHGIP
LAIKDNYGTADMPTRAGTAVEALTFPAADSHAVARLRAAGAVILGKTRMHEFAWGMVTPPTRNPWDPDRVPGGSSGGSGA
AVAARLCPAALGSDTGGSIRIPAALCGVVGLKPTYGRVGRSGIVPHSWSLDHAGPLTLTVADSALMLQALAGPDAGDPAA
SAAPVPDYTAGLDRPIRGLRVAVIRNHFFDAIGDDVAAAIEAALRFLEDRGCAVRDVAVPSLRHGLGAIYAIELASSTAY
HGRSLAQGHVAGFAADVRDLVEMGRLVTGPDYLRAEQARALIMAEMAGVLAEADVIVGPASPLTAWRTDESAVSLGGRAE
SVLAASWRLTYPFNLAGLPAIALPCGFDRRGLPISLQIAARPFAEAEILRVAHQYERAHDWWSRVPPLAG
>Mature_469_residues
TTAATDAIWQAPITELARMIAAREVSSAELVGASLDRIARLDGALNSFVHLSPAALDAARAADAEIARTGPRGPLHGIPL
AIKDNYGTADMPTRAGTAVEALTFPAADSHAVARLRAAGAVILGKTRMHEFAWGMVTPPTRNPWDPDRVPGGSSGGSGAA
VAARLCPAALGSDTGGSIRIPAALCGVVGLKPTYGRVGRSGIVPHSWSLDHAGPLTLTVADSALMLQALAGPDAGDPAAS
AAPVPDYTAGLDRPIRGLRVAVIRNHFFDAIGDDVAAAIEAALRFLEDRGCAVRDVAVPSLRHGLGAIYAIELASSTAYH
GRSLAQGHVAGFAADVRDLVEMGRLVTGPDYLRAEQARALIMAEMAGVLAEADVIVGPASPLTAWRTDESAVSLGGRAES
VLAASWRLTYPFNLAGLPAIALPCGFDRRGLPISLQIAARPFAEAEILRVAHQYERAHDWWSRVPPLAG

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=28.5148514851485, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI195972892, Length=508, Percent_Identity=24.0157480314961, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17537465, Length=516, Percent_Identity=24.4186046511628, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17543272, Length=425, Percent_Identity=28.4705882352941, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17556264, Length=300, Percent_Identity=28, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17556276, Length=198, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=198, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=205, Percent_Identity=30.7317073170732, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=189, Percent_Identity=30.6878306878307, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI32565337, Length=196, Percent_Identity=31.6326530612245, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=452, Percent_Identity=26.3274336283186, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319685, Length=405, Percent_Identity=28.1481481481481, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320448, Length=206, Percent_Identity=30.5825242718447, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648113, Length=460, Percent_Identity=34.3478260869565, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI161078093, Length=502, Percent_Identity=27.6892430278884, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI45550774, Length=498, Percent_Identity=27.1084337349398, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24648435, Length=498, Percent_Identity=27.1084337349398, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24648437, Length=498, Percent_Identity=27.1084337349398, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24644968, Length=515, Percent_Identity=24.6601941747573, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356731, Length=489, Percent_Identity=27.4028629856851, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652985, Length=509, Percent_Identity=25.9332023575639, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922090, Length=509, Percent_Identity=25.9332023575639, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24652981, Length=509, Percent_Identity=25.9332023575639, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24652983, Length=509, Percent_Identity=25.9332023575639, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648441, Length=424, Percent_Identity=27.122641509434, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24648439, Length=424, Percent_Identity=27.122641509434, Blast_Score=111, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 48797; Mature: 48666

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAATDAIWQAPITELARMIAAREVSSAELVGASLDRIARLDGALNSFVHLSPAALDAA
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHH
RAADAEIARTGPRGPLHGIPLAIKDNYGTADMPTRAGTAVEALTFPAADSHAVARLRAAG
HHCCCHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCC
AVILGKTRMHEFAWGMVTPPTRNPWDPDRVPGGSSGGSGAAVAARLCPAALGSDTGGSIR
EEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
IPAALCGVVGLKPTYGRVGRSGIVPHSWSLDHAGPLTLTVADSALMLQALAGPDAGDPAA
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCC
SAAPVPDYTAGLDRPIRGLRVAVIRNHFFDAIGDDVAAAIEAALRFLEDRGCAVRDVAVP
CCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHCCH
SLRHGLGAIYAIELASSTAYHGRSLAQGHVAGFAADVRDLVEMGRLVTGPDYLRAEQARA
HHHHCCCEEEEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LIMAEMAGVLAEADVIVGPASPLTAWRTDESAVSLGGRAESVLAASWRLTYPFNLAGLPA
HHHHHHHHHHHHCCEEECCCCCCCEECCCCHHHHCCCCHHHEEEEEEEEECCCCCCCCCC
IALPCGFDRRGLPISLQIAARPFAEAEILRVAHQYERAHDWWSRVPPLAG
EEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TTAATDAIWQAPITELARMIAAREVSSAELVGASLDRIARLDGALNSFVHLSPAALDAA
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHH
RAADAEIARTGPRGPLHGIPLAIKDNYGTADMPTRAGTAVEALTFPAADSHAVARLRAAG
HHCCCHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCC
AVILGKTRMHEFAWGMVTPPTRNPWDPDRVPGGSSGGSGAAVAARLCPAALGSDTGGSIR
EEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
IPAALCGVVGLKPTYGRVGRSGIVPHSWSLDHAGPLTLTVADSALMLQALAGPDAGDPAA
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCC
SAAPVPDYTAGLDRPIRGLRVAVIRNHFFDAIGDDVAAAIEAALRFLEDRGCAVRDVAVP
CCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHCCH
SLRHGLGAIYAIELASSTAYHGRSLAQGHVAGFAADVRDLVEMGRLVTGPDYLRAEQARA
HHHHCCCEEEEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LIMAEMAGVLAEADVIVGPASPLTAWRTDESAVSLGGRAESVLAASWRLTYPFNLAGLPA
HHHHHHHHHHHHCCEEECCCCCCCEECCCCHHHHCCCCHHHEEEEEEEEECCCCCCCCCC
IALPCGFDRRGLPISLQIAARPFAEAEILRVAHQYERAHDWWSRVPPLAG
EEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9809753 [H]