Click here to switch to the map view.

The map label for this gene is yafV [C]

Identifier: 170750810

GI number: 170750810

Start: 4698821

End: 4699666

Strand: Direct

Name: yafV [C]

Synonym: Mrad2831_4421

Alternate gene names: 170750810

Gene position: 4698821-4699666 (Clockwise)

Preceding gene: 170750809

Following gene: 170750811

Centisome position: 77.31

GC content: 71.87

Gene sequence:

>846_bases
ATGATCCAGGCGAACGGTCCTGTGCGCCCGCCGATACGGGTGTCGTGCATCCAGTTCGAGCCGGAATTCGGCGCGGTCGA
CGCGAATATGGCGCGCGCGTCGGACCTCGTCCGCGCGGCCGCCGCCGAGGGCAGCCGGCTGATCGTGCTGCCGGAACTCG
CCAGCACCGGCTACGTGTTCGAATCCGCCGCGGAGGCCGCGGCCCTGGCCGAGCCGGTGCCGGACGGCCCGACGACCCGG
GCCTGGGCGGCCCTCGCCGCGGAACTCGGCGTCCACATCGTGGCGGGGATCGCCGAATCCGCGGGCGAGATCCTGTACAA
CGCCGCCGTGATCGTCGGGCCGGGGGGCTATATCGGCACCTACCGCAAGGCGCATCTCTGGGACCAGGAGAACGTGTTCT
TCGCCAGGGGCGACCTCGGCTTCCCGGTCTTCGACACCGCGCTCGGCAAGGTCGGCGTCGCCATCTGCTACGACGGCTGG
TTTCCCGAGACCTTCCGGCGGCTGGCGCTCGCCGGCGCCGAGATCGTCTGCATCCCGACCAACTGGGTGCCGATGCCCGA
CCAGCCCGAGGGCGAGGCCGCCATGGCCAACACCCTGCACCGGGCCGCCGCCCACACGAACGGCATCTTCATCGCCTGCG
CCGACCGGGTCGGCATCGAGCGCGGCCAGCCCTTCGAGGGGCAGAGCCTGATCATCGGCCCCAAGGGCTGGCCGCTCGCC
GGCCCGGCGAGCCGGGACCGGACCGAGACCCTCTCGGCGCTGGTCGACCTCGACGCGGCAGGCCGCAAGGACCTGAACCG
CTTCAACTCGCTCCTGCGCGACCGCCGCACCGACATCTACGGTTGA

Upstream 100 bases:

>100_bases
GCCCCGCAGCAGCAGTGCTGCATGACATCACGGCTTTGAAGCCTCTGGTTCGCGGATGTTTGTATCCGCGCCCAGGGGCT
TTTTGCGTTTCGGAGCTTTC

Downstream 100 bases:

>100_bases
GCCGAGATCCTTACCCACCCCCGTCCGTCCCAAGGAGTTCGTTCATGGTGAAATGGTCCCTCGTCGGCGCCGCGGCGCTC
GCCCTCGCCGCCACGGCCGC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MIQANGPVRPPIRVSCIQFEPEFGAVDANMARASDLVRAAAAEGSRLIVLPELASTGYVFESAAEAAALAEPVPDGPTTR
AWAALAAELGVHIVAGIAESAGEILYNAAVIVGPGGYIGTYRKAHLWDQENVFFARGDLGFPVFDTALGKVGVAICYDGW
FPETFRRLALAGAEIVCIPTNWVPMPDQPEGEAAMANTLHRAAAHTNGIFIACADRVGIERGQPFEGQSLIIGPKGWPLA
GPASRDRTETLSALVDLDAAGRKDLNRFNSLLRDRRTDIYG

Sequences:

>Translated_281_residues
MIQANGPVRPPIRVSCIQFEPEFGAVDANMARASDLVRAAAAEGSRLIVLPELASTGYVFESAAEAAALAEPVPDGPTTR
AWAALAAELGVHIVAGIAESAGEILYNAAVIVGPGGYIGTYRKAHLWDQENVFFARGDLGFPVFDTALGKVGVAICYDGW
FPETFRRLALAGAEIVCIPTNWVPMPDQPEGEAAMANTLHRAAAHTNGIFIACADRVGIERGQPFEGQSLIIGPKGWPLA
GPASRDRTETLSALVDLDAAGRKDLNRFNSLLRDRRTDIYG
>Mature_281_residues
MIQANGPVRPPIRVSCIQFEPEFGAVDANMARASDLVRAAAAEGSRLIVLPELASTGYVFESAAEAAALAEPVPDGPTTR
AWAALAAELGVHIVAGIAESAGEILYNAAVIVGPGGYIGTYRKAHLWDQENVFFARGDLGFPVFDTALGKVGVAICYDGW
FPETFRRLALAGAEIVCIPTNWVPMPDQPEGEAAMANTLHRAAAHTNGIFIACADRVGIERGQPFEGQSLIIGPKGWPLA
GPASRDRTETLSALVDLDAAGRKDLNRFNSLLRDRRTDIYG

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=283, Percent_Identity=28.2685512367491, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI7706509, Length=263, Percent_Identity=28.8973384030418, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI5031947, Length=272, Percent_Identity=29.4117647058824, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI297632348, Length=275, Percent_Identity=29.4545454545455, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI297632350, Length=274, Percent_Identity=29.5620437956204, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI297632346, Length=169, Percent_Identity=34.3195266272189, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17533173, Length=299, Percent_Identity=31.1036789297659, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17557111, Length=172, Percent_Identity=28.4883720930233, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323383, Length=287, Percent_Identity=24.7386759581882, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21358471, Length=253, Percent_Identity=28.4584980237154, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355835, Length=285, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 29869; Mature: 29869

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQANGPVRPPIRVSCIQFEPEFGAVDANMARASDLVRAAAAEGSRLIVLPELASTGYVF
CCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHH
ESAAEAAALAEPVPDGPTTRAWAALAAELGVHIVAGIAESAGEILYNAAVIVGPGGYIGT
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCC
YRKAHLWDQENVFFARGDLGFPVFDTALGKVGVAICYDGWFPETFRRLALAGAEIVCIPT
CCHHEECCCCCEEEEECCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCEEEECC
NWVPMPDQPEGEAAMANTLHRAAAHTNGIFIACADRVGIERGQPFEGQSLIIGPKGWPLA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCC
GPASRDRTETLSALVDLDAAGRKDLNRFNSLLRDRRTDIYG
CCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIQANGPVRPPIRVSCIQFEPEFGAVDANMARASDLVRAAAAEGSRLIVLPELASTGYVF
CCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHH
ESAAEAAALAEPVPDGPTTRAWAALAAELGVHIVAGIAESAGEILYNAAVIVGPGGYIGT
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCC
YRKAHLWDQENVFFARGDLGFPVFDTALGKVGVAICYDGWFPETFRRLALAGAEIVCIPT
CCHHEECCCCCEEEEECCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCEEEECC
NWVPMPDQPEGEAAMANTLHRAAAHTNGIFIACADRVGIERGQPFEGQSLIIGPKGWPLA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCC
GPASRDRTETLSALVDLDAAGRKDLNRFNSLLRDRRTDIYG
CCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]