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The map label for this gene is gsiB [H]

Identifier: 170750770

GI number: 170750770

Start: 4646494

End: 4648026

Strand: Reverse

Name: gsiB [H]

Synonym: Mrad2831_4381

Alternate gene names: 170750770

Gene position: 4648026-4646494 (Counterclockwise)

Preceding gene: 170750773

Following gene: 170750769

Centisome position: 76.48

GC content: 68.49

Gene sequence:

>1533_bases
ATGCCGCTACGGACTCTCACCCGGGCCGCCACCCTGGCCGCCTCGCTCGCCGCACTGATGGCCGCGCACCCGGCGCTCGC
CGGCAAGGCCGACGACACGCTGGTCTACGCCTCCGACAGCGAGCCCGAGAACGTCAGCCCGTACCACAACAGCGCCCGCG
AGGGCGTGATCCTGGCCCGGAACTGCTGGGACACGCTGCTGTACCGGGATCCGAAGGACGGCACCTACAAGCCGATGCTG
GCGACCGCCTGGACATGGGCGGACGACACGACCCTCGACCTCACGATCCGCGAGGGCGTGGTCTTCCACAACGGCGACCC
GCTCGGGCCCGAGGACGTGGCCTTCACGTTCAACTACGTGCTGACCCCCGAGGCGCGCACGGTCACCCGCCAGAACGTCG
ACTGGATGAAGTCGGTGGAGGTGACCGGCCCGCACAGCGTGCGCATCCACCTCAAGGCGCCGTTCCCGGCCGCGCTCGAG
TACCTCGCCGGCCCGACCCCGATCTTCCCGGCGAACTACTTCAAGAAGGTCGGCCTCGACGGCTTCGCCAAGGCGCCGGT
CGGCAGCGGACCCTACCGGATCACCAAGGTCGACAGCGGCCGCGGCGTCAGCATGGAGCGGTTCGACAAGTACTGGGCGG
AGAGCCCTCTGGGTAAGCCGAAGATCGGCAAGCTGCAGTTCCGCGTCATCCCGGACGGCGAGAGCCGGATGGCCGAGCTG
ATGACCGGCGGCGTCGACTGGATCTGGCGGGTGCCGAGCGACCAGGCCGAGCAGCTGCGCGCCGCGCCCAACATCACGGT
CCTCAATTCCGAGACGATGCGGGTCGGCTTCCTGCAGTTCGACACGCTCGGCCGCGCCAAGGAGAACTCGCCGCTCAAGG
ACGTGCGCGTCCGGCAGGCGATCTCCTACGCCATCGACCGCAAGGCGATGGTCGACAACCTCGTGCGCGGCGCCTCGCGG
GTGATGAACGCGCTGTGCTTCGTCGACCAGTTCGGCTGCACCGACCAGGGGGTGATGCGCTACCCCTACGACCCCGCCAA
GGCCAAGGCGCTGCTCAAGGAAGCCGGCTACGGAAACGGCTTCGAGATCGACCTCGGCGCCTATCGCGAGCGCGACTACG
CCGAGGCGGTGATCGGCTATCTCGGCGCCGTCGGCATCAAGGCGCGGCTCAACTACCTGCGCTACGCGGCGTTCCGCGAC
GCGCTGCGGGCCGGCAAGACCTCGATCGGCTACCAGACCTGGGGCTCGTTCTCGGTGCTGGACGTCTCGGCCTTCGACGG
GGTGTATTTCCGCGGTGGCGAGGAGGATCTGACCAAGGATCCGCAGGTAATCGCGGATCTGCAGAAGGGCGACGGCTCGA
CCGACCCGGAGACGCGCAAGGCCGCCTACGCCAAGGCGCTCCAGCGGATCGCCGCGGAAGCCTACGCGCTGCCGATGTTC
TCCTACTCGACCAACTACGCCTTCACGTCGGACCTCAACTTCACCGCCCAGCCCGACGAGGTGCCGCGCTTCTACGCGGC
GTCGTGGAAGTGA

Upstream 100 bases:

>100_bases
ACTTGTTGCGATCCCAGATGCACAACACCATGGCAGGGTGAAAAACGCGGCACCCGCGGAACCGTCCGCCGGGCCTGTCC
GCCGCGCCGTCGGAGCCTGC

Downstream 100 bases:

>100_bases
CGGCGGTGGCCGCGGCGCGCCGAGCCCCCTCTCCCGTGCGGGAGCGGGTTGGGGTGAGGGGCCCGGTCAGTCCGGAGAGG
TCGCATCCCTCACCCTGTCC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MPLRTLTRAATLAASLAALMAAHPALAGKADDTLVYASDSEPENVSPYHNSAREGVILARNCWDTLLYRDPKDGTYKPML
ATAWTWADDTTLDLTIREGVVFHNGDPLGPEDVAFTFNYVLTPEARTVTRQNVDWMKSVEVTGPHSVRIHLKAPFPAALE
YLAGPTPIFPANYFKKVGLDGFAKAPVGSGPYRITKVDSGRGVSMERFDKYWAESPLGKPKIGKLQFRVIPDGESRMAEL
MTGGVDWIWRVPSDQAEQLRAAPNITVLNSETMRVGFLQFDTLGRAKENSPLKDVRVRQAISYAIDRKAMVDNLVRGASR
VMNALCFVDQFGCTDQGVMRYPYDPAKAKALLKEAGYGNGFEIDLGAYRERDYAEAVIGYLGAVGIKARLNYLRYAAFRD
ALRAGKTSIGYQTWGSFSVLDVSAFDGVYFRGGEEDLTKDPQVIADLQKGDGSTDPETRKAAYAKALQRIAAEAYALPMF
SYSTNYAFTSDLNFTAQPDEVPRFYAASWK

Sequences:

>Translated_510_residues
MPLRTLTRAATLAASLAALMAAHPALAGKADDTLVYASDSEPENVSPYHNSAREGVILARNCWDTLLYRDPKDGTYKPML
ATAWTWADDTTLDLTIREGVVFHNGDPLGPEDVAFTFNYVLTPEARTVTRQNVDWMKSVEVTGPHSVRIHLKAPFPAALE
YLAGPTPIFPANYFKKVGLDGFAKAPVGSGPYRITKVDSGRGVSMERFDKYWAESPLGKPKIGKLQFRVIPDGESRMAEL
MTGGVDWIWRVPSDQAEQLRAAPNITVLNSETMRVGFLQFDTLGRAKENSPLKDVRVRQAISYAIDRKAMVDNLVRGASR
VMNALCFVDQFGCTDQGVMRYPYDPAKAKALLKEAGYGNGFEIDLGAYRERDYAEAVIGYLGAVGIKARLNYLRYAAFRD
ALRAGKTSIGYQTWGSFSVLDVSAFDGVYFRGGEEDLTKDPQVIADLQKGDGSTDPETRKAAYAKALQRIAAEAYALPMF
SYSTNYAFTSDLNFTAQPDEVPRFYAASWK
>Mature_509_residues
PLRTLTRAATLAASLAALMAAHPALAGKADDTLVYASDSEPENVSPYHNSAREGVILARNCWDTLLYRDPKDGTYKPMLA
TAWTWADDTTLDLTIREGVVFHNGDPLGPEDVAFTFNYVLTPEARTVTRQNVDWMKSVEVTGPHSVRIHLKAPFPAALEY
LAGPTPIFPANYFKKVGLDGFAKAPVGSGPYRITKVDSGRGVSMERFDKYWAESPLGKPKIGKLQFRVIPDGESRMAELM
TGGVDWIWRVPSDQAEQLRAAPNITVLNSETMRVGFLQFDTLGRAKENSPLKDVRVRQAISYAIDRKAMVDNLVRGASRV
MNALCFVDQFGCTDQGVMRYPYDPAKAKALLKEAGYGNGFEIDLGAYRERDYAEAVIGYLGAVGIKARLNYLRYAAFRDA
LRAGKTSIGYQTWGSFSVLDVSAFDGVYFRGGEEDLTKDPQVIADLQKGDGSTDPETRKAAYAKALQRIAAEAYALPMFS
YSTNYAFTSDLNFTAQPDEVPRFYAASWK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=401, Percent_Identity=29.6758104738155, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1789887, Length=439, Percent_Identity=26.4236902050114, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789966, Length=445, Percent_Identity=26.0674157303371, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1787551, Length=386, Percent_Identity=25.3886010362694, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787762, Length=520, Percent_Identity=23.6538461538462, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082063, Length=176, Percent_Identity=32.3863636363636, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789397, Length=487, Percent_Identity=23.2032854209446, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56304; Mature: 56173

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRTLTRAATLAASLAALMAAHPALAGKADDTLVYASDSEPENVSPYHNSAREGVILAR
CCCHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEH
NCWDTLLYRDPKDGTYKPMLATAWTWADDTTLDLTIREGVVFHNGDPLGPEDVAFTFNYV
HHHHHHHEECCCCCCCCCEEEEEEECCCCCEEEEEEECCEEEECCCCCCCCCEEEEEEEE
LTPEARTVTRQNVDWMKSVEVTGPHSVRIHLKAPFPAALEYLAGPTPIFPANYFKKVGLD
ECCCHHHHHHCCCCHHHEEEECCCCEEEEEEECCCHHHHHHHCCCCCCCCHHHHHHCCCC
GFAKAPVGSGPYRITKVDSGRGVSMERFDKYWAESPLGKPKIGKLQFRVIPDGESRMAEL
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHH
MTGGVDWIWRVPSDQAEQLRAAPNITVLNSETMRVGFLQFDTLGRAKENSPLKDVRVRQA
HHCCCCEEEECCCCHHHHHHCCCCEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHH
ISYAIDRKAMVDNLVRGASRVMNALCFVDQFGCTDQGVMRYPYDPAKAKALLKEAGYGNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHCCCCCC
FEIDLGAYRERDYAEAVIGYLGAVGIKARLNYLRYAAFRDALRAGKTSIGYQTWGSFSVL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
DVSAFDGVYFRGGEEDLTKDPQVIADLQKGDGSTDPETRKAAYAKALQRIAAEAYALPMF
EEECCCCEEEECCCHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEE
SYSTNYAFTSDLNFTAQPDEVPRFYAASWK
ECCCCEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLRTLTRAATLAASLAALMAAHPALAGKADDTLVYASDSEPENVSPYHNSAREGVILAR
CCHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEH
NCWDTLLYRDPKDGTYKPMLATAWTWADDTTLDLTIREGVVFHNGDPLGPEDVAFTFNYV
HHHHHHHEECCCCCCCCCEEEEEEECCCCCEEEEEEECCEEEECCCCCCCCCEEEEEEEE
LTPEARTVTRQNVDWMKSVEVTGPHSVRIHLKAPFPAALEYLAGPTPIFPANYFKKVGLD
ECCCHHHHHHCCCCHHHEEEECCCCEEEEEEECCCHHHHHHHCCCCCCCCHHHHHHCCCC
GFAKAPVGSGPYRITKVDSGRGVSMERFDKYWAESPLGKPKIGKLQFRVIPDGESRMAEL
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHH
MTGGVDWIWRVPSDQAEQLRAAPNITVLNSETMRVGFLQFDTLGRAKENSPLKDVRVRQA
HHCCCCEEEECCCCHHHHHHCCCCEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHH
ISYAIDRKAMVDNLVRGASRVMNALCFVDQFGCTDQGVMRYPYDPAKAKALLKEAGYGNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHCCCCCC
FEIDLGAYRERDYAEAVIGYLGAVGIKARLNYLRYAAFRDALRAGKTSIGYQTWGSFSVL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
DVSAFDGVYFRGGEEDLTKDPQVIADLQKGDGSTDPETRKAAYAKALQRIAAEAYALPMF
EEECCCCEEEECCCHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEE
SYSTNYAFTSDLNFTAQPDEVPRFYAASWK
ECCCCEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA