BacMap

Definition	Methylobacterium radiotolerans JCM 2831 chromosome, complete genome.
Accession	NC_010505
Length	6,077,833

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The map label for this gene is yngJ [H]

Identifier: 170750740

GI number: 170750740

Start: 4611178

End: 4612860

Strand: Direct

Name: yngJ [H]

Synonym: Mrad2831_4351

Alternate gene names: 170750740

Gene position: 4611178-4612860 (Clockwise)

Preceding gene: 170750739

Following gene: 170750741

Centisome position: 75.87

GC content: 70.47

Gene sequence:

>1683_bases
ATGTCCGCATCCCCCGCCATCCAGACCGAGCCGCAGGATCTCGTGTCCCTGACCAAGGCCGCCGGTGAGAGCGCGAAAGC
GCTGGCCGCCGAGGCCACGCGCCGGGTCCGCACGCGGATCCTCGGGACGGACGGGCGGATCGACGCGGCCAAGCTCGAGG
GCCAGCAGCACGCCGCCCACGGGCTCGCCTGGCTCGCGACCTACGCGGAGGCGGTGAGCGAGCTCGGCGGCTACGCCGAG
CGACTCCAGGCCGAGGGCCGGTTCGGCGAGACCGAGGCGCTCCTCGTCCGGATCGGCGCCGGCGAGTATCTCGACCAGCT
CTTCTCCGGCATCCCGATGAGTCAGGGCGAGATGGTCCGCCTGACCGGGTCGCTCGGCCTCTCCGCCAAGGAGGTGGCCC
AGTACCGCACGGACGCGGTCGAGACCCTGATCGCCGAGGGCAACACCCCCGAGAACCGCGCCGCCCTGGTGGCGCTGATG
CGCGACGGCGGCGGCTCGGCGACCGTCGGCGCCTCGGGCCTCGACGAGGACATGGAGGCGATCCGCTCCGAGATGCGCCG
CTTCGGCAAGGCCGAGGTGGTCGATCAGGCGCACGAGTGGCACCTGAAGAACGCCTACATCCCGATGGAGATCGTGCAGA
AGATGGCCGAGCTCGGCGTCTTCGGCCTGACGATCCCCGAGGAGTACGGCGGCATGGGCCTGCCGAAGACCGCCATGTGC
GTGGTCTCGGAGGAACTGTCCCGGGCCTATATCGGCGTCGGCTCGCTCGGCACCCGCTCGGAGATCGCCGCGGAGCTGAT
CCTGTGCGGCGGCACCGAGGAGCAGAAGCAGCGCTTCCTGCCGAAGATCGCCAGCGGCGACATTCTTCCGACGGCCGTCT
TCACCGAGCCCAATACCGGCTCCGACCTCGCGAGCCTGCGCACCCGCGCGGTGAAGGAGGGCGACGTCTGGAAGATCACC
GGCAACAAGACCTGGATCACCCACCCGGTCCGCGCCGACATCATGACGGTGCTCGTCCGCACCAAGCCCGAGGAGAAGGG
CCACAAGGGCCTCTCCATGCTGATCGCCGAGAAGCCGCGCGGCACCGACGAGGACCCGTTCCCAGTCCAGGGCCTGTCGG
GCGGCGAGATCGAGGTGCTCGGCTACCGCGGCATGAAGGAGTACGAGCTCGCCTTCGAGGGCTTCGCGGTGCCGGGCGAC
AACCTGCTGGGCGAGGTCGAGGGCAAGGGCTTCGCCCAGCTGATGCAGACCTTCGAGTCGGCCCGCATCCAGACCGCGGC
GCGCGCCATCGGCGTCGCCCAGTCGGCCCTCGATGTCGGCCTGCGCTACGCCGAGGAGCGGGTCCAGTTCGGCAAGGCGC
TGGTGAACTTCCCGCGGGTGGCCGACAAGCTCGCCATGATGGCGGTGGAGATCAACATCGCCCGCCAGCTCACCTACTTC
TCGGCCCGCGAGAAGGACGAGGGCAAGCGCTGCGACCTGGAGGCCGGGATGGCGAAGCTGCTCGGCGCCCGCGTCGCCTG
GGCGGCGGCCGACAACGCCCTCCAGATCCACGGCGGCAACGGCTTCGCGCTCGAGTACCCGGTCAGCCGGATCCTGTGCG
ACGCGCGCATCCTCTCGATCTTCGAGGGCGCCGCGGAGATCCAGGCCCAGGTGATCGCCCGCCGGCTGCTCGAATCCGCC
TGA

Upstream 100 bases:

>100_bases
CAACTTGAGTTCAAGACGAGCCAAACGAAATCTGCTTCGGCTCCGGACCGCGACAGGTTAAAAGACCCGATCCAAGAAGA
CGCCACGCGAGCAGAAAACC

Downstream 100 bases:

>100_bases
AGACTCGGCTAAAACTGGGGAATAGTACGTAACATGGGATTATAAGTCGGCTCATGAACCCGGCTTAAACGAAGTCCCCG
CTTCCTGGCTCCGGTGAACA

Product: acyl-CoA dehydrogenase domain-containing protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MSASPAIQTEPQDLVSLTKAAGESAKALAAEATRRVRTRILGTDGRIDAAKLEGQQHAAHGLAWLATYAEAVSELGGYAE
RLQAEGRFGETEALLVRIGAGEYLDQLFSGIPMSQGEMVRLTGSLGLSAKEVAQYRTDAVETLIAEGNTPENRAALVALM
RDGGGSATVGASGLDEDMEAIRSEMRRFGKAEVVDQAHEWHLKNAYIPMEIVQKMAELGVFGLTIPEEYGGMGLPKTAMC
VVSEELSRAYIGVGSLGTRSEIAAELILCGGTEEQKQRFLPKIASGDILPTAVFTEPNTGSDLASLRTRAVKEGDVWKIT
GNKTWITHPVRADIMTVLVRTKPEEKGHKGLSMLIAEKPRGTDEDPFPVQGLSGGEIEVLGYRGMKEYELAFEGFAVPGD
NLLGEVEGKGFAQLMQTFESARIQTAARAIGVAQSALDVGLRYAEERVQFGKALVNFPRVADKLAMMAVEINIARQLTYF
SAREKDEGKRCDLEAGMAKLLGARVAWAAADNALQIHGGNGFALEYPVSRILCDARILSIFEGAAEIQAQVIARRLLESA

Sequences:

>Translated_560_residues
MSASPAIQTEPQDLVSLTKAAGESAKALAAEATRRVRTRILGTDGRIDAAKLEGQQHAAHGLAWLATYAEAVSELGGYAE
RLQAEGRFGETEALLVRIGAGEYLDQLFSGIPMSQGEMVRLTGSLGLSAKEVAQYRTDAVETLIAEGNTPENRAALVALM
RDGGGSATVGASGLDEDMEAIRSEMRRFGKAEVVDQAHEWHLKNAYIPMEIVQKMAELGVFGLTIPEEYGGMGLPKTAMC
VVSEELSRAYIGVGSLGTRSEIAAELILCGGTEEQKQRFLPKIASGDILPTAVFTEPNTGSDLASLRTRAVKEGDVWKIT
GNKTWITHPVRADIMTVLVRTKPEEKGHKGLSMLIAEKPRGTDEDPFPVQGLSGGEIEVLGYRGMKEYELAFEGFAVPGD
NLLGEVEGKGFAQLMQTFESARIQTAARAIGVAQSALDVGLRYAEERVQFGKALVNFPRVADKLAMMAVEINIARQLTYF
SAREKDEGKRCDLEAGMAKLLGARVAWAAADNALQIHGGNGFALEYPVSRILCDARILSIFEGAAEIQAQVIARRLLESA
>Mature_559_residues
SASPAIQTEPQDLVSLTKAAGESAKALAAEATRRVRTRILGTDGRIDAAKLEGQQHAAHGLAWLATYAEAVSELGGYAER
LQAEGRFGETEALLVRIGAGEYLDQLFSGIPMSQGEMVRLTGSLGLSAKEVAQYRTDAVETLIAEGNTPENRAALVALMR
DGGGSATVGASGLDEDMEAIRSEMRRFGKAEVVDQAHEWHLKNAYIPMEIVQKMAELGVFGLTIPEEYGGMGLPKTAMCV
VSEELSRAYIGVGSLGTRSEIAAELILCGGTEEQKQRFLPKIASGDILPTAVFTEPNTGSDLASLRTRAVKEGDVWKITG
NKTWITHPVRADIMTVLVRTKPEEKGHKGLSMLIAEKPRGTDEDPFPVQGLSGGEIEVLGYRGMKEYELAFEGFAVPGDN
LLGEVEGKGFAQLMQTFESARIQTAARAIGVAQSALDVGLRYAEERVQFGKALVNFPRVADKLAMMAVEINIARQLTYFS
AREKDEGKRCDLEAGMAKLLGARVAWAAADNALQIHGGNGFALEYPVSRILCDARILSIFEGAAEIQAQVIARRLLESA

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=391, Percent_Identity=37.8516624040921, Blast_Score=213, Evalue=5e-55,
Organism=Homo sapiens, GI4557231, Length=389, Percent_Identity=33.6760925449871, Blast_Score=207, Evalue=3e-53,
Organism=Homo sapiens, GI187960098, Length=389, Percent_Identity=33.6760925449871, Blast_Score=207, Evalue=3e-53,
Organism=Homo sapiens, GI21361497, Length=362, Percent_Identity=36.1878453038674, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI226958412, Length=387, Percent_Identity=32.2997416020672, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI76496475, Length=344, Percent_Identity=34.593023255814, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI4557235, Length=344, Percent_Identity=34.593023255814, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI4501859, Length=384, Percent_Identity=31.5104166666667, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI226958414, Length=348, Percent_Identity=33.3333333333333, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI4501857, Length=384, Percent_Identity=28.90625, Blast_Score=169, Evalue=9e-42,
Organism=Homo sapiens, GI7656849, Length=388, Percent_Identity=30.9278350515464, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4503943, Length=362, Percent_Identity=30.1104972375691, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI7669494, Length=350, Percent_Identity=30, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI38505218, Length=359, Percent_Identity=26.1838440111421, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI48976061, Length=298, Percent_Identity=29.5302013422819, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI211971008, Length=298, Percent_Identity=29.5302013422819, Blast_Score=91, Evalue=4e-18,
Organism=Escherichia coli, GI1786223, Length=364, Percent_Identity=27.4725274725275, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI87081958, Length=386, Percent_Identity=27.2020725388601, Blast_Score=138, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17570075, Length=388, Percent_Identity=36.0824742268041, Blast_Score=223, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17534899, Length=389, Percent_Identity=35.9897172236504, Blast_Score=211, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI17569725, Length=391, Percent_Identity=35.8056265984655, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17538396, Length=386, Percent_Identity=30.5699481865285, Blast_Score=200, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71985184, Length=355, Percent_Identity=33.5211267605634, Blast_Score=187, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17506239, Length=372, Percent_Identity=33.3333333333333, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17508101, Length=383, Percent_Identity=32.1148825065274, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17533517, Length=388, Percent_Identity=31.1855670103093, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17551932, Length=392, Percent_Identity=29.3367346938776, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17505929, Length=396, Percent_Identity=28.7878787878788, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71990804, Length=295, Percent_Identity=32.8813559322034, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71985192, Length=335, Percent_Identity=29.8507462686567, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17534353, Length=385, Percent_Identity=26.2337662337662, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32563615, Length=238, Percent_Identity=32.7731092436975, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71982178, Length=286, Percent_Identity=27.972027972028, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17562446, Length=376, Percent_Identity=26.5957446808511, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17531909, Length=252, Percent_Identity=26.5873015873016, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24660351, Length=391, Percent_Identity=35.2941176470588, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI21356377, Length=392, Percent_Identity=34.1836734693878, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24646207, Length=396, Percent_Identity=33.5858585858586, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI21355753, Length=390, Percent_Identity=33.5897435897436, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI281363737, Length=347, Percent_Identity=33.4293948126801, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24666513, Length=383, Percent_Identity=31.0704960835509, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI19920834, Length=390, Percent_Identity=29.2307692307692, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 60201; Mature: 60070

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASPAIQTEPQDLVSLTKAAGESAKALAAEATRRVRTRILGTDGRIDAAKLEGQQHAAH
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCHHHHHH
GLAWLATYAEAVSELGGYAERLQAEGRFGETEALLVRIGAGEYLDQLFSGIPMSQGEMVR
HHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCEEE
LTGSLGLSAKEVAQYRTDAVETLIAEGNTPENRAALVALMRDGGGSATVGASGLDEDMEA
EECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEECCCCCCCHHHHH
IRSEMRRFGKAEVVDQAHEWHLKNAYIPMEIVQKMAELGVFGLTIPEEYGGMGLPKTAMC
HHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCHHHCCCCCCHHHHH
VVSEELSRAYIGVGSLGTRSEIAAELILCGGTEEQKQRFLPKIASGDILPTAVFTEPNTG
HHHHHHHHHHCCCCCCCCHHHHHHHHHEECCCHHHHHHHCCHHCCCCCCCEEEEECCCCC
SDLASLRTRAVKEGDVWKITGNKTWITHPVRADIMTVLVRTKPEEKGHKGLSMLIAEKPR
CHHHHHHHHHCCCCCEEEEECCCEEEECCHHHHHHHHHHHCCCHHCCCCCCEEEEECCCC
GTDEDPFPVQGLSGGEIEVLGYRGMKEYELAFEGFAVPGDNLLGEVEGKGFAQLMQTFES
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHH
ARIQTAARAIGVAQSALDVGLRYAEERVQFGKALVNFPRVADKLAMMAVEINIARQLTYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAREKDEGKRCDLEAGMAKLLGARVAWAAADNALQIHGGNGFALEYPVSRILCDARILSI
HCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCEEEEECCCCEEEECCHHHHHHHHHHHHH
FEGAAEIQAQVIARRLLESA
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SASPAIQTEPQDLVSLTKAAGESAKALAAEATRRVRTRILGTDGRIDAAKLEGQQHAAH
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCHHHHHH
GLAWLATYAEAVSELGGYAERLQAEGRFGETEALLVRIGAGEYLDQLFSGIPMSQGEMVR
HHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCEEE
LTGSLGLSAKEVAQYRTDAVETLIAEGNTPENRAALVALMRDGGGSATVGASGLDEDMEA
EECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEECCCCCCCHHHHH
IRSEMRRFGKAEVVDQAHEWHLKNAYIPMEIVQKMAELGVFGLTIPEEYGGMGLPKTAMC
HHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCHHHCCCCCCHHHHH
VVSEELSRAYIGVGSLGTRSEIAAELILCGGTEEQKQRFLPKIASGDILPTAVFTEPNTG
HHHHHHHHHHCCCCCCCCHHHHHHHHHEECCCHHHHHHHCCHHCCCCCCCEEEEECCCCC
SDLASLRTRAVKEGDVWKITGNKTWITHPVRADIMTVLVRTKPEEKGHKGLSMLIAEKPR
CHHHHHHHHHCCCCCEEEEECCCEEEECCHHHHHHHHHHHCCCHHCCCCCCEEEEECCCC
GTDEDPFPVQGLSGGEIEVLGYRGMKEYELAFEGFAVPGDNLLGEVEGKGFAQLMQTFES
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHH
ARIQTAARAIGVAQSALDVGLRYAEERVQFGKALVNFPRVADKLAMMAVEINIARQLTYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAREKDEGKRCDLEAGMAKLLGARVAWAAADNALQIHGGNGFALEYPVSRILCDARILSI
HCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCEEEEECCCCEEEECCHHHHHHHHHHHHH
FEGAAEIQAQVIARRLLESA
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]