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The map label for this gene is kefA [H]

Identifier: 170750732

GI number: 170750732

Start: 4600097

End: 4602829

Strand: Direct

Name: kefA [H]

Synonym: Mrad2831_4343

Alternate gene names: 170750732

Gene position: 4600097-4602829 (Clockwise)

Preceding gene: 170750730

Following gene: 170750734

Centisome position: 75.69

GC content: 71.68

Gene sequence:

>2733_bases
ATGCATCGTATCCCAGCGCGGACCCGGAAGCCGGAGGGCGCCCCGCGCCCGTCCCGCACCCTCGCCCTGCTCGCCGCGCT
GGTGTTCGCAACCCCGGCGGCCCTGGCCCTGCCGGCCTTCGCGCAGGATCCGGCGCCCGCCGCCAAGTCCGCCGATCAGA
AACCCGCGGATCACAAGCCTGCGGACACCAAGGCCGCCGACACCAAGGCCGCGCCCGAGGCCAAGCCCGCGGACGGCAAG
GCACCCGACGCGCAGGCGACCGGCGCGAGACCGGCCGACGCGAAGCCCGCGCCCCCGCCGGCCGCGAAGGCCGAGGTTCC
CGAGCAGTTCAAGAAGGTCCGGGCCAAGCTCGACGCCGCCAAGGCCGAGCTCGACGCCCGCGAGAAGCAGCTCGGCCACG
CGGAGCTGACGGTCGTCGACCTTAACGCCATCCGCGACAGCGCCGTCACGGTCACCGACGAGATCCGCACCCTGGTCACC
CGGCTCGACGCGCCGCTGGAGGCCGCGCGCGAGCGCCTGGCCCAGCTCGGGCCGAAGCCGAAGGACACCCAGGAGAGCGC
CGACGTCGCCGCGGAGCGCGCCGAGCGCGAGGCCGCCGTCACCCGCATCGACGAGACCCAGCGCCTCGCCCGCTCCCTGG
TGGTGCAGGGCGACCAGATCGTCGACCGGGTCTCGAACCGCCGCCGCGAATCCTTCACCCGCGACCTGCTCCAGCGCTCC
CAGCCGCTGGTCGGCCCCGGTCTCTGGACCAAGGTGATGGGCGACGTGCCCCGGGACACGCGCGCCCTGCAGAGCGCCGT
GTCGGATATCGGGCTGCTGTTCTCCCGCAACGGCAGCCTCGCCAACCTGCTGTTCCTGGGCCTCGCCTTCGGGATCTCGG
TGGCGCTGTATTTCGGCCGGCGCAACATCGCCCCCAAGGGCGCCCGCCGGGACGCCACCAAGACCGATCCGTCGCGGCGC
GCCGTGATGCTCGCCGCCTGGCGGGTGATCCTGCTCGGCACGGTGCCGGCCGTGGCCGGGTCCTACGCGGTCTACTACGC
CCTCGATTCCACCAACCTGCTGCCGTCCCGGCTCCTGCCGGTCGCCGCCGCGATCCTCGGCGGCCTCGCCTTCATCGCCT
TCGTGGAGGCCCTCTGCGACGGGCTGCTCAGCCCCGAGAAGCCGGCCTGGCGGCCCGCCCCGGTGAGCGACGCGGCGGCC
ACGCGCCTGACCCGGCTCGCCGTCGGCATCGCCACGGTGATCACCGTGGTGAAGTCCGTCGAGGCGCTGAACTCTGGGAT
CTCGGCGGCCCTGCCGATCTCCATCGCCACGCGGGGCATCGGCGCGGTCGCCACCGCGCTGATCCTCGCGGTCGGCCTCC
ACCGCTTCGCCGACACCGAGCAGGAGGAGGAGGCCTGCTTCGGCCCCTACGTGCCGACCAAGACCACGACGGGCATCGGC
GGGCCGGCCCGGCTCCTGGGCTGGGCCGCGGTGGCGCTGATCGGGCTCGGCGCGCTGGTCGGCTACGTGGCCTTCGCGGC
CTTCCTGATCGACCAGCTGATCTGGGACGCGGCCGTCCTCGTGCTGCTCTACCTGCTGGTCCAGAGCGTCGACATCTTCG
TCGGCCGCGCGCTCAGCGACGAGACGCGGCTCGCCACCACGCTCCAGGCCAATACCGGCCTGCGCAAGCGCTCGCTCAAC
CAGATCTCGGTCCTGACGACGGGCGCGACCCGGCTCGTCCTGTTCCTGGCGGCCGCGGTCCTCGTGCTGGCGCCCTGGGG
GCTCGATTCCACCGACATCGTCTCGTCGGTCCGGCAGGCCTTCTTCGGCTTCAAGGTCGGCGACGTCACGATCTCGCTCT
CCAGCATCGCCTTCGGGATCGGCATCCTGGTGGTCGGCGTGTTCATCACCCGCGGGGTGCAGCGCTGGCTCGAGAACACC
TACCTGCCGGCCACCGACCTCGACGCCGGCCTGCGCAACTCGATCACCACGGTGGCGGGCTATTGCGGCTTCCTGATCGC
GCTGGCGCTCGCCTTCTCGTATCTCGGCCTGAGCCTGGAGAAGCTCACCATCGTGGCCGGCGCCCTGTCGGTCGGTATCG
GTTTCGGCCTCCAGTCGATCGTCAACAACTTCGTCTCCGGCCTGCTGCTCCTGTGGGAGCGCCCGATCCGGGTCGGCGAC
CAGGTGGTGATCGGCGATTCCGAGGGTATCGTGAAGCGGATCTCGGTCCGCTCGACCGAGATCCAGACCTTCGACCGCTC
GGCCGTGATCGTGCCGAACTCGAACCTGATCTCCGGCGTGGTCAAGAACCGGGTGCGGGGAGACCGGACCGGGCGCGTCA
GCATCACGGTCAGCGTCCTGCGCAACCAGGATCCGGTGCGCGCCGCCGAGATGATCACGGCCTGCGCGCAGGGCCATGCC
GACGTCCTGAAGGACCCGCCGCCCCGGGTGGTGTTCAAGAAGATCGGCGACCCGTTCCTGGAATTCGAGCTCCTCGTCTG
GATCGTGGACGTGAGCCTCGGCCAGAAGGTGGTGACCGACCTGAACTTCTCGGTGTTCGCGACCCTGTCGGAGGCGGGCT
TCATCCCGCCGCTCGGGCCCGGCTCCAGCATCGTCACCGTGCAGGGCCTCGACACGATGCAGAGCGCCATGGGCGAGATC
GCCAGCCGCTTCATCCAGCCGGCCGCGCTCCCCGAGGGTGGCGAATCAGCCCGGCCCCCGCGCGACCGCGGTCTGCGCAG
CGCCGGCGCCTGA

Upstream 100 bases:

>100_bases
GAGCGCCCGGCCGCCAGGAGGCCCGCCCGGTCCCCGATCGCCGCCGATTCGCCGATGCCGGACTCCGGCCGGCCGCCGCG
CGCCCGACGAGAGGTTGCTC

Downstream 100 bases:

>100_bases
CCGGCCGCCGCCGCGGGCGATCCGGACGGTGTTCCCGCCCGCGTCCTTGGCGGCGTAGAGCGCCGCGTCCGCCCGCCCGA
ACAGCCGGGCGGGGCCGCCG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 910; Mature: 910

Protein sequence:

>910_residues
MHRIPARTRKPEGAPRPSRTLALLAALVFATPAALALPAFAQDPAPAAKSADQKPADHKPADTKAADTKAAPEAKPADGK
APDAQATGARPADAKPAPPPAAKAEVPEQFKKVRAKLDAAKAELDAREKQLGHAELTVVDLNAIRDSAVTVTDEIRTLVT
RLDAPLEAARERLAQLGPKPKDTQESADVAAERAEREAAVTRIDETQRLARSLVVQGDQIVDRVSNRRRESFTRDLLQRS
QPLVGPGLWTKVMGDVPRDTRALQSAVSDIGLLFSRNGSLANLLFLGLAFGISVALYFGRRNIAPKGARRDATKTDPSRR
AVMLAAWRVILLGTVPAVAGSYAVYYALDSTNLLPSRLLPVAAAILGGLAFIAFVEALCDGLLSPEKPAWRPAPVSDAAA
TRLTRLAVGIATVITVVKSVEALNSGISAALPISIATRGIGAVATALILAVGLHRFADTEQEEEACFGPYVPTKTTTGIG
GPARLLGWAAVALIGLGALVGYVAFAAFLIDQLIWDAAVLVLLYLLVQSVDIFVGRALSDETRLATTLQANTGLRKRSLN
QISVLTTGATRLVLFLAAAVLVLAPWGLDSTDIVSSVRQAFFGFKVGDVTISLSSIAFGIGILVVGVFITRGVQRWLENT
YLPATDLDAGLRNSITTVAGYCGFLIALALAFSYLGLSLEKLTIVAGALSVGIGFGLQSIVNNFVSGLLLLWERPIRVGD
QVVIGDSEGIVKRISVRSTEIQTFDRSAVIVPNSNLISGVVKNRVRGDRTGRVSITVSVLRNQDPVRAAEMITACAQGHA
DVLKDPPPRVVFKKIGDPFLEFELLVWIVDVSLGQKVVTDLNFSVFATLSEAGFIPPLGPGSSIVTVQGLDTMQSAMGEI
ASRFIQPAALPEGGESARPPRDRGLRSAGA

Sequences:

>Translated_910_residues
MHRIPARTRKPEGAPRPSRTLALLAALVFATPAALALPAFAQDPAPAAKSADQKPADHKPADTKAADTKAAPEAKPADGK
APDAQATGARPADAKPAPPPAAKAEVPEQFKKVRAKLDAAKAELDAREKQLGHAELTVVDLNAIRDSAVTVTDEIRTLVT
RLDAPLEAARERLAQLGPKPKDTQESADVAAERAEREAAVTRIDETQRLARSLVVQGDQIVDRVSNRRRESFTRDLLQRS
QPLVGPGLWTKVMGDVPRDTRALQSAVSDIGLLFSRNGSLANLLFLGLAFGISVALYFGRRNIAPKGARRDATKTDPSRR
AVMLAAWRVILLGTVPAVAGSYAVYYALDSTNLLPSRLLPVAAAILGGLAFIAFVEALCDGLLSPEKPAWRPAPVSDAAA
TRLTRLAVGIATVITVVKSVEALNSGISAALPISIATRGIGAVATALILAVGLHRFADTEQEEEACFGPYVPTKTTTGIG
GPARLLGWAAVALIGLGALVGYVAFAAFLIDQLIWDAAVLVLLYLLVQSVDIFVGRALSDETRLATTLQANTGLRKRSLN
QISVLTTGATRLVLFLAAAVLVLAPWGLDSTDIVSSVRQAFFGFKVGDVTISLSSIAFGIGILVVGVFITRGVQRWLENT
YLPATDLDAGLRNSITTVAGYCGFLIALALAFSYLGLSLEKLTIVAGALSVGIGFGLQSIVNNFVSGLLLLWERPIRVGD
QVVIGDSEGIVKRISVRSTEIQTFDRSAVIVPNSNLISGVVKNRVRGDRTGRVSITVSVLRNQDPVRAAEMITACAQGHA
DVLKDPPPRVVFKKIGDPFLEFELLVWIVDVSLGQKVVTDLNFSVFATLSEAGFIPPLGPGSSIVTVQGLDTMQSAMGEI
ASRFIQPAALPEGGESARPPRDRGLRSAGA
>Mature_910_residues
MHRIPARTRKPEGAPRPSRTLALLAALVFATPAALALPAFAQDPAPAAKSADQKPADHKPADTKAADTKAAPEAKPADGK
APDAQATGARPADAKPAPPPAAKAEVPEQFKKVRAKLDAAKAELDAREKQLGHAELTVVDLNAIRDSAVTVTDEIRTLVT
RLDAPLEAARERLAQLGPKPKDTQESADVAAERAEREAAVTRIDETQRLARSLVVQGDQIVDRVSNRRRESFTRDLLQRS
QPLVGPGLWTKVMGDVPRDTRALQSAVSDIGLLFSRNGSLANLLFLGLAFGISVALYFGRRNIAPKGARRDATKTDPSRR
AVMLAAWRVILLGTVPAVAGSYAVYYALDSTNLLPSRLLPVAAAILGGLAFIAFVEALCDGLLSPEKPAWRPAPVSDAAA
TRLTRLAVGIATVITVVKSVEALNSGISAALPISIATRGIGAVATALILAVGLHRFADTEQEEEACFGPYVPTKTTTGIG
GPARLLGWAAVALIGLGALVGYVAFAAFLIDQLIWDAAVLVLLYLLVQSVDIFVGRALSDETRLATTLQANTGLRKRSLN
QISVLTTGATRLVLFLAAAVLVLAPWGLDSTDIVSSVRQAFFGFKVGDVTISLSSIAFGIGILVVGVFITRGVQRWLENT
YLPATDLDAGLRNSITTVAGYCGFLIALALAFSYLGLSLEKLTIVAGALSVGIGFGLQSIVNNFVSGLLLLWERPIRVGD
QVVIGDSEGIVKRISVRSTEIQTFDRSAVIVPNSNLISGVVKNRVRGDRTGRVSITVSVLRNQDPVRAAEMITACAQGHA
DVLKDPPPRVVFKKIGDPFLEFELLVWIVDVSLGQKVVTDLNFSVFATLSEAGFIPPLGPGSSIVTVQGLDTMQSAMGEI
ASRFIQPAALPEGGESARPPRDRGLRSAGA

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=299, Percent_Identity=31.7725752508361, Blast_Score=142, Evalue=1e-34,
Organism=Escherichia coli, GI2367355, Length=317, Percent_Identity=29.6529968454259, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1789291, Length=213, Percent_Identity=25.3521126760563, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 96229; Mature: 96229

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRIPARTRKPEGAPRPSRTLALLAALVFATPAALALPAFAQDPAPAAKSADQKPADHKP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHCCHHCCCCCCCCCCCCCCCCCCCC
ADTKAADTKAAPEAKPADGKAPDAQATGARPADAKPAPPPAAKAEVPEQFKKVRAKLDAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KAELDAREKQLGHAELTVVDLNAIRDSAVTVTDEIRTLVTRLDAPLEAARERLAQLGPKP
HHHHHHHHHHCCCCEEEEEEHHHHCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
KDTQESADVAAERAEREAAVTRIDETQRLARSLVVQGDQIVDRVSNRRRESFTRDLLQRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
QPLVGPGLWTKVMGDVPRDTRALQSAVSDIGLLFSRNGSLANLLFLGLAFGISVALYFGR
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
RNIAPKGARRDATKTDPSRRAVMLAAWRVILLGTVPAVAGSYAVYYALDSTNLLPSRLLP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHH
VAAAILGGLAFIAFVEALCDGLLSPEKPAWRPAPVSDAAATRLTRLAVGIATVITVVKSV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EALNSGISAALPISIATRGIGAVATALILAVGLHRFADTEQEEEACFGPYVPTKTTTGIG
HHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCC
GPARLLGWAAVALIGLGALVGYVAFAAFLIDQLIWDAAVLVLLYLLVQSVDIFVGRALSD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ETRLATTLQANTGLRKRSLNQISVLTTGATRLVLFLAAAVLVLAPWGLDSTDIVSSVRQA
CHHHHHHHHHCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FFGFKVGDVTISLSSIAFGIGILVVGVFITRGVQRWLENTYLPATDLDAGLRNSITTVAG
HHCCEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
YCGFLIALALAFSYLGLSLEKLTIVAGALSVGIGFGLQSIVNNFVSGLLLLWERPIRVGD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QVVIGDSEGIVKRISVRSTEIQTFDRSAVIVPNSNLISGVVKNRVRGDRTGRVSITVSVL
EEEEECCCCHHHHHHHCHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEEEEEE
RNQDPVRAAEMITACAQGHADVLKDPPPRVVFKKIGDPFLEFELLVWIVDVSLGQKVVTD
CCCCCHHHHHHHHHHHCCCHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHH
LNFSVFATLSEAGFIPPLGPGSSIVTVQGLDTMQSAMGEIASRFIQPAALPEGGESARPP
CCHHHEEEHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
RDRGLRSAGA
HHHCCCCCCC
>Mature Secondary Structure
MHRIPARTRKPEGAPRPSRTLALLAALVFATPAALALPAFAQDPAPAAKSADQKPADHKP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHCCHHCCCCCCCCCCCCCCCCCCCC
ADTKAADTKAAPEAKPADGKAPDAQATGARPADAKPAPPPAAKAEVPEQFKKVRAKLDAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KAELDAREKQLGHAELTVVDLNAIRDSAVTVTDEIRTLVTRLDAPLEAARERLAQLGPKP
HHHHHHHHHHCCCCEEEEEEHHHHCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
KDTQESADVAAERAEREAAVTRIDETQRLARSLVVQGDQIVDRVSNRRRESFTRDLLQRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
QPLVGPGLWTKVMGDVPRDTRALQSAVSDIGLLFSRNGSLANLLFLGLAFGISVALYFGR
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
RNIAPKGARRDATKTDPSRRAVMLAAWRVILLGTVPAVAGSYAVYYALDSTNLLPSRLLP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHH
VAAAILGGLAFIAFVEALCDGLLSPEKPAWRPAPVSDAAATRLTRLAVGIATVITVVKSV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EALNSGISAALPISIATRGIGAVATALILAVGLHRFADTEQEEEACFGPYVPTKTTTGIG
HHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCC
GPARLLGWAAVALIGLGALVGYVAFAAFLIDQLIWDAAVLVLLYLLVQSVDIFVGRALSD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ETRLATTLQANTGLRKRSLNQISVLTTGATRLVLFLAAAVLVLAPWGLDSTDIVSSVRQA
CHHHHHHHHHCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FFGFKVGDVTISLSSIAFGIGILVVGVFITRGVQRWLENTYLPATDLDAGLRNSITTVAG
HHCCEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
YCGFLIALALAFSYLGLSLEKLTIVAGALSVGIGFGLQSIVNNFVSGLLLLWERPIRVGD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QVVIGDSEGIVKRISVRSTEIQTFDRSAVIVPNSNLISGVVKNRVRGDRTGRVSITVSVL
EEEEECCCCHHHHHHHCHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEEEEEE
RNQDPVRAAEMITACAQGHADVLKDPPPRVVFKKIGDPFLEFELLVWIVDVSLGQKVVTD
CCCCCHHHHHHHHHHHCCCHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHH
LNFSVFATLSEAGFIPPLGPGSSIVTVQGLDTMQSAMGEIASRFIQPAALPEGGESARPP
CCHHHEEEHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
RDRGLRSAGA
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]