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The map label for this gene is hisS [H]

Identifier: 170750680

GI number: 170750680

Start: 4541411

End: 4542958

Strand: Direct

Name: hisS [H]

Synonym: Mrad2831_4290

Alternate gene names: 170750680

Gene position: 4541411-4542958 (Clockwise)

Preceding gene: 170750679

Following gene: 170750681

Centisome position: 74.72

GC content: 71.71

Gene sequence:

>1548_bases
ATGTCCAAGCCCGACACCCCGAAGGCCGAGAAGGCCGACACCCTGAAGCCCCGCCTGCCGCGCGGCTTCGTCGACCGCCG
CGCCGGCGAGATCGCCGCGCAGGGCCGGATGCTGGAGACGATCCGCAAGAGCTTCGAGCTCTACGGGTTCGAGGCCCTGG
AGACGCCCTTCGTCGAGTACACCGAGGCCCTGGGCAAGTTCCTGCCCGACCTCGACCGCCCGAACGCCGGCGTGTTCTCG
TTCCAGGACGACGACGAGGCGTGGCTGTCGCTCCGCTACGACCTGACGGCGCCACTGGCCCGCTTCGTGGCCGAGAACTA
CGACGCCCTGCCCAAGCCCTATCGGAGCTACCGGGCCGGCTACGTCTTCCGCAACGAGAAGCCGGGGCCGGGCCGCTTCC
GCCAGTTCATGCAGTTCGACGCCGACATCGTCGGCGCCGGCAGCGTCGCGGCCGATGCCGAGACCTGCATGCTGATGGCC
GACACGCTGAACCGGCTCGGGCTCGGCGGTCAGTACGTGGTCAAGGTCAACAACCGGAAGGTGCTCGACGGCGTCATGGA
GGCGATCGGGCTCGCCGGTCCCGACAAGGCCGGCCAGCGCCTCACCGTGCTGCGGGCGATCGACAAGCTCGACCGGCTCG
GCGTCGACGGGGTGCGCGACCTGCTCGGGTCCGGCCGCAAGGACGAGAGCGGCGACTTCACCAAGGGCGCGGGCCTCGAC
GCGGCGGCGATCGGCCGGATCCTCGCCTACGTGTCGTTCCAGGCGGATGCCGGCGACGGCGCCGACAAGGTGGCGTTCTG
GGAGAAGTTCTTCGCCGGCTGGCACGACGCGGTCGGCGACTCGGAGACCGGCCGGGAGGGCATCGCCGAGCTGCACGCGA
TCATGCGCCTCTGCGCCGCCGCCGGCTACGGCCACGACGTGATCCGGGCCGATCCGAGCGTGGTGCGCGGGCTCGAATAC
TACACCGGGCCGGTCTACGAGGCCGAGCTGACGTTTCCCGTCACCAACGAGGACGGCCAGACCGTGCGCTTCGGCTCGGT
CGCGGGCGGCGGGCGCTACGACGGCCTCGTCGGGCGGTTCCGGGCCGAGCCGGTGCCGGCCACCGGCTTCTCGATCGGCG
TCTCGCGGCTGTTCTCGGCGCTCCAGCTCGTGGGCAGCCCCCTGATCGCCGGTGCCGAGGCCCCCGGGCCGGTGGTGGTG
CTGGTCCTCGACCGGGAGAACATGGCCGATTACCAGCGGCTCGTCGCGCTCCTGCGCGCCGACGGCATCCGGGCCGAGCT
CTACCTCGGTGCCGCCGGCATGAAGGCGCAGATGAAGTACGCCGACCGCCGCCGCGCGCCCGTGGCGGTGATCCAGGGCT
CGAACGAGCGCCTGGCCGGCGAGGTCCAGATCAAGGACCTGATCGCGGGCGCCCAGGCGGCCGAGTCCATCGCCAGCAAC
GCCGAGTGGAAGGCGGCGCGGCCCGCGCAGGTCTCGTGTCCCGAGGCCGACCTGGTGGCGCAGGTCCGCGCCGTGCTGAG
CCGGCATTTTCCGTCAACCGAGATCTGA

Upstream 100 bases:

>100_bases
GCATGCTCTAGAGCCCGGCCGCAGGATCTCCGCCCGCCGGAGACGATCGAAGATTCGATGCGGGACCCGGATTCCGGGTC
CGCGAGAAGCGAATGCACCC

Downstream 100 bases:

>100_bases
GACACGCGCGGTTCGAACCGCGCCTCGAACCGCGCGTCGAACGCAGGGGCTCCGGCGGCGTTGTCGTCGGTCGGTGCCGT
GGAGATGTCGGGATGCGCGC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSKPDTPKAEKADTLKPRLPRGFVDRRAGEIAAQGRMLETIRKSFELYGFEALETPFVEYTEALGKFLPDLDRPNAGVFS
FQDDDEAWLSLRYDLTAPLARFVAENYDALPKPYRSYRAGYVFRNEKPGPGRFRQFMQFDADIVGAGSVAADAETCMLMA
DTLNRLGLGGQYVVKVNNRKVLDGVMEAIGLAGPDKAGQRLTVLRAIDKLDRLGVDGVRDLLGSGRKDESGDFTKGAGLD
AAAIGRILAYVSFQADAGDGADKVAFWEKFFAGWHDAVGDSETGREGIAELHAIMRLCAAAGYGHDVIRADPSVVRGLEY
YTGPVYEAELTFPVTNEDGQTVRFGSVAGGGRYDGLVGRFRAEPVPATGFSIGVSRLFSALQLVGSPLIAGAEAPGPVVV
LVLDRENMADYQRLVALLRADGIRAELYLGAAGMKAQMKYADRRRAPVAVIQGSNERLAGEVQIKDLIAGAQAAESIASN
AEWKAARPAQVSCPEADLVAQVRAVLSRHFPSTEI

Sequences:

>Translated_515_residues
MSKPDTPKAEKADTLKPRLPRGFVDRRAGEIAAQGRMLETIRKSFELYGFEALETPFVEYTEALGKFLPDLDRPNAGVFS
FQDDDEAWLSLRYDLTAPLARFVAENYDALPKPYRSYRAGYVFRNEKPGPGRFRQFMQFDADIVGAGSVAADAETCMLMA
DTLNRLGLGGQYVVKVNNRKVLDGVMEAIGLAGPDKAGQRLTVLRAIDKLDRLGVDGVRDLLGSGRKDESGDFTKGAGLD
AAAIGRILAYVSFQADAGDGADKVAFWEKFFAGWHDAVGDSETGREGIAELHAIMRLCAAAGYGHDVIRADPSVVRGLEY
YTGPVYEAELTFPVTNEDGQTVRFGSVAGGGRYDGLVGRFRAEPVPATGFSIGVSRLFSALQLVGSPLIAGAEAPGPVVV
LVLDRENMADYQRLVALLRADGIRAELYLGAAGMKAQMKYADRRRAPVAVIQGSNERLAGEVQIKDLIAGAQAAESIASN
AEWKAARPAQVSCPEADLVAQVRAVLSRHFPSTEI
>Mature_514_residues
SKPDTPKAEKADTLKPRLPRGFVDRRAGEIAAQGRMLETIRKSFELYGFEALETPFVEYTEALGKFLPDLDRPNAGVFSF
QDDDEAWLSLRYDLTAPLARFVAENYDALPKPYRSYRAGYVFRNEKPGPGRFRQFMQFDADIVGAGSVAADAETCMLMAD
TLNRLGLGGQYVVKVNNRKVLDGVMEAIGLAGPDKAGQRLTVLRAIDKLDRLGVDGVRDLLGSGRKDESGDFTKGAGLDA
AAIGRILAYVSFQADAGDGADKVAFWEKFFAGWHDAVGDSETGREGIAELHAIMRLCAAAGYGHDVIRADPSVVRGLEYY
TGPVYEAELTFPVTNEDGQTVRFGSVAGGGRYDGLVGRFRAEPVPATGFSIGVSRLFSALQLVGSPLIAGAEAPGPVVVL
VLDRENMADYQRLVALLRADGIRAELYLGAAGMKAQMKYADRRRAPVAVIQGSNERLAGEVQIKDLIAGAQAAESIASNA
EWKAARPAQVSCPEADLVAQVRAVLSRHFPSTEI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=476, Percent_Identity=30.672268907563, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI6996014, Length=463, Percent_Identity=30.2375809935205, Blast_Score=168, Evalue=9e-42,
Organism=Escherichia coli, GI1788861, Length=474, Percent_Identity=27.8481012658228, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71993686, Length=486, Percent_Identity=29.2181069958848, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI71993693, Length=486, Percent_Identity=29.2181069958848, Blast_Score=174, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6325290, Length=486, Percent_Identity=26.9547325102881, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24643061, Length=479, Percent_Identity=32.3590814196242, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24643059, Length=498, Percent_Identity=31.1244979919679, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 55593; Mature: 55461

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPDTPKAEKADTLKPRLPRGFVDRRAGEIAAQGRMLETIRKSFELYGFEALETPFVEY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHCCCHHHH
TEALGKFLPDLDRPNAGVFSFQDDDEAWLSLRYDLTAPLARFVAENYDALPKPYRSYRAG
HHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECHHHHHHHHHHHCCCCCCCCHHHHCCC
YVFRNEKPGPGRFRQFMQFDADIVGAGSVAADAETCMLMADTLNRLGLGGQYVVKVNNRK
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCH
VLDGVMEAIGLAGPDKAGQRLTVLRAIDKLDRLGVDGVRDLLGSGRKDESGDFTKGAGLD
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCC
AAAIGRILAYVSFQADAGDGADKVAFWEKFFAGWHDAVGDSETGREGIAELHAIMRLCAA
HHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
AGYGHDVIRADPSVVRGLEYYTGPVYEAELTFPVTNEDGQTVRFGSVAGGGRYDGLVGRF
CCCCCHHHCCCHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHH
RAEPVPATGFSIGVSRLFSALQLVGSPLIAGAEAPGPVVVLVLDRENMADYQRLVALLRA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHC
DGIRAELYLGAAGMKAQMKYADRRRAPVAVIQGSNERLAGEVQIKDLIAGAQAAESIASN
CCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCEECCEEHHHHHHHHHHHHHHHCC
AEWKAARPAQVSCPEADLVAQVRAVLSRHFPSTEI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKPDTPKAEKADTLKPRLPRGFVDRRAGEIAAQGRMLETIRKSFELYGFEALETPFVEY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHCCCHHHH
TEALGKFLPDLDRPNAGVFSFQDDDEAWLSLRYDLTAPLARFVAENYDALPKPYRSYRAG
HHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECHHHHHHHHHHHCCCCCCCCHHHHCCC
YVFRNEKPGPGRFRQFMQFDADIVGAGSVAADAETCMLMADTLNRLGLGGQYVVKVNNRK
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCH
VLDGVMEAIGLAGPDKAGQRLTVLRAIDKLDRLGVDGVRDLLGSGRKDESGDFTKGAGLD
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCC
AAAIGRILAYVSFQADAGDGADKVAFWEKFFAGWHDAVGDSETGREGIAELHAIMRLCAA
HHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
AGYGHDVIRADPSVVRGLEYYTGPVYEAELTFPVTNEDGQTVRFGSVAGGGRYDGLVGRF
CCCCCHHHCCCHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHH
RAEPVPATGFSIGVSRLFSALQLVGSPLIAGAEAPGPVVVLVLDRENMADYQRLVALLRA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHC
DGIRAELYLGAAGMKAQMKYADRRRAPVAVIQGSNERLAGEVQIKDLIAGAQAAESIASN
CCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCEECCEEHHHHHHHHHHHHHHHCC
AEWKAARPAQVSCPEADLVAQVRAVLSRHFPSTEI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA