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The map label for this gene is rho [H]

Identifier: 170750678

GI number: 170750678

Start: 4538773

End: 4540059

Strand: Reverse

Name: rho [H]

Synonym: Mrad2831_4288

Alternate gene names: 170750678

Gene position: 4540059-4538773 (Counterclockwise)

Preceding gene: 170750686

Following gene: 170750677

Centisome position: 74.7

GC content: 65.66

Gene sequence:

>1287_bases
ATGCCGGCCGCCCTCGAGGGCGTCCGCGAGGTCAAGCTCCAGGACCTCAAATCGAAGACGCCGACCGACCTGACGGCCTT
CGCCGAGGAGGTCGAGGTCGAGAACGCCTCGACCATGCGCAAGCAGGAGCTGATGTTCGCCATCCTGAAGCAGCTCGCTG
CCAAGGAGGTCGAGATCATCGGGTCGGGCACCGTCGAGGTGCTGCAGGACGGCTTCGGCTTCCTGCGCTCCTCCGACTCG
AACTACCTGCCCGGCCCGGACGACATCTACATCTCGCCGACCCAGATCCGCCGCTTCGGCCTGCGGACCGGCGACACCGT
CGAGGGCCCGATCCGCGGCCCCAAGGACGGCGAGCGCTACTTCGCGCTGCTCAAGGTCAACACGATCAACTTCGAGAACC
CGGAGAAGATCAAGCACAAGGTCCACTTCGACAACCTGACGCCGCTCTTCCCGACGAAGCGGTTCAAGCTCGAGCTGGAC
AACCCGACCAAGAAGGACTTCTCGCCGCGGATCATCGACATCGTGGCGCCGATCGGCAAGGGCCAGCGCGCCCTGATCGT
GGCGCCGCCGCGCACGGGTAAGACCGTGCTGATGCAGAACATCGCCCAGTCGATCACCCTCAACCACCCGGAGTGCTACC
TGATCGTGCTGCTCATCGACGAGCGGCCCGAGGAGGTCACCGACATGCAGCGCTCGGTGAAGGGCGAGGTCATCGCCTCG
ACCTTCGACGAGCCGGCCACCCGCCACGTGCAGGTCGCCGAGATGGTGATCGAGAAGGCCAAGCGCCTCGTGGAGCACGG
GCGCGACGTGGTGATCCTCTTGGACTCCATCACCCGCCTCGGCCGCGCCTACAACACCGTGGTGCCGTCCTCCGGCAAGG
TGCTGACCGGCGGTGTCGACGCCAACGCCCTGCAGCGGCCCAAGCGCTTCTTCGGCGCGGCCCGCAACATCGAGGAGGGC
GGCTCGCTCTCGATCATCGCCACGGCGCTGATCGACACCGGCTCGCGCATGGACGAGGTGATCTTCGAGGAGTTCAAGGG
CACCGGCAACTCCGAGATCATCCTGGACCGCAAGGTCTCCGACAAGCGCATCTTCCCGGCGATCGACATCACCCGCTCGG
GCACCCGCAAGGAGGAGCTGCTGGTCCCGCCGGACTCGCTCAAGAAGACCTACGTGCTGCGCCGCATCCTCAACCCGATG
GGCGTCACCGACGCGATCGAGTTCCTGCTCGACAAGCTGCGCCAGACCAAGAGCAACAGCGACTTCTTCGACTCGATGAA
CACCTGA

Upstream 100 bases:

>100_bases
CCGTCGGTCTCAAGATACCCCCATGACGCCAAATCCCGACGCCCTCGCCGACGACATCGAGACCGCGGAGTCGGCCGATG
TGTCCGCCGATCGCCCCACC

Downstream 100 bases:

>100_bases
GGCCATCCCCGGTCGCGGGCATCACCGCCGTCCTCGCGAGCGGAGCGAAGCGATCCAGGCAGCGCCACGGTGGCGAGCCG
CGCGCGGCCCTGGGTCACGT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MPAALEGVREVKLQDLKSKTPTDLTAFAEEVEVENASTMRKQELMFAILKQLAAKEVEIIGSGTVEVLQDGFGFLRSSDS
NYLPGPDDIYISPTQIRRFGLRTGDTVEGPIRGPKDGERYFALLKVNTINFENPEKIKHKVHFDNLTPLFPTKRFKLELD
NPTKKDFSPRIIDIVAPIGKGQRALIVAPPRTGKTVLMQNIAQSITLNHPECYLIVLLIDERPEEVTDMQRSVKGEVIAS
TFDEPATRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEG
GSLSIIATALIDTGSRMDEVIFEEFKGTGNSEIILDRKVSDKRIFPAIDITRSGTRKEELLVPPDSLKKTYVLRRILNPM
GVTDAIEFLLDKLRQTKSNSDFFDSMNT

Sequences:

>Translated_428_residues
MPAALEGVREVKLQDLKSKTPTDLTAFAEEVEVENASTMRKQELMFAILKQLAAKEVEIIGSGTVEVLQDGFGFLRSSDS
NYLPGPDDIYISPTQIRRFGLRTGDTVEGPIRGPKDGERYFALLKVNTINFENPEKIKHKVHFDNLTPLFPTKRFKLELD
NPTKKDFSPRIIDIVAPIGKGQRALIVAPPRTGKTVLMQNIAQSITLNHPECYLIVLLIDERPEEVTDMQRSVKGEVIAS
TFDEPATRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEG
GSLSIIATALIDTGSRMDEVIFEEFKGTGNSEIILDRKVSDKRIFPAIDITRSGTRKEELLVPPDSLKKTYVLRRILNPM
GVTDAIEFLLDKLRQTKSNSDFFDSMNT
>Mature_427_residues
PAALEGVREVKLQDLKSKTPTDLTAFAEEVEVENASTMRKQELMFAILKQLAAKEVEIIGSGTVEVLQDGFGFLRSSDSN
YLPGPDDIYISPTQIRRFGLRTGDTVEGPIRGPKDGERYFALLKVNTINFENPEKIKHKVHFDNLTPLFPTKRFKLELDN
PTKKDFSPRIIDIVAPIGKGQRALIVAPPRTGKTVLMQNIAQSITLNHPECYLIVLLIDERPEEVTDMQRSVKGEVIAST
FDEPATRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGG
SLSIIATALIDTGSRMDEVIFEEFKGTGNSEIILDRKVSDKRIFPAIDITRSGTRKEELLVPPDSLKKTYVLRRILNPMG
VTDAIEFLLDKLRQTKSNSDFFDSMNT

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=67.3076923076923, Blast_Score=600, Evalue=1e-173,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47734; Mature: 47602

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAALEGVREVKLQDLKSKTPTDLTAFAEEVEVENASTMRKQELMFAILKQLAAKEVEII
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEE
GSGTVEVLQDGFGFLRSSDSNYLPGPDDIYISPTQIRRFGLRTGDTVEGPIRGPKDGERY
CCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCCEE
FALLKVNTINFENPEKIKHKVHFDNLTPLFPTKRFKLELDNPTKKDFSPRIIDIVAPIGK
EEEEEEEEECCCCHHHHHHEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCC
GQRALIVAPPRTGKTVLMQNIAQSITLNHPECYLIVLLIDERPEEVTDMQRSVKGEVIAS
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHH
TFDEPATRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVD
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCC
ANALQRPKRFFGAARNIEEGGSLSIIATALIDTGSRMDEVIFEEFKGTGNSEIILDRKVS
CHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCCEEEEECCCC
DKRIFPAIDITRSGTRKEELLVPPDSLKKTYVLRRILNPMGVTDAIEFLLDKLRQTKSNS
CCCCCCEEEECCCCCCCCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
DFFDSMNT
CHHHCCCC
>Mature Secondary Structure 
PAALEGVREVKLQDLKSKTPTDLTAFAEEVEVENASTMRKQELMFAILKQLAAKEVEII
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEE
GSGTVEVLQDGFGFLRSSDSNYLPGPDDIYISPTQIRRFGLRTGDTVEGPIRGPKDGERY
CCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCCEE
FALLKVNTINFENPEKIKHKVHFDNLTPLFPTKRFKLELDNPTKKDFSPRIIDIVAPIGK
EEEEEEEEECCCCHHHHHHEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCC
GQRALIVAPPRTGKTVLMQNIAQSITLNHPECYLIVLLIDERPEEVTDMQRSVKGEVIAS
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHH
TFDEPATRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVD
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCC
ANALQRPKRFFGAARNIEEGGSLSIIATALIDTGSRMDEVIFEEFKGTGNSEIILDRKVS
CHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCCEEEEECCCC
DKRIFPAIDITRSGTRKEELLVPPDSLKKTYVLRRILNPMGVTDAIEFLLDKLRQTKSNS
CCCCCCEEEECCCCCCCCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
DFFDSMNT
CHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]