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The map label for this gene is prpD1 [H]

Identifier: 170750526

GI number: 170750526

Start: 4389261

End: 4390718

Strand: Direct

Name: prpD1 [H]

Synonym: Mrad2831_4133

Alternate gene names: 170750526

Gene position: 4389261-4390718 (Clockwise)

Preceding gene: 170750525

Following gene: 170750535

Centisome position: 72.22

GC content: 70.1

Gene sequence:

>1458_bases
ATGCGGATCCCGACCGACCCCTCCCGCCGTCAGGTGGCCGCGTGGCTGGCCGCGGCCGCATCCCTTGCCGCCGCACCGGC
GAGGGCCGCCTCCGGTGACGGGCCGCCCCTCGCCGACCGGCTCGCGGCCTACGCCCACGCCCTGCGATTCGAGGATCTCG
ACACGGGCACGGTCGAGACCGCGAAGGTCCATCTGATCGACGCGCTGGGATGCGGGCTCGCCGCCCTCGATGCCGGCCCG
GTGCGGATCTGTCGGGACATTGCGCTCAGCGACGGAGCGGGTGCCGCGACGGTGCTCGGCACGGACCGGCGGAGCAGCCC
GGAACTCGCAGCCTTCGCCAACGGCGTCGCCATCCGCTACGCGGATTTCAACGACGTCTATGTCGGCCGCGAGCCCGGTC
ACCCCAGCGACGCCATCGCCGCCTGCCTCGCCGTCGCCGAGGCCGAGCGTGCGACCGGCCGCGAACTGATCGCTTCGATC
GCCCTCGCCTACGAGATCAATTGCCGCCTGCTCGATGCCGCGGAAGTAACGGGGCGTGGCTGGGACCATCCGGTCTACTG
CCTGCCTGCCTCCGCGCTGGCATCGGGCCGGCTGATGCGTCTCTCCCCGGAAGCCCTGACCCAGGCCGTCAATCTCGCGA
TCAACGGCCATATCGCCATGAACCAGACCCGCGTCCAGACGCTCTCGGACTGGAAGGGCGTGGCCGATGCCGAGGCGACC
CGCGCAGGCGTGTTCGCCGCGCGCCTCGCCCGCGCCGGTCTCACGGGCCCGGCGCCGATCTTCGAAGGCCGCGCCGGATT
CTTCAAGCAGGTCTCGGGCCCGTTCACCGTCGACGTGGCGACGTTCGGCGGTGAGGGGCGCCCCTTCCGGATCTCGCGAT
GCGGGATCAAGCCCTATCCGGCCCAGGTCTATTGCCTGACGGCGATCCCGGCGGCGATCGACGTCGTCACGGAAGCCGGT
GGTGTCGACGCCGTTCAGAGCCTGCGCATCGACACGACCCATCTCGGCTACGTCACCGCCGGCCGCGACCGCGAGAAGTG
GCGCCCGACGACGAAGGACACCGCCGATCACAGCCTGCCCTTTATCACCGCGCGGGCGATGCTCGACGGAGCCATCACCA
ACGACAGCTACGCCCCCGACAGGCTGAGCGATCCGGCCCTGCTCGCCCTCATTGACCGGATCACCGTCGCCGAGGACCCT
GCCCTCACGGCGATGCAGCCGAAAGCGGTGCCGAACCGACTGACCGCGACGCTGAGGGATGGGCGCGAGATCGTGAGGCA
GGTCGACGACGTGCCGGGATTCGTGTCGAGGCCGATGACCCGCGACGACGTCGAACGCAAGTTTCGCATGAACGCGGGCA
AGCGTCTCGATCCGCTCGAGATGCGTCGCGTCCTCGACGCCTTCTGGACGATCGATGAACGACCCGACCTGTCCGGTCTG
CTCGCGCTCCTGTCCTGA

Upstream 100 bases:

>100_bases
GACCTGCTCTCCGACTGGGCCGGCAGCGCCGCTGCCCGCGACAGGATCCTCGTCGACAACCCGGCGACGCTCTACGGCTT
CGCCTGAACGGGAGGGCGCG

Downstream 100 bases:

>100_bases
GCAACCCGCAGAAGCGCGTCCCTTCCGCGTTCGGCCGGATCGGCGGGCTGCTTCGAGCCCGTTCGGCCGCGTCACGCGAC
GTGGAGGATCGCTGAAGCAG

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MRIPTDPSRRQVAAWLAAAASLAAAPARAASGDGPPLADRLAAYAHALRFEDLDTGTVETAKVHLIDALGCGLAALDAGP
VRICRDIALSDGAGAATVLGTDRRSSPELAAFANGVAIRYADFNDVYVGREPGHPSDAIAACLAVAEAERATGRELIASI
ALAYEINCRLLDAAEVTGRGWDHPVYCLPASALASGRLMRLSPEALTQAVNLAINGHIAMNQTRVQTLSDWKGVADAEAT
RAGVFAARLARAGLTGPAPIFEGRAGFFKQVSGPFTVDVATFGGEGRPFRISRCGIKPYPAQVYCLTAIPAAIDVVTEAG
GVDAVQSLRIDTTHLGYVTAGRDREKWRPTTKDTADHSLPFITARAMLDGAITNDSYAPDRLSDPALLALIDRITVAEDP
ALTAMQPKAVPNRLTATLRDGREIVRQVDDVPGFVSRPMTRDDVERKFRMNAGKRLDPLEMRRVLDAFWTIDERPDLSGL
LALLS

Sequences:

>Translated_485_residues
MRIPTDPSRRQVAAWLAAAASLAAAPARAASGDGPPLADRLAAYAHALRFEDLDTGTVETAKVHLIDALGCGLAALDAGP
VRICRDIALSDGAGAATVLGTDRRSSPELAAFANGVAIRYADFNDVYVGREPGHPSDAIAACLAVAEAERATGRELIASI
ALAYEINCRLLDAAEVTGRGWDHPVYCLPASALASGRLMRLSPEALTQAVNLAINGHIAMNQTRVQTLSDWKGVADAEAT
RAGVFAARLARAGLTGPAPIFEGRAGFFKQVSGPFTVDVATFGGEGRPFRISRCGIKPYPAQVYCLTAIPAAIDVVTEAG
GVDAVQSLRIDTTHLGYVTAGRDREKWRPTTKDTADHSLPFITARAMLDGAITNDSYAPDRLSDPALLALIDRITVAEDP
ALTAMQPKAVPNRLTATLRDGREIVRQVDDVPGFVSRPMTRDDVERKFRMNAGKRLDPLEMRRVLDAFWTIDERPDLSGL
LALLS
>Mature_485_residues
MRIPTDPSRRQVAAWLAAAASLAAAPARAASGDGPPLADRLAAYAHALRFEDLDTGTVETAKVHLIDALGCGLAALDAGP
VRICRDIALSDGAGAATVLGTDRRSSPELAAFANGVAIRYADFNDVYVGREPGHPSDAIAACLAVAEAERATGRELIASI
ALAYEINCRLLDAAEVTGRGWDHPVYCLPASALASGRLMRLSPEALTQAVNLAINGHIAMNQTRVQTLSDWKGVADAEAT
RAGVFAARLARAGLTGPAPIFEGRAGFFKQVSGPFTVDVATFGGEGRPFRISRCGIKPYPAQVYCLTAIPAAIDVVTEAG
GVDAVQSLRIDTTHLGYVTAGRDREKWRPTTKDTADHSLPFITARAMLDGAITNDSYAPDRLSDPALLALIDRITVAEDP
ALTAMQPKAVPNRLTATLRDGREIVRQVDDVPGFVSRPMTRDDVERKFRMNAGKRLDPLEMRRVLDAFWTIDERPDLSGL
LALLS

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Homo sapiens, GI310129732, Length=469, Percent_Identity=24.9466950959488, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI310110533, Length=469, Percent_Identity=24.9466950959488, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI310123058, Length=467, Percent_Identity=25.0535331905782, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI1786528, Length=456, Percent_Identity=26.3157894736842, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325258, Length=483, Percent_Identity=25.879917184265, Blast_Score=98, Evalue=3e-21,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 51623; Mature: 51623

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPTDPSRRQVAAWLAAAASLAAAPARAASGDGPPLADRLAAYAHALRFEDLDTGTVET
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
AKVHLIDALGCGLAALDAGPVRICRDIALSDGAGAATVLGTDRRSSPELAAFANGVAIRY
EEEEEEHHHCCCHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHCCEEEEE
ADFNDVYVGREPGHPSDAIAACLAVAEAERATGRELIASIALAYEINCRLLDAAEVTGRG
ECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECEEEEECHHHCCCC
WDHPVYCLPASALASGRLMRLSPEALTQAVNLAINGHIAMNQTRVQTLSDWKGVADAEAT
CCCCEEEEEHHHHHCCCEEEECHHHHHHHHHEEEECEEEECHHHHHHHHHHCCCCCCHHH
RAGVFAARLARAGLTGPAPIFEGRAGFFKQVSGPFTVDVATFGGEGRPFRISRCGIKPYP
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCCC
AQVYCLTAIPAAIDVVTEAGGVDAVQSLRIDTTHLGYVTAGRDREKWRPTTKDTADHSLP
CEEEEEEHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCC
FITARAMLDGAITNDSYAPDRLSDPALLALIDRITVAEDPALTAMQPKAVPNRLTATLRD
CEEHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
GREIVRQVDDVPGFVSRPMTRDDVERKFRMNAGKRLDPLEMRRVLDAFWTIDERPDLSGL
HHHHHHHHHCCCCHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
LALLS
HHHCC
>Mature Secondary Structure
MRIPTDPSRRQVAAWLAAAASLAAAPARAASGDGPPLADRLAAYAHALRFEDLDTGTVET
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
AKVHLIDALGCGLAALDAGPVRICRDIALSDGAGAATVLGTDRRSSPELAAFANGVAIRY
EEEEEEHHHCCCHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHCCEEEEE
ADFNDVYVGREPGHPSDAIAACLAVAEAERATGRELIASIALAYEINCRLLDAAEVTGRG
ECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECEEEEECHHHCCCC
WDHPVYCLPASALASGRLMRLSPEALTQAVNLAINGHIAMNQTRVQTLSDWKGVADAEAT
CCCCEEEEEHHHHHCCCEEEECHHHHHHHHHEEEECEEEECHHHHHHHHHHCCCCCCHHH
RAGVFAARLARAGLTGPAPIFEGRAGFFKQVSGPFTVDVATFGGEGRPFRISRCGIKPYP
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCCC
AQVYCLTAIPAAIDVVTEAGGVDAVQSLRIDTTHLGYVTAGRDREKWRPTTKDTADHSLP
CEEEEEEHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCC
FITARAMLDGAITNDSYAPDRLSDPALLALIDRITVAEDPALTAMQPKAVPNRLTATLRD
CEEHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
GREIVRQVDDVPGFVSRPMTRDDVERKFRMNAGKRLDPLEMRRVLDAFWTIDERPDLSGL
HHHHHHHHHCCCCHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
LALLS
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626 [H]