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The map label for this gene is pth

Identifier: 170750518

GI number: 170750518

Start: 4379901

End: 4380509

Strand: Reverse

Name: pth

Synonym: Mrad2831_4125

Alternate gene names: 170750518

Gene position: 4380509-4379901 (Counterclockwise)

Preceding gene: 170750520

Following gene: 170750517

Centisome position: 72.07

GC content: 70.44

Gene sequence:

>609_bases
ATGCGGCTGATCGTCGGCCTCGGAAACCCGGGCGCGCGCTACGCGGGCAACCGCCACAATATCGGCTTCCTCGCCGTCGA
CGCGATCGCCCGCCAGCACCGGGCGAGCCCGTTCCGCCACCGCTTCCAGGGCGAGGCCGCCGAGGTGGTGCTGGGCACCG
AGCGGGCGATCCTGCTGAAGCCCTCGACCTTCATGAACGAGTCCGGCCGCGCGGTCTCGGAGGCGCAGCGCTTCTACAAG
ATCCCGCTGGCCGACGTGATCGTGCTGCACGACGAGCTCGACCTCGCCCCCGCCAAGCTCCGGGTGAAGCGCGGCGGCGG
CAATGCCGGCCATAACGGCCTGCGCTCGATCACCGCGCAGTGCGGCAACGACTACCGGAGGGTGCGGCTCGGCATCGGTC
ATCCCGGCGACAAGGCGCTGGTCCATGCCTACGTGCTCAACGATTTCGGCAAGGCCGAGCTGCCCTGGGTCGAGGATCTC
TGCCGGGCGGTGGCCGACCACGCCGCCCTGCTCGCCGCCGGCGAGGACGCGAGCTTCCAGAACAAGGTCCACCTCGCCAT
GGCCGGCCGCGGCTGGGACGACGTGAAGACGCTGGGCGCCAGAAATTGA

Upstream 100 bases:

>100_bases
CGCGGCGCGGGCCGGGAGGCCGGAGACCGGCCCGGCCCGGCCGGGGATGCGTCCCGCGCCGAAGCGTGGCACAGACCGCG
GCCTGAAAGGGATGCAGACC

Downstream 100 bases:

>100_bases
CACGCCCACACGGGGACGGGATATCGGGGCGGGCGAGAGGGTCCGATGAAGGCGGTGCGCGTCGCGCCCGGCTGTCGGCG
GTTCGATTGCCTCCCGGCGG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MRLIVGLGNPGARYAGNRHNIGFLAVDAIARQHRASPFRHRFQGEAAEVVLGTERAILLKPSTFMNESGRAVSEAQRFYK
IPLADVIVLHDELDLAPAKLRVKRGGGNAGHNGLRSITAQCGNDYRRVRLGIGHPGDKALVHAYVLNDFGKAELPWVEDL
CRAVADHAALLAAGEDASFQNKVHLAMAGRGWDDVKTLGARN

Sequences:

>Translated_202_residues
MRLIVGLGNPGARYAGNRHNIGFLAVDAIARQHRASPFRHRFQGEAAEVVLGTERAILLKPSTFMNESGRAVSEAQRFYK
IPLADVIVLHDELDLAPAKLRVKRGGGNAGHNGLRSITAQCGNDYRRVRLGIGHPGDKALVHAYVLNDFGKAELPWVEDL
CRAVADHAALLAAGEDASFQNKVHLAMAGRGWDDVKTLGARN
>Mature_202_residues
MRLIVGLGNPGARYAGNRHNIGFLAVDAIARQHRASPFRHRFQGEAAEVVLGTERAILLKPSTFMNESGRAVSEAQRFYK
IPLADVIVLHDELDLAPAKLRVKRGGGNAGHNGLRSITAQCGNDYRRVRLGIGHPGDKALVHAYVLNDFGKAELPWVEDL
CRAVADHAALLAAGEDASFQNKVHLAMAGRGWDDVKTLGARN

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=164, Percent_Identity=36.5853658536585, Blast_Score=92, Evalue=3e-19,
Organism=Escherichia coli, GI1787455, Length=150, Percent_Identity=49.3333333333333, Blast_Score=118, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321983, Length=165, Percent_Identity=31.5151515151515, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_METRJ (B1M1C0)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001756778.1
- ProteinModelPortal:   B1M1C0
- SMR:   B1M1C0
- GeneID:   6140183
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_4125
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21917; Mature: 21917

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIVGLGNPGARYAGNRHNIGFLAVDAIARQHRASPFRHRFQGEAAEVVLGTERAILLK
CEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCCHHHHHHCCCCEEEEEECCEEEEEC
PSTFMNESGRAVSEAQRFYKIPLADVIVLHDELDLAPAKLRVKRGGGNAGHNGLRSITAQ
CHHHCCCCCCHHHHHHHHHCCCHHHEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHH
CGNDYRRVRLGIGHPGDKALVHAYVLNDFGKAELPWVEDLCRAVADHAALLAAGEDASFQ
HCCCEEEEEEECCCCCCHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCC
NKVHLAMAGRGWDDVKTLGARN
CEEEEEEECCCCHHHHHCCCCC
>Mature Secondary Structure
MRLIVGLGNPGARYAGNRHNIGFLAVDAIARQHRASPFRHRFQGEAAEVVLGTERAILLK
CEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCCHHHHHHCCCCEEEEEECCEEEEEC
PSTFMNESGRAVSEAQRFYKIPLADVIVLHDELDLAPAKLRVKRGGGNAGHNGLRSITAQ
CHHHCCCCCCHHHHHHHHHCCCHHHEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHH
CGNDYRRVRLGIGHPGDKALVHAYVLNDFGKAELPWVEDLCRAVADHAALLAAGEDASFQ
HCCCEEEEEEECCCCCCHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCC
NKVHLAMAGRGWDDVKTLGARN
CEEEEEEECCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA