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The map label for this gene is trmE

Identifier: 170750474

GI number: 170750474

Start: 4333634

End: 4334959

Strand: Reverse

Name: trmE

Synonym: Mrad2831_4081

Alternate gene names: 170750474

Gene position: 4334959-4333634 (Counterclockwise)

Preceding gene: 170750480

Following gene: 170750471

Centisome position: 71.32

GC content: 76.02

Gene sequence:

>1326_bases
ATGAACGAGACCGACACGCTCTTCGCCCCGGCCAGCGGCTTCGGCCGGGCCGCCGTCGCGGTCATCCGCATCAGCGGCCC
GGCCGCGGGCGGCGTGCTCGCCACCCTGGGCGGCCGCCTGCCGACCCCGCGGCGGCTGTCCCTGCGGAGCCTCAGGGATC
CGCGGGACGGCACCGAGCTCGACCGGGCGCTGGTGGCGTGGTTTCCCGGGCCGGACACCTACAGCGGCGAGGATATGGCG
GAGCTGCACCTCCACGGCGGCGCGGCCGTGCGGATGCGCGTCCTCGCGACCCTCGCCCGCCTTCCCGGATGCCGGGCGGC
CGAGCCCGGGGCGTTCACGCGGCGGGCGGTCCTCAACGGCCGGATGGATCTCGCCGAGGCGGAGGCCGTCGCCGACCTGA
TCGACGCCGAGACCGAGGGCCAGCGCCGGCAGGCCCTGCGCCAGCTCGACGGCGCGCTCAGCCGGCAGGTGGCCGCGTGG
CGCGCGGAGGCGATCGACTGCCTCGCCGCCGCGGAGGCCGCGCTCGACTTCGCCGACGAGGGGGACGTGGACGATGCCGG
GCTCGACGCCGCGCTGTTCGACCGCGCCGCGCGCCTGCGGGACGCGGTCGCCGCGACGCTGCGCGACGGCCACCGCGGCG
AGCGCCTGCGCGAAGGATTCACGGTGGTGCTGGCGGGGGCGCCCAACAGCGGGAAGTCGACGCTGCTGAACGCCCTGAGC
CGGCGGGACGTCGCCATCGTGTCGGACAGCCCGGGGACGACCCGGGACGCCATCGAGGTGCGGCTCGACCTCGGCGGCCT
GCCGGTCCTCCTGGTCGACACGGCCGGGCTCCGCGAGACCGCGGAGCCAATCGAGGCGCAGGGCATCGTGCGGACCCGGG
CGCGGATCGACACGGCCGATCTGGTGGTCGCCCTCGTGCCCCCCGGCGGTGCTGTCCCGGATCTCGGGCCGGGATGCCGT
CCGATCCTGATCGTCCGCACGAAGGCCGATCTTTTTGCCGGCTCACAGCCCGCCGGCGACTCGGCCGACGTGACGGTCTC
GGCCCATACCGGCGCCGGCATGGACGACCTGCTCGACGCGATCCAGGCCGCGGCCGAGGACGGTCTCGGCACCGGCGACG
CACTGATCACCCGCGCCCGTCACAGGGCCGCGCTCGAGGCCTGCGTGGCCCATCTCGACCGCGTCTCCGGGAGCGCCGGC
GGTCTGCCGGAGCTGGCCGCGGAGGACCTGCGGCTCGCGGTCCGGGCGCTGGGCGAGGTGGCCGGCCATGTCGGCGTCGA
GGACGTCCTCGACCGGCTGTTCAGCGGCTTCTGCATCGGGAAGTAG

Upstream 100 bases:

>100_bases
GGCCGCGCGCCCGGGCGCGTCACCGCGACGCCGGGATGGTATAGGGCGGCCGCGCGTGTTTCACGTGAAACTCCGCCCCT
CCGCGCGGCCCTGTGGGACC

Downstream 100 bases:

>100_bases
ATCTGTAGCTGGCAACCCATCGGGCGGAAACCGGCCCGGCCGGGCGCCGTCGCCGGCTCCCGCAGCCCTGCGCGGCGGCG
CTACCGGGGAATCCGCAGGA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MNETDTLFAPASGFGRAAVAVIRISGPAAGGVLATLGGRLPTPRRLSLRSLRDPRDGTELDRALVAWFPGPDTYSGEDMA
ELHLHGGAAVRMRVLATLARLPGCRAAEPGAFTRRAVLNGRMDLAEAEAVADLIDAETEGQRRQALRQLDGALSRQVAAW
RAEAIDCLAAAEAALDFADEGDVDDAGLDAALFDRAARLRDAVAATLRDGHRGERLREGFTVVLAGAPNSGKSTLLNALS
RRDVAIVSDSPGTTRDAIEVRLDLGGLPVLLVDTAGLRETAEPIEAQGIVRTRARIDTADLVVALVPPGGAVPDLGPGCR
PILIVRTKADLFAGSQPAGDSADVTVSAHTGAGMDDLLDAIQAAAEDGLGTGDALITRARHRAALEACVAHLDRVSGSAG
GLPELAAEDLRLAVRALGEVAGHVGVEDVLDRLFSGFCIGK

Sequences:

>Translated_441_residues
MNETDTLFAPASGFGRAAVAVIRISGPAAGGVLATLGGRLPTPRRLSLRSLRDPRDGTELDRALVAWFPGPDTYSGEDMA
ELHLHGGAAVRMRVLATLARLPGCRAAEPGAFTRRAVLNGRMDLAEAEAVADLIDAETEGQRRQALRQLDGALSRQVAAW
RAEAIDCLAAAEAALDFADEGDVDDAGLDAALFDRAARLRDAVAATLRDGHRGERLREGFTVVLAGAPNSGKSTLLNALS
RRDVAIVSDSPGTTRDAIEVRLDLGGLPVLLVDTAGLRETAEPIEAQGIVRTRARIDTADLVVALVPPGGAVPDLGPGCR
PILIVRTKADLFAGSQPAGDSADVTVSAHTGAGMDDLLDAIQAAAEDGLGTGDALITRARHRAALEACVAHLDRVSGSAG
GLPELAAEDLRLAVRALGEVAGHVGVEDVLDRLFSGFCIGK
>Mature_441_residues
MNETDTLFAPASGFGRAAVAVIRISGPAAGGVLATLGGRLPTPRRLSLRSLRDPRDGTELDRALVAWFPGPDTYSGEDMA
ELHLHGGAAVRMRVLATLARLPGCRAAEPGAFTRRAVLNGRMDLAEAEAVADLIDAETEGQRRQALRQLDGALSRQVAAW
RAEAIDCLAAAEAALDFADEGDVDDAGLDAALFDRAARLRDAVAATLRDGHRGERLREGFTVVLAGAPNSGKSTLLNALS
RRDVAIVSDSPGTTRDAIEVRLDLGGLPVLLVDTAGLRETAEPIEAQGIVRTRARIDTADLVVALVPPGGAVPDLGPGCR
PILIVRTKADLFAGSQPAGDSADVTVSAHTGAGMDDLLDAIQAAAEDGLGTGDALITRARHRAALEACVAHLDRVSGSAG
GLPELAAEDLRLAVRALGEVAGHVGVEDVLDRLFSGFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=461, Percent_Identity=44.6854663774403, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI193082993, Length=444, Percent_Identity=45.945945945946, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI193082988, Length=492, Percent_Identity=41.6666666666667, Blast_Score=314, Evalue=9e-86,
Organism=Escherichia coli, GI2367268, Length=462, Percent_Identity=40.4761904761905, Blast_Score=285, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17507259, Length=450, Percent_Identity=31.5555555555556, Blast_Score=197, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6323665, Length=479, Percent_Identity=32.9853862212944, Blast_Score=236, Evalue=6e-63,
Organism=Drosophila melanogaster, GI45550813, Length=472, Percent_Identity=36.0169491525424, Blast_Score=271, Evalue=8e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_METRJ (B1M0E0)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001756734.1
- ProteinModelPortal:   B1M0E0
- SMR:   B1M0E0
- GeneID:   6140139
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_4081
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 45736; Mature: 45736

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 81-81 BINDING 121-121 BINDING 441-441

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETDTLFAPASGFGRAAVAVIRISGPAAGGVLATLGGRLPTPRRLSLRSLRDPRDGTEL
CCCCCCEEECCCCCCCEEEEEEEECCCCCCCHHHHHCCCCCCCCHHHHHHCCCCCCCHHH
DRALVAWFPGPDTYSGEDMAELHLHGGAAVRMRVLATLARLPGCRAAEPGAFTRRAVLNG
HHEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
RMDLAEAEAVADLIDAETEGQRRQALRQLDGALSRQVAAWRAEAIDCLAAAEAALDFADE
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GDVDDAGLDAALFDRAARLRDAVAATLRDGHRGERLREGFTVVLAGAPNSGKSTLLNALS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCHHHHHHHHH
RRDVAIVSDSPGTTRDAIEVRLDLGGLPVLLVDTAGLRETAEPIEAQGIVRTRARIDTAD
CCCEEEEECCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHCCHHHCCHHHHHHHCCCCC
LVVALVPPGGAVPDLGPGCRPILIVRTKADLFAGSQPAGDSADVTVSAHTGAGMDDLLDA
EEEEEECCCCCCCCCCCCCCEEEEEEECHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHH
IQAAAEDGLGTGDALITRARHRAALEACVAHLDRVSGSAGGLPELAAEDLRLAVRALGEV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
AGHVGVEDVLDRLFSGFCIGK
HHCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNETDTLFAPASGFGRAAVAVIRISGPAAGGVLATLGGRLPTPRRLSLRSLRDPRDGTEL
CCCCCCEEECCCCCCCEEEEEEEECCCCCCCHHHHHCCCCCCCCHHHHHHCCCCCCCHHH
DRALVAWFPGPDTYSGEDMAELHLHGGAAVRMRVLATLARLPGCRAAEPGAFTRRAVLNG
HHEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
RMDLAEAEAVADLIDAETEGQRRQALRQLDGALSRQVAAWRAEAIDCLAAAEAALDFADE
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GDVDDAGLDAALFDRAARLRDAVAATLRDGHRGERLREGFTVVLAGAPNSGKSTLLNALS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCHHHHHHHHH
RRDVAIVSDSPGTTRDAIEVRLDLGGLPVLLVDTAGLRETAEPIEAQGIVRTRARIDTAD
CCCEEEEECCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHCCHHHCCHHHHHHHCCCCC
LVVALVPPGGAVPDLGPGCRPILIVRTKADLFAGSQPAGDSADVTVSAHTGAGMDDLLDA
EEEEEECCCCCCCCCCCCCCEEEEEEECHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHH
IQAAAEDGLGTGDALITRARHRAALEACVAHLDRVSGSAGGLPELAAEDLRLAVRALGEV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
AGHVGVEDVLDRLFSGFCIGK
HHCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA