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The map label for this gene is rutD [H]

Identifier: 170750451

GI number: 170750451

Start: 4303582

End: 4304541

Strand: Reverse

Name: rutD [H]

Synonym: Mrad2831_4056

Alternate gene names: 170750451

Gene position: 4304541-4303582 (Counterclockwise)

Preceding gene: 170750452

Following gene: 170750449

Centisome position: 70.82

GC content: 75.62

Gene sequence:

>960_bases
ATGAGCGCGCCGGACGCGTCGGGGCACCTGCCGGCCAACGGCGTCACCCTGGCCTATGACTGCTTCGGCGACGCGGCCGC
CGAGACGATCCTCCTGATCGCCGGTCTCGGGACGCAGATGATCCGCTGGACCGAGGCCTTCTGCCGGGCGCTGGCGGAGC
GCGGCTTCCGGGTCGTGCGGTTCGACAACCGCGATTCGGGGCGCTCGACCGCCTTCACCGCGCACGGCGCCACGGACTTC
GCGGCCTTGTCGGCGGCGCTGGCCGAAGGTCGGCGGCCGGACCTCGCCTACACGCTGGCCGACATGGCGCAGGACGCGGT
CGGGCTGCTCGATGCCCTGTCGGTCCGCCGCGCCCACGTCGTCGGCCGCTCCATGGGCGGCATGATCGCCCAGATCCTGG
CGAGCGAGCATCCCGCGCGGGTTGGATCCCTCACCGCGATCATGTCGAGCACGGGCAACCCGGGCCTGCCCCCGGCCGCG
CCCGACGCGATGGCCCTGATGATGCGGCCCGCCCCCGACCCGTCGCTGGACGAGGCGGGCTTCGTCGCGCAGGCCGTCGC
CGTCGCCCGGCGGCTGGCCGGCCGGTCCGACCCTCTGGACGAGGCGGCGTACCGGGCACTCATCCGCGCGGAGCTGCGCC
GCGGCTACGTCCCGGGCGGCTTCGGACGGCAGCTCGCCGCGCTGGTCCTCGCGGGCGACCGACGCGCCCGGTTGGCGGCG
ATCCGCGCGCCGACCCTCGTGGTGCACGGGACCGAGGACCCCCTGTTCCCGCTCGCCTGCGGGCAGGACATTGCCGACTC
GATCCCCGGCGCGGCCTGGCTGCCCATCCAGGGGATGGGACACGACCTGCCGCCGTCGCTGCACGGTCCCATCGCCGAAG
CGATCGCGCGAACCGCGCGCCGCGCGACCCCGTCGGTGCCGGGCGAACAGCCGGCCATCGATCCGGGGCGCGGCGCGTGA

Upstream 100 bases:

>100_bases
TCGTCGAGCGGCTGGACGAGGAGGCGAGCGGCTACGTCAGGGACCGGGCGATGAGGGAGCACCTGACCCGATGGGCGGAC
CGCCTGGGGGCGGTCCGGAT

Downstream 100 bases:

>100_bases
CGGCTCGGCCGCGGCGGTCCCGGTCGCATCAGCCGCCGCTGCGCGAGGCGCGGTAGTCGCGCGGCGTCAGGCCGTAGGCC
TCCCGGAAGGCACGGCTGAG

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSAPDASGHLPANGVTLAYDCFGDAAAETILLIAGLGTQMIRWTEAFCRALAERGFRVVRFDNRDSGRSTAFTAHGATDF
AALSAALAEGRRPDLAYTLADMAQDAVGLLDALSVRRAHVVGRSMGGMIAQILASEHPARVGSLTAIMSSTGNPGLPPAA
PDAMALMMRPAPDPSLDEAGFVAQAVAVARRLAGRSDPLDEAAYRALIRAELRRGYVPGGFGRQLAALVLAGDRRARLAA
IRAPTLVVHGTEDPLFPLACGQDIADSIPGAAWLPIQGMGHDLPPSLHGPIAEAIARTARRATPSVPGEQPAIDPGRGA

Sequences:

>Translated_319_residues
MSAPDASGHLPANGVTLAYDCFGDAAAETILLIAGLGTQMIRWTEAFCRALAERGFRVVRFDNRDSGRSTAFTAHGATDF
AALSAALAEGRRPDLAYTLADMAQDAVGLLDALSVRRAHVVGRSMGGMIAQILASEHPARVGSLTAIMSSTGNPGLPPAA
PDAMALMMRPAPDPSLDEAGFVAQAVAVARRLAGRSDPLDEAAYRALIRAELRRGYVPGGFGRQLAALVLAGDRRARLAA
IRAPTLVVHGTEDPLFPLACGQDIADSIPGAAWLPIQGMGHDLPPSLHGPIAEAIARTARRATPSVPGEQPAIDPGRGA
>Mature_318_residues
SAPDASGHLPANGVTLAYDCFGDAAAETILLIAGLGTQMIRWTEAFCRALAERGFRVVRFDNRDSGRSTAFTAHGATDFA
ALSAALAEGRRPDLAYTLADMAQDAVGLLDALSVRRAHVVGRSMGGMIAQILASEHPARVGSLTAIMSSTGNPGLPPAAP
DAMALMMRPAPDPSLDEAGFVAQAVAVARRLAGRSDPLDEAAYRALIRAELRRGYVPGGFGRQLAALVLAGDRRARLAAI
RAPTLVVHGTEDPLFPLACGQDIADSIPGAAWLPIQGMGHDLPPSLHGPIAEAIARTARRATPSVPGEQPAIDPGRGA

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 33100; Mature: 32969

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPDASGHLPANGVTLAYDCFGDAAAETILLIAGLGTQMIRWTEAFCRALAERGFRVVR
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEE
FDNRDSGRSTAFTAHGATDFAALSAALAEGRRPDLAYTLADMAQDAVGLLDALSVRRAHV
ECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VGRSMGGMIAQILASEHPARVGSLTAIMSSTGNPGLPPAAPDAMALMMRPAPDPSLDEAG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCCCCCCCHHH
FVAQAVAVARRLAGRSDPLDEAAYRALIRAELRRGYVPGGFGRQLAALVLAGDRRARLAA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHE
IRAPTLVVHGTEDPLFPLACGQDIADSIPGAAWLPIQGMGHDLPPSLHGPIAEAIARTAR
EECCEEEEECCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH
RATPSVPGEQPAIDPGRGA
HCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAPDASGHLPANGVTLAYDCFGDAAAETILLIAGLGTQMIRWTEAFCRALAERGFRVVR
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEE
FDNRDSGRSTAFTAHGATDFAALSAALAEGRRPDLAYTLADMAQDAVGLLDALSVRRAHV
ECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VGRSMGGMIAQILASEHPARVGSLTAIMSSTGNPGLPPAAPDAMALMMRPAPDPSLDEAG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCCCCCCCHHH
FVAQAVAVARRLAGRSDPLDEAAYRALIRAELRRGYVPGGFGRQLAALVLAGDRRARLAA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHE
IRAPTLVVHGTEDPLFPLACGQDIADSIPGAAWLPIQGMGHDLPPSLHGPIAEAIARTAR
EECCEEEEECCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH
RATPSVPGEQPAIDPGRGA
HCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA