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The map label for this gene is ansA [H]

Identifier: 170750424

GI number: 170750424

Start: 4272741

End: 4273757

Strand: Reverse

Name: ansA [H]

Synonym: Mrad2831_4029

Alternate gene names: 170750424

Gene position: 4273757-4272741 (Counterclockwise)

Preceding gene: 170750425

Following gene: 170750423

Centisome position: 70.32

GC content: 75.12

Gene sequence:

>1017_bases
ATGGCGGCAGGATCGGGGACCGGATTGCCCAAGGTCGCGTTCATCGGCACGGGCGGGACGATCTCGTCCCTCGGCCGCGA
CGCCCTCGACATCCTCGACTACACCGCCACCGACCGGCGCCTCGAGGCCGGCGCGATCCTGGACGCCGTCCCGGAACTCG
CGACGGTCGCCACGGTGGTGCCGGTGCCGTTCCGGGCGGTCCCGAGCCCGGCGATCGGATTCGCCGAGTGGCGCGACCTC
GTCCGCCTGTGCGAGCGCCTCGCGGCCGAGCACCCGGATCTCGCCGGCATCGTCATCGGGCACGGCACCGCCACCCTGGA
GGAGACCGCCTACGCCCTCAGCCTGACCCTGACGGTGGATCTGCCGGTGGTGCTGGTCGGCTCGCAGCGGCCGATGAGCG
CCCTCTCCACGGATGCCAAGCTGAACCTCGTGGCGGCCGTGCGCACGGCCGCGGCGCCGGCCTCCCGCGGGCGCGGCGTG
CTGGTGGTGCTCAACGACGAGATCCAGGCGGCCCGCGAGGTGACCAAGACCTCGGTGGCGCGGCTCCAGACCTTCCGGAC
CCCGGATTTCGGCGTGCTCGGCCACGTCGACGGCGAGTCCGTCCGCTACTACCGCCGCTCCGAGCGGGCGCACGCGCCGG
GCACGCCGTTCGACATCCGGACGCTGTCGGCGCTGCCGCGGGTCGACGTCGCCTACGGATACGCCGACGCCGACGGCACC
GCGGTCCGGGCCTTCCTGGCCGCCGGCGCGCGCGGCCTCGTCGCGGCCGGTCTCGCCCCCGGCATGACCCCGCCCGCCGA
GGCCGAGGCCCTGGAGGCGGCGGCCGCCGCCGGGATCGTGGTGGTGATGTCGACCCGGGCCGGCAGCGGGGTGGTCCCGC
TCACCACCCGGCTGCGCAACCGCGGCATCCTCAGCGCCGACAACCTCACCCCGCAGAAGGCCCGGATCCTGCTCGCCCTG
GCGCTGACCGTCACGGCGGAGCCCGAGGCGGTGGCGCGGCTGTTCGCGACCTACTAG

Upstream 100 bases:

>100_bases
CGGGCCTCCCGCCCGGCGCATCGCCCGCCTGCGCGCGGCCGGGGTCGACCGCGGCCGCGCGACGCGGCATCACAGCGTGG
ACAGGATCGGGAGAGACGGC

Downstream 100 bases:

>100_bases
GCGCCGCGCCCGCCGGGCCCCGGACGCTCCCCGACGCGCGTGGGCTGCCACGCGCGAGCCCGTCGCGCGCCTGCGGGACG
CGCCGGCGCGGCGGGCCGCA

Product: asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MAAGSGTGLPKVAFIGTGGTISSLGRDALDILDYTATDRRLEAGAILDAVPELATVATVVPVPFRAVPSPAIGFAEWRDL
VRLCERLAAEHPDLAGIVIGHGTATLEETAYALSLTLTVDLPVVLVGSQRPMSALSTDAKLNLVAAVRTAAAPASRGRGV
LVVLNDEIQAAREVTKTSVARLQTFRTPDFGVLGHVDGESVRYYRRSERAHAPGTPFDIRTLSALPRVDVAYGYADADGT
AVRAFLAAGARGLVAAGLAPGMTPPAEAEALEAAAAAGIVVVMSTRAGSGVVPLTTRLRNRGILSADNLTPQKARILLAL
ALTVTAEPEAVARLFATY

Sequences:

>Translated_338_residues
MAAGSGTGLPKVAFIGTGGTISSLGRDALDILDYTATDRRLEAGAILDAVPELATVATVVPVPFRAVPSPAIGFAEWRDL
VRLCERLAAEHPDLAGIVIGHGTATLEETAYALSLTLTVDLPVVLVGSQRPMSALSTDAKLNLVAAVRTAAAPASRGRGV
LVVLNDEIQAAREVTKTSVARLQTFRTPDFGVLGHVDGESVRYYRRSERAHAPGTPFDIRTLSALPRVDVAYGYADADGT
AVRAFLAAGARGLVAAGLAPGMTPPAEAEALEAAAAAGIVVVMSTRAGSGVVPLTTRLRNRGILSADNLTPQKARILLAL
ALTVTAEPEAVARLFATY
>Mature_337_residues
AAGSGTGLPKVAFIGTGGTISSLGRDALDILDYTATDRRLEAGAILDAVPELATVATVVPVPFRAVPSPAIGFAEWRDLV
RLCERLAAEHPDLAGIVIGHGTATLEETAYALSLTLTVDLPVVLVGSQRPMSALSTDAKLNLVAAVRTAAAPASRGRGVL
VVLNDEIQAAREVTKTSVARLQTFRTPDFGVLGHVDGESVRYYRRSERAHAPGTPFDIRTLSALPRVDVAYGYADADGTA
VRAFLAAGARGLVAAGLAPGMTPPAEAEALEAAAAAGIVVVMSTRAGSGVVPLTTRLRNRGILSADNLTPQKARILLALA
LTVTAEPEAVARLFATY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=295, Percent_Identity=37.6271186440678, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1788065, Length=222, Percent_Identity=29.2792792792793, Blast_Score=71, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=327, Percent_Identity=33.9449541284404, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323187, Length=327, Percent_Identity=33.9449541284404, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323186, Length=327, Percent_Identity=33.9449541284404, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323184, Length=327, Percent_Identity=33.9449541284404, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320527, Length=336, Percent_Identity=31.547619047619, Blast_Score=142, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34978; Mature: 34846

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGSGTGLPKVAFIGTGGTISSLGRDALDILDYTATDRRLEAGAILDAVPELATVATVV
CCCCCCCCCCEEEEEECCCCHHHHCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHC
PVPFRAVPSPAIGFAEWRDLVRLCERLAAEHPDLAGIVIGHGTATLEETAYALSLTLTVD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCEEEEEEEEEEC
LPVVLVGSQRPMSALSTDAKLNLVAAVRTAAAPASRGRGVLVVLNDEIQAAREVTKTSVA
CEEEEECCCCCHHHHCCCCCEEEEEHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHH
RLQTFRTPDFGVLGHVDGESVRYYRRSERAHAPGTPFDIRTLSALPRVDVAYGYADADGT
HHHHCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCH
AVRAFLAAGARGLVAAGLAPGMTPPAEAEALEAAAAAGIVVVMSTRAGSGVVPLTTRLRN
HHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHC
RGILSADNLTPQKARILLALALTVTAEPEAVARLFATY
CCCEECCCCCHHHHHEEEEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure 
AAGSGTGLPKVAFIGTGGTISSLGRDALDILDYTATDRRLEAGAILDAVPELATVATVV
CCCCCCCCCEEEEEECCCCHHHHCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHC
PVPFRAVPSPAIGFAEWRDLVRLCERLAAEHPDLAGIVIGHGTATLEETAYALSLTLTVD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCEEEEEEEEEEC
LPVVLVGSQRPMSALSTDAKLNLVAAVRTAAAPASRGRGVLVVLNDEIQAAREVTKTSVA
CEEEEECCCCCHHHHCCCCCEEEEEHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHH
RLQTFRTPDFGVLGHVDGESVRYYRRSERAHAPGTPFDIRTLSALPRVDVAYGYADADGT
HHHHCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCH
AVRAFLAAGARGLVAAGLAPGMTPPAEAEALEAAAAAGIVVVMSTRAGSGVVPLTTRLRN
HHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHC
RGILSADNLTPQKARILLALALTVTAEPEAVARLFATY
CCCEECCCCCHHHHHEEEEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8898907; 14500908 [H]