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The map label for this gene is cysK [H]

Identifier: 170750412

GI number: 170750412

Start: 4262266

End: 4263231

Strand: Reverse

Name: cysK [H]

Synonym: Mrad2831_4017

Alternate gene names: 170750412

Gene position: 4263231-4262266 (Counterclockwise)

Preceding gene: 170750414

Following gene: 170750411

Centisome position: 70.14

GC content: 66.46

Gene sequence:

>966_bases
ATGCGCCCCACATCGCTGTCCCCATCTCCTTCGACTGCGCGGATCAAGCCTTCCGCCCTCGACCTGATCGGCCATACGCC
ACTGATCGCCCTCGACCGCATCCACCGTGGCCCCGGCCGCATCCTGGCGAAGGCCGAGTTCATGCAGCCCGGTGGGAGCG
TCAAGGATCGGGCGGCCCGTGCCATCGTCCTTGCCGCGCGTGAGGACGGGCGTTTGGCCCCCGGTGCGCCCGTTGTGGAG
ATGACCAGCGGCAACATGGGAGCCGGTCTCGCGGTGGCCTGCGCTGCACTGGGACATCCCCTCGTGGTCACGATGTCCGC
CGGGAACAGCCCCCAGCGTGCCCGGATGCTCGAAGCGCTCGGGGCCGAAGTTATTCTGGTGCCGCAGATCGACGGAGCAG
CTGGGCAAGTCACCGGGGCGGACGTAGACGCGGCGGCTGATGCGGCGGCGCGCATCGCGGAAGATCGGGGCGGCTATTAC
GTCAACCAGTTCCACGCGCCGGAGTGTATGCGCGCCCACCGCGAAACGACCGGGCCGGAGATATGGGAGCAATCCTCCGG
CCAGGTCGATGCCTGGGTGGCCGCCGTAGGGACCGGTGCGACTTTCCTCGGAGTAGCGGCGGCATTGAGGGAACGTAATC
CCCACGTGATCTGCGCCGCCGTCGAACCGGAGGGATGTCGCCCCCTGGCGGGATTATCAGTCACAAAGCACCGCCACCTC
ATCCAGGGCACAAGCTACGGAAGCATCCCCCCACACTGGAATGGTGATCTGATGGACCTGTCTATTCCCGTAACCGATGA
GGAGGTGGAGCGCTGGCGTAGAGGGCTCGCGACGCAGGAGGGCTTGCACGTCGGCTACTCGGCCGCTGCCAACGTAGCTG
CGGCTGCTGCGCTGTTGTCGTCCGGACGCCTTCCAGCCGACGCAATCGCCGTGACCGTGCTGTGCGATACAGGCTTGAAA
TACTGA

Upstream 100 bases:

>100_bases
GGCGGATGAACTGAAGCGGACACGGCGGCGGGACATGCTCACGACCGCGAGCGGACCTCGATATCTCCCGAAGGCTGGAT
GCTCCTGCTACGATCCCTCG

Downstream 100 bases:

>100_bases
GGCCCACATCCCGCGCCTATCACGCCGATCCGAGGTTGCCGGACGGAGACGAATGTCCGCTTCCGGGAAGCGCCCGCGGC
GGTGCGGGCGACTGACATGG

Product: pyridoxal-5'-phosphate-dependent protein subunit beta

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRPTSLSPSPSTARIKPSALDLIGHTPLIALDRIHRGPGRILAKAEFMQPGGSVKDRAARAIVLAAREDGRLAPGAPVVE
MTSGNMGAGLAVACAALGHPLVVTMSAGNSPQRARMLEALGAEVILVPQIDGAAGQVTGADVDAAADAAARIAEDRGGYY
VNQFHAPECMRAHRETTGPEIWEQSSGQVDAWVAAVGTGATFLGVAAALRERNPHVICAAVEPEGCRPLAGLSVTKHRHL
IQGTSYGSIPPHWNGDLMDLSIPVTDEEVERWRRGLATQEGLHVGYSAAANVAAAAALLSSGRLPADAIAVTVLCDTGLK
Y

Sequences:

>Translated_321_residues
MRPTSLSPSPSTARIKPSALDLIGHTPLIALDRIHRGPGRILAKAEFMQPGGSVKDRAARAIVLAAREDGRLAPGAPVVE
MTSGNMGAGLAVACAALGHPLVVTMSAGNSPQRARMLEALGAEVILVPQIDGAAGQVTGADVDAAADAAARIAEDRGGYY
VNQFHAPECMRAHRETTGPEIWEQSSGQVDAWVAAVGTGATFLGVAAALRERNPHVICAAVEPEGCRPLAGLSVTKHRHL
IQGTSYGSIPPHWNGDLMDLSIPVTDEEVERWRRGLATQEGLHVGYSAAANVAAAAALLSSGRLPADAIAVTVLCDTGLK
Y
>Mature_321_residues
MRPTSLSPSPSTARIKPSALDLIGHTPLIALDRIHRGPGRILAKAEFMQPGGSVKDRAARAIVLAAREDGRLAPGAPVVE
MTSGNMGAGLAVACAALGHPLVVTMSAGNSPQRARMLEALGAEVILVPQIDGAAGQVTGADVDAAADAAARIAEDRGGYY
VNQFHAPECMRAHRETTGPEIWEQSSGQVDAWVAAVGTGATFLGVAAALRERNPHVICAAVEPEGCRPLAGLSVTKHRHL
IQGTSYGSIPPHWNGDLMDLSIPVTDEEVERWRRGLATQEGLHVGYSAAANVAAAAALLSSGRLPADAIAVTVLCDTGLK
Y

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=335, Percent_Identity=33.7313432835821, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI295821200, Length=335, Percent_Identity=33.7313432835821, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4557415, Length=335, Percent_Identity=33.7313432835821, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1788754, Length=305, Percent_Identity=38.0327868852459, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI2367138, Length=300, Percent_Identity=33.3333333333333, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI115535073, Length=310, Percent_Identity=38.7096774193548, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17535051, Length=297, Percent_Identity=37.037037037037, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32566674, Length=297, Percent_Identity=36.7003367003367, Blast_Score=154, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17562970, Length=297, Percent_Identity=36.7003367003367, Blast_Score=154, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17561720, Length=282, Percent_Identity=36.8794326241135, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17534315, Length=323, Percent_Identity=30.030959752322, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25147552, Length=317, Percent_Identity=30.5993690851735, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32566672, Length=211, Percent_Identity=34.5971563981043, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6321449, Length=332, Percent_Identity=33.1325301204819, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6321594, Length=331, Percent_Identity=29.3051359516616, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24643623, Length=276, Percent_Identity=30.7971014492754, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20129101, Length=276, Percent_Identity=30.7971014492754, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33310; Mature: 33310

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPTSLSPSPSTARIKPSALDLIGHTPLIALDRIHRGPGRILAKAEFMQPGGSVKDRAAR
CCCCCCCCCCCCCEECHHHHHHHCCCCEEEHHHHCCCCCCEEEEEECCCCCCCHHHHCCE
AIVLAAREDGRLAPGAPVVEMTSGNMGAGLAVACAALGHPLVVTMSAGNSPQRARMLEAL
EEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHC
GAEVILVPQIDGAAGQVTGADVDAAADAAARIAEDRGGYYVNQFHAPECMRAHRETTGPE
CCCEEEEECCCCCCCEECCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCH
IWEQSSGQVDAWVAAVGTGATFLGVAAALRERNPHVICAAVEPEGCRPLAGLSVTKHRHL
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHH
IQGTSYGSIPPHWNGDLMDLSIPVTDEEVERWRRGLATQEGLHVGYSAAANVAAAAALLS
CCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHH
SGRLPADAIAVTVLCDTGLKY
CCCCCHHHEEEEEEECCCCCC
>Mature Secondary Structure
MRPTSLSPSPSTARIKPSALDLIGHTPLIALDRIHRGPGRILAKAEFMQPGGSVKDRAAR
CCCCCCCCCCCCCEECHHHHHHHCCCCEEEHHHHCCCCCCEEEEEECCCCCCCHHHHCCE
AIVLAAREDGRLAPGAPVVEMTSGNMGAGLAVACAALGHPLVVTMSAGNSPQRARMLEAL
EEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHC
GAEVILVPQIDGAAGQVTGADVDAAADAAARIAEDRGGYYVNQFHAPECMRAHRETTGPE
CCCEEEEECCCCCCCEECCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCH
IWEQSSGQVDAWVAAVGTGATFLGVAAALRERNPHVICAAVEPEGCRPLAGLSVTKHRHL
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHH
IQGTSYGSIPPHWNGDLMDLSIPVTDEEVERWRRGLATQEGLHVGYSAAANVAAAAALLS
CCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHH
SGRLPADAIAVTVLCDTGLKY
CCCCCHHHEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA