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The map label for this gene is ribH

Identifier: 170750377

GI number: 170750377

Start: 4233416

End: 4233910

Strand: Reverse

Name: ribH

Synonym: Mrad2831_3980

Alternate gene names: 170750377

Gene position: 4233910-4233416 (Counterclockwise)

Preceding gene: 170750378

Following gene: 170750376

Centisome position: 69.66

GC content: 75.96

Gene sequence:

>495_bases
ATGGTCGCCCATACCCGCGCCGATTCGGCGCCCAAGCCCACCGACCCGGCGCTCGCCGGCGCCCGGATCCTCGTGGTCGA
GGCGCGCTACTACGACGCGATCGCCGACGAACTCCTCGCCGGCGCGCTGGCGGCGATCGAGGCCGCCGACGCCCGGGCCA
CCGTGGTGACCGTGCCGGGAGCCCTGGAGATCCCCGCCGCCGCGGCGATCCTGCTCGACACGGGGGCCTACGACGCCGTG
GTGGCGCTGGGCTGCGTCATCCGCGGCGAGACCGGCCACTACGACATCGTCGCCGGCGAGAGCGCCCGGGCGCTCATGGA
CCTGTCGGTGCAGCGCCGGGTGCCCCTGGGCAACGGCATCCTCACGGTCGAGACCGAGGCGCAGGCCCTCGCCCGCGCCC
GGGTCGCCGAGATGAACAAGGGCGGCGGTGCCGCCGAGGCGGCGCTCGGCGTGCTCGCCCTGAAGCGTATGGCCGACGGC
GCGCGCCCGCGATGA

Upstream 100 bases:

>100_bases
GCCGCTTGTGGCGCCGGCCGCTTCGGTCTAACCGTCCTCCCCCGTGCGGGTGCCCGACGTGCCGCCCGCCCATTCCCGAA
AACCCGGCCGGACGAGAGCC

Downstream 100 bases:

>100_bases
CGGATACACCCATGACCGGGACCCCCGACACCCATCCGGAGACCGGCACCGAAACCGCCGGCGCTCCCGCCAAGATCAGC
CCGCGCAGCGGCGCGCGCCT

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MVAHTRADSAPKPTDPALAGARILVVEARYYDAIADELLAGALAAIEAADARATVVTVPGALEIPAAAAILLDTGAYDAV
VALGCVIRGETGHYDIVAGESARALMDLSVQRRVPLGNGILTVETEAQALARARVAEMNKGGGAAEAALGVLALKRMADG
ARPR

Sequences:

>Translated_164_residues
MVAHTRADSAPKPTDPALAGARILVVEARYYDAIADELLAGALAAIEAADARATVVTVPGALEIPAAAAILLDTGAYDAV
VALGCVIRGETGHYDIVAGESARALMDLSVQRRVPLGNGILTVETEAQALARARVAEMNKGGGAAEAALGVLALKRMADG
ARPR
>Mature_164_residues
MVAHTRADSAPKPTDPALAGARILVVEARYYDAIADELLAGALAAIEAADARATVVTVPGALEIPAAAAILLDTGAYDAV
VALGCVIRGETGHYDIVAGESARALMDLSVQRRVPLGNGILTVETEAQALARARVAEMNKGGGAAEAALGVLALKRMADG
ARPR

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=138, Percent_Identity=44.2028985507246, Blast_Score=100, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6324429, Length=136, Percent_Identity=33.8235294117647, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_METRJ (B1LZB2)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001756637.1
- ProteinModelPortal:   B1LZB2
- SMR:   B1LZB2
- GeneID:   6140035
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_3980
- HOGENOM:   HBG311126
- OMA:   LGLQGAC
- ProtClustDB:   CLSK2331551
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16679; Mature: 16679

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAHTRADSAPKPTDPALAGARILVVEARYYDAIADELLAGALAAIEAADARATVVTVPG
CCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
ALEIPAAAAILLDTGAYDAVVALGCVIRGETGHYDIVAGESARALMDLSVQRRVPLGNGI
CCCCCCEEEEEEECCCHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCE
LTVETEAQALARARVAEMNKGGGAAEAALGVLALKRMADGARPR
EEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVAHTRADSAPKPTDPALAGARILVVEARYYDAIADELLAGALAAIEAADARATVVTVPG
CCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
ALEIPAAAAILLDTGAYDAVVALGCVIRGETGHYDIVAGESARALMDLSVQRRVPLGNGI
CCCCCCEEEEEEECCCHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCE
LTVETEAQALARARVAEMNKGGGAAEAALGVLALKRMADGARPR
EEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA