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The map label for this gene is glyA

Identifier: 170750353

GI number: 170750353

Start: 4208917

End: 4210221

Strand: Direct

Name: glyA

Synonym: Mrad2831_3956

Alternate gene names: 170750353

Gene position: 4208917-4210221 (Clockwise)

Preceding gene: 170750352

Following gene: 170750354

Centisome position: 69.25

GC content: 69.66

Gene sequence:

>1305_bases
ATGAGCGCCGGTACCGCCGCCGACAAGCACTTCTCCAACAGCTTCTTCGCCGCCCCGCTGACGGAAGCCGATCCCGAGAT
CGCCGAGGCGGTGGCCAAGGAGCTCGGCCGCCAGCAGCACGAGATCGAGCTGATCGCCTCGGAGAACATCGTCTCCCGCG
CCGTGCTGGAGGCTCAGGGGTCGGTGCTCACCAACAAGTACGCCGAGGGCTATCCGGGCCGGCGCTACTACGGCGGCTGC
CAGTTCGTGGACATCGCCGAGGACCTCGCCATCGAGCGCGCCAAGCGCCTGTTCGATTGCGGCTTCGCCAACGTGCAGCC
GAATTCCGGCTCGCAGGCCAACCAGGGCGTGTTCCTGGCCCTGATGCAGCCCGGCGACACGTTCCTGGGCCTCGACCTCG
CGGCCGGCGGCCACCTCACCCACGGCGCCCCGCCGAACGTCTCGGGCAAGTGGTTCAAGCCGGTCTCCTACACGGTGCGC
CGCGAGGACCAGCGGATCGACATGGAGCAGGTCGCCCAGCTCGCCCAGGAGCACAAGCCGAAGGTGATCATCGCCGGCGG
CTCGGGCTACCCGCGCCACTGGGACTTCGCGAAGTTCCGCGAGATCGCCGACTCGGTCGGCGCCTACTTCATGGTCGACA
TGGCCCATTTCGCGGGCCTCGTGGCGGCCGGCGTCCACCCGTCGCCGTTCCCGCACGCCCACGTCGCCACCACGACGACC
CACAAGACGCTCCGCGGCCCGCGCGGCGGCATGATCCTGACGAACGACGAGGCGCTCGCCAAGAAGTTCAACTCGGCGAT
CTTCCCCGGCCTCCAGGGCGGCCCGCTGATGCACGTCATCGCCGGCAAGGCGGTGGCCTTCGGCGAGGCGCTGAAGCCCG
AGTTCAAGATCTACGCCCGCCAGGTGGTGGAGAACGCCCGGGCGCTCGCCGACACGCTGATCTCGGGCGGCTACGACATC
ACCTCGGGCGGTACCGACAACCACCTGATGCTGGTCGACCTCCAGCGGAAGGGCCTCACCGGCAAGGCCGCCGAGGCGGC
CCTGTCGCGGGCGCACATCACCTGCAACAAGAACGGCGTCCCCTTCGACACGCAGAAGCCGACCATCACGTCGGGCATCC
GGCTCGGCACCCCGGCCGGCACCTCGCGCGGCTTCGGCGTCGCCGAGTTCAAGCAGATCGGCGGCTTCATCGTCGAGGTG
CTGGACGGGCTCGCCGCCAAGGGTGAGGCCGGCGACAGCGCGGTCGAGGCCGACGTCAAGACCCGCGTCCACGCGCTGAC
GGACCGGTTCCCGATCTACGGCTGA

Upstream 100 bases:

>100_bases
GGCCTGAGCCCTCGGCGCGTGTAGGAATCCCACCTTCAACGCCCGCCCGTGCACGCCGACGAAAGGCCGGGCATCGCCCC
TACCAGAACAGAGGCAGATC

Downstream 100 bases:

>100_bases
GGCGAGAGAGCCGCTTGAGCGGGGCCGGTCTTCCGGGCCGGCCCGCGGTGGATTAGGAAACGGCCGCGGATCCGACCGGT
CCGCGGCCGTTCCCATGCCC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSAGTAADKHFSNSFFAAPLTEADPEIAEAVAKELGRQQHEIELIASENIVSRAVLEAQGSVLTNKYAEGYPGRRYYGGC
QFVDIAEDLAIERAKRLFDCGFANVQPNSGSQANQGVFLALMQPGDTFLGLDLAAGGHLTHGAPPNVSGKWFKPVSYTVR
REDQRIDMEQVAQLAQEHKPKVIIAGGSGYPRHWDFAKFREIADSVGAYFMVDMAHFAGLVAAGVHPSPFPHAHVATTTT
HKTLRGPRGGMILTNDEALAKKFNSAIFPGLQGGPLMHVIAGKAVAFGEALKPEFKIYARQVVENARALADTLISGGYDI
TSGGTDNHLMLVDLQRKGLTGKAAEAALSRAHITCNKNGVPFDTQKPTITSGIRLGTPAGTSRGFGVAEFKQIGGFIVEV
LDGLAAKGEAGDSAVEADVKTRVHALTDRFPIYG

Sequences:

>Translated_434_residues
MSAGTAADKHFSNSFFAAPLTEADPEIAEAVAKELGRQQHEIELIASENIVSRAVLEAQGSVLTNKYAEGYPGRRYYGGC
QFVDIAEDLAIERAKRLFDCGFANVQPNSGSQANQGVFLALMQPGDTFLGLDLAAGGHLTHGAPPNVSGKWFKPVSYTVR
REDQRIDMEQVAQLAQEHKPKVIIAGGSGYPRHWDFAKFREIADSVGAYFMVDMAHFAGLVAAGVHPSPFPHAHVATTTT
HKTLRGPRGGMILTNDEALAKKFNSAIFPGLQGGPLMHVIAGKAVAFGEALKPEFKIYARQVVENARALADTLISGGYDI
TSGGTDNHLMLVDLQRKGLTGKAAEAALSRAHITCNKNGVPFDTQKPTITSGIRLGTPAGTSRGFGVAEFKQIGGFIVEV
LDGLAAKGEAGDSAVEADVKTRVHALTDRFPIYG
>Mature_433_residues
SAGTAADKHFSNSFFAAPLTEADPEIAEAVAKELGRQQHEIELIASENIVSRAVLEAQGSVLTNKYAEGYPGRRYYGGCQ
FVDIAEDLAIERAKRLFDCGFANVQPNSGSQANQGVFLALMQPGDTFLGLDLAAGGHLTHGAPPNVSGKWFKPVSYTVRR
EDQRIDMEQVAQLAQEHKPKVIIAGGSGYPRHWDFAKFREIADSVGAYFMVDMAHFAGLVAAGVHPSPFPHAHVATTTTH
KTLRGPRGGMILTNDEALAKKFNSAIFPGLQGGPLMHVIAGKAVAFGEALKPEFKIYARQVVENARALADTLISGGYDIT
SGGTDNHLMLVDLQRKGLTGKAAEAALSRAHITCNKNGVPFDTQKPTITSGIRLGTPAGTSRGFGVAEFKQIGGFIVEVL
DGLAAKGEAGDSAVEADVKTRVHALTDRFPIYG

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=453, Percent_Identity=45.916114790287, Blast_Score=354, Evalue=8e-98,
Organism=Homo sapiens, GI261862350, Length=453, Percent_Identity=45.916114790287, Blast_Score=354, Evalue=8e-98,
Organism=Homo sapiens, GI261862348, Length=453, Percent_Identity=45.916114790287, Blast_Score=354, Evalue=8e-98,
Organism=Homo sapiens, GI19923315, Length=453, Percent_Identity=45.916114790287, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI22547186, Length=438, Percent_Identity=47.2602739726027, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI261862346, Length=453, Percent_Identity=45.2538631346578, Blast_Score=344, Evalue=8e-95,
Organism=Homo sapiens, GI22547189, Length=421, Percent_Identity=44.8931116389549, Blast_Score=312, Evalue=5e-85,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=58.6538461538462, Blast_Score=461, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI25144732, Length=455, Percent_Identity=44.8351648351648, Blast_Score=348, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI25144729, Length=455, Percent_Identity=44.8351648351648, Blast_Score=347, Evalue=8e-96,
Organism=Saccharomyces cerevisiae, GI6319739, Length=436, Percent_Identity=43.1192660550459, Blast_Score=350, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6323087, Length=416, Percent_Identity=43.5096153846154, Blast_Score=323, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24640005, Length=424, Percent_Identity=47.6415094339623, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI221329721, Length=405, Percent_Identity=48.641975308642, Blast_Score=370, Evalue=1e-102,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_METRJ (B1LZ88)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001756613.1
- ProteinModelPortal:   B1LZ88
- SMR:   B1LZ88
- GeneID:   6140011
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_3956
- HOGENOM:   HBG301263
- OMA:   IDMEQVA
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46270; Mature: 46139

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGTAADKHFSNSFFAAPLTEADPEIAEAVAKELGRQQHEIELIASENIVSRAVLEAQG
CCCCCCCHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCHHEEEEEECCCHHHHHHHHHCC
SVLTNKYAEGYPGRRYYGGCQFVDIAEDLAIERAKRLFDCGFANVQPNSGSQANQGVFLA
CHHHHHHHCCCCCCEECCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
LMQPGDTFLGLDLAAGGHLTHGAPPNVSGKWFKPVSYTVRREDQRIDMEQVAQLAQEHKP
EECCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCHHEEECCCCCCCHHHHHHHHHHCCC
KVIIAGGSGYPRHWDFAKFREIADSVGAYFMVDMAHFAGLVAAGVHPSPFPHAHVATTTT
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCC
HKTLRGPRGGMILTNDEALAKKFNSAIFPGLQGGPLMHVIAGKAVAFGEALKPEFKIYAR
HHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEHHCCHHHHHHHCCHHHHHHHH
QVVENARALADTLISGGYDITSGGTDNHLMLVDLQRKGLTGKAAEAALSRAHITCNKNGV
HHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEECCCCC
PFDTQKPTITSGIRLGTPAGTSRGFGVAEFKQIGGFIVEVLDGLAAKGEAGDSAVEADVK
CCCCCCCCHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
TRVHALTDRFPIYG
HHHHHHHCCCCCCC
>Mature Secondary Structure 
SAGTAADKHFSNSFFAAPLTEADPEIAEAVAKELGRQQHEIELIASENIVSRAVLEAQG
CCCCCCHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCHHEEEEEECCCHHHHHHHHHCC
SVLTNKYAEGYPGRRYYGGCQFVDIAEDLAIERAKRLFDCGFANVQPNSGSQANQGVFLA
CHHHHHHHCCCCCCEECCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
LMQPGDTFLGLDLAAGGHLTHGAPPNVSGKWFKPVSYTVRREDQRIDMEQVAQLAQEHKP
EECCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCHHEEECCCCCCCHHHHHHHHHHCCC
KVIIAGGSGYPRHWDFAKFREIADSVGAYFMVDMAHFAGLVAAGVHPSPFPHAHVATTTT
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCC
HKTLRGPRGGMILTNDEALAKKFNSAIFPGLQGGPLMHVIAGKAVAFGEALKPEFKIYAR
HHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEHHCCHHHHHHHCCHHHHHHHH
QVVENARALADTLISGGYDITSGGTDNHLMLVDLQRKGLTGKAAEAALSRAHITCNKNGV
HHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEECCCCC
PFDTQKPTITSGIRLGTPAGTSRGFGVAEFKQIGGFIVEVLDGLAAKGEAGDSAVEADVK
CCCCCCCCHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
TRVHALTDRFPIYG
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA