Click here to switch to the map view.

The map label for this gene is ybdK [C]

Identifier: 170750288

GI number: 170750288

Start: 4138278

End: 4139396

Strand: Reverse

Name: ybdK [C]

Synonym: Mrad2831_3891

Alternate gene names: 170750288

Gene position: 4139396-4138278 (Counterclockwise)

Preceding gene: 170750289

Following gene: 170750284

Centisome position: 68.11

GC content: 75.6

Gene sequence:

>1119_bases
ATGAGCGCCCCGAGCTACCGCTTCGGCATCGAGGAGGAGTACTTCCTGGCCGATGCCGAGACCCGCGGGACGCCGCGGGG
CGACCTCGCCGCGTTCCACGCGCGGGTGGAGGCGGAGCTGCCCGAGACGGGCCGCGAGCTGGTCGCCGCGCAGGTCGAGC
TCTGCACGCCGCCGCTCACCGACGCGGCGGCCGCCCGGGAGAATCTCGGCGGCCAGCGGCGGCAGCTCGCCGGCATCGCC
GCCGAGAGCGGCCTGCGGCTGCTGGCCTGCGGCACGCACCCCCTCGCCCGCTGGTCGCGCCAGACCGCCACCGACGGGGA
GCGCTACAAGGGCATCCTGTCGGATGTCGGCATCGCGGCCCGGCGGGCCTTGATCTGCGGCATGCACGTCCACGTCGAGG
TTCCAGACCCCGACGCCCGCGTCGACCTGATGAACCGGCTGCTGCCGTTCCAGCCGCTGCTGCTCGCGCTGTCGGCCTCC
TCGCCGTTCTGGCAGGGCGAGGCGACGGGCCTGCGGGCCTACCGGCTCAGCGTCTTCGGGGAATTGCCGCGCACCGGCCT
GCCGGAACTCTTCGCCGACGCCGCCGCCCACGCGCGGTTCGTCGCCATCATGACCCGGTCGGGCGCGATCGCGGACGCGA
GCTTCCTGTGGTGGTCGCTGCGCCCCTCCATCAAGTTCCCGACGCTGGAGCTGCGCATCGCCGACGCCTGCACGCGCCTC
GACGATTCGCTCTGCATCGCCGCCCTGTTCCGCTGCCTCGTGCGCCTGTGCGTGCGCCGGCCGGAGCTCCACGCCGGCCT
CGACGGCGTCTCGCGGGCGCTGGCCTCGGAGAACCTCTGGCGCGCCCAGCACAGCGGCGTGGCCGCGTCCCTGATCGACG
AGGCGCGCGGCGAGGCCGTGCCGTTCGCCGAGGCGCTGGAGGCGATGCTCACGCTCGTCGCCGAGGACGCGGACGCCCTC
GGCTGCGCGGCGGACGTGGCCCGCGCCCGGGCGATCGTGGCCGAGGGGACCAGCGCCGACGGGCAGGTCGCGGCCTACGA
GGCCGCGCTCACCGCGGGTCAGGACGAGCGCGCCGCGCTGGCGGCCGCGGTGGACTGGATCGCCGACGCGGCGCGCTGA

Upstream 100 bases:

>100_bases
CCCGCTTGGCTGGTCGGCGGGCCGCTGCGGACGCCCTGAGCGGCACCTTCCTGCAATCCGGCGCGTTGGCGCGCACGCGT
CGCGTTTTCCGGAGACCACG

Downstream 100 bases:

>100_bases
GGGCTGCGCAGGGCTGCCGCATCGCCTCCGTCATCACGAGCGGAGGTTGGATCAGCCCTTGTCGAACGGGTTCTGCGAGG
TCCGCAGGGACAGGCGGATC

Product: glutamate--cysteine ligase GCS2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSAPSYRFGIEEEYFLADAETRGTPRGDLAAFHARVEAELPETGRELVAAQVELCTPPLTDAAAARENLGGQRRQLAGIA
AESGLRLLACGTHPLARWSRQTATDGERYKGILSDVGIAARRALICGMHVHVEVPDPDARVDLMNRLLPFQPLLLALSAS
SPFWQGEATGLRAYRLSVFGELPRTGLPELFADAAAHARFVAIMTRSGAIADASFLWWSLRPSIKFPTLELRIADACTRL
DDSLCIAALFRCLVRLCVRRPELHAGLDGVSRALASENLWRAQHSGVAASLIDEARGEAVPFAEALEAMLTLVAEDADAL
GCAADVARARAIVAEGTSADGQVAAYEAALTAGQDERAALAAAVDWIADAAR

Sequences:

>Translated_372_residues
MSAPSYRFGIEEEYFLADAETRGTPRGDLAAFHARVEAELPETGRELVAAQVELCTPPLTDAAAARENLGGQRRQLAGIA
AESGLRLLACGTHPLARWSRQTATDGERYKGILSDVGIAARRALICGMHVHVEVPDPDARVDLMNRLLPFQPLLLALSAS
SPFWQGEATGLRAYRLSVFGELPRTGLPELFADAAAHARFVAIMTRSGAIADASFLWWSLRPSIKFPTLELRIADACTRL
DDSLCIAALFRCLVRLCVRRPELHAGLDGVSRALASENLWRAQHSGVAASLIDEARGEAVPFAEALEAMLTLVAEDADAL
GCAADVARARAIVAEGTSADGQVAAYEAALTAGQDERAALAAAVDWIADAAR
>Mature_371_residues
SAPSYRFGIEEEYFLADAETRGTPRGDLAAFHARVEAELPETGRELVAAQVELCTPPLTDAAAARENLGGQRRQLAGIAA
ESGLRLLACGTHPLARWSRQTATDGERYKGILSDVGIAARRALICGMHVHVEVPDPDARVDLMNRLLPFQPLLLALSASS
PFWQGEATGLRAYRLSVFGELPRTGLPELFADAAAHARFVAIMTRSGAIADASFLWWSLRPSIKFPTLELRIADACTRLD
DSLCIAALFRCLVRLCVRRPELHAGLDGVSRALASENLWRAQHSGVAASLIDEARGEAVPFAEALEAMLTLVAEDADALG
CAADVARARAIVAEGTSADGQVAAYEAALTAGQDERAALAAAVDWIADAAR

Specific function: ATP-dependent carboxylate-amine ligase [H]

COG id: COG2170

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carboxylate-amine ligase family [H]

Homologues:

Organism=Escherichia coli, GI1786795, Length=295, Percent_Identity=26.7796610169492, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011793
- InterPro:   IPR006336 [H]

Pfam domain/function: PF04107 GCS2 [H]

EC number: NA

Molecular weight: Translated: 39720; Mature: 39589

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPSYRFGIEEEYFLADAETRGTPRGDLAAFHARVEAELPETGRELVAAQVELCTPPLT
CCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
DAAAARENLGGQRRQLAGIAAESGLRLLACGTHPLARWSRQTATDGERYKGILSDVGIAA
HHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHH
RRALICGMHVHVEVPDPDARVDLMNRLLPFQPLLLALSASSPFWQGEATGLRAYRLSVFG
HHHHHEEEEEEEECCCCCHHHHHHHHHCCCCHHHHEEECCCCCCCCCCCCHHHEEEHHHH
ELPRTGLPELFADAAAHARFVAIMTRSGAIADASFLWWSLRPSIKFPTLELRIADACTRL
CCCCCCCHHHHHHHHHHHEEEEEEECCCCCCCHHHEEEECCCCCCCCEEEEEHHHHHHHC
DDSLCIAALFRCLVRLCVRRPELHAGLDGVSRALASENLWRAQHSGVAASLIDEARGEAV
CHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
PFAEALEAMLTLVAEDADALGCAADVARARAIVAEGTSADGQVAAYEAALTAGQDERAAL
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCEEHHHHHHHCCCCHHHHH
AAAVDWIADAAR
HHHHHHHHHCCC
>Mature Secondary Structure 
SAPSYRFGIEEEYFLADAETRGTPRGDLAAFHARVEAELPETGRELVAAQVELCTPPLT
CCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
DAAAARENLGGQRRQLAGIAAESGLRLLACGTHPLARWSRQTATDGERYKGILSDVGIAA
HHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHH
RRALICGMHVHVEVPDPDARVDLMNRLLPFQPLLLALSASSPFWQGEATGLRAYRLSVFG
HHHHHEEEEEEEECCCCCHHHHHHHHHCCCCHHHHEEECCCCCCCCCCCCHHHEEEHHHH
ELPRTGLPELFADAAAHARFVAIMTRSGAIADASFLWWSLRPSIKFPTLELRIADACTRL
CCCCCCCHHHHHHHHHHHEEEEEEECCCCCCCHHHEEEECCCCCCCCEEEEEHHHHHHHC
DDSLCIAALFRCLVRLCVRRPELHAGLDGVSRALASENLWRAQHSGVAASLIDEARGEAV
CHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
PFAEALEAMLTLVAEDADALGCAADVARARAIVAEGTSADGQVAAYEAALTAGQDERAAL
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCEEHHHHHHHCCCCHHHHH
AAAVDWIADAAR
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA