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The map label for this gene is phnD [H]

Identifier: 170750259

GI number: 170750259

Start: 4109961

End: 4110887

Strand: Reverse

Name: phnD [H]

Synonym: Mrad2831_3861

Alternate gene names: 170750259

Gene position: 4110887-4109961 (Counterclockwise)

Preceding gene: 170750260

Following gene: 170750258

Centisome position: 67.64

GC content: 68.07

Gene sequence:

>927_bases
ATGATCACCCGACGCACCCTCGCCGCCGCCGCGGCCCTCGCCCTCCTCGGCCTGCCGGCCGCCGCCCAGGACTGGAAGGC
CCAGTATCCGGAGCTGACGCTCGGCGTGATCCCGGCGGAGAACGCCTCGGGCACCGCCGACCGGTGGGCGCCGCTCACCG
CCTACCTGACCAAGACGCTCGGCGTGCCGGTGAAGCTGCGGGTGGCGAGCGACTACGCCGCCGTGATCGAGGGCCAGCGG
GCCGGCAACATCCAGATCGCGTGGTACGGGCCGGCCTCCTTCGCGCGGGCCGTGATGACCGGCGTGAAGATCGAGCCGAT
CGTCAACCAGAAGCACGAGACCGGCGCCTCGGGCTACTATTCGGTGGTCTACGTGCTGGCCTCCAGCCCCTACAAGACCG
TCGAGGATCTGAAGGGCAAGAACCTCGCCCTGGTCGATCCGAACTCGACCTCGGGCAACCAGGCGCCGCGCTTCTTCCTG
AAGCGGGCCGGCTACGACGTAGAGAAGTTCTTCGGCAAGACCTTCTACGCCGGCAGCCACGAGAACGCCGTGCTGGCGCT
CACCCAGGGCACCGCCGACGCCGCCGCCAACCTGTGGAATTCCGAGACCGACACGATGGTCACCCGCATGGCCGCCAAGG
GCATGCTAAAGAAGCCCGACGGCACCCCGATGCAGCAGTCGGACTTCCGCGTCGTGTTCAAGTCCGACTTCCTGCCGGAG
GGGCCATTCGCCGTGCTGGCGAGCCTGCCGGACGACCTGAAGGCCAAGATCCGCGACGCCTTCGTGGCCCTGCCGAAGGC
CGACAAGGCCGCGTTCGACCGGCTCTCCGACGGCAAGGATCAGGACCTCACGCCGGTGACCCTCAAGGATTACCAGCCGA
TCATCGACATGCTGCGGTTCAACGACGAGCAGCGCCGGAAGTCCTGA

Upstream 100 bases:

>100_bases
AACACTGCCAACGAGCACTGCCAACGAGCACCGCTCTGGAAACGCCCGACCGGCCCTTGGCCGGCCGAACCTCGCCGACG
CCGCCACGAAGGACGACCCG

Downstream 100 bases:

>100_bases
TCCGGAGCCGCCCGCCTTGCGCGGCCTGACACCCCTGTCGCCGGAGCGGGCAGCCGCCCTCTCCGGCCTCTACGCGGCGG
CGACCGGGGAAGCCCGCCGC

Product: phosphonate ABC transporter periplasmic phosphonate binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MITRRTLAAAAALALLGLPAAAQDWKAQYPELTLGVIPAENASGTADRWAPLTAYLTKTLGVPVKLRVASDYAAVIEGQR
AGNIQIAWYGPASFARAVMTGVKIEPIVNQKHETGASGYYSVVYVLASSPYKTVEDLKGKNLALVDPNSTSGNQAPRFFL
KRAGYDVEKFFGKTFYAGSHENAVLALTQGTADAAANLWNSETDTMVTRMAAKGMLKKPDGTPMQQSDFRVVFKSDFLPE
GPFAVLASLPDDLKAKIRDAFVALPKADKAAFDRLSDGKDQDLTPVTLKDYQPIIDMLRFNDEQRRKS

Sequences:

>Translated_308_residues
MITRRTLAAAAALALLGLPAAAQDWKAQYPELTLGVIPAENASGTADRWAPLTAYLTKTLGVPVKLRVASDYAAVIEGQR
AGNIQIAWYGPASFARAVMTGVKIEPIVNQKHETGASGYYSVVYVLASSPYKTVEDLKGKNLALVDPNSTSGNQAPRFFL
KRAGYDVEKFFGKTFYAGSHENAVLALTQGTADAAANLWNSETDTMVTRMAAKGMLKKPDGTPMQQSDFRVVFKSDFLPE
GPFAVLASLPDDLKAKIRDAFVALPKADKAAFDRLSDGKDQDLTPVTLKDYQPIIDMLRFNDEQRRKS
>Mature_308_residues
MITRRTLAAAAALALLGLPAAAQDWKAQYPELTLGVIPAENASGTADRWAPLTAYLTKTLGVPVKLRVASDYAAVIEGQR
AGNIQIAWYGPASFARAVMTGVKIEPIVNQKHETGASGYYSVVYVLASSPYKTVEDLKGKNLALVDPNSTSGNQAPRFFL
KRAGYDVEKFFGKTFYAGSHENAVLALTQGTADAAANLWNSETDTMVTRMAAKGMLKKPDGTPMQQSDFRVVFKSDFLPE
GPFAVLASLPDDLKAKIRDAFVALPKADKAAFDRLSDGKDQDLTPVTLKDYQPIIDMLRFNDEQRRKS

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for phosphate binding [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790543, Length=281, Percent_Identity=29.8932384341637, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 33455; Mature: 33455

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRRTLAAAAALALLGLPAAAQDWKAQYPELTLGVIPAENASGTADRWAPLTAYLTKTL
CCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHC
GVPVKLRVASDYAAVIEGQRAGNIQIAWYGPASFARAVMTGVKIEPIVNQKHETGASGYY
CCCEEEEECCCCEEHHCCCCCCCEEEEEECHHHHHHHHHHCCEEECCCCCCCCCCCCCCE
SVVYVLASSPYKTVEDLKGKNLALVDPNSTSGNQAPRFFLKRAGYDVEKFFGKTFYAGSH
EEEEEEECCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHCCCEECCCC
ENAVLALTQGTADAAANLWNSETDTMVTRMAAKGMLKKPDGTPMQQSDFRVVFKSDFLPE
CCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCC
GPFAVLASLPDDLKAKIRDAFVALPKADKAAFDRLSDGKDQDLTPVTLKDYQPIIDMLRF
CCHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHC
NDEQRRKS
CCHHHHCC
>Mature Secondary Structure
MITRRTLAAAAALALLGLPAAAQDWKAQYPELTLGVIPAENASGTADRWAPLTAYLTKTL
CCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHC
GVPVKLRVASDYAAVIEGQRAGNIQIAWYGPASFARAVMTGVKIEPIVNQKHETGASGYY
CCCEEEEECCCCEEHHCCCCCCCEEEEEECHHHHHHHHHHCCEEECCCCCCCCCCCCCCE
SVVYVLASSPYKTVEDLKGKNLALVDPNSTSGNQAPRFFLKRAGYDVEKFFGKTFYAGSH
EEEEEEECCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHCCCEECCCC
ENAVLALTQGTADAAANLWNSETDTMVTRMAAKGMLKKPDGTPMQQSDFRVVFKSDFLPE
CCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCC
GPFAVLASLPDDLKAKIRDAFVALPKADKAAFDRLSDGKDQDLTPVTLKDYQPIIDMLRF
CCHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHC
NDEQRRKS
CCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA