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The map label for this gene is greA [H]

Identifier: 170750231

GI number: 170750231

Start: 4078199

End: 4078672

Strand: Reverse

Name: greA [H]

Synonym: Mrad2831_3833

Alternate gene names: 170750231

Gene position: 4078672-4078199 (Counterclockwise)

Preceding gene: 170750233

Following gene: 170750229

Centisome position: 67.11

GC content: 68.99

Gene sequence:

>474_bases
ATGAGCGTCGCATTCGTCAAAGAGCCCGAGGGTGGGGAAGCGTTCGAGAATCTGCCGGATCGGCCGATCTCGCCGCACAC
GAACTTCGTCACGCCGGAGGGCCTCGCGCAGATCGAGGCCGAGGTCGCGCGCCTCGAGAAGGAGGTCGCCTCGCTGGATC
CCGCCGACAAGGCGGAGGATTCCCGCATCGGGCGCGACCTGCGCTACTGGGCGGCCCGGAAGAACACCGCGCAGCTCGTG
GAGCCCAAGGGCGGCGACACCGTGCAGTTCGGGTCCACCGTCACGGTGGTCCGCGAGGACGAGTCCGGCAAGGAGACCCG
GCAGACCTTCCGGATCGTCGGCGAGGACGAGGCCGACCCGCACAAGGGCTCGATCTCGTACGTGGCGCCCCTCGCCCGGG
CGATCACCGGCAAGACGATCGGCGACGTGGTCGAGGTGAACGGCCACGAGGTCGAGGTGCTCGAGATCCGCTGA

Upstream 100 bases:

>100_bases
ATCTAGCGAGCGGTGCCCGGGTCGCGCCACGGCGAAAACACCGGGCGGGCCGAGCACCCGTCGCGGGAACCTTTGCCGTA
GCTCGCGTGTTGGCGTCTTC

Downstream 100 bases:

>100_bases
GCCGGATCAGCGGCCCTCGAAGCGCGCCCGCAGCGGCAGCAGCGCCTCGGCGACGTGCTCCATGAAGGCCCGGACACGCG
CCGCGTGGCGCAGGTCCGGA

Product: GreA/GreB family elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MSVAFVKEPEGGEAFENLPDRPISPHTNFVTPEGLAQIEAEVARLEKEVASLDPADKAEDSRIGRDLRYWAARKNTAQLV
EPKGGDTVQFGSTVTVVREDESGKETRQTFRIVGEDEADPHKGSISYVAPLARAITGKTIGDVVEVNGHEVEVLEIR

Sequences:

>Translated_157_residues
MSVAFVKEPEGGEAFENLPDRPISPHTNFVTPEGLAQIEAEVARLEKEVASLDPADKAEDSRIGRDLRYWAARKNTAQLV
EPKGGDTVQFGSTVTVVREDESGKETRQTFRIVGEDEADPHKGSISYVAPLARAITGKTIGDVVEVNGHEVEVLEIR
>Mature_156_residues
SVAFVKEPEGGEAFENLPDRPISPHTNFVTPEGLAQIEAEVARLEKEVASLDPADKAEDSRIGRDLRYWAARKNTAQLVE
PKGGDTVQFGSTVTVVREDESGKETRQTFRIVGEDEADPHKGSISYVAPLARAITGKTIGDVVEVNGHEVEVLEIR

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

None

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17140; Mature: 17009

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAFVKEPEGGEAFENLPDRPISPHTNFVTPEGLAQIEAEVARLEKEVASLDPADKAED
CCCEEEECCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SRIGRDLRYWAARKNTAQLVEPKGGDTVQFGSTVTVVREDESGKETRQTFRIVGEDEADP
HHHCCHHHHHHHCCCCCEEECCCCCCEEEECCEEEEEEECCCCHHHHHHHEEECCCCCCC
HKGSISYVAPLARAITGKTIGDVVEVNGHEVEVLEIR
CCCCHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEC
>Mature Secondary Structure 
SVAFVKEPEGGEAFENLPDRPISPHTNFVTPEGLAQIEAEVARLEKEVASLDPADKAED
CCEEEECCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SRIGRDLRYWAARKNTAQLVEPKGGDTVQFGSTVTVVREDESGKETRQTFRIVGEDEADP
HHHCCHHHHHHHCCCCCEEECCCCCCEEEECCEEEEEEECCCCHHHHHHHEEECCCCCCC
HKGSISYVAPLARAITGKTIGDVVEVNGHEVEVLEIR
CCCCHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA