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The map label for this gene is clcA [H]

Identifier: 170750158

GI number: 170750158

Start: 4000609

End: 4001988

Strand: Reverse

Name: clcA [H]

Synonym: Mrad2831_3760

Alternate gene names: 170750158

Gene position: 4001988-4000609 (Counterclockwise)

Preceding gene: 170750159

Following gene: 170750157

Centisome position: 65.85

GC content: 74.06

Gene sequence:

>1380_bases
ATGGTCGAGAAGCGGGACGAGGTGCGGGAAACGACGGATGCCCCGCCCCCGCAACGGTCGCGCTACCGACGCTCGATCAT
GCGCCTGCGGTCGGCCTCGATGGAGGCTTACCCACTCTGGCGCGGGCGGCTGCTCTTCCTCGTGGGCGGGATCGGCGTCG
GCCTCGCCGCCGTGCTGATGGCCAAGGGCGCCGACCTCGCGCAGGACGGGTTCCGGCGGCTCACGGCGCCGTCGCCGCTG
ATCGCCCTGGTGCTGACGCCCGCGGGCTTCGCCCTCGCGGCGCTCCTCGCCCTCACGGTCTTCCCGAACTCGCAGGGCTC
GGGCATCCCGCAGGTGATCGCCGCCCGCCACGCCCGGGATGCCGGCTTCCGCTACTCGCTGATCTCGCCGCGCGTGGCCT
TCGGCAAGGTGCTGGTCCTGTTCCTGGGCCTGTTCTGCGGTGCCTCGGCGGGCCGCGAGGGGCCGACCGTGCAGGTCGGC
GCCGCGATCATGGCCGCCTTCGGCCGCCTGACCCCCGAGCGCCTGCCCGGGCTCCTCCTCGCGGGCGGCGCCGCCGGCGT
CGCGGCGGCCTTCAACACGCCGCTCGCCGGCATCGTGTTCGGCATCGAGGAACTGGGACGCGCCTACGAGGCGCGCTCCT
CCGGCCTGATCGTGGCCGGGATCGTGGCCGCGGGCCTGACCTCGCTCGGGCTTCTCGGCAACTATACCTATTTCGGGACG
ACCGACGCCGCCCTGCCGCTGGCGGCCGGCTGGATCGTCCCGCTCGTCGCCGTCGTCGGCGGCCTCGCGGGCGGCCTGTT
CAGCCGCTTGGTGATCCTGTTCGCCCGCGGCCTGCCCGGCGCGGCCGGACGGGCGATCCGGAAGCGGCCCGTGATCTTCG
CGGCGGCCTGCGGGCTGTGCGTCGCCCTGTGCGGCCTCGCCTCCGGGGGCGCGGCCTACGGGACCGGCTACGAGGAGGCC
AAGGCCATCCTTCACGGCGACGCGGCGGCCGGCTGGACCTACGCGCCCCTGAAATTCGCCGCCACCGTGCTGAGCTCGAT
CAGCGGCATCCCGGGCGGCCTGTTCGCGCCGTCCCTGGCGGTCGGGGCCGGCCTCGGCGGCACGGTCCACGGCCTCCTGC
CGGGCATGCCCGTGGGCGCCGTCGTGCTGATCGGGATGGTGGCCTACCTGACCGGCGTGCTGCAGGCGCCGATCACCAGC
TTCGTCATCGTCACCGAGATGACCCAGAACCACGCGATGGTAATCCCCCTGATGGTCGCGGCCCTGCTGGCCGACGCGGC
CTCGAAGGCGATCTGCCGGGGCGGCCTCTACCACACCCTGGCGGAGATCATGCTCGAGCGCGCCGACGTGCCGGCGCAGC
CCGTGGCGAAGACCGGCTGA

Upstream 100 bases:

>100_bases
GGCACGCGGGTGCCGGCCTCCGGCCGTCCCGCGAAGGGGGTCTTTCCTTGACCGTTGCGCTATAGCGTCCGCTCCGACTG
CGCGGACGAGGTGCTCTTTC

Downstream 100 bases:

>100_bases
CCGCGCCGCGACCCGCGCGACCGAGCGCGGGTAAGAATAAAAGCGGACCGGGCCGGAATCCGCGGAACGGTCTCGCTTGG
CGCCGGTTGAGGCAGCAGCA

Product: chloride channel core

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MVEKRDEVRETTDAPPPQRSRYRRSIMRLRSASMEAYPLWRGRLLFLVGGIGVGLAAVLMAKGADLAQDGFRRLTAPSPL
IALVLTPAGFALAALLALTVFPNSQGSGIPQVIAARHARDAGFRYSLISPRVAFGKVLVLFLGLFCGASAGREGPTVQVG
AAIMAAFGRLTPERLPGLLLAGGAAGVAAAFNTPLAGIVFGIEELGRAYEARSSGLIVAGIVAAGLTSLGLLGNYTYFGT
TDAALPLAAGWIVPLVAVVGGLAGGLFSRLVILFARGLPGAAGRAIRKRPVIFAAACGLCVALCGLASGGAAYGTGYEEA
KAILHGDAAAGWTYAPLKFAATVLSSISGIPGGLFAPSLAVGAGLGGTVHGLLPGMPVGAVVLIGMVAYLTGVLQAPITS
FVIVTEMTQNHAMVIPLMVAALLADAASKAICRGGLYHTLAEIMLERADVPAQPVAKTG

Sequences:

>Translated_459_residues
MVEKRDEVRETTDAPPPQRSRYRRSIMRLRSASMEAYPLWRGRLLFLVGGIGVGLAAVLMAKGADLAQDGFRRLTAPSPL
IALVLTPAGFALAALLALTVFPNSQGSGIPQVIAARHARDAGFRYSLISPRVAFGKVLVLFLGLFCGASAGREGPTVQVG
AAIMAAFGRLTPERLPGLLLAGGAAGVAAAFNTPLAGIVFGIEELGRAYEARSSGLIVAGIVAAGLTSLGLLGNYTYFGT
TDAALPLAAGWIVPLVAVVGGLAGGLFSRLVILFARGLPGAAGRAIRKRPVIFAAACGLCVALCGLASGGAAYGTGYEEA
KAILHGDAAAGWTYAPLKFAATVLSSISGIPGGLFAPSLAVGAGLGGTVHGLLPGMPVGAVVLIGMVAYLTGVLQAPITS
FVIVTEMTQNHAMVIPLMVAALLADAASKAICRGGLYHTLAEIMLERADVPAQPVAKTG
>Mature_459_residues
MVEKRDEVRETTDAPPPQRSRYRRSIMRLRSASMEAYPLWRGRLLFLVGGIGVGLAAVLMAKGADLAQDGFRRLTAPSPL
IALVLTPAGFALAALLALTVFPNSQGSGIPQVIAARHARDAGFRYSLISPRVAFGKVLVLFLGLFCGASAGREGPTVQVG
AAIMAAFGRLTPERLPGLLLAGGAAGVAAAFNTPLAGIVFGIEELGRAYEARSSGLIVAGIVAAGLTSLGLLGNYTYFGT
TDAALPLAAGWIVPLVAVVGGLAGGLFSRLVILFARGLPGAAGRAIRKRPVIFAAACGLCVALCGLASGGAAYGTGYEEA
KAILHGDAAAGWTYAPLKFAATVLSSISGIPGGLFAPSLAVGAGLGGTVHGLLPGMPVGAVVLIGMVAYLTGVLQAPITS
FVIVTEMTQNHAMVIPLMVAALLADAASKAICRGGLYHTLAEIMLERADVPAQPVAKTG

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786350, Length=453, Percent_Identity=30.9050772626932, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 46674; Mature: 46674

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKRDEVRETTDAPPPQRSRYRRSIMRLRSASMEAYPLWRGRLLFLVGGIGVGLAAVLM
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AKGADLAQDGFRRLTAPSPLIALVLTPAGFALAALLALTVFPNSQGSGIPQVIAARHARD
HCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHC
AGFRYSLISPRVAFGKVLVLFLGLFCGASAGREGPTVQVGAAIMAAFGRLTPERLPGLLL
CCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCEEE
AGGAAGVAAAFNTPLAGIVFGIEELGRAYEARSSGLIVAGIVAAGLTSLGLLGNYTYFGT
ECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCEECC
TDAALPLAAGWIVPLVAVVGGLAGGLFSRLVILFARGLPGAAGRAIRKRPVIFAAACGLC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHH
VALCGLASGGAAYGTGYEEAKAILHGDAAAGWTYAPLKFAATVLSSISGIPGGLFAPSLA
HHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
VGAGLGGTVHGLLPGMPVGAVVLIGMVAYLTGVLQAPITSFVIVTEMTQNHAMVIPLMVA
HHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHH
ALLADAASKAICRGGLYHTLAEIMLERADVPAQPVAKTG
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MVEKRDEVRETTDAPPPQRSRYRRSIMRLRSASMEAYPLWRGRLLFLVGGIGVGLAAVLM
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AKGADLAQDGFRRLTAPSPLIALVLTPAGFALAALLALTVFPNSQGSGIPQVIAARHARD
HCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHC
AGFRYSLISPRVAFGKVLVLFLGLFCGASAGREGPTVQVGAAIMAAFGRLTPERLPGLLL
CCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCEEE
AGGAAGVAAAFNTPLAGIVFGIEELGRAYEARSSGLIVAGIVAAGLTSLGLLGNYTYFGT
ECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCEECC
TDAALPLAAGWIVPLVAVVGGLAGGLFSRLVILFARGLPGAAGRAIRKRPVIFAAACGLC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHH
VALCGLASGGAAYGTGYEEAKAILHGDAAAGWTYAPLKFAATVLSSISGIPGGLFAPSLA
HHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
VGAGLGGTVHGLLPGMPVGAVVLIGMVAYLTGVLQAPITSFVIVTEMTQNHAMVIPLMVA
HHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHH
ALLADAASKAICRGGLYHTLAEIMLERADVPAQPVAKTG
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA