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The map label for this gene is nusA [H]

Identifier: 170750127

GI number: 170750127

Start: 3970043

End: 3971680

Strand: Reverse

Name: nusA [H]

Synonym: Mrad2831_3729

Alternate gene names: 170750127

Gene position: 3971680-3970043 (Counterclockwise)

Preceding gene: 170750128

Following gene: 170750126

Centisome position: 65.35

GC content: 70.15

Gene sequence:

>1638_bases
ATGGCCGTCGTCAGCGCCAACAGGCTCGAACTCCTCCAGATCGCCGACGCGGTCGCCCGCGAGAAGGTGATCGACCGCTC
CATCGTGATCGACGCGATGGAGGAGGCGATCGCCAAGGCCGCCCGCTCCCGCTACGGCGCCGAGACCGACGTTCACGCCG
AGATCGACCCGAAGACCGGGGCGCTCCGCCTCTCGCGCCACCTCCTGGTCGTCGATCAGGTGGAGAACGACGCCCGCGAG
ATCACCCTCGACCAGGCCCGCCGCTACAACCCGGGCGCGCTGGTCGGCGACGTGATCTCCGACACCCTGCCGCCCTTCGA
TTTCGGCCGCGTCGCCGCCCAGTCGGCCAAGCAGGTCATCGTCCAGAAGGTCCGCGACGCCGAGCGCGCCCGCCAGTTCG
ACGAGTACAAGGACCGCATCGGCGAGATCCTCAACGGCATCGTGAAGCGCGTCGAGTACGGCAACGTCATCGTCGATCTC
GGCCGCGGCGAGGGCATCGTCCGCCGGGACGAGATGATCCCGCGCGAGACCTTCCGGCCGGGCGACCGCATCCGCTCCTA
CCTGTTCGACGTGCGCTCGGAAGTGCGCGGGCCGCAGATCTTCCTGTCGCGCTCGCACCCGCAGTTCATGGCCAAGCTGT
TCGGCCAGGAAGTGCCGGAGATCTACGACGGCATCGTCGAGGTGAAGGCGGTGGCCCGCGATCCGGGCTCGCGCGCCAAG
ATCGCGGTGATCTCCCGCGACGGCAGCATCGACCCCGTCGGCGCCTGCGTGGGCATGCGCGGCTCGCGCGTTCAGGCCGT
CGTCGGCGAGCTCCAGGGCGAGAAGATCGACATCATCCCGTGGTCGGAGGATGAGGCGACCTTCATCGTCAACGCCCTCC
AGCCCGCCGAGGTGGTGAAGGTGGTGCTCGACGAGGAGGCCGACCGCATCGAGGTGGTGGTGCCGGACGACCAGCTCTCG
CTGGCGATCGGCCGCCGGGGCCAGAACGTGCGCCTCGCCTCGCAGCTCACCGGCTGGGACATCGACATCCTGACTGAGGC
CGAGGAATCGGAGCGCCGCCAGAAGGAGTTCGCCGAGCGGACCCAGGTGTTCATGGAGGCGCTCGACGTCGACGAGACGG
TCGGCCAGCTGCTCGCGGCGGAAGGCTTCCGCAACGTCGAGGAGATCGCCTACGTCGACATCCAGGAGCTCTCCGGCATC
CAGGGGCTCGACGAGGAGACTGGGACCGAGATCCAGGCCCGCGCCCAGGACCACCTCGCCCGGATCGAGCAGGCGCACGA
CGCCCGCCGCACCGAGCTTGGCGTGTCCGACGAGCTGCGCGAGATCGAGGGCATCACCACGCCCATGATGGTCGCGCTCG
GCGAGAACGACGTGAAGACCGTCGAGGACTTGGCGGGCTGCGCCACCGACGACCTCGTCGGCTACACCGAGGGCCGCGGT
CCCGACGCCGTGCGCCACGCCGGCTACCTCGACGGGTTCGACTTGTCCCGCGCCGAGGCCGAGGCCCTGATCATGGCCGC
CCGCCTGAAGGCCGGCTGGATCGAGGCTCTGCCGGAGCCGGAGACCGAGGAAGGGGCCGAGGAGGGTGCCGAGGCGGCCG
AGGGCGAAGAGACCGCCCCCGCGCAGGCCGAGGCCTGA

Upstream 100 bases:

>100_bases
GCGTCCCGGCTCAAGGACCGCGACTCCCTGCACTGAGACCCCCGCGTCGGCTGCCCGGCGGCCGGCCCGAAGATTTGACG
GAAGAGAAGGACCGACACCG

Downstream 100 bases:

>100_bases
GCCGAGAGCCGGCGGAGGCGCGATGGTCGCGGTCGACACGACCCCGCCCGAGGACGGGCTGGAGGCCGGGGGGCTCGATG
CCGGCCCCGGCCGCCGCTTG

Product: NusA antitermination factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MAVVSANRLELLQIADAVAREKVIDRSIVIDAMEEAIAKAARSRYGAETDVHAEIDPKTGALRLSRHLLVVDQVENDARE
ITLDQARRYNPGALVGDVISDTLPPFDFGRVAAQSAKQVIVQKVRDAERARQFDEYKDRIGEILNGIVKRVEYGNVIVDL
GRGEGIVRRDEMIPRETFRPGDRIRSYLFDVRSEVRGPQIFLSRSHPQFMAKLFGQEVPEIYDGIVEVKAVARDPGSRAK
IAVISRDGSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSEDEATFIVNALQPAEVVKVVLDEEADRIEVVVPDDQLS
LAIGRRGQNVRLASQLTGWDIDILTEAEESERRQKEFAERTQVFMEALDVDETVGQLLAAEGFRNVEEIAYVDIQELSGI
QGLDEETGTEIQARAQDHLARIEQAHDARRTELGVSDELREIEGITTPMMVALGENDVKTVEDLAGCATDDLVGYTEGRG
PDAVRHAGYLDGFDLSRAEAEALIMAARLKAGWIEALPEPETEEGAEEGAEAAEGEETAPAQAEA

Sequences:

>Translated_545_residues
MAVVSANRLELLQIADAVAREKVIDRSIVIDAMEEAIAKAARSRYGAETDVHAEIDPKTGALRLSRHLLVVDQVENDARE
ITLDQARRYNPGALVGDVISDTLPPFDFGRVAAQSAKQVIVQKVRDAERARQFDEYKDRIGEILNGIVKRVEYGNVIVDL
GRGEGIVRRDEMIPRETFRPGDRIRSYLFDVRSEVRGPQIFLSRSHPQFMAKLFGQEVPEIYDGIVEVKAVARDPGSRAK
IAVISRDGSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSEDEATFIVNALQPAEVVKVVLDEEADRIEVVVPDDQLS
LAIGRRGQNVRLASQLTGWDIDILTEAEESERRQKEFAERTQVFMEALDVDETVGQLLAAEGFRNVEEIAYVDIQELSGI
QGLDEETGTEIQARAQDHLARIEQAHDARRTELGVSDELREIEGITTPMMVALGENDVKTVEDLAGCATDDLVGYTEGRG
PDAVRHAGYLDGFDLSRAEAEALIMAARLKAGWIEALPEPETEEGAEEGAEAAEGEETAPAQAEA
>Mature_544_residues
AVVSANRLELLQIADAVAREKVIDRSIVIDAMEEAIAKAARSRYGAETDVHAEIDPKTGALRLSRHLLVVDQVENDAREI
TLDQARRYNPGALVGDVISDTLPPFDFGRVAAQSAKQVIVQKVRDAERARQFDEYKDRIGEILNGIVKRVEYGNVIVDLG
RGEGIVRRDEMIPRETFRPGDRIRSYLFDVRSEVRGPQIFLSRSHPQFMAKLFGQEVPEIYDGIVEVKAVARDPGSRAKI
AVISRDGSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSEDEATFIVNALQPAEVVKVVLDEEADRIEVVVPDDQLSL
AIGRRGQNVRLASQLTGWDIDILTEAEESERRQKEFAERTQVFMEALDVDETVGQLLAAEGFRNVEEIAYVDIQELSGIQ
GLDEETGTEIQARAQDHLARIEQAHDARRTELGVSDELREIEGITTPMMVALGENDVKTVEDLAGCATDDLVGYTEGRGP
DAVRHAGYLDGFDLSRAEAEALIMAARLKAGWIEALPEPETEEGAEEGAEAAEGEETAPAQAEA

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=500, Percent_Identity=44.2, Blast_Score=406, Evalue=1e-114,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 59897; Mature: 59766

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVSANRLELLQIADAVAREKVIDRSIVIDAMEEAIAKAARSRYGAETDVHAEIDPKTG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
ALRLSRHLLVVDQVENDAREITLDQARRYNPGALVGDVISDTLPPFDFGRVAAQSAKQVI
HHHHHHEEEEEECCCCCHHHEEHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
VQKVRDAERARQFDEYKDRIGEILNGIVKRVEYGNVIVDLGRGEGIVRRDEMIPRETFRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEHHHCCCHHHCCC
GDRIRSYLFDVRSEVRGPQIFLSRSHPQFMAKLFGQEVPEIYDGIVEVKAVARDPGSRAK
HHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCE
IAVISRDGSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSEDEATFIVNALQPAEVVK
EEEEECCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCHHHHHH
VVLDEEADRIEVVVPDDQLSLAIGRRGQNVRLASQLTGWDIDILTEAEESERRQKEFAER
HHHCCCCCEEEEEECCCCEEEEECCCCCCEEEHHHCCCCCEEEECCCHHHHHHHHHHHHH
TQVFMEALDVDETVGQLLAAEGFRNVEEIAYVDIQELSGIQGLDEETGTEIQARAQDHLA
HHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHH
RIEQAHDARRTELGVSDELREIEGITTPMMVALGENDVKTVEDLAGCATDDLVGYTEGRG
HHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCC
PDAVRHAGYLDGFDLSRAEAEALIMAARLKAGWIEALPEPETEEGAEEGAEAAEGEETAP
CHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHCCCCCCCC
AQAEA
CCCCC
>Mature Secondary Structure 
AVVSANRLELLQIADAVAREKVIDRSIVIDAMEEAIAKAARSRYGAETDVHAEIDPKTG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
ALRLSRHLLVVDQVENDAREITLDQARRYNPGALVGDVISDTLPPFDFGRVAAQSAKQVI
HHHHHHEEEEEECCCCCHHHEEHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
VQKVRDAERARQFDEYKDRIGEILNGIVKRVEYGNVIVDLGRGEGIVRRDEMIPRETFRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEHHHCCCHHHCCC
GDRIRSYLFDVRSEVRGPQIFLSRSHPQFMAKLFGQEVPEIYDGIVEVKAVARDPGSRAK
HHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCE
IAVISRDGSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSEDEATFIVNALQPAEVVK
EEEEECCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCHHHHHH
VVLDEEADRIEVVVPDDQLSLAIGRRGQNVRLASQLTGWDIDILTEAEESERRQKEFAER
HHHCCCCCEEEEEECCCCEEEEECCCCCCEEEHHHCCCCCEEEECCCHHHHHHHHHHHHH
TQVFMEALDVDETVGQLLAAEGFRNVEEIAYVDIQELSGIQGLDEETGTEIQARAQDHLA
HHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHH
RIEQAHDARRTELGVSDELREIEGITTPMMVALGENDVKTVEDLAGCATDDLVGYTEGRG
HHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCC
PDAVRHAGYLDGFDLSRAEAEALIMAARLKAGWIEALPEPETEEGAEEGAEAAEGEETAP
CHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHCCCCCCCC
AQAEA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]