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The map label for this gene is mutM

Identifier: 170750114

GI number: 170750114

Start: 3954173

End: 3955063

Strand: Direct

Name: mutM

Synonym: Mrad2831_3716

Alternate gene names: 170750114

Gene position: 3954173-3955063 (Clockwise)

Preceding gene: 170750113

Following gene: 170750115

Centisome position: 65.06

GC content: 73.85

Gene sequence:

>891_bases
ATGCCGGAGCTTCCCGAGGTCGAGACCGTGCGGCGGGGATTGGCGCCCGCCCTGGTCGGCGCGCGCTTCAGCCGCGTCAC
CCTGCGCCGGGCCAACCTGCGCTTCCCCTTCCCCGAGCGCTTCGCCGCCCGCCTCGAGGGCCGGACCGTCACGGGCCTCG
CGCGCCGGGCGAAGTACCTCACCGCCGCGCTCGATTCCGGCGAGACGCTGGTCATGCATCTCGGCATGAGCGGCCGCTTC
GACGTGGCCCTGCCCGACGGCAGCAACCTGTCGCCGGGGGATTTCTACCTCGAGGGCGCCCAGGGCACGCCCAAGCACGA
CCACGTGGTGATGGCGATGAGCACCGGCGCCACGGTGACCTACAACGACGCCCGCCGCTTCGGCTTCATGGATCTGGTCC
CGAGCGCGGAGCTCGCGGCCTGCCGCCACTTCGCCCGGATGGGCGTGGAACCCCTCGACGGACTGACCGGGGCGGTGATC
GCGCGGCTCTTCCGGCACAAGATCGCGCCGCTGAAGGCCGCCCTGCTCGACCAGCGCCTGATCGCGGGCCTCGGCAACAT
CTACGTGTGCGAGGCGCTGCACCGGGCGCGCCTGCATCCGGAGGCCCCGGCCGGGAGCCTCGCCCGGCCGGACGGGCGCC
CGACGCCCGAGGCCAACGCCCTGGCCAAGGCGATCGTGCAGGTCCTCGAGGAGGCCGTGAAGGCCGGCGGCTCGACCCTG
CGCGACTACGCCCACACGGACGGCAGCGCGGGCGCCTTCCAGCACGCCTTCCGGGTCTACGACCGGGTCGGCCTGCCCTG
CAGCCGGCCGGGATGCGCCGGCGCGATCACCCGCATCGTGCAGGCCAACCGCTCGACCTTCTTCTGCGCCACCTGCCAGC
CCCCGGGCTGA

Upstream 100 bases:

>100_bases
GGCAAACCGCGCCGGTTCCGCGCGGTTGCGGCCCGGGCCGATCCCGCCGATATCCGGGGCCGGCGCACCGGGCGCCGCGC
ACGCGAAAGCCGCGAGAGAG

Downstream 100 bases:

>100_bases
GCGCGCCGGCCGGCGCCTATCGCGCCGCGGAACGGCAATTTCGCCACAAACCCGACTAGGGTTAGAGCCCTCGACGTGCG
CACCCGGTTGCGGGCAGCCG

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MPELPEVETVRRGLAPALVGARFSRVTLRRANLRFPFPERFAARLEGRTVTGLARRAKYLTAALDSGETLVMHLGMSGRF
DVALPDGSNLSPGDFYLEGAQGTPKHDHVVMAMSTGATVTYNDARRFGFMDLVPSAELAACRHFARMGVEPLDGLTGAVI
ARLFRHKIAPLKAALLDQRLIAGLGNIYVCEALHRARLHPEAPAGSLARPDGRPTPEANALAKAIVQVLEEAVKAGGSTL
RDYAHTDGSAGAFQHAFRVYDRVGLPCSRPGCAGAITRIVQANRSTFFCATCQPPG

Sequences:

>Translated_296_residues
MPELPEVETVRRGLAPALVGARFSRVTLRRANLRFPFPERFAARLEGRTVTGLARRAKYLTAALDSGETLVMHLGMSGRF
DVALPDGSNLSPGDFYLEGAQGTPKHDHVVMAMSTGATVTYNDARRFGFMDLVPSAELAACRHFARMGVEPLDGLTGAVI
ARLFRHKIAPLKAALLDQRLIAGLGNIYVCEALHRARLHPEAPAGSLARPDGRPTPEANALAKAIVQVLEEAVKAGGSTL
RDYAHTDGSAGAFQHAFRVYDRVGLPCSRPGCAGAITRIVQANRSTFFCATCQPPG
>Mature_295_residues
PELPEVETVRRGLAPALVGARFSRVTLRRANLRFPFPERFAARLEGRTVTGLARRAKYLTAALDSGETLVMHLGMSGRFD
VALPDGSNLSPGDFYLEGAQGTPKHDHVVMAMSTGATVTYNDARRFGFMDLVPSAELAACRHFARMGVEPLDGLTGAVIA
RLFRHKIAPLKAALLDQRLIAGLGNIYVCEALHRARLHPEAPAGSLARPDGRPTPEANALAKAIVQVLEEAVKAGGSTLR
DYAHTDGSAGAFQHAFRVYDRVGLPCSRPGCAGAITRIVQANRSTFFCATCQPPG

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=294, Percent_Identity=38.4353741496599, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_METRJ (B1LWY6)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001756374.1
- ProteinModelPortal:   B1LWY6
- SMR:   B1LWY6
- GeneID:   6139769
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_3716
- HOGENOM:   HBG690070
- OMA:   RSTFYCA
- ProtClustDB:   PRK01103
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 31755; Mature: 31624

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 284-284 BINDING 106-106 BINDING 125-125 BINDING 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETVRRGLAPALVGARFSRVTLRRANLRFPFPERFAARLEGRTVTGLARRAKYL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
TAALDSGETLVMHLGMSGRFDVALPDGSNLSPGDFYLEGAQGTPKHDHVVMAMSTGATVT
HHHHCCCCEEEEEECCCCCEEEECCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCEEE
YNDARRFGFMDLVPSAELAACRHFARMGVEPLDGLTGAVIARLFRHKIAPLKAALLDQRL
ECCHHHCCHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGLGNIYVCEALHRARLHPEAPAGSLARPDGRPTPEANALAKAIVQVLEEAVKAGGSTL
HHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
RDYAHTDGSAGAFQHAFRVYDRVGLPCSRPGCAGAITRIVQANRSTFFCATCQPPG
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCC
>Mature Secondary Structure 
PELPEVETVRRGLAPALVGARFSRVTLRRANLRFPFPERFAARLEGRTVTGLARRAKYL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
TAALDSGETLVMHLGMSGRFDVALPDGSNLSPGDFYLEGAQGTPKHDHVVMAMSTGATVT
HHHHCCCCEEEEEECCCCCEEEECCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCEEE
YNDARRFGFMDLVPSAELAACRHFARMGVEPLDGLTGAVIARLFRHKIAPLKAALLDQRL
ECCHHHCCHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGLGNIYVCEALHRARLHPEAPAGSLARPDGRPTPEANALAKAIVQVLEEAVKAGGSTL
HHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
RDYAHTDGSAGAFQHAFRVYDRVGLPCSRPGCAGAITRIVQANRSTFFCATCQPPG
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA