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The map label for this gene is cobT [H]

Identifier: 170750099

GI number: 170750099

Start: 3937443

End: 3939347

Strand: Direct

Name: cobT [H]

Synonym: Mrad2831_3700

Alternate gene names: 170750099

Gene position: 3937443-3939347 (Clockwise)

Preceding gene: 170750098

Following gene: 170750101

Centisome position: 64.78

GC content: 71.5

Gene sequence:

>1905_bases
ATGTCCATCTCCAACCGCAAACCCGGCGAGCGCCGCGAACCCGTGGCCGAGCCCCTGAAGCGCTCGGTGGCCGGGTGCCT
GCGCGCCATCGCCCGGCGCTCCGAGGTCGAGGTCACCTACGCCACCGACCGGCCGGCGCTGACCGGCGACAAGGCCCGCC
TGCCCGAGCCCCCGCGCAAGCTCTCGGCGCAGGACGTCGCGGTGCTGCGCGGCAACGCCGACGCCATGGCGCTGCGGCTC
GGCTGCCACGACGTCGCCGTCCACCGCCGCCTCGCCCCCGAGAACAGCGCCGCCCGGGCGGTCTATGACGCGGTGGAGCA
GGCGCGCGTGGAGGCGATCGGCTCGCGCCGGATGGCGGGCGTCGCCAGCAACATCTCGGCGATGCTGGAGGACCGCTATC
ACCGGGGCGGCAAGTACGAGACCATCACCGACCGGGCCGACGCCCCGATGGAGGACGCCGTCGCCCTGATGGTGCGCGAG
CGCCTGACCGGCCAGAAGCCGCCCGAGGCCGCCACCAAGATCGTCGATCTGTGGCGGGAGCACATCGAGGCCAAGGCCGG
CCCGAACCTCGACGGGCTGCTCGGCTCGATCGAGGACCAGCGCAAGTTCGCCCGCTCGGTGCGCGAGCTCCTCACCTCCC
TCGACATGTCGGACGAGACGCCGTTCGACGCCGAGGACGACGAGAACGACGACGAGAACGACGCCCAGGACGACGAGCAA
AACCCCAGCGAGGGCGATTCGGAGGAGAAGAGCCAGGGCGAGCGCGCCGAGATCGAGACCTCCGAGGAGGCCTCCGACGA
GATCGAGGAGGGCGCGCAGGAATCCGCCGACGCGCCGTCCGGTGAGTTGCCCGAGGAGGCCGAGGACGCGGAGTCGGAGG
AGGCGTCGGAGGCGTCGCGCCCGCCGCAGCGGCAGGGCAACGAGGCGCGCGGGCCCGAGTACAAGGTGTTCAATCCGCGG
TTCGACGAGGTCATCCACGCCGAGGAGCTGTGCGACGCCGACGAGCTGTCGCGCCTGCGCTCCTACCTCGACAAGCAGCT
CTCGCATCTGCAGGGCGTGGTCGGCCGGCTCGCGAACCGGCTGCAGCGGCGATTGCTCGCCCAGCAGAACCGCGCCTGGG
ATTTCGACCTGGAGGAGGGTCAGCTCGACCCAGCGCGGCTGGTCCGCGTGGTCATCGACCCCTACCAGCCGCTCTCCTTC
AAGCAGGAGAAGGACACGAATTTCCGCGACACGGTGGTGACGCTGCTCCTCGACAATTCGGGCTCCATGCGCGGCCGGCC
GATCACCGTGGCGGCGACCTGTGCCGACATCCTGGCGCGGACGCTGGAGCGCTGCGGCGTGAAGGTGGAGATCCTGGGCT
TCACCACCCGGGCCTGGAAGGGCGGCCAGAGCCGCGAGGCGTGGCTCGCCGCCGGCAAGCCGCCGAATCCCGGGCGCCTG
AACGACCTGCGCCACATCGTCTACAAGTCGGCCGACGCGCCCTGGCGGCGCGCGCGCAAGAACCTCGGGCTGATGATGCG
CGAGGGGCTGCTCAAGGAGAACATCGACGGCGAGGCGCTCGACTGGGCGCACAAGCGCCTGCTCGGGCGGCCGGAGCAGC
GCAAGATCCTGATGGTGATCTCCGACGGCGCGCCGGTGGACGACTCGACCCTGTCGGTGAATGCCGGCAACTACCTCGAG
CGGCACCTGCGCTGGATGATCGAGGAGATCGAGACGCGCTCGCCCGTGGAGCTCCTCGCCATCGGCATCGGCCACGACGT
GACCCGCTACTACCGCCGCGCCGTGACGATCATCGACGCCGAGGAGCTCGGCGGGGCGATGACCGAGAAGCTGGCCGAGC
TGTTCGAGGAGGACGGCGCGGCGCCGACCGGCCGCATGCTGCGCGCGGCGGGCGGGCGGCGCTGA

Upstream 100 bases:

>100_bases
AGATGAAGCGCTCGCCCAAAACCCTGCCCGCGGACTGTCCCTACAGCTGGGATGAGATCCTGAACCGCCCCTTCGCGTTC
GAGGACGACCGGTAGCGCTG

Downstream 100 bases:

>100_bases
TCGCCGGTCCGACCTAGCCGCTGCTCTGGCTCTCGGGCCGTCCGATCCGGGGCCTCATCCCGAGGCGCCCGAGCGCCGCG
ACTGACGCGTCCCTACCGTC

Product: cobalt chelatase, pCobT subunit

Products: NA

Alternate protein names: Hydrogenobyrinic acid a,c-diamide cobaltochelatase subunit CobT [H]

Number of amino acids: Translated: 634; Mature: 633

Protein sequence:

>634_residues
MSISNRKPGERREPVAEPLKRSVAGCLRAIARRSEVEVTYATDRPALTGDKARLPEPPRKLSAQDVAVLRGNADAMALRL
GCHDVAVHRRLAPENSAARAVYDAVEQARVEAIGSRRMAGVASNISAMLEDRYHRGGKYETITDRADAPMEDAVALMVRE
RLTGQKPPEAATKIVDLWREHIEAKAGPNLDGLLGSIEDQRKFARSVRELLTSLDMSDETPFDAEDDENDDENDAQDDEQ
NPSEGDSEEKSQGERAEIETSEEASDEIEEGAQESADAPSGELPEEAEDAESEEASEASRPPQRQGNEARGPEYKVFNPR
FDEVIHAEELCDADELSRLRSYLDKQLSHLQGVVGRLANRLQRRLLAQQNRAWDFDLEEGQLDPARLVRVVIDPYQPLSF
KQEKDTNFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPPNPGRL
NDLRHIVYKSADAPWRRARKNLGLMMREGLLKENIDGEALDWAHKRLLGRPEQRKILMVISDGAPVDDSTLSVNAGNYLE
RHLRWMIEEIETRSPVELLAIGIGHDVTRYYRRAVTIIDAEELGGAMTEKLAELFEEDGAAPTGRMLRAAGGRR

Sequences:

>Translated_634_residues
MSISNRKPGERREPVAEPLKRSVAGCLRAIARRSEVEVTYATDRPALTGDKARLPEPPRKLSAQDVAVLRGNADAMALRL
GCHDVAVHRRLAPENSAARAVYDAVEQARVEAIGSRRMAGVASNISAMLEDRYHRGGKYETITDRADAPMEDAVALMVRE
RLTGQKPPEAATKIVDLWREHIEAKAGPNLDGLLGSIEDQRKFARSVRELLTSLDMSDETPFDAEDDENDDENDAQDDEQ
NPSEGDSEEKSQGERAEIETSEEASDEIEEGAQESADAPSGELPEEAEDAESEEASEASRPPQRQGNEARGPEYKVFNPR
FDEVIHAEELCDADELSRLRSYLDKQLSHLQGVVGRLANRLQRRLLAQQNRAWDFDLEEGQLDPARLVRVVIDPYQPLSF
KQEKDTNFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPPNPGRL
NDLRHIVYKSADAPWRRARKNLGLMMREGLLKENIDGEALDWAHKRLLGRPEQRKILMVISDGAPVDDSTLSVNAGNYLE
RHLRWMIEEIETRSPVELLAIGIGHDVTRYYRRAVTIIDAEELGGAMTEKLAELFEEDGAAPTGRMLRAAGGRR
>Mature_633_residues
SISNRKPGERREPVAEPLKRSVAGCLRAIARRSEVEVTYATDRPALTGDKARLPEPPRKLSAQDVAVLRGNADAMALRLG
CHDVAVHRRLAPENSAARAVYDAVEQARVEAIGSRRMAGVASNISAMLEDRYHRGGKYETITDRADAPMEDAVALMVRER
LTGQKPPEAATKIVDLWREHIEAKAGPNLDGLLGSIEDQRKFARSVRELLTSLDMSDETPFDAEDDENDDENDAQDDEQN
PSEGDSEEKSQGERAEIETSEEASDEIEEGAQESADAPSGELPEEAEDAESEEASEASRPPQRQGNEARGPEYKVFNPRF
DEVIHAEELCDADELSRLRSYLDKQLSHLQGVVGRLANRLQRRLLAQQNRAWDFDLEEGQLDPARLVRVVIDPYQPLSFK
QEKDTNFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPPNPGRLN
DLRHIVYKSADAPWRRARKNLGLMMREGLLKENIDGEALDWAHKRLLGRPEQRKILMVISDGAPVDDSTLSVNAGNYLER
HLRWMIEEIETRSPVELLAIGIGHDVTRYYRRAVTIIDAEELGGAMTEKLAELFEEDGAAPTGRMLRAAGGRR

Specific function: Catalyzes cobalt insertion in the corrin ring [H]

COG id: COG4547

COG function: function code H; Cobalamin biosynthesis protein CobT (nicotinate-mononucleotide:5, 6-dimethylbenzimidazole phosphoribosyltransferase)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 VWFA domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006538
- InterPro:   IPR002035 [H]

Pfam domain/function: PF06213 CobT [H]

EC number: =6.6.1.2 [H]

Molecular weight: Translated: 70541; Mature: 70409

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50234 VWFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISNRKPGERREPVAEPLKRSVAGCLRAIARRSEVEVTYATDRPALTGDKARLPEPPRK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHH
LSAQDVAVLRGNADAMALRLGCHDVAVHRRLAPENSAARAVYDAVEQARVEAIGSRRMAG
CCCCCCCEEECCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VASNISAMLEDRYHRGGKYETITDRADAPMEDAVALMVRERLTGQKPPEAATKIVDLWRE
HHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
HIEAKAGPNLDGLLGSIEDQRKFARSVRELLTSLDMSDETPFDAEDDENDDENDAQDDEQ
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
NPSEGDSEEKSQGERAEIETSEEASDEIEEGAQESADAPSGELPEEAEDAESEEASEASR
CCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCHHHHHHHHCC
PPQRQGNEARGPEYKVFNPRFDEVIHAEELCDADELSRLRSYLDKQLSHLQGVVGRLANR
CCHHCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQRRLLAQQNRAWDFDLEEGQLDPARLVRVVIDPYQPLSFKQEKDTNFRDTVVTLLLDNS
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEECCC
GSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPPNPGRL
CCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCCEEECCCCCCCCHH
NDLRHIVYKSADAPWRRARKNLGLMMREGLLKENIDGEALDWAHKRLLGRPEQRKILMVI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEE
SDGAPVDDSTLSVNAGNYLERHLRWMIEEIETRSPVELLAIGIGHDVTRYYRRAVTIIDA
ECCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHEEECCCHHHHHHHHHHHHEEEH
EELGGAMTEKLAELFEEDGAAPTGRMLRAAGGRR
HHHCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
SISNRKPGERREPVAEPLKRSVAGCLRAIARRSEVEVTYATDRPALTGDKARLPEPPRK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHH
LSAQDVAVLRGNADAMALRLGCHDVAVHRRLAPENSAARAVYDAVEQARVEAIGSRRMAG
CCCCCCCEEECCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VASNISAMLEDRYHRGGKYETITDRADAPMEDAVALMVRERLTGQKPPEAATKIVDLWRE
HHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
HIEAKAGPNLDGLLGSIEDQRKFARSVRELLTSLDMSDETPFDAEDDENDDENDAQDDEQ
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
NPSEGDSEEKSQGERAEIETSEEASDEIEEGAQESADAPSGELPEEAEDAESEEASEASR
CCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCHHHHHHHHCC
PPQRQGNEARGPEYKVFNPRFDEVIHAEELCDADELSRLRSYLDKQLSHLQGVVGRLANR
CCHHCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQRRLLAQQNRAWDFDLEEGQLDPARLVRVVIDPYQPLSFKQEKDTNFRDTVVTLLLDNS
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEECCC
GSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPPNPGRL
CCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCCEEECCCCCCCCHH
NDLRHIVYKSADAPWRRARKNLGLMMREGLLKENIDGEALDWAHKRLLGRPEQRKILMVI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEE
SDGAPVDDSTLSVNAGNYLERHLRWMIEEIETRSPVELLAIGIGHDVTRYYRRAVTIIDA
ECCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHEEECCCHHHHHHHHHHHHEEEH
EELGGAMTEKLAELFEEDGAAPTGRMLRAAGGRR
HHHCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1917840 [H]