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The map label for this gene is rnhB [H]

Identifier: 170750072

GI number: 170750072

Start: 3905228

End: 3905926

Strand: Direct

Name: rnhB [H]

Synonym: Mrad2831_3673

Alternate gene names: 170750072

Gene position: 3905228-3905926 (Clockwise)

Preceding gene: 170750071

Following gene: 170750075

Centisome position: 64.25

GC content: 76.97

Gene sequence:

>699_bases
ATGCCGTCCGCCAAATCGTTCGCCAAGCCGCACGCCAAGCCGCACGCCGGACCGCCCACTGGATCGACCGCCGGACCGCG
CGCGTCCGTCAGGACCCGGTCCCCGGGCGCTGGCCCGTTCGATCCCGCCCGGGCCGCCGCGTATCCGGCGGCGATCGGCT
GCGACGAGGTCGGGCGCGGCGCGCTCTGCGGACCGGTGGTGGTGGCCGCGGTCTGGTTCGATCCGACGGCGATCCCGCCC
GACCTGCTCGCGGCCCTCGACGACAGCAAGCGCGTTCCCGAGCCGGTGCGGGAGCGGCTGGCGCCCCTGATCCGCGCCCA
CGCGCGGGTCGCGGTGGCGGCGGGATCGCGCGCCCTGATCGACCGGATCAACATCCGCGGCGCGACCCTCGACGCCATGG
CGCGGGCGGTGGCGCGACTCGGCCTCGCCGCGCCCGTGCTGGTCGACGGGCGCGACGCCCTGCCGGGCCTGCCCGGCCGC
GCCGTGATCGGCGGCGACCACCTCGTGCCGCAGATCGCCGCCGCCTCGATCGTCGCCAAGGTGCTCCGGGACCAGCTGAT
GCGGCGCCTCGCCCGGCGCCATCCCGGCTACGGCTGGGAGCGGAACGTCGGCTACGGGACGGCCGTCCACCGCGCCGGCC
TGACGCAGCTCGGGCGCAGCGCGCATCACCGGCTCAGCTTCACCCGCGGGTTCGACTGA

Upstream 100 bases:

>100_bases
AGTTCTTCGGCAACATCGTCGAGGCGCATGGCGGTGCCCGGAGCACCCGGGACGCCGCCTGACGCGTGACGGCGCCCGCG
CGGGATGGCAGGACGGCGCG

Downstream 100 bases:

>100_bases
GCCGGGCAAGACGGATCACGCGGCCCGGACGCCCGCCAGGAAGCGGTGGACCTCCGCGGCGAGTTCCTCCGACTGACGCG
TGAGCGCGGTGGCGGCCGTG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MPSAKSFAKPHAKPHAGPPTGSTAGPRASVRTRSPGAGPFDPARAAAYPAAIGCDEVGRGALCGPVVVAAVWFDPTAIPP
DLLAALDDSKRVPEPVRERLAPLIRAHARVAVAAGSRALIDRINIRGATLDAMARAVARLGLAAPVLVDGRDALPGLPGR
AVIGGDHLVPQIAAASIVAKVLRDQLMRRLARRHPGYGWERNVGYGTAVHRAGLTQLGRSAHHRLSFTRGFD

Sequences:

>Translated_232_residues
MPSAKSFAKPHAKPHAGPPTGSTAGPRASVRTRSPGAGPFDPARAAAYPAAIGCDEVGRGALCGPVVVAAVWFDPTAIPP
DLLAALDDSKRVPEPVRERLAPLIRAHARVAVAAGSRALIDRINIRGATLDAMARAVARLGLAAPVLVDGRDALPGLPGR
AVIGGDHLVPQIAAASIVAKVLRDQLMRRLARRHPGYGWERNVGYGTAVHRAGLTQLGRSAHHRLSFTRGFD
>Mature_231_residues
PSAKSFAKPHAKPHAGPPTGSTAGPRASVRTRSPGAGPFDPARAAAYPAAIGCDEVGRGALCGPVVVAAVWFDPTAIPPD
LLAALDDSKRVPEPVRERLAPLIRAHARVAVAAGSRALIDRINIRGATLDAMARAVARLGLAAPVLVDGRDALPGLPGRA
VIGGDHLVPQIAAASIVAKVLRDQLMRRLARRHPGYGWERNVGYGTAVHRAGLTQLGRSAHHRLSFTRGFD

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=179, Percent_Identity=45.8100558659218, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 24203; Mature: 24072

Theoretical pI: Translated: 11.80; Mature: 11.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSAKSFAKPHAKPHAGPPTGSTAGPRASVRTRSPGAGPFDPARAAAYPAAIGCDEVGRG
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHCCCC
ALCGPVVVAAVWFDPTAIPPDLLAALDDSKRVPEPVRERLAPLIRAHARVAVAAGSRALI
CHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
DRINIRGATLDAMARAVARLGLAAPVLVDGRDALPGLPGRAVIGGDHLVPQIAAASIVAK
HHHCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHH
VLRDQLMRRLARRHPGYGWERNVGYGTAVHRAGLTQLGRSAHHRLSFTRGFD
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHCCCC
>Mature Secondary Structure 
PSAKSFAKPHAKPHAGPPTGSTAGPRASVRTRSPGAGPFDPARAAAYPAAIGCDEVGRG
CCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHCCCC
ALCGPVVVAAVWFDPTAIPPDLLAALDDSKRVPEPVRERLAPLIRAHARVAVAAGSRALI
CHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
DRINIRGATLDAMARAVARLGLAAPVLVDGRDALPGLPGRAVIGGDHLVPQIAAASIVAK
HHHCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHH
VLRDQLMRRLARRHPGYGWERNVGYGTAVHRAGLTQLGRSAHHRLSFTRGFD
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499342 [H]