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The map label for this gene is kdsB

Identifier: 170750048

GI number: 170750048

Start: 3883219

End: 3883962

Strand: Direct

Name: kdsB

Synonym: Mrad2831_3649

Alternate gene names: 170750048

Gene position: 3883219-3883962 (Clockwise)

Preceding gene: 170750045

Following gene: 170750049

Centisome position: 63.89

GC content: 73.52

Gene sequence:

>744_bases
ATGTCCGATCCCCTGGTCCTGATCCCGGCGCGCCTCGCCGCGACCCGCCTGCCGAACAAGCCGCTGGCCGACATCGCCGG
CGAGGCCATGATCGTCCATGTCTGGCGCCGGGCCGTGGAGGCCGGCATCGGCCCCGTGGTGGTCGCCACCGACACGGCGG
AGATCGCCCGCACGGTCGAGGCCGCGGGCGGCCTCGCGGTGATGACCCGGGCCGACCACCCATCGGGTTCCGACCGGCTC
GCCGAGGCGCTGGAGATCGTCGATCCGGAGGGGCGCCACGACGTGGTCGTCAACGTGCAGGGCGACCTGCCGACCATCGA
CCCGGCGATCATCGGCGCCGCTATCGTGCCGCTCGCCGACCGCACCGTCGACATCGTCACCCTGTGCGCGCCGATCACCG
ATCCGCACGAGGCCGACAATCCCAACGTGGTCAAGCTCGTCGGCCACGCGGTCGGTCCCGGGCGACTGCGCGCCCTCTAC
TTCACCCGCGCCCGCGCGCCCTGGGGCGACGGGCCCCTCTGGCACCATATCGGCCTGTACGCCTACCGGCGCACGGCGCT
GAGCCGATTCGTGGCCCTGCCGCCCGGCGAGCTGGAGGTGCGGGAGAAGCTGGAGCAGCTGCGGGCCCTCGAGGCCGGCA
TGCGCATCGATGCCGCCATCGTCGACGACCTGCCGCTCGGCGTCGACACTCCCGCCGACCTGGAACGGGCCCGCGCCCTG
CTGCAGGGCCGGCGCCTGAACTGA

Upstream 100 bases:

>100_bases
CCGGCCCGTTTCCGGGGACGACAACCGCGGGCCGGCCTGTTAAGCAGCCGGCCGCCTCCCGCCGCGGCCGCTCGACCCGC
GCATCCGGATCCGAGTCTCG

Downstream 100 bases:

>100_bases
CACGCCGAACGAATCCGCGCCGCCATGACCGACCGCACCATCGCCTACCAGGGTGAGCCCGGGGCCAACTCGCACATCAT
CTGCGCGGAGGCCTACCCGG

Product: 3-deoxy-D-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSDPLVLIPARLAATRLPNKPLADIAGEAMIVHVWRRAVEAGIGPVVVATDTAEIARTVEAAGGLAVMTRADHPSGSDRL
AEALEIVDPEGRHDVVVNVQGDLPTIDPAIIGAAIVPLADRTVDIVTLCAPITDPHEADNPNVVKLVGHAVGPGRLRALY
FTRARAPWGDGPLWHHIGLYAYRRTALSRFVALPPGELEVREKLEQLRALEAGMRIDAAIVDDLPLGVDTPADLERARAL
LQGRRLN

Sequences:

>Translated_247_residues
MSDPLVLIPARLAATRLPNKPLADIAGEAMIVHVWRRAVEAGIGPVVVATDTAEIARTVEAAGGLAVMTRADHPSGSDRL
AEALEIVDPEGRHDVVVNVQGDLPTIDPAIIGAAIVPLADRTVDIVTLCAPITDPHEADNPNVVKLVGHAVGPGRLRALY
FTRARAPWGDGPLWHHIGLYAYRRTALSRFVALPPGELEVREKLEQLRALEAGMRIDAAIVDDLPLGVDTPADLERARAL
LQGRRLN
>Mature_246_residues
SDPLVLIPARLAATRLPNKPLADIAGEAMIVHVWRRAVEAGIGPVVVATDTAEIARTVEAAGGLAVMTRADHPSGSDRLA
EALEIVDPEGRHDVVVNVQGDLPTIDPAIIGAAIVPLADRTVDIVTLCAPITDPHEADNPNVVKLVGHAVGPGRLRALYF
TRARAPWGDGPLWHHIGLYAYRRTALSRFVALPPGELEVREKLEQLRALEAGMRIDAAIVDDLPLGVDTPADLERARALL
QGRRLN

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=251, Percent_Identity=48.605577689243, Blast_Score=194, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_METRJ (B1LVX5)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001756308.1
- ProteinModelPortal:   B1LVX5
- SMR:   B1LVX5
- GeneID:   6139702
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_3649
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- ProtClustDB:   CLSK2330202
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 26437; Mature: 26306

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPLVLIPARLAATRLPNKPLADIAGEAMIVHVWRRAVEAGIGPVVVATDTAEIARTVE
CCCCEEEECCHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHH
AAGGLAVMTRADHPSGSDRLAEALEIVDPEGRHDVVVNVQGDLPTIDPAIIGAAIVPLAD
HCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCC
RTVDIVTLCAPITDPHEADNPNVVKLVGHAVGPGRLRALYFTRARAPWGDGPLWHHIGLY
CCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCHHHHHHHH
AYRRTALSRFVALPPGELEVREKLEQLRALEAGMRIDAAIVDDLPLGVDTPADLERARAL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEECEEEECCCCCCCCCCHHHHHHHHH
LQGRRLN
HCCCCCC
>Mature Secondary Structure 
SDPLVLIPARLAATRLPNKPLADIAGEAMIVHVWRRAVEAGIGPVVVATDTAEIARTVE
CCCEEEECCHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHH
AAGGLAVMTRADHPSGSDRLAEALEIVDPEGRHDVVVNVQGDLPTIDPAIIGAAIVPLAD
HCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCC
RTVDIVTLCAPITDPHEADNPNVVKLVGHAVGPGRLRALYFTRARAPWGDGPLWHHIGLY
CCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCHHHHHHHH
AYRRTALSRFVALPPGELEVREKLEQLRALEAGMRIDAAIVDDLPLGVDTPADLERARAL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEECEEEECCCCCCCCCCHHHHHHHHH
LQGRRLN
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA