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The map label for this gene is hmp [H]

Identifier: 170750042

GI number: 170750042

Start: 3876600

End: 3877841

Strand: Direct

Name: hmp [H]

Synonym: Mrad2831_3643

Alternate gene names: 170750042

Gene position: 3876600-3877841 (Clockwise)

Preceding gene: 170750040

Following gene: 170750044

Centisome position: 63.78

GC content: 72.54

Gene sequence:

>1242_bases
ATGCCCGCGCCCCTGTCCCCCGCCACCGTCGCTCTCATCAAGGCCACCGTCCCGGCCCTGGAGGCCCACGGCCTCACCAT
CACGCGGCGCATGTACGAGCGCCTGTTCGAGAACGCGGAGATCCGCGACCTCTTCAACCAGTCCCATCACGGCGAGACCG
GCGCGCAGCCGAGGGCCCTGGCCCAGGCCGTGCTCGCCTACGCCCGCAACATCGACAATCTCGGTGTGCTGGCCGGAGCG
GTGGAGCGTATCGCGCAGAAGCACGTCGCCCTGAACATCCTGCCGGAGCACTATCCCCACGTCGCCGACGCGCTCCTCGG
CGCGATCGGGGACGTCCTCGGTGAGGCCGCCACGCCGGAGATCGCCGCGGCCTGGGGGGAGGCGTACTGGTTCCTGGCGG
AACTGCTGATCGGCCGGGAGGCCGCGATCTACCGCGACCACGCCTCCGAGCCGGGCGGCTGGAACGGCTGGCGCGACTTC
GTGGTCGAGAGCGTGACCCAGGAGAGCGAGACGATCCGCTCCTTCGTCCTGGTCCCAGCCGATGGCGGCCCGGTGCTGCG
GCATCGGCCGGGCCAGTATCTCGGCTTCCTCCTGGACCTGCCGGGCCGCGGCGTGCTGAAGCGGAACTACTCGGTGTCCT
GCGCGCCGAGCGACCGGGCCTACCGCATCACCGTCAAGCGCGAGGCGGCGCCGGGCCGGCCGGCCGGGATCGCCTCGAAC
TGGCTGCACGACCACGCCCAGCCCGGCACCGTGCTGAAGGTCGCGGCTCCGGCGGGCGATTTCAGCCTCGACGCGGAGGG
CGACGCGCCCGTGGTGCTGGTCAGCGGCGGCGTCGGGCTGACCCCGATGATCAGCATGCTCGAGACCATCGCGGCGGACA
GCCCGGACCGGCCGACCTGGTACGTGCACGGCGCCCTGTTCGGCCGCGTGCACGCCCTGCGCGACCACGCCCGGGCGCTC
GCGGCGCGGGCCCGCAACGCGCGGCTCGTGACCTTCTACGCGGAGCCCGAAGCCGCGGACCGGCCGGGTGAGCACTACGA
CGTCAAGGGCCTGATCACGGCGGACTGGCTCGTCGAGCACACGCCGCACGACCGAGCGACCTACTATCTGTGCGGTCCCA
AGCCGTTCCTTCGGGATCTCGCCACCGGCCTGCTGCGCCGCGGCGTCCCGGCCGAGCGGGTGAAGTTCGAGTTCTTCGGC
CCGGCCGACGAGCTGTTCGACGAGCCCCGGCAGGCGGCCTAG

Upstream 100 bases:

>100_bases
GACGCGGGGCCGAGCGCCGCCCCATAAGATATATTTCGATTGCACCTTTTCGAGGGGCGCGCTAGAAGATGCATGTCGTA
TGCACCTTCTGAGCCGTGCC

Downstream 100 bases:

>100_bases
GCGGCGCTCAAGCCGGCCCGGCGCCGCGGAGCGGGCGCTCCGGGCCGAGCACCCGCGCCACGGTCGTCCGCAGGGCCTGC
GCGCTTCGGTAGCCCACCGC

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MPAPLSPATVALIKATVPALEAHGLTITRRMYERLFENAEIRDLFNQSHHGETGAQPRALAQAVLAYARNIDNLGVLAGA
VERIAQKHVALNILPEHYPHVADALLGAIGDVLGEAATPEIAAAWGEAYWFLAELLIGREAAIYRDHASEPGGWNGWRDF
VVESVTQESETIRSFVLVPADGGPVLRHRPGQYLGFLLDLPGRGVLKRNYSVSCAPSDRAYRITVKREAAPGRPAGIASN
WLHDHAQPGTVLKVAAPAGDFSLDAEGDAPVVLVSGGVGLTPMISMLETIAADSPDRPTWYVHGALFGRVHALRDHARAL
AARARNARLVTFYAEPEAADRPGEHYDVKGLITADWLVEHTPHDRATYYLCGPKPFLRDLATGLLRRGVPAERVKFEFFG
PADELFDEPRQAA

Sequences:

>Translated_413_residues
MPAPLSPATVALIKATVPALEAHGLTITRRMYERLFENAEIRDLFNQSHHGETGAQPRALAQAVLAYARNIDNLGVLAGA
VERIAQKHVALNILPEHYPHVADALLGAIGDVLGEAATPEIAAAWGEAYWFLAELLIGREAAIYRDHASEPGGWNGWRDF
VVESVTQESETIRSFVLVPADGGPVLRHRPGQYLGFLLDLPGRGVLKRNYSVSCAPSDRAYRITVKREAAPGRPAGIASN
WLHDHAQPGTVLKVAAPAGDFSLDAEGDAPVVLVSGGVGLTPMISMLETIAADSPDRPTWYVHGALFGRVHALRDHARAL
AARARNARLVTFYAEPEAADRPGEHYDVKGLITADWLVEHTPHDRATYYLCGPKPFLRDLATGLLRRGVPAERVKFEFFG
PADELFDEPRQAA
>Mature_412_residues
PAPLSPATVALIKATVPALEAHGLTITRRMYERLFENAEIRDLFNQSHHGETGAQPRALAQAVLAYARNIDNLGVLAGAV
ERIAQKHVALNILPEHYPHVADALLGAIGDVLGEAATPEIAAAWGEAYWFLAELLIGREAAIYRDHASEPGGWNGWRDFV
VESVTQESETIRSFVLVPADGGPVLRHRPGQYLGFLLDLPGRGVLKRNYSVSCAPSDRAYRITVKREAAPGRPAGIASNW
LHDHAQPGTVLKVAAPAGDFSLDAEGDAPVVLVSGGVGLTPMISMLETIAADSPDRPTWYVHGALFGRVHALRDHARALA
ARARNARLVTFYAEPEAADRPGEHYDVKGLITADWLVEHTPHDRATYYLCGPKPFLRDLATGLLRRGVPAERVKFEFFGP
ADELFDEPRQAA

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=401, Percent_Identity=41.1471321695761, Blast_Score=285, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6321673, Length=411, Percent_Identity=37.2262773722628, Blast_Score=229, Evalue=5e-61,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 44871; Mature: 44740

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPLSPATVALIKATVPALEAHGLTITRRMYERLFENAEIRDLFNQSHHGETGAQPRAL
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHH
AQAVLAYARNIDNLGVLAGAVERIAQKHVALNILPEHYPHVADALLGAIGDVLGEAATPE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCHHCHHHHHHHHHHHHHHHCCCCCCH
IAAAWGEAYWFLAELLIGREAAIYRDHASEPGGWNGWRDFVVESVTQESETIRSFVLVPA
HHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEC
DGGPVLRHRPGQYLGFLLDLPGRGVLKRNYSVSCAPSDRAYRITVKREAAPGRPAGIASN
CCCCCCCCCCCHHEEEEEECCCCCEEECCCEEEECCCCCEEEEEEEECCCCCCCCCHHHH
WLHDHAQPGTVLKVAAPAGDFSLDAEGDAPVVLVSGGVGLTPMISMLETIAADSPDRPTW
HHHHCCCCCCEEEEECCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEE
YVHGALFGRVHALRDHARALAARARNARLVTFYAEPEAADRPGEHYDVKGLITADWLVEH
EEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEHHHEEC
TPHDRATYYLCGPKPFLRDLATGLLRRGVPAERVKFEFFGPADELFDEPRQAA
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHEEEEEECCHHHHHCCCCCCC
>Mature Secondary Structure 
PAPLSPATVALIKATVPALEAHGLTITRRMYERLFENAEIRDLFNQSHHGETGAQPRAL
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHH
AQAVLAYARNIDNLGVLAGAVERIAQKHVALNILPEHYPHVADALLGAIGDVLGEAATPE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCHHCHHHHHHHHHHHHHHHCCCCCCH
IAAAWGEAYWFLAELLIGREAAIYRDHASEPGGWNGWRDFVVESVTQESETIRSFVLVPA
HHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEC
DGGPVLRHRPGQYLGFLLDLPGRGVLKRNYSVSCAPSDRAYRITVKREAAPGRPAGIASN
CCCCCCCCCCCHHEEEEEECCCCCEEECCCEEEECCCCCEEEEEEEECCCCCCCCCHHHH
WLHDHAQPGTVLKVAAPAGDFSLDAEGDAPVVLVSGGVGLTPMISMLETIAADSPDRPTW
HHHHCCCCCCEEEEECCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEE
YVHGALFGRVHALRDHARALAARARNARLVTFYAEPEAADRPGEHYDVKGLITADWLVEH
EEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEHHHEEC
TPHDRATYYLCGPKPFLRDLATGLLRRGVPAERVKFEFFGPADELFDEPRQAA
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHEEEEEECCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]