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The map label for this gene is afr [H]

Identifier: 170749994

GI number: 170749994

Start: 3821996

End: 3822991

Strand: Reverse

Name: afr [H]

Synonym: Mrad2831_3595

Alternate gene names: 170749994

Gene position: 3822991-3821996 (Counterclockwise)

Preceding gene: 170749995

Following gene: 170749993

Centisome position: 62.9

GC content: 75.2

Gene sequence:

>996_bases
ATGGACGCGGTCGGCTGGGGCATCGTCGGCTACGGCTGGGTCGCCCGCGACTACATGGAGCCGGGCATCCGCGCCGCCGG
GCACCGGCTGGTCGCCGTCTGCGATCCGAACCCGGAGAGCCGAGCCGCAGCCGAGCGAGCGGGCGCGCGCGTCCATGCCG
ACCTCGCCGGCCTCATCGCCGAGCCCGCGGTCGAGGCGGTCTACGTGGCGACGCCGAACCACCTCCACCGCGGCGCCGTG
GAAGCGCTGGCGGCGGCCGGCAAGGCGGTGCTCTGCGAGAAGCCCATGGCGGCGACGCTCGGGGACGCCGAGGCCATTGC
TCACGCCGTCGAGTCGCAAAGAATCTTCTACGGCACGGCCTTCGACCAGCGGCACCACCCGGCCCACCGGGCGATCCGCG
CGCAGGTCGAGGCCGGGCGGCTCGGCACCGTGACGGCGGTGCGGATCGTCTATGCCTGCTGGCTCGGGCGCGACTGGTCG
GAGGCCGGCCAGCCGAACTGGCGGATCGACGCCGCCCAGGCCGGAGGCGGCGCGCTGATGGACCTCGCCCCGCACGGGCT
CGACCTCGTGGACTTCCTGCTCGGCGAGCCGATCGCCGACCTCGCGGCCCTGACGCAGACCAGGGCCCAGGACTACGCGG
TCGACGACGGCGCCCTGCTGATCGGCCGGACCGCGGGCGGCGCGCTGGCGAGCCTCCACGTCGCCTACAATTGCCCGGAC
GCGCTGCCCCGCCGCCGCCTGGAGGTGGTCGGCACGAAGGGCCTGCTCGTCGCCGAGAACACGATGGGCCAGACGGCCGG
CGGATCCCTGACCTTCATCGACGGCGCCAGCGGCCGCGCGGAGCCGCTCGCCTTCGACGCCGACAGCTCGCCCTTCGCCG
AGCAGGTGCGCGCCTTCGGCTCGGCCCTGCGCCGGCCCGAGGAGCGAGTGGCCTACTCGGCCGCGCGCGACCTCCACACG
ATGCGGCTCGTCGCGCGCGCGTACGGGCAGGGGTAG

Upstream 100 bases:

>100_bases
CCCCGGGCCCCGAGTCGCTGACCTTCGACGAGAGCCTCGATGCGCCGGAGACGCAGCGCGTGCGCGTGCGCGAATTCGTC
GACCTGCCGGAGCGGTGGTG

Downstream 100 bases:

>100_bases
CGGACAGGAATTTCAATCACGCAACCGACCCAAGCATCTCCCACTCACCCCTCATCCTGAGGTGCCGGAGCGCAGTGGAG
GCCTCGAAGGAGGGCTCCAG

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MDAVGWGIVGYGWVARDYMEPGIRAAGHRLVAVCDPNPESRAAAERAGARVHADLAGLIAEPAVEAVYVATPNHLHRGAV
EALAAAGKAVLCEKPMAATLGDAEAIAHAVESQRIFYGTAFDQRHHPAHRAIRAQVEAGRLGTVTAVRIVYACWLGRDWS
EAGQPNWRIDAAQAGGGALMDLAPHGLDLVDFLLGEPIADLAALTQTRAQDYAVDDGALLIGRTAGGALASLHVAYNCPD
ALPRRRLEVVGTKGLLVAENTMGQTAGGSLTFIDGASGRAEPLAFDADSSPFAEQVRAFGSALRRPEERVAYSAARDLHT
MRLVARAYGQG

Sequences:

>Translated_331_residues
MDAVGWGIVGYGWVARDYMEPGIRAAGHRLVAVCDPNPESRAAAERAGARVHADLAGLIAEPAVEAVYVATPNHLHRGAV
EALAAAGKAVLCEKPMAATLGDAEAIAHAVESQRIFYGTAFDQRHHPAHRAIRAQVEAGRLGTVTAVRIVYACWLGRDWS
EAGQPNWRIDAAQAGGGALMDLAPHGLDLVDFLLGEPIADLAALTQTRAQDYAVDDGALLIGRTAGGALASLHVAYNCPD
ALPRRRLEVVGTKGLLVAENTMGQTAGGSLTFIDGASGRAEPLAFDADSSPFAEQVRAFGSALRRPEERVAYSAARDLHT
MRLVARAYGQG
>Mature_331_residues
MDAVGWGIVGYGWVARDYMEPGIRAAGHRLVAVCDPNPESRAAAERAGARVHADLAGLIAEPAVEAVYVATPNHLHRGAV
EALAAAGKAVLCEKPMAATLGDAEAIAHAVESQRIFYGTAFDQRHHPAHRAIRAQVEAGRLGTVTAVRIVYACWLGRDWS
EAGQPNWRIDAAQAGGGALMDLAPHGLDLVDFLLGEPIADLAALTQTRAQDYAVDDGALLIGRTAGGALASLHVAYNCPD
ALPRRRLEVVGTKGLLVAENTMGQTAGGSLTFIDGASGRAEPLAFDADSSPFAEQVRAFGSALRRPEERVAYSAARDLHT
MRLVARAYGQG

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=263, Percent_Identity=26.2357414448669, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI87081947, Length=210, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693182, Length=198, Percent_Identity=26.2626262626263, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581115, Length=236, Percent_Identity=27.1186440677966, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 34840; Mature: 34840

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVGWGIVGYGWVARDYMEPGIRAAGHRLVAVCDPNPESRAAAERAGARVHADLAGLIA
CCCCCCCHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCHHHHHHHHCCCEEHHHHHHHHH
EPAVEAVYVATPNHLHRGAVEALAAAGKAVLCEKPMAATLGDAEAIAHAVESQRIFYGTA
CCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCHHHCCHHHHHHHHHHCCEEEEEEC
FDQRHHPAHRAIRAQVEAGRLGTVTAVRIVYACWLGRDWSEAGQPNWRIDAAQAGGGALM
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCCEEE
DLAPHGLDLVDFLLGEPIADLAALTQTRAQDYAVDDGALLIGRTAGGALASLHVAYNCPD
ECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCEECCCCEEEEECCCCCEEEEEEEECCCC
ALPRRRLEVVGTKGLLVAENTMGQTAGGSLTFIDGASGRAEPLAFDADSSPFAEQVRAFG
HHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCEEECCCCCHHHHHHHHHH
SALRRPEERVAYSAARDLHTMRLVARAYGQG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDAVGWGIVGYGWVARDYMEPGIRAAGHRLVAVCDPNPESRAAAERAGARVHADLAGLIA
CCCCCCCHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCHHHHHHHHCCCEEHHHHHHHHH
EPAVEAVYVATPNHLHRGAVEALAAAGKAVLCEKPMAATLGDAEAIAHAVESQRIFYGTA
CCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCHHHCCHHHHHHHHHHCCEEEEEEC
FDQRHHPAHRAIRAQVEAGRLGTVTAVRIVYACWLGRDWSEAGQPNWRIDAAQAGGGALM
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCCEEE
DLAPHGLDLVDFLLGEPIADLAALTQTRAQDYAVDDGALLIGRTAGGALASLHVAYNCPD
ECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCEECCCCEEEEECCCCCEEEEEEEECCCC
ALPRRRLEVVGTKGLLVAENTMGQTAGGSLTFIDGASGRAEPLAFDADSSPFAEQVRAFG
HHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCEEECCCCCHHHHHHHHHH
SALRRPEERVAYSAARDLHTMRLVARAYGQG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA