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The map label for this gene is thcD [H]

Identifier: 170749960

GI number: 170749960

Start: 3779481

End: 3780719

Strand: Direct

Name: thcD [H]

Synonym: Mrad2831_3561

Alternate gene names: 170749960

Gene position: 3779481-3780719 (Clockwise)

Preceding gene: 170749959

Following gene: 170749964

Centisome position: 62.18

GC content: 76.27

Gene sequence:

>1239_bases
GTGAGCGCCGGCGTCGTCGTGGCCGGGGCCGGGCAGGCCGGCTTCCAGCTCGCCGCCTCCTTGCGGGAGGGCGGGTTCCG
GGACCCGATCACCCTGGTGGGCGACGAGGCCGCCCTGCCCTACCAGCGGCCGCCCCTCTCGAAGGCCTACCTCGCCGGCA
AGACCGACCAGGAGGGCCTGTTCCTGCGCCCGCCCGGCTTCTTCGCCGAGCACGCCGTCGCGCACCGTCCGGGGCTCCGC
GCCGTCGCGATCGACCGGGATGCGCGCCGCCTGCAGCTCTCGGACGGCACCGACCTCCCTTACGACCACCTCGTGCTCGC
CACCGGCGCGCGCAACCGCCCCCTGCCGGTCCCGGGCGCCGAACTCGCGGGCGTGCGCCAGCTGCGCGGCCTCGCGGATG
CCGACGCCCTCAAGGAATCGCTCGCGACGGCCCGGGCCATCGTGGTCGTCGGGGCCGGCTTCATCGGGCTCGAATTCGCC
GCCGTCGCGGCCGCGCGCGGCCTCCCGGTGACGGTGATCGAGGCGGCCGAGCGGCCCATGGCCCGGGCGGTCTCGTCCGA
GATGGGCGCGTTCTTCCGCCGCGCCCACGCGGCGATGGGCGTGCGCTTCGCCTTCGGGGCCGGCGTGACGGCGATCGTCG
GGCGGGACGGCCGCGCCGCCGGCGTCGCCCTGGCGGACGGACGGGAACTCGCGGCCGACCTGATCCTCGTGGGGATCGGC
GTCCTGCCGAACCGGGAACTCGCCGCGGAGGCCGGCCTGCCGGCCGAGGACGGCGTGCGGGTCGACGCGTTCCTGGCGAC
GCCCGACCCGGCCATCTCGGCGATCGGCGACTGCGCCCGCTTCCCCATCCCCTTCGCCCGCGGGCTGACGCCGGACGGGA
CGGTGCGGATCGAGTCCGTGCAGAACGCCATCGACCAGGGCCGCTGCCTCGCGGCGCGGCTCACCGGCAAGCCCGCCTCC
TACGGCGCGCTGCCGTGGTTCTGGAGCGACCAGGGTCCCCACAAGCTGCAGATCGCCGGCCTGTCGGGGCCCGGCGATGC
CAGCGTCGTCCGCGGGACCGGCGACGCCTTCTCGGTCTTCCGCTTCCGCGACGGGCACCTGAGCGCGGTGGAATCCGTCG
CCCGGGCGGGCGACCACATGATCGCGCGGCGCCTGCTCACGGCCGGAACGCCGCTGACGCCCGACCAGGCGGCCGATCCG
GACTTCGACCTGAAAGGTCTGGCGCTGCGGCGGCCCTGA

Upstream 100 bases:

>100_bases
GTCCGGCCGCCTCCGCGGTCTGGTCCTACCTGATGTCGGAGGGCGCGCGCAGCCTCCCGGGCGTCGCCCAGAGCGGTCTC
GTGCCGGCGGGAGCCAGGGC

Downstream 100 bases:

>100_bases
CCTCAGCTGGGGAACGCCACCGGCGCCGGCTTCTGCCGCAGGGCGATGGCCGTGGCGATCCCGAGCGCCAGGATGGTGAC
GGCGCACCCCAGGAGCGCCC

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSAGVVVAGAGQAGFQLAASLREGGFRDPITLVGDEAALPYQRPPLSKAYLAGKTDQEGLFLRPPGFFAEHAVAHRPGLR
AVAIDRDARRLQLSDGTDLPYDHLVLATGARNRPLPVPGAELAGVRQLRGLADADALKESLATARAIVVVGAGFIGLEFA
AVAAARGLPVTVIEAAERPMARAVSSEMGAFFRRAHAAMGVRFAFGAGVTAIVGRDGRAAGVALADGRELAADLILVGIG
VLPNRELAAEAGLPAEDGVRVDAFLATPDPAISAIGDCARFPIPFARGLTPDGTVRIESVQNAIDQGRCLAARLTGKPAS
YGALPWFWSDQGPHKLQIAGLSGPGDASVVRGTGDAFSVFRFRDGHLSAVESVARAGDHMIARRLLTAGTPLTPDQAADP
DFDLKGLALRRP

Sequences:

>Translated_412_residues
MSAGVVVAGAGQAGFQLAASLREGGFRDPITLVGDEAALPYQRPPLSKAYLAGKTDQEGLFLRPPGFFAEHAVAHRPGLR
AVAIDRDARRLQLSDGTDLPYDHLVLATGARNRPLPVPGAELAGVRQLRGLADADALKESLATARAIVVVGAGFIGLEFA
AVAAARGLPVTVIEAAERPMARAVSSEMGAFFRRAHAAMGVRFAFGAGVTAIVGRDGRAAGVALADGRELAADLILVGIG
VLPNRELAAEAGLPAEDGVRVDAFLATPDPAISAIGDCARFPIPFARGLTPDGTVRIESVQNAIDQGRCLAARLTGKPAS
YGALPWFWSDQGPHKLQIAGLSGPGDASVVRGTGDAFSVFRFRDGHLSAVESVARAGDHMIARRLLTAGTPLTPDQAADP
DFDLKGLALRRP
>Mature_411_residues
SAGVVVAGAGQAGFQLAASLREGGFRDPITLVGDEAALPYQRPPLSKAYLAGKTDQEGLFLRPPGFFAEHAVAHRPGLRA
VAIDRDARRLQLSDGTDLPYDHLVLATGARNRPLPVPGAELAGVRQLRGLADADALKESLATARAIVVVGAGFIGLEFAA
VAAARGLPVTVIEAAERPMARAVSSEMGAFFRRAHAAMGVRFAFGAGVTAIVGRDGRAAGVALADGRELAADLILVGIGV
LPNRELAAEAGLPAEDGVRVDAFLATPDPAISAIGDCARFPIPFARGLTPDGTVRIESVQNAIDQGRCLAARLTGKPASY
GALPWFWSDQGPHKLQIAGLSGPGDASVVRGTGDAFSVFRFRDGHLSAVESVARAGDHMIARRLLTAGTPLTPDQAADPD
FDLKGLALRRP

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=353, Percent_Identity=30.8781869688385, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI65787454, Length=363, Percent_Identity=30.3030303030303, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI226437568, Length=363, Percent_Identity=30.3030303030303, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI22202629, Length=344, Percent_Identity=27.6162790697674, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4757732, Length=344, Percent_Identity=27.6162790697674, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1788892, Length=405, Percent_Identity=35.0617283950617, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789065, Length=289, Percent_Identity=31.8339100346021, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789765, Length=256, Percent_Identity=32.03125, Blast_Score=98, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=328, Percent_Identity=30.7926829268293, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24639257, Length=401, Percent_Identity=29.426433915212, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281359715, Length=401, Percent_Identity=29.426433915212, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI281359713, Length=401, Percent_Identity=29.426433915212, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24639250, Length=401, Percent_Identity=29.426433915212, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI18543267, Length=401, Percent_Identity=29.426433915212, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24639252, Length=401, Percent_Identity=29.426433915212, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24585130, Length=403, Percent_Identity=26.5508684863524, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24581020, Length=366, Percent_Identity=26.5027322404372, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI28573993, Length=366, Percent_Identity=26.2295081967213, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21358499, Length=247, Percent_Identity=29.1497975708502, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 42741; Mature: 42610

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGVVVAGAGQAGFQLAASLREGGFRDPITLVGDEAALPYQRPPLSKAYLAGKTDQEGL
CCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHCCCCCCCCCE
FLRPPGFFAEHAVAHRPGLRAVAIDRDARRLQLSDGTDLPYDHLVLATGARNRPLPVPGA
EECCCCCHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCH
ELAGVRQLRGLADADALKESLATARAIVVVGAGFIGLEFAAVAAARGLPVTVIEAAERPM
HHHHHHHHHCCCHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHCCCCEEEEHHHCCHH
ARAVSSEMGAFFRRAHAAMGVRFAFGAGVTAIVGRDGRAAGVALADGRELAADLILVGIG
HHHHHHHHHHHHHHHHHHHCEEEECCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHCC
VLPNRELAAEAGLPAEDGVRVDAFLATPDPAISAIGDCARFPIPFARGLTPDGTVRIESV
CCCCCHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCHHCCCCCCCCEEEHHH
QNAIDQGRCLAARLTGKPASYGALPWFWSDQGPHKLQIAGLSGPGDASVVRGTGDAFSVF
HHHHHCCCEEEEEECCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCEEEECCCCEEEEE
RFRDGHLSAVESVARAGDHMIARRLLTAGTPLTPDQAADPDFDLKGLALRRP
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure 
SAGVVVAGAGQAGFQLAASLREGGFRDPITLVGDEAALPYQRPPLSKAYLAGKTDQEGL
CCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHCCCCCCCCCE
FLRPPGFFAEHAVAHRPGLRAVAIDRDARRLQLSDGTDLPYDHLVLATGARNRPLPVPGA
EECCCCCHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCH
ELAGVRQLRGLADADALKESLATARAIVVVGAGFIGLEFAAVAAARGLPVTVIEAAERPM
HHHHHHHHHCCCHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHCCCCEEEEHHHCCHH
ARAVSSEMGAFFRRAHAAMGVRFAFGAGVTAIVGRDGRAAGVALADGRELAADLILVGIG
HHHHHHHHHHHHHHHHHHHCEEEECCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHCC
VLPNRELAAEAGLPAEDGVRVDAFLATPDPAISAIGDCARFPIPFARGLTPDGTVRIESV
CCCCCHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCHHCCCCCCCCEEEHHH
QNAIDQGRCLAARLTGKPASYGALPWFWSDQGPHKLQIAGLSGPGDASVVRGTGDAFSVF
HHHHHCCCEEEEEECCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCEEEECCCCEEEEE
RFRDGHLSAVESVARAGDHMIARRLLTAGTPLTPDQAADPDFDLKGLALRRP
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]