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The map label for this gene is ada [H]

Identifier: 170749951

GI number: 170749951

Start: 3770139

End: 3771248

Strand: Reverse

Name: ada [H]

Synonym: Mrad2831_3551

Alternate gene names: 170749951

Gene position: 3771248-3770139 (Counterclockwise)

Preceding gene: 170749953

Following gene: 170749948

Centisome position: 62.05

GC content: 77.03

Gene sequence:

>1110_bases
ATGCCCCTCCTCAACGCCTACGCCAAGCCGCATAGTGCCGCCGCCATGAGCCCTGTACCCGCGACAGACCCCGACGATGC
CGCGCGCTGGGCAGCCCTCGCCGCCCGCGACGCGCGGGCCGACGGCACCTTCGTGTACGCGGTCCGCACCACCGGGGTGT
ACTGCCGGCCGAGTTGCGCCGCGCGCGCGGCGCGCCCCGAGAATGTCAGCTTCCACCGGACCTGCGCCGAGGCCGAGGCG
GCCGGCTTCCGGCCGTGCCGGCGCTGTCGTCCGGACGAGCCCGCCCTCGCCGAGCGCCGGGCCGAGGCGGTCGCCCGCGC
CTGCCGCTCGATCGAGACAGCCGAGACGATGCCGTCCCTCGCCGCGCTCGCGCGCGCCGCCGGCCTGAGCGCCTACCACT
TCCACCGGGTTTTCAAGGACGTCACCGGCGTGACCCCCAAGGCCTACGCGGCCGCCCACCGCGCCGCCGCGGTGGCGCGG
CGGCTGCCCGGCGCCGCGTCGGTGACGGAGGCGCTCTACGAGGCCGGCTACGGCGCGGCGAGCCGGTTCTACGCGGGTGG
CGCGCCGCGCCTCGGCATGGCGCCCGCCGCCTACCGGACGGGCGGCGCGGGCCTGCGCATCCGGTTCGGCATCGGCGCCT
GCAGCCTCGGGGCGATCCTCGTCGCCGCCACCGAGGCGGGCGTCTGCGCGATCCTCCTCGGGGACGCGCCCGAGCCGCTC
CTGCACGATCTCCAGGACCGCTTCCCCGCCGCGGAGATCGAGGGCGGCGACCCGGCCTTCGAGGGCTGGATGGCGCAGGT
CATCGGGTTCGTGGAGGCCCCGGGCACCGGCCTCGACCTGCCGCTCGACATTCGCGGCACGGCGTTCCAGCAGCGGGTCT
GGGCGGCGCTGCGCGAGATCCCGGTCGGCTCCACCGCGACCTACGCGGAGATCGCCCGGGCGATCGGCCTTCCGACGGCG
ACGCGCGCCGTCGCGCAGGCCTGCGGGGCGAACCCGGTCGCGGTGGCCATTCCGTGCCACCGCGTCGTGCGCTCGGACGG
CGCCCTGTCGGGCTATCGCTGGGGCGTCGCGCGCAAGCGCGCGCTGCTCGATCGGGAGGCGGAGGCCTGA

Upstream 100 bases:

>100_bases
CGCCGCGGATATCGAATTCGGCCGCAAGGAGCGGGATGCCGGCGCGGCAGCGGACCGACACAGTCCTGCGCGCGTCATCC
ACCACCCGGCAGGCCCCGTC

Downstream 100 bases:

>100_bases
AGCGTCCCTGTCCCGCGCGCTCCGGTGCTCAGCACACGCTGCCCTAGCGTCGACCACCGCCGGGACCGCCAGGACCGCCG
AAGCCGCCGCCGAAGCCGCC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MPLLNAYAKPHSAAAMSPVPATDPDDAARWAALAARDARADGTFVYAVRTTGVYCRPSCAARAARPENVSFHRTCAEAEA
AGFRPCRRCRPDEPALAERRAEAVARACRSIETAETMPSLAALARAAGLSAYHFHRVFKDVTGVTPKAYAAAHRAAAVAR
RLPGAASVTEALYEAGYGAASRFYAGGAPRLGMAPAAYRTGGAGLRIRFGIGACSLGAILVAATEAGVCAILLGDAPEPL
LHDLQDRFPAAEIEGGDPAFEGWMAQVIGFVEAPGTGLDLPLDIRGTAFQQRVWAALREIPVGSTATYAEIARAIGLPTA
TRAVAQACGANPVAVAIPCHRVVRSDGALSGYRWGVARKRALLDREAEA

Sequences:

>Translated_369_residues
MPLLNAYAKPHSAAAMSPVPATDPDDAARWAALAARDARADGTFVYAVRTTGVYCRPSCAARAARPENVSFHRTCAEAEA
AGFRPCRRCRPDEPALAERRAEAVARACRSIETAETMPSLAALARAAGLSAYHFHRVFKDVTGVTPKAYAAAHRAAAVAR
RLPGAASVTEALYEAGYGAASRFYAGGAPRLGMAPAAYRTGGAGLRIRFGIGACSLGAILVAATEAGVCAILLGDAPEPL
LHDLQDRFPAAEIEGGDPAFEGWMAQVIGFVEAPGTGLDLPLDIRGTAFQQRVWAALREIPVGSTATYAEIARAIGLPTA
TRAVAQACGANPVAVAIPCHRVVRSDGALSGYRWGVARKRALLDREAEA
>Mature_368_residues
PLLNAYAKPHSAAAMSPVPATDPDDAARWAALAARDARADGTFVYAVRTTGVYCRPSCAARAARPENVSFHRTCAEAEAA
GFRPCRRCRPDEPALAERRAEAVARACRSIETAETMPSLAALARAAGLSAYHFHRVFKDVTGVTPKAYAAAHRAAAVARR
LPGAASVTEALYEAGYGAASRFYAGGAPRLGMAPAAYRTGGAGLRIRFGIGACSLGAILVAATEAGVCAILLGDAPEPLL
HDLQDRFPAAEIEGGDPAFEGWMAQVIGFVEAPGTGLDLPLDIRGTAFQQRVWAALREIPVGSTATYAEIARAIGLPTAT
RAVAQACGANPVAVAIPCHRVVRSDGALSGYRWGVARKRALLDREAEA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=126, Percent_Identity=34.9206349206349, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788542, Length=348, Percent_Identity=50.8620689655172, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1787596, Length=88, Percent_Identity=45.4545454545455, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=131, Percent_Identity=43.5114503816794, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=131, Percent_Identity=43.5114503816794, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=46.9879518072289, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137554, Length=172, Percent_Identity=34.8837209302326, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 38533; Mature: 38402

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLNAYAKPHSAAAMSPVPATDPDDAARWAALAARDARADGTFVYAVRTTGVYCRPSCA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCHHH
ARAARPENVSFHRTCAEAEAAGFRPCRRCRPDEPALAERRAEAVARACRSIETAETMPSL
HHCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALARAAGLSAYHFHRVFKDVTGVTPKAYAAAHRAAAVARRLPGAASVTEALYEAGYGAA
HHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHH
SRFYAGGAPRLGMAPAAYRTGGAGLRIRFGIGACSLGAILVAATEAGVCAILLGDAPEPL
HHHCCCCCCCCCCCCHHHCCCCCCEEEEEECCHHHHHHHHEEECCCCEEEEEECCCCCHH
LHDLQDRFPAAEIEGGDPAFEGWMAQVIGFVEAPGTGLDLPLDIRGTAFQQRVWAALREI
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
PVGSTATYAEIARAIGLPTATRAVAQACGANPVAVAIPCHRVVRSDGALSGYRWGVARKR
CCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCHHHHHHHHH
ALLDREAEA
HHHHHCCCC
>Mature Secondary Structure 
PLLNAYAKPHSAAAMSPVPATDPDDAARWAALAARDARADGTFVYAVRTTGVYCRPSCA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCHHH
ARAARPENVSFHRTCAEAEAAGFRPCRRCRPDEPALAERRAEAVARACRSIETAETMPSL
HHCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALARAAGLSAYHFHRVFKDVTGVTPKAYAAAHRAAAVARRLPGAASVTEALYEAGYGAA
HHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHH
SRFYAGGAPRLGMAPAAYRTGGAGLRIRFGIGACSLGAILVAATEAGVCAILLGDAPEPL
HHHCCCCCCCCCCCCHHHCCCCCCEEEEEECCHHHHHHHHEEECCCCEEEEEECCCCCHH
LHDLQDRFPAAEIEGGDPAFEGWMAQVIGFVEAPGTGLDLPLDIRGTAFQQRVWAALREI
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
PVGSTATYAEIARAIGLPTATRAVAQACGANPVAVAIPCHRVVRSDGALSGYRWGVARKR
CCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCHHHHHHHHH
ALLDREAEA
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]