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The map label for this gene is trpA

Identifier: 170749783

GI number: 170749783

Start: 3591360

End: 3592199

Strand: Reverse

Name: trpA

Synonym: Mrad2831_3382

Alternate gene names: 170749783

Gene position: 3592199-3591360 (Counterclockwise)

Preceding gene: 170749787

Following gene: 170749782

Centisome position: 59.1

GC content: 73.45

Gene sequence:

>840_bases
ATGCCGAGCCGCATCGACGCCACCTTCGCCCGCGCCCGCGCGGAGAACCGGTCCGTCCTCGTCACCTACGTGATGTCCGG
CGATCCCGATCCCGAAACCTCTCTGGAGGTCCTGAAGGCGCTGCCGGGCGCCGGCGCCGACATCCTGGAATTCGGCCTGC
CGTTCACCGACCCGATGGCGGACGGCCCGGCGATCCAGGCCGCCGGCCTGCGGGCGCTCAAGGCGGGCCAGACCGTGTCG
GGCACCCTCGACCTCGTGCGCCGCTTCCGCGCCGGCAACGACACCACGCCCGTCGTGCTGATGGGCTACTACAACCCGAT
CCACACCTACGGCGTCGACCGCTTCCTCGACGACGCGGTGGCGGCCGGCGTCGACGGGCTGATCGTCGTCGATCTCCCGC
CCGAGGAGGATGCCGAGCTGTGCCTGCCGGCCCTCGGCAAGGGCCTCGCCTTCATCCGCCTCGCCACGCCGACGACCGAC
GAGCGGCGTCTGCCGGCCGTCCTCGCGAACACGGCGGGCTTCGTCTACTACGTGTCGATCACCGGCATCACCGGGACGGC
GACACCGGATTTCGGCAAGGTCTCCGAGGCGGTCGCCCGGATCCGCCGCCACACAGACCTGCCCGTCGTCGTGGGCTTCG
GCGTCAAGACCGGCGCGCACGCCGCGGCGATCGCCAAGGGCGCCGACGGCGTCGTGGTCGGCTCCGCGCTGGTAGACGCC
CTGGTGCGCTCCCTCGACGGCGAGGGCCGGCCGCAGGCCGGAAGCGTCGGGGCGGTGACCGAGCTGGTGCGCGAACTCGC
CGCGGGCGTGCGGTCGGCGGGCGTCGGGCGGGCCGCCTGA

Upstream 100 bases:

>100_bases
TCGCCGCGGGCCCGCCCGCTTCACGGGCCGATGCCTTTGGTGTAGCGCGGGTGCAACCCCGGGCCGTCGGCGGCCCTGCA
GAAGCGAATATCGACCGACC

Downstream 100 bases:

>100_bases
CGGGCGGGCCGGGCACCCCTGGCGGCGGCGCGCGCCGCACCTGATATCTGCGCGGGCTTGAAGCGGACGGACGCGAGTCG
ATGGTCGAACCGATGAACTG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MPSRIDATFARARAENRSVLVTYVMSGDPDPETSLEVLKALPGAGADILEFGLPFTDPMADGPAIQAAGLRALKAGQTVS
GTLDLVRRFRAGNDTTPVVLMGYYNPIHTYGVDRFLDDAVAAGVDGLIVVDLPPEEDAELCLPALGKGLAFIRLATPTTD
ERRLPAVLANTAGFVYYVSITGITGTATPDFGKVSEAVARIRRHTDLPVVVGFGVKTGAHAAAIAKGADGVVVGSALVDA
LVRSLDGEGRPQAGSVGAVTELVRELAAGVRSAGVGRAA

Sequences:

>Translated_279_residues
MPSRIDATFARARAENRSVLVTYVMSGDPDPETSLEVLKALPGAGADILEFGLPFTDPMADGPAIQAAGLRALKAGQTVS
GTLDLVRRFRAGNDTTPVVLMGYYNPIHTYGVDRFLDDAVAAGVDGLIVVDLPPEEDAELCLPALGKGLAFIRLATPTTD
ERRLPAVLANTAGFVYYVSITGITGTATPDFGKVSEAVARIRRHTDLPVVVGFGVKTGAHAAAIAKGADGVVVGSALVDA
LVRSLDGEGRPQAGSVGAVTELVRELAAGVRSAGVGRAA
>Mature_278_residues
PSRIDATFARARAENRSVLVTYVMSGDPDPETSLEVLKALPGAGADILEFGLPFTDPMADGPAIQAAGLRALKAGQTVSG
TLDLVRRFRAGNDTTPVVLMGYYNPIHTYGVDRFLDDAVAAGVDGLIVVDLPPEEDAELCLPALGKGLAFIRLATPTTDE
RRLPAVLANTAGFVYYVSITGITGTATPDFGKVSEAVARIRRHTDLPVVVGFGVKTGAHAAAIAKGADGVVVGSALVDAL
VRSLDGEGRPQAGSVGAVTELVRELAAGVRSAGVGRAA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=213, Percent_Identity=34.2723004694836, Blast_Score=132, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=42.6778242677824, Blast_Score=198, Evalue=9e-52,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_METRJ (B1LSQ8)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001756043.1
- ProteinModelPortal:   B1LSQ8
- SMR:   B1LSQ8
- GeneID:   6139430
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_3382
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28747; Mature: 28616

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 50-50 ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRIDATFARARAENRSVLVTYVMSGDPDPETSLEVLKALPGAGADILEFGLPFTDPMA
CCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCC
DGPAIQAAGLRALKAGQTVSGTLDLVRRFRAGNDTTPVVLMGYYNPIHTYGVDRFLDDAV
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
AAGVDGLIVVDLPPEEDAELCLPALGKGLAFIRLATPTTDERRLPAVLANTAGFVYYVSI
HCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHCCCCEEEEEEE
TGITGTATPDFGKVSEAVARIRRHTDLPVVVGFGVKTGAHAAAIAKGADGVVVGSALVDA
ECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCCCEEEHHHHHHH
LVRSLDGEGRPQAGSVGAVTELVRELAAGVRSAGVGRAA
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PSRIDATFARARAENRSVLVTYVMSGDPDPETSLEVLKALPGAGADILEFGLPFTDPMA
CCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCC
DGPAIQAAGLRALKAGQTVSGTLDLVRRFRAGNDTTPVVLMGYYNPIHTYGVDRFLDDAV
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
AAGVDGLIVVDLPPEEDAELCLPALGKGLAFIRLATPTTDERRLPAVLANTAGFVYYVSI
HCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHCCCCEEEEEEE
TGITGTATPDFGKVSEAVARIRRHTDLPVVVGFGVKTGAHAAAIAKGADGVVVGSALVDA
ECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCCCEEEHHHHHHH
LVRSLDGEGRPQAGSVGAVTELVRELAAGVRSAGVGRAA
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA