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The map label for this gene is ttuE [H]

Identifier: 170749759

GI number: 170749759

Start: 3565248

End: 3566660

Strand: Direct

Name: ttuE [H]

Synonym: Mrad2831_3358

Alternate gene names: 170749759

Gene position: 3565248-3566660 (Clockwise)

Preceding gene: 170749758

Following gene: 170749760

Centisome position: 58.66

GC content: 71.41

Gene sequence:

>1413_bases
ATGCGCCGTCACCGCCACGCCAAGATCGTCGCCACCGTCGGTCCCGCCAGCTCCGCCCCGGACCAGCTCCGCGCCCTGTT
CCTCGCCGGCGTCGACACGTTCCGGCTCAATTTCAGCCACGGCCTCCAGGCGGATCACGCCAAGGTCCACGGCGCCATCC
GCGCCCTCGAGAAGGAGGTCGGCCGGCCGATCGGCATCCTGCAGGACCTGCAGGGGCCGAAGATCCGGATCGGCACGCTG
CAGGGCGGCCGGCTCGATCTCGAGGCCGGCGAGACGGTCCGCTTCGTCCTCGAGGGCGCCGACGGCGACAAGCAGGCGAT
CCCGCTGCACCATCCGGAGATCTTCGACGCCGTCGTGCCGGGCCAGGAACTCCTCATCGACGACGGCCGGGTGCGGGTGC
GGGTCACCGGGCCCGAGCGGACCTCGATCACCGCCGAGGTCGTCACCGGCGGGCCGATCTCGAACCGCAAGGGCGTGAAC
CTGCCGGGTACGCTGCTGGACCTGTCGCCGCTCACCGAGAAGGACCGGGCGGACCTCGCCTTCGGGCTCGATCTCGGCGT
CGACTGGGTCGCCCTGTCCTTCGTCCAGAAGCCGTCCGACGTCATCGAGGCGCGGGGAATCATCGGCGATCGGGCCGGCA
TCATGTCGAAGATCGAGAAGCCGCAGGCGCTGGAGCGGATCGCCGACATCATCCGGCTCTCCGACGCCGTGATGGTCGCC
CGCGGCGACCTCGGCGTCGAGATCCCGCACGAGGACGTGCCGGGGCGGCAGAAGGAGCTGATCCGGGCCTGCCGGCTCGC
CGTGAAGCCCGTCGTGGTGGCGACCCAGATGCTCGACTCGATGGTCAACGCCCCCGCGCCCACCCGGGCGGAGGCGTCGG
ACGTCGCCACCGCCATCTATGACGGCGCCGACGCCGTGATGCTGTCGGCGGAATCCGCCACAGGCCGCTACCCGGTCGAG
GCGGTGGCGATGATGGATCGGATCATCCGGAGCGTGGAGGGGCACAAGCTCTACCACTCGATCGTCGCGGCCTCGGAGCC
GGGCGAGGAGGAGACGCCGCCCCACGCGGTGGCGACCGCCACGGCCGACCTCGCCGAGGCCGTGCACGCCGCCGCGATCG
TCGCCTACACGGCGAGCGGGACGACGGCCGCGCGGGTCGCGCGCAAGCGCCCCGCCGCGTCGATCCTGGCCCTGACCCCC
AACGTCGCGACCTCGCGGCGGCTGAGCCTGCTGTGGGGGGCGCACAGCGTCCTGACGGAGGACGTCGACAGCTACGAGGA
GATGACCTCGAAGGCCTGCCACCACGCGCAGGACGAGGGCTTCGCGCGGTCGAACGACATCATCGTGGTCACCGCCGGCA
TCCCGTTCCACACCGCCGGCAACACCAACAACATCCGCCTCATGCAGGTCTGA

Upstream 100 bases:

>100_bases
GGTCTCGCCGCGGGGCCGTCGCGGACCGATGTGAGCGCCGTCGGCGCGAGCGAGCGCCCGGCTCGATTCCCAGAGGCAGC
CTCAGGAGGCCGAGACCCGC

Downstream 100 bases:

>100_bases
GGCGCCCGATCGGGCGCCGGGCTGGAACGCGGCCCGCGCCTGACCCGTTGTTGGGGAAGCTGCGCCATTTGGGCGCGTGA
CCGGAGGGCCGACCCATGAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MRRHRHAKIVATVGPASSAPDQLRALFLAGVDTFRLNFSHGLQADHAKVHGAIRALEKEVGRPIGILQDLQGPKIRIGTL
QGGRLDLEAGETVRFVLEGADGDKQAIPLHHPEIFDAVVPGQELLIDDGRVRVRVTGPERTSITAEVVTGGPISNRKGVN
LPGTLLDLSPLTEKDRADLAFGLDLGVDWVALSFVQKPSDVIEARGIIGDRAGIMSKIEKPQALERIADIIRLSDAVMVA
RGDLGVEIPHEDVPGRQKELIRACRLAVKPVVVATQMLDSMVNAPAPTRAEASDVATAIYDGADAVMLSAESATGRYPVE
AVAMMDRIIRSVEGHKLYHSIVAASEPGEEETPPHAVATATADLAEAVHAAAIVAYTASGTTAARVARKRPAASILALTP
NVATSRRLSLLWGAHSVLTEDVDSYEEMTSKACHHAQDEGFARSNDIIVVTAGIPFHTAGNTNNIRLMQV

Sequences:

>Translated_470_residues
MRRHRHAKIVATVGPASSAPDQLRALFLAGVDTFRLNFSHGLQADHAKVHGAIRALEKEVGRPIGILQDLQGPKIRIGTL
QGGRLDLEAGETVRFVLEGADGDKQAIPLHHPEIFDAVVPGQELLIDDGRVRVRVTGPERTSITAEVVTGGPISNRKGVN
LPGTLLDLSPLTEKDRADLAFGLDLGVDWVALSFVQKPSDVIEARGIIGDRAGIMSKIEKPQALERIADIIRLSDAVMVA
RGDLGVEIPHEDVPGRQKELIRACRLAVKPVVVATQMLDSMVNAPAPTRAEASDVATAIYDGADAVMLSAESATGRYPVE
AVAMMDRIIRSVEGHKLYHSIVAASEPGEEETPPHAVATATADLAEAVHAAAIVAYTASGTTAARVARKRPAASILALTP
NVATSRRLSLLWGAHSVLTEDVDSYEEMTSKACHHAQDEGFARSNDIIVVTAGIPFHTAGNTNNIRLMQV
>Mature_470_residues
MRRHRHAKIVATVGPASSAPDQLRALFLAGVDTFRLNFSHGLQADHAKVHGAIRALEKEVGRPIGILQDLQGPKIRIGTL
QGGRLDLEAGETVRFVLEGADGDKQAIPLHHPEIFDAVVPGQELLIDDGRVRVRVTGPERTSITAEVVTGGPISNRKGVN
LPGTLLDLSPLTEKDRADLAFGLDLGVDWVALSFVQKPSDVIEARGIIGDRAGIMSKIEKPQALERIADIIRLSDAVMVA
RGDLGVEIPHEDVPGRQKELIRACRLAVKPVVVATQMLDSMVNAPAPTRAEASDVATAIYDGADAVMLSAESATGRYPVE
AVAMMDRIIRSVEGHKLYHSIVAASEPGEEETPPHAVATATADLAEAVHAAAIVAYTASGTTAARVARKRPAASILALTP
NVATSRRLSLLWGAHSVLTEDVDSYEEMTSKACHHAQDEGFARSNDIIVVTAGIPFHTAGNTNNIRLMQV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=37.2708757637475, Blast_Score=300, Evalue=3e-81,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=37.2708757637475, Blast_Score=300, Evalue=3e-81,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=36.7676767676768, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=38.3367139959432, Blast_Score=292, Evalue=5e-79,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=38.3367139959432, Blast_Score=292, Evalue=5e-79,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=37.2822299651568, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=37.2822299651568, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=33.7552742616034, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=33.7552742616034, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=33.1775700934579, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1787965, Length=467, Percent_Identity=38.9721627408994, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=38.3817427385892, Blast_Score=290, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=486, Percent_Identity=36.4197530864198, Blast_Score=277, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=36.9834710743802, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=36.9834710743802, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=36.9609856262834, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=36.9834710743802, Blast_Score=262, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6324923, Length=484, Percent_Identity=35.1239669421488, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=34.0862422997947, Blast_Score=239, Evalue=9e-64,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=36.0323886639676, Blast_Score=281, Evalue=9e-76,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=36.0323886639676, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24648966, Length=409, Percent_Identity=40.5867970660147, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24581235, Length=488, Percent_Identity=32.5819672131148, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI24646914, Length=285, Percent_Identity=40.3508771929825, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50100; Mature: 50100

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRHRHAKIVATVGPASSAPDQLRALFLAGVDTFRLNFSHGLQADHAKVHGAIRALEKEV
CCCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
GRPIGILQDLQGPKIRIGTLQGGRLDLEAGETVRFVLEGADGDKQAIPLHHPEIFDAVVP
CCCCCHHHCCCCCEEEEEECCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCC
GQELLIDDGRVRVRVTGPERTSITAEVVTGGPISNRKGVNLPGTLLDLSPLTEKDRADLA
CCEEEEECCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCEE
FGLDLGVDWVALSFVQKPSDVIEARGIIGDRAGIMSKIEKPQALERIADIIRLSDAVMVA
EECCCCHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCEEEEE
RGDLGVEIPHEDVPGRQKELIRACRLAVKPVVVATQMLDSMVNAPAPTRAEASDVATAIY
ECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DGADAVMLSAESATGRYPVEAVAMMDRIIRSVEGHKLYHSIVAASEPGEEETPPHAVATA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHH
TADLAEAVHAAAIVAYTASGTTAARVARKRPAASILALTPNVATSRRLSLLWGAHSVLTE
HHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
DVDSYEEMTSKACHHAQDEGFARSNDIIVVTAGIPFHTAGNTNNIRLMQV
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECCCCCCEEEEEC
>Mature Secondary Structure
MRRHRHAKIVATVGPASSAPDQLRALFLAGVDTFRLNFSHGLQADHAKVHGAIRALEKEV
CCCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
GRPIGILQDLQGPKIRIGTLQGGRLDLEAGETVRFVLEGADGDKQAIPLHHPEIFDAVVP
CCCCCHHHCCCCCEEEEEECCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCC
GQELLIDDGRVRVRVTGPERTSITAEVVTGGPISNRKGVNLPGTLLDLSPLTEKDRADLA
CCEEEEECCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCEE
FGLDLGVDWVALSFVQKPSDVIEARGIIGDRAGIMSKIEKPQALERIADIIRLSDAVMVA
EECCCCHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCEEEEE
RGDLGVEIPHEDVPGRQKELIRACRLAVKPVVVATQMLDSMVNAPAPTRAEASDVATAIY
ECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DGADAVMLSAESATGRYPVEAVAMMDRIIRSVEGHKLYHSIVAASEPGEEETPPHAVATA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHH
TADLAEAVHAAAIVAYTASGTTAARVARKRPAASILALTPNVATSRRLSLLWGAHSVLTE
HHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
DVDSYEEMTSKACHHAQDEGFARSNDIIVVTAGIPFHTAGNTNNIRLMQV
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8672817 [H]