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The map label for this gene is exoT [H]

Identifier: 170749749

GI number: 170749749

Start: 3554924

End: 3556432

Strand: Direct

Name: exoT [H]

Synonym: Mrad2831_3348

Alternate gene names: 170749749

Gene position: 3554924-3556432 (Clockwise)

Preceding gene: 170749747

Following gene: 170749750

Centisome position: 58.49

GC content: 72.03

Gene sequence:

>1509_bases
ATGGCAGAACTCCGAGAGGGGCCGGAAGCGACGGCGCCGCCCGCGTTCTCGAAGCGCCGCAGCCTGGCCTGGATGGGATT
CTGCCAGGGCGGCCTGTTCCTGGCACAGTTCGGTACCTCGCTCGCCCTGGCGCGCCTGCTCACCCCCCACGAGACCGGCA
TCTTCAGCCTCGCGGCCGCCATCGCGGGCCTGCTGTCGACGCTGCGCTCCTGCGGCCTATCGAGCTACATCGTCCGCGCG
GAGCGGGTGGACGCGGCGCTGCTGGCGAGCGTCTTCACGGTGAACCTGATGCTGTCGGGCGCGGCGGCGATCCTGATCCT
GGCCTTCAGCGTGTTCGGCAGCGTTCTCCTCGGTGAGCCGGAGGTCCAGCGCGCCCTCGCCATCCTCGCCGTGGTGCCCC
TCATCGGCGCACTGGAGTTCCGCCCGGCCGCCATGATCGAGCGGCACGGCAATTTCCGCGGCGTGGCCCTGGTCAACATG
CTGCGCGGTCTCGTCGCCAGTGGCGCGATGCTGGCGCTGGCCCTCCTCGGCTTCAGCTACATGAGCCAGGCCTACGGGCA
GGCCGCGGGCGCCGTCGCGGCGGCGCTGGCTGCGAACGGCCTCGGGCTGCGCTACGTGTCGCTGCGCCTCGGGCTCGCGG
GCTGGCGCGAGATCCTGACCTACGGCGGCCGGCTCTTCGCCATCGCGGGGGTCGCGGGGATCGCCGGCCGGATGGGCGAC
CTCGTCCTCGGGCGGATGCTCGGGCTCAGCGCCCTCGGCCTCTACTCCCGCGCGGCGAGCCTCAACACCCTGATGTGGGA
CCACCTCCACGTGGTGATCACCCGCATCACCTTCGTGGACCTCGCGAATCAGCAGCGGAACGGACAGTCCCTGCGCGCGT
GCTACCTCCACACGCTGCGGATGATCACCGTGCTGCTCTGGCCGGCCTTCGCGGGCGCGGCCGTCCTGGCCGGGCCGATC
GTGCGCCTCCTGCTCGGTCCGGGCTGGGACGGCGCGGCGCTGCCCTTCTGCCTGCTGTCCGTGGCCGCGATCGTCCTGAG
TTCGCTCAGCATGACCTGGGAGGTGTTCCTGATCCGGGATCAGACCGCCCTCCAGGCGCGGTTCGAGTTCCTGCGCCACG
GCGCCGGCTTCGTGATGTTCAGCGTCGGCTGCCTGTTCGGCCTCGGCGGCGCGGGCGCGGCACGGATCGGCGAGGCGCTC
CTGGCCGTGGGACTCTATCGGCCGCATCTCGAGCGGATGACCGACACCTTCAGGCGCGACTACGCCCCCATCTACCTCCA
CGCCGCCACACTGACGGCGATCGCCGTCGCACCCGCCATCCTGGTCATGTCGGCTTGGGGATGGTCGGCCGAGACGCCGC
TGCCGAGCCTCGCGGCCGCGATCGCCGCCGGGGTCGCCGGCTGGGCCTGCGGCCTCTGGTACCTGGACCATCCCCTGGTC
GCGGAAGCGCGCCTGCTACTCGCGCACATGCGCCCGGCCCGCCCGGCCACGACCGTGTCGGACCTCTAG

Upstream 100 bases:

>100_bases
CCAGCTGCCACGGTCGACCGGGCATTGTTAGACCGTTCTGACCGATCGCGCCCTGGCCGCAGCCAGCGCCGCCTCCACCC
TGTCGCGTCCGAAGGATCCC

Downstream 100 bases:

>100_bases
CGGCGCGCCGCGGCGCGAGCGTCAGGTCGTCCACCCAGGACGAGAGCAGCCGCACGCTTGCGCTTCCGCGCGCCGGGGCG
CGATATGACCGGACACGACG

Product: polysaccharide biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MAELREGPEATAPPAFSKRRSLAWMGFCQGGLFLAQFGTSLALARLLTPHETGIFSLAAAIAGLLSTLRSCGLSSYIVRA
ERVDAALLASVFTVNLMLSGAAAILILAFSVFGSVLLGEPEVQRALAILAVVPLIGALEFRPAAMIERHGNFRGVALVNM
LRGLVASGAMLALALLGFSYMSQAYGQAAGAVAAALAANGLGLRYVSLRLGLAGWREILTYGGRLFAIAGVAGIAGRMGD
LVLGRMLGLSALGLYSRAASLNTLMWDHLHVVITRITFVDLANQQRNGQSLRACYLHTLRMITVLLWPAFAGAAVLAGPI
VRLLLGPGWDGAALPFCLLSVAAIVLSSLSMTWEVFLIRDQTALQARFEFLRHGAGFVMFSVGCLFGLGGAGAARIGEAL
LAVGLYRPHLERMTDTFRRDYAPIYLHAATLTAIAVAPAILVMSAWGWSAETPLPSLAAAIAAGVAGWACGLWYLDHPLV
AEARLLLAHMRPARPATTVSDL

Sequences:

>Translated_502_residues
MAELREGPEATAPPAFSKRRSLAWMGFCQGGLFLAQFGTSLALARLLTPHETGIFSLAAAIAGLLSTLRSCGLSSYIVRA
ERVDAALLASVFTVNLMLSGAAAILILAFSVFGSVLLGEPEVQRALAILAVVPLIGALEFRPAAMIERHGNFRGVALVNM
LRGLVASGAMLALALLGFSYMSQAYGQAAGAVAAALAANGLGLRYVSLRLGLAGWREILTYGGRLFAIAGVAGIAGRMGD
LVLGRMLGLSALGLYSRAASLNTLMWDHLHVVITRITFVDLANQQRNGQSLRACYLHTLRMITVLLWPAFAGAAVLAGPI
VRLLLGPGWDGAALPFCLLSVAAIVLSSLSMTWEVFLIRDQTALQARFEFLRHGAGFVMFSVGCLFGLGGAGAARIGEAL
LAVGLYRPHLERMTDTFRRDYAPIYLHAATLTAIAVAPAILVMSAWGWSAETPLPSLAAAIAAGVAGWACGLWYLDHPLV
AEARLLLAHMRPARPATTVSDL
>Mature_501_residues
AELREGPEATAPPAFSKRRSLAWMGFCQGGLFLAQFGTSLALARLLTPHETGIFSLAAAIAGLLSTLRSCGLSSYIVRAE
RVDAALLASVFTVNLMLSGAAAILILAFSVFGSVLLGEPEVQRALAILAVVPLIGALEFRPAAMIERHGNFRGVALVNML
RGLVASGAMLALALLGFSYMSQAYGQAAGAVAAALAANGLGLRYVSLRLGLAGWREILTYGGRLFAIAGVAGIAGRMGDL
VLGRMLGLSALGLYSRAASLNTLMWDHLHVVITRITFVDLANQQRNGQSLRACYLHTLRMITVLLWPAFAGAAVLAGPIV
RLLLGPGWDGAALPFCLLSVAAIVLSSLSMTWEVFLIRDQTALQARFEFLRHGAGFVMFSVGCLFGLGGAGAARIGEALL
AVGLYRPHLERMTDTFRRDYAPIYLHAATLTAIAVAPAILVMSAWGWSAETPLPSLAAAIAAGVAGWACGLWYLDHPLVA
EARLLLAHMRPARPATTVSDL

Specific function: Lipopolysaccharide biosynthesis. [C]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788359, Length=472, Percent_Identity=21.6101694915254, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 52932; Mature: 52801

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELREGPEATAPPAFSKRRSLAWMGFCQGGLFLAQFGTSLALARLLTPHETGIFSLAAA
CCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IAGLLSTLRSCGLSSYIVRAERVDAALLASVFTVNLMLSGAAAILILAFSVFGSVLLGEP
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EVQRALAILAVVPLIGALEFRPAAMIERHGNFRGVALVNMLRGLVASGAMLALALLGFSY
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSQAYGQAAGAVAAALAANGLGLRYVSLRLGLAGWREILTYGGRLFAIAGVAGIAGRMGD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LVLGRMLGLSALGLYSRAASLNTLMWDHLHVVITRITFVDLANQQRNGQSLRACYLHTLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
MITVLLWPAFAGAAVLAGPIVRLLLGPGWDGAALPFCLLSVAAIVLSSLSMTWEVFLIRD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEC
QTALQARFEFLRHGAGFVMFSVGCLFGLGGAGAARIGEALLAVGLYRPHLERMTDTFRRD
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YAPIYLHAATLTAIAVAPAILVMSAWGWSAETPLPSLAAAIAAGVAGWACGLWYLDHPLV
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
AEARLLLAHMRPARPATTVSDL
HHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AELREGPEATAPPAFSKRRSLAWMGFCQGGLFLAQFGTSLALARLLTPHETGIFSLAAA
CCCCCCCCCCCCCCHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH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HHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226645; 8226646; 8118055; 11481431 [H]