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The map label for this gene is dppA [H]

Identifier: 170749685

GI number: 170749685

Start: 3481654

End: 3483291

Strand: Reverse

Name: dppA [H]

Synonym: Mrad2831_3283

Alternate gene names: 170749685

Gene position: 3483291-3481654 (Counterclockwise)

Preceding gene: 170749686

Following gene: 170749682

Centisome position: 57.31

GC content: 74.36

Gene sequence:

>1638_bases
GTGAGGCCGGCCGCGGCATGCCGGGCCGGGCTCCTCGCCCTGGCGGCCGGCCTGGCTTCCGGCCCGCTTTTCGACCCCGG
CCCGGCGGCGGCGCGCACCTTCGTGTTCTGCTCGGAGGGCAGCCCCGAATCCTTCGATCCCGCCCGCGCCACGACCACCA
CCACGATGAACGCCGCGTGGCAGGTCTACAACACGCTGGTGGAGTTCGCGCCCGGCACGACGACGATCCGCCCGGCCCTC
GCCGAATCCTGGACGATCTCGGAGGACGGCCGGACCTACGTCTTCGCCCTGCGGGACGGCGTCCGCTTCCACGCCAACGC
GCGCTTCACCCCCACGCGGCCCCTGAACGCCGACGACGTGGTGTTCTCCTTCGCCCGGCAGCGGCGCTCCGGACCCGACG
GCGCCGCGCCGGGCTTCCCCTACTTCCACGACCTCGGCCTCGCCGACCTGATCGAGGCCGTCGACGCCCCGGATCCGCGC
CACGTCCGGTTCCGGCTGCGCGAGGCCGACACCACCTTCCTCGCCAACATCGCCCAGGCCTTTGGCGCGATCCTGTCGGC
CGAGTACGCGGCGACCCTCGCGGCCGGCAACGCGTCGGACGACATCGCGCGGGCGCCGATCGGGACCGGCCCGTTCGTGT
TCGAGGGGTACCGGCCGGATCAGGTCCTGCGCTACCGCGCCTTCCCGGAATACTGGCGCCGCGCCGCGGACGACGGCGAA
CCGGGCGAGCGCCCCGACGGCCTCGTCTTCGCGATCACGCCGAACGCGGCGGTCCGGCTGACCAAGCTGCGGGCCGGCGA
GTGCCACGCGATGGCGTTTCCGGCGACCGGCGATCTCGACGCGATCCGGGCGGAGCCGGGCCTGACGCTGCTCGCGCTCG
CCGAGCTGAACGTCGCCTACCTCGCCCTCAACACGACCCGCTCCCCCTTCGGAGACGTCCGGGTGCGGCGCGCGGTCAAC
CTCGCCATCGACCGGCGGGCGATCGTGGCGGGGGTCTACGGCGATGCCGGGATCCTGGCTCAGGGGCCGCTGCCGCCGGG
GATCTGGGCCTACGACGCCGACCTGCCGGACACCCCCTTCGACCGCGGCGAGGCGCTGCGCCTGCTGGCCGAGGCCGGGC
TCGCCGAGGGCTTCGACGTCGAGCTGTGGTATCCGCCGGTCAGCCGGCCCTACAATCCGAACGGCCGGCGCGTCGCCGAC
ATGATCCAGGCCGACCTCGCGCAGGTCGGCATCCGCGCGCGCCCGGTCACGCGCCCCTGGAACGCGTACCGCGCCGCCCT
GTACGGCGGAGAGCCCGCGCTGATGCTCTACGGCTGGACGAGCGACAACGGCGACCCGGACAACTTCCTGAGCGTCCTGC
TCGGCTGCAAGGCCGCCCAGACCGGCGGCGCCAACCTCGCCCGCTGGTGCGACCCGGCCTACGACCGGCTGGTCGAGGCC
GCCCGCCGCACCGGCGACCGGGCGGCGCGGCAGGCGCTGTACCGTCAGGCCCAGGCCATCGTCCGGCACGCGATGCCCTG
GGTGCCCCTGGCCCACACCAAGGTGCATCTGGCGCTCCGCAACGACGTGCGCGGCCTGACGATGGATCCCCTCGGCCGCC
ACCTCTTCGAGACGGTGCGGTTCCGCGCCGCCCGGTGA

Upstream 100 bases:

>100_bases
TCAGCAGCGAGCGCGGTCAGGGGACCGTCGTGCAGGTCGATTTTCCCGCAGAGGGCGCGGGAGCGGCCGCGCGGCCGCGG
ACCAGCGCGATCGGCACGCC

Downstream 100 bases:

>100_bases
CGACGGCGGCGCGGGCCGACCTATCCGCCACGCTCACTTCTTGGTCCGCTGCTGGAAGACCCGCAGCACTTGGTTGCCCC
GTGCCACGGCGTCGTCGAGG

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MRPAAACRAGLLALAAGLASGPLFDPGPAAARTFVFCSEGSPESFDPARATTTTTMNAAWQVYNTLVEFAPGTTTIRPAL
AESWTISEDGRTYVFALRDGVRFHANARFTPTRPLNADDVVFSFARQRRSGPDGAAPGFPYFHDLGLADLIEAVDAPDPR
HVRFRLREADTTFLANIAQAFGAILSAEYAATLAAGNASDDIARAPIGTGPFVFEGYRPDQVLRYRAFPEYWRRAADDGE
PGERPDGLVFAITPNAAVRLTKLRAGECHAMAFPATGDLDAIRAEPGLTLLALAELNVAYLALNTTRSPFGDVRVRRAVN
LAIDRRAIVAGVYGDAGILAQGPLPPGIWAYDADLPDTPFDRGEALRLLAEAGLAEGFDVELWYPPVSRPYNPNGRRVAD
MIQADLAQVGIRARPVTRPWNAYRAALYGGEPALMLYGWTSDNGDPDNFLSVLLGCKAAQTGGANLARWCDPAYDRLVEA
ARRTGDRAARQALYRQAQAIVRHAMPWVPLAHTKVHLALRNDVRGLTMDPLGRHLFETVRFRAAR

Sequences:

>Translated_545_residues
MRPAAACRAGLLALAAGLASGPLFDPGPAAARTFVFCSEGSPESFDPARATTTTTMNAAWQVYNTLVEFAPGTTTIRPAL
AESWTISEDGRTYVFALRDGVRFHANARFTPTRPLNADDVVFSFARQRRSGPDGAAPGFPYFHDLGLADLIEAVDAPDPR
HVRFRLREADTTFLANIAQAFGAILSAEYAATLAAGNASDDIARAPIGTGPFVFEGYRPDQVLRYRAFPEYWRRAADDGE
PGERPDGLVFAITPNAAVRLTKLRAGECHAMAFPATGDLDAIRAEPGLTLLALAELNVAYLALNTTRSPFGDVRVRRAVN
LAIDRRAIVAGVYGDAGILAQGPLPPGIWAYDADLPDTPFDRGEALRLLAEAGLAEGFDVELWYPPVSRPYNPNGRRVAD
MIQADLAQVGIRARPVTRPWNAYRAALYGGEPALMLYGWTSDNGDPDNFLSVLLGCKAAQTGGANLARWCDPAYDRLVEA
ARRTGDRAARQALYRQAQAIVRHAMPWVPLAHTKVHLALRNDVRGLTMDPLGRHLFETVRFRAAR
>Mature_545_residues
MRPAAACRAGLLALAAGLASGPLFDPGPAAARTFVFCSEGSPESFDPARATTTTTMNAAWQVYNTLVEFAPGTTTIRPAL
AESWTISEDGRTYVFALRDGVRFHANARFTPTRPLNADDVVFSFARQRRSGPDGAAPGFPYFHDLGLADLIEAVDAPDPR
HVRFRLREADTTFLANIAQAFGAILSAEYAATLAAGNASDDIARAPIGTGPFVFEGYRPDQVLRYRAFPEYWRRAADDGE
PGERPDGLVFAITPNAAVRLTKLRAGECHAMAFPATGDLDAIRAEPGLTLLALAELNVAYLALNTTRSPFGDVRVRRAVN
LAIDRRAIVAGVYGDAGILAQGPLPPGIWAYDADLPDTPFDRGEALRLLAEAGLAEGFDVELWYPPVSRPYNPNGRRVAD
MIQADLAQVGIRARPVTRPWNAYRAALYGGEPALMLYGWTSDNGDPDNFLSVLLGCKAAQTGGANLARWCDPAYDRLVEA
ARRTGDRAARQALYRQAQAIVRHAMPWVPLAHTKVHLALRNDVRGLTMDPLGRHLFETVRFRAAR

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=541, Percent_Identity=42.8835489833641, Blast_Score=463, Evalue=1e-131,
Organism=Escherichia coli, GI1787551, Length=538, Percent_Identity=36.2453531598513, Blast_Score=309, Evalue=4e-85,
Organism=Escherichia coli, GI1787052, Length=541, Percent_Identity=28.0961182994455, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1787762, Length=543, Percent_Identity=25.7826887661142, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1789397, Length=547, Percent_Identity=24.1316270566728, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1789887, Length=526, Percent_Identity=25.0950570342205, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787495, Length=537, Percent_Identity=22.9050279329609, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081878, Length=527, Percent_Identity=24.2884250474383, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59126; Mature: 59126

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAAACRAGLLALAAGLASGPLFDPGPAAARTFVFCSEGSPESFDPARATTTTTMNAAW
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCEEEEHHHHHH
QVYNTLVEFAPGTTTIRPALAESWTISEDGRTYVFALRDGVRFHANARFTPTRPLNADDV
HHHHHHHHHCCCCCEECHHHHCCEEECCCCCEEEEEEECCEEEECCCEECCCCCCCHHHH
VFSFARQRRSGPDGAAPGFPYFHDLGLADLIEAVDAPDPRHVRFRLREADTTFLANIAQA
HHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHH
FGAILSAEYAATLAAGNASDDIARAPIGTGPFVFEGYRPDQVLRYRAFPEYWRRAADDGE
HHHHHHHHHHHHEECCCCCCCHHCCCCCCCCEEEECCCCHHHHHHHCCHHHHHHCCCCCC
PGERPDGLVFAITPNAAVRLTKLRAGECHAMAFPATGDLDAIRAEPGLTLLALAELNVAY
CCCCCCCEEEEECCCCEEEEEEECCCCEEEEECCCCCCCHHEECCCCCEEEEEECCCEEE
LALNTTRSPFGDVRVRRAVNLAIDRRAIVAGVYGDAGILAQGPLPPGIWAYDADLPDTPF
EEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEECCCCCCCCEEECCCCCCCCC
DRGEALRLLAEAGLAEGFDVELWYPPVSRPYNPNGRRVADMIQADLAQVGIRARPVTRPW
CCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
NAYRAALYGGEPALMLYGWTSDNGDPDNFLSVLLGCKAAQTGGANLARWCDPAYDRLVEA
HHHHHEEECCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHH
ARRTGDRAARQALYRQAQAIVRHAMPWVPLAHTKVHLALRNDVRGLTMDPLGRHLFETVR
HHHCCHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCCCHHHHHHHHHHH
FRAAR
HHCCC
>Mature Secondary Structure
MRPAAACRAGLLALAAGLASGPLFDPGPAAARTFVFCSEGSPESFDPARATTTTTMNAAW
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCEEEEHHHHHH
QVYNTLVEFAPGTTTIRPALAESWTISEDGRTYVFALRDGVRFHANARFTPTRPLNADDV
HHHHHHHHHCCCCCEECHHHHCCEEECCCCCEEEEEEECCEEEECCCEECCCCCCCHHHH
VFSFARQRRSGPDGAAPGFPYFHDLGLADLIEAVDAPDPRHVRFRLREADTTFLANIAQA
HHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHH
FGAILSAEYAATLAAGNASDDIARAPIGTGPFVFEGYRPDQVLRYRAFPEYWRRAADDGE
HHHHHHHHHHHHEECCCCCCCHHCCCCCCCCEEEECCCCHHHHHHHCCHHHHHHCCCCCC
PGERPDGLVFAITPNAAVRLTKLRAGECHAMAFPATGDLDAIRAEPGLTLLALAELNVAY
CCCCCCCEEEEECCCCEEEEEEECCCCEEEEECCCCCCCHHEECCCCCEEEEEECCCEEE
LALNTTRSPFGDVRVRRAVNLAIDRRAIVAGVYGDAGILAQGPLPPGIWAYDADLPDTPF
EEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEECCCCCCCCEEECCCCCCCCC
DRGEALRLLAEAGLAEGFDVELWYPPVSRPYNPNGRRVADMIQADLAQVGIRARPVTRPW
CCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
NAYRAALYGGEPALMLYGWTSDNGDPDNFLSVLLGCKAAQTGGANLARWCDPAYDRLVEA
HHHHHEEECCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHH
ARRTGDRAARQALYRQAQAIVRHAMPWVPLAHTKVHLALRNDVRGLTMDPLGRHLFETVR
HHHCCHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCCCHHHHHHHHHHH
FRAAR
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]