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The map label for this gene is Hgd [H]

Identifier: 170749657

GI number: 170749657

Start: 3447732

End: 3448604

Strand: Reverse

Name: Hgd [H]

Synonym: Mrad2831_3254

Alternate gene names: 170749657

Gene position: 3448604-3447732 (Counterclockwise)

Preceding gene: 170749658

Following gene: 170749656

Centisome position: 56.74

GC content: 71.59

Gene sequence:

>873_bases
ATGGAACAGAGAACCATCGGCGTCATCGGTCTCGGCATGATGGGCGACCCCATGAGCCGGTGCCTCGCCAAGGCGGGGCA
CGTGCTCCGCCTGCTCGACGCGGACGTAGGCCGGACCGACGCGCTCGCCCGGGACCTCGGCGCGACGGCGCTCACGCGGG
AGAACGCCGCCGGGCTCGACCTGCTGATCACGATGCTGCCGAACTCGGCCATCGTCGAGGCGGTGCTGCTCGATGATGGC
TGGGCCGACGCGCTTCCGGCCGGCGCCACCGTGATCGACATGAGCTCGTCGGAGCCGGTGCGGTCCCGCGCGCTCGGCGA
GACGCTCGCCGGGAAGGGGCTCGGCTACCTGGACGCGCCGGTTTCGGGCGGCGTGAAGCGGGCGGTCAACGGCACGCTCG
CCATCCTCGTCGGCGGCGCGGCGGACCTCCTCGCCCGCTGGCGGCCGGTGCTGGAGGCGATGGGCACCTCCGTCCTGCAT
ATCGGCCCGGCGGGATCGGGCCACGCCGCCAAGGCGCTGAACAACTTCGTCTCCGCCGCGGGCCTGCTCGCCACCGTCGA
GGCGCTCCACGTGGCCCAGCGCTTCGGCATCGCGCCGGAGGCCATGACCGACGTGCTCAACGCCTCGTCCGGGCGCAGCA
ACACCTCCGAGAACAAGGTCAAGCAGTTCATGCTCAGCGGCAGCTTCGCCTCGGGTTTCTCGCTGAAGCTGATGGACAAG
GATCTCGGCATCGCCGGGGCGCTGGCCGAGTCGGTCGACTACCCGCTCGGCTTCGGCCGCCACTGCATCGAGACCTGGCA
CGCGATCGCCGGGGAGGTCGGGCCCGCGGCCGACCACACCGAGATGTACCGCCTGCTGGCCAAGGGAGCCTGA

Upstream 100 bases:

>100_bases
GAGAACACCTTCCTCACCCCCACGGACTTCTCCGCCCTGCGCTGACGGGGACACGCGGCGTCGGGCCGCTCCGGCTGTCG
CCCAGAGGGACATTCACGGC

Downstream 100 bases:

>100_bases
GACGATGGCGCTCCCGGACCTGAGCCGCTTCTACATCGACGGCGCCTGGGTCGAGCCGGTCGCGGCCCGGCCGTTCCCGC
TGATCGATCCCGCCACCGAG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MEQRTIGVIGLGMMGDPMSRCLAKAGHVLRLLDADVGRTDALARDLGATALTRENAAGLDLLITMLPNSAIVEAVLLDDG
WADALPAGATVIDMSSSEPVRSRALGETLAGKGLGYLDAPVSGGVKRAVNGTLAILVGGAADLLARWRPVLEAMGTSVLH
IGPAGSGHAAKALNNFVSAAGLLATVEALHVAQRFGIAPEAMTDVLNASSGRSNTSENKVKQFMLSGSFASGFSLKLMDK
DLGIAGALAESVDYPLGFGRHCIETWHAIAGEVGPAADHTEMYRLLAKGA

Sequences:

>Translated_290_residues
MEQRTIGVIGLGMMGDPMSRCLAKAGHVLRLLDADVGRTDALARDLGATALTRENAAGLDLLITMLPNSAIVEAVLLDDG
WADALPAGATVIDMSSSEPVRSRALGETLAGKGLGYLDAPVSGGVKRAVNGTLAILVGGAADLLARWRPVLEAMGTSVLH
IGPAGSGHAAKALNNFVSAAGLLATVEALHVAQRFGIAPEAMTDVLNASSGRSNTSENKVKQFMLSGSFASGFSLKLMDK
DLGIAGALAESVDYPLGFGRHCIETWHAIAGEVGPAADHTEMYRLLAKGA
>Mature_290_residues
MEQRTIGVIGLGMMGDPMSRCLAKAGHVLRLLDADVGRTDALARDLGATALTRENAAGLDLLITMLPNSAIVEAVLLDDG
WADALPAGATVIDMSSSEPVRSRALGETLAGKGLGYLDAPVSGGVKRAVNGTLAILVGGAADLLARWRPVLEAMGTSVLH
IGPAGSGHAAKALNNFVSAAGLLATVEALHVAQRFGIAPEAMTDVLNASSGRSNTSENKVKQFMLSGSFASGFSLKLMDK
DLGIAGALAESVDYPLGFGRHCIETWHAIAGEVGPAADHTEMYRLLAKGA

Specific function: Catalyzes the conversion of 2-formylglutarate to (S)-2- hydroxymethylglutarate. Has very low activity with (S)-3- hydroxyisobutyrate [H]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=33.5616438356164, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI40556376, Length=255, Percent_Identity=27.4509803921569, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI145693186, Length=291, Percent_Identity=35.0515463917526, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1786719, Length=284, Percent_Identity=32.3943661971831, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1790315, Length=268, Percent_Identity=34.7014925373134, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789092, Length=258, Percent_Identity=31.3953488372093, Blast_Score=88, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17557316, Length=292, Percent_Identity=29.4520547945205, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24655230, Length=293, Percent_Identity=36.1774744027304, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI19922568, Length=293, Percent_Identity=36.1774744027304, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI28574115, Length=266, Percent_Identity=22.5563909774436, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.291 [H]

Molecular weight: Translated: 29823; Mature: 29823

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQRTIGVIGLGMMGDPMSRCLAKAGHVLRLLDADVGRTDALARDLGATALTRENAAGLD
CCCCEEEEEECCCCCCHHHHHHHHHCCEEEEHHCCCCCHHHHHHHHCCHHHHCCCCCCHH
LLITMLPNSAIVEAVLLDDGWADALPAGATVIDMSSSEPVRSRALGETLAGKGLGYLDAP
HHEECCCCHHHEEEEEECCCCHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC
VSGGVKRAVNGTLAILVGGAADLLARWRPVLEAMGTSVLHIGPAGSGHAAKALNNFVSAA
CCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHH
GLLATVEALHVAQRFGIAPEAMTDVLNASSGRSNTSENKVKQFMLSGSFASGFSLKLMDK
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECC
DLGIAGALAESVDYPLGFGRHCIETWHAIAGEVGPAADHTEMYRLLAKGA
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MEQRTIGVIGLGMMGDPMSRCLAKAGHVLRLLDADVGRTDALARDLGATALTRENAAGLD
CCCCEEEEEECCCCCCHHHHHHHHHCCEEEEHHCCCCCHHHHHHHHCCHHHHCCCCCCHH
LLITMLPNSAIVEAVLLDDGWADALPAGATVIDMSSSEPVRSRALGETLAGKGLGYLDAP
HHEECCCCHHHEEEEEECCCCHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC
VSGGVKRAVNGTLAILVGGAADLLARWRPVLEAMGTSVLHIGPAGSGHAAKALNNFVSAA
CCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHH
GLLATVEALHVAQRFGIAPEAMTDVLNASSGRSNTSENKVKQFMLSGSFASGFSLKLMDK
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECC
DLGIAGALAESVDYPLGFGRHCIETWHAIAGEVGPAADHTEMYRLLAKGA
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA