Click here to switch to the map view.

The map label for this gene is proP [H]

Identifier: 170749654

GI number: 170749654

Start: 3444362

End: 3445738

Strand: Reverse

Name: proP [H]

Synonym: Mrad2831_3251

Alternate gene names: 170749654

Gene position: 3445738-3444362 (Counterclockwise)

Preceding gene: 170749655

Following gene: 170749653

Centisome position: 56.69

GC content: 66.74

Gene sequence:

>1377_bases
ATGTCGGTCACGTCGGCGGCCTTGGCAACGGACGCAGCGGGCGCGCGCCCGGCCTCGTCCCAGTCGATCGAGGAGAAGCG
GAAGAGCGCCATCCGCGGCGCGTTCTTCTCCGAATACATCGACATGTTCGACATCTACCTGCCGGTGGTCGCGCTGGCGC
CGGTGATGTTCATGTTCCAGCCGAAGAACCTGTCTCCCCAGATGGAGACGGTGCTGGCCTCGCTGGTGTTCATCACAACC
CTGCTCGGCCGCCCGATCGGCGCGCTCATCTTCGGCCTGATCGCCGACAAGGTCGGCCGCCGGGCCGCCTCGATCTACTC
GGTGGCGGGCTTCGGGGTGATCACCCTGCTGATCGGCCTGCTGCCCGGTTACGAGACGCTCGGCCTCGCCTCGTACATCC
TGCTGGTGCTGCTGCGCTTCCTCGACGGCATCTTCCTCGGGGGCGGCTACACGGGGGCGATGCCGCTCGCCATCGAGTAT
TCAAAGAAGGACAAGCGCGGCTTCGTCGGCGGCCTGATCATCGCGGGCTTCCCGGCCGCCTATGTCAGCATCAACCTCAT
CACGATGCTGATGTTCTACCTGTTCCCGCTCGACGGGCCCGGGTCGCCCTACACGGTCTGGGGCTGGCGCATCCCCTTCA
TCGTCGGCGCGGCGCTCGCCGGCCTGCTCGCCCTCTACTACGTCCACAAGGTCTCGGAATCGGAGGTCTGGAAGAAGGAG
GCCGCCGCCGGGGCCGAACAGGCCGAGGTGTCGCCGATGGCGGGCCTGTTCACCGGGAAGGGCGGGCGCGACCTCCTGCA
GGTCCTGCTGCTGATGACCGGCTTCTGGACGACGCAGAACATCATCACGATCTACATGCCGACCGGTCTGCTGGTGAAGA
CCCTGCACCTCGACGGCTTCGCGCTGACCGCGACGCTGATGATCTGCTACACGATCCTGTTCTTCAGCTACATCGGCTCC
GGCCTGCTGGGGCAGGTCATCGGCCGCCGGCGGTTCTTCGTGATCGTCGGCCCGCTGATCGCGACGGTCGGCGCGTACAT
CCTCTACACGCTGACCGTCACGCCCGGGATGGCGTTCGGCACCCGCGTGCTGCTGGTCAGCCTCCTGGCGGTCATCGTCA
CCTCGCCCTGGGGCGTCATCGTCACCTACATCAACGAGCGCTTCGCCACGGATGTCCGCGCCACCGGCTTCGGCATCGGC
TTCAGCCTCTCGGTGGTGATCCCGTCCTTCTACGCCTTCTACATGGACTGGCTGAGCGCGCTGATGCCGCTCGCGCTGAC
GCCCGTGGCCCTGCTGATCGTCGGCGGCCTGATCGGCACCGTGGGCGCGCTCATCGGGCCGGAGACCAAGGACGTCGATT
TCGGCACCGGCCACTGA

Upstream 100 bases:

>100_bases
GGCGCTGATCCCGCGGACGACCGCGCGGGCTCGGCGCCCGCGCCCACGCTCCGACGCAGTCAAAAAAGTTGCGCGGCAGT
ACAACAGGGAGGGAGACGCC

Downstream 100 bases:

>100_bases
GCCGGACGCCGCGCGGAGGACGCAGCATGAACGGGAACCGGATCGGCTTCATCGGGCTCGGCAACATGGGCGGCCGCATG
ACGCGCAGCCTGACCGGGGC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSVTSAALATDAAGARPASSQSIEEKRKSAIRGAFFSEYIDMFDIYLPVVALAPVMFMFQPKNLSPQMETVLASLVFITT
LLGRPIGALIFGLIADKVGRRAASIYSVAGFGVITLLIGLLPGYETLGLASYILLVLLRFLDGIFLGGGYTGAMPLAIEY
SKKDKRGFVGGLIIAGFPAAYVSINLITMLMFYLFPLDGPGSPYTVWGWRIPFIVGAALAGLLALYYVHKVSESEVWKKE
AAAGAEQAEVSPMAGLFTGKGGRDLLQVLLLMTGFWTTQNIITIYMPTGLLVKTLHLDGFALTATLMICYTILFFSYIGS
GLLGQVIGRRRFFVIVGPLIATVGAYILYTLTVTPGMAFGTRVLLVSLLAVIVTSPWGVIVTYINERFATDVRATGFGIG
FSLSVVIPSFYAFYMDWLSALMPLALTPVALLIVGGLIGTVGALIGPETKDVDFGTGH

Sequences:

>Translated_458_residues
MSVTSAALATDAAGARPASSQSIEEKRKSAIRGAFFSEYIDMFDIYLPVVALAPVMFMFQPKNLSPQMETVLASLVFITT
LLGRPIGALIFGLIADKVGRRAASIYSVAGFGVITLLIGLLPGYETLGLASYILLVLLRFLDGIFLGGGYTGAMPLAIEY
SKKDKRGFVGGLIIAGFPAAYVSINLITMLMFYLFPLDGPGSPYTVWGWRIPFIVGAALAGLLALYYVHKVSESEVWKKE
AAAGAEQAEVSPMAGLFTGKGGRDLLQVLLLMTGFWTTQNIITIYMPTGLLVKTLHLDGFALTATLMICYTILFFSYIGS
GLLGQVIGRRRFFVIVGPLIATVGAYILYTLTVTPGMAFGTRVLLVSLLAVIVTSPWGVIVTYINERFATDVRATGFGIG
FSLSVVIPSFYAFYMDWLSALMPLALTPVALLIVGGLIGTVGALIGPETKDVDFGTGH
>Mature_457_residues
SVTSAALATDAAGARPASSQSIEEKRKSAIRGAFFSEYIDMFDIYLPVVALAPVMFMFQPKNLSPQMETVLASLVFITTL
LGRPIGALIFGLIADKVGRRAASIYSVAGFGVITLLIGLLPGYETLGLASYILLVLLRFLDGIFLGGGYTGAMPLAIEYS
KKDKRGFVGGLIIAGFPAAYVSINLITMLMFYLFPLDGPGSPYTVWGWRIPFIVGAALAGLLALYYVHKVSESEVWKKEA
AAGAEQAEVSPMAGLFTGKGGRDLLQVLLLMTGFWTTQNIITIYMPTGLLVKTLHLDGFALTATLMICYTILFFSYIGSG
LLGQVIGRRRFFVIVGPLIATVGAYILYTLTVTPGMAFGTRVLLVSLLAVIVTSPWGVIVTYINERFATDVRATGFGIGF
SLSVVIPSFYAFYMDWLSALMPLALTPVALLIVGGLIGTVGALIGPETKDVDFGTGH

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=445, Percent_Identity=27.8651685393258, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788292, Length=410, Percent_Identity=27.0731707317073, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789941, Length=412, Percent_Identity=28.8834951456311, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788942, Length=418, Percent_Identity=27.9904306220096, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786229, Length=351, Percent_Identity=27.6353276353276, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082231, Length=481, Percent_Identity=25.3638253638254, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49013; Mature: 48882

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTSAALATDAAGARPASSQSIEEKRKSAIRGAFFSEYIDMFDIYLPVVALAPVMFMFQ
CCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKNLSPQMETVLASLVFITTLLGRPIGALIFGLIADKVGRRAASIYSVAGFGVITLLIGL
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPGYETLGLASYILLVLLRFLDGIFLGGGYTGAMPLAIEYSKKDKRGFVGGLIIAGFPAA
CCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHCCHHH
YVSINLITMLMFYLFPLDGPGSPYTVWGWRIPFIVGAALAGLLALYYVHKVSESEVWKKE
HHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AAAGAEQAEVSPMAGLFTGKGGRDLLQVLLLMTGFWTTQNIITIYMPTGLLVKTLHLDGF
HHCCCCHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCCCH
ALTATLMICYTILFFSYIGSGLLGQVIGRRRFFVIVGPLIATVGAYILYTLTVTPGMAFG
HHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHH
TRVLLVSLLAVIVTSPWGVIVTYINERFATDVRATGFGIGFSLSVVIPSFYAFYMDWLSA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LMPLALTPVALLIVGGLIGTVGALIGPETKDVDFGTGH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SVTSAALATDAAGARPASSQSIEEKRKSAIRGAFFSEYIDMFDIYLPVVALAPVMFMFQ
CCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKNLSPQMETVLASLVFITTLLGRPIGALIFGLIADKVGRRAASIYSVAGFGVITLLIGL
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPGYETLGLASYILLVLLRFLDGIFLGGGYTGAMPLAIEYSKKDKRGFVGGLIIAGFPAA
CCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHCCHHH
YVSINLITMLMFYLFPLDGPGSPYTVWGWRIPFIVGAALAGLLALYYVHKVSESEVWKKE
HHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AAAGAEQAEVSPMAGLFTGKGGRDLLQVLLLMTGFWTTQNIITIYMPTGLLVKTLHLDGF
HHCCCCHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCCCH
ALTATLMICYTILFFSYIGSGLLGQVIGRRRFFVIVGPLIATVGAYILYTLTVTPGMAFG
HHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHH
TRVLLVSLLAVIVTSPWGVIVTYINERFATDVRATGFGIGFSLSVVIPSFYAFYMDWLSA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LMPLALTPVALLIVGGLIGTVGALIGPETKDVDFGTGH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]