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The map label for this gene is iaaA [H]

Identifier: 170749648

GI number: 170749648

Start: 3439297

End: 3440274

Strand: Reverse

Name: iaaA [H]

Synonym: Mrad2831_3245

Alternate gene names: 170749648

Gene position: 3440274-3439297 (Counterclockwise)

Preceding gene: 170749649

Following gene: 170749647

Centisome position: 56.6

GC content: 76.18

Gene sequence:

>978_bases
ATGAGCGACCACCGCCCCGTTCTCGTGATCCACGGCGGCGCCGGGACGATCGCGCAGAATCAGGATGATCCGGGCGAGGC
GCGCTACCACGCGGCCCTCGCGGACATCCTGGCGGCCGGAGCGGCCTGCCTCGCCGACGGCGGCAGCGCCCTCGACGCCG
TGAGCGTCGCCGTCGACCTCCTCGAAGAGTGCCCGCTCTTCAACGCCGGCCACGGGGCCGTCTTCACCAGCGCCGAGACC
CACGAGCTCGACGCCGCGCTCATGGACGGCGCCACCCTGCGGGCCGGCGCCGTCGCGGGCGTCGCGCGGGTGCGCCGGCC
CGGCCGCGCGGCCCGCGCGGTGATGGAGGCGGGCGAGCACGTCCTGATGGCCGGGGCCGGCGCCGAGGCCTTCGCGCGGC
AGCACGGCCTGGAGATGGTCGAGCCGGACTTCTTCTCGACGGAGGCCCGCCGCGACCAGCTCCGCCGGGCCCGGGCCGCC
GGACAGGTCGCCCTCGACCACGACGCGGCGTCGGGCCCCCTCGACGAGACCCGCAAGTTCGGCACGGTGGGCGCCGTCGC
CCTCGACCGGGACGGCCATCTCGCCGCCCTGACCTCGACCGGCGGCATGACCAACAAGCGTCCGGGCCGGATCGGCGACT
CGCCGCTGATCGGCGCGGGCACCTACGCGGACGACCGGACCGCCGCAATCTCCTGCACCGGTACGGGGGAAGCCTTCATC
CGCGTCGCGGCGGCGCACGATGTCTGCGCGCGCATGGCCTACGGGGGCCAGGACCTCGCGGCGGCGGCTCGGGCGGTGGT
CGAGGACGCTCTCCCGGCGGTCGGGGGCCGCGGCGGATTGATCGCCGTCGACGCGCGCGGCCGGGTCGCGATGCCGTTCA
ACACCGAGGGCATGTATCGCGGTGTCGTCCGCCCCGGCGAGGCGCCAACCACCGCGATCTTCGGCGAATCCCGCCGCGTC
CCGGCTCCCGCGCGCTGA

Upstream 100 bases:

>100_bases
CGGAAGCGGTGCTGCCCTGGCTCGACCGCGACTACGCCGCCTGGCCGCCGCAGGGCGGAGCCGACGACCTGTCCGACACA
CCGCCTCCGGGAACCGTTCC

Downstream 100 bases:

>100_bases
GATCCGCCATGCCGACCATCCCAGCCGGAACCGGCTCTCCCACCGCGATGCCCGACGGGCGGGTCGTCTCCGTCAGCGGC
CTCACCGTCCGCTTCGCGAC

Product: peptidase T2 asparaginase 2

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSDHRPVLVIHGGAGTIAQNQDDPGEARYHAALADILAAGAACLADGGSALDAVSVAVDLLEECPLFNAGHGAVFTSAET
HELDAALMDGATLRAGAVAGVARVRRPGRAARAVMEAGEHVLMAGAGAEAFARQHGLEMVEPDFFSTEARRDQLRRARAA
GQVALDHDAASGPLDETRKFGTVGAVALDRDGHLAALTSTGGMTNKRPGRIGDSPLIGAGTYADDRTAAISCTGTGEAFI
RVAAAHDVCARMAYGGQDLAAAARAVVEDALPAVGGRGGLIAVDARGRVAMPFNTEGMYRGVVRPGEAPTTAIFGESRRV
PAPAR

Sequences:

>Translated_325_residues
MSDHRPVLVIHGGAGTIAQNQDDPGEARYHAALADILAAGAACLADGGSALDAVSVAVDLLEECPLFNAGHGAVFTSAET
HELDAALMDGATLRAGAVAGVARVRRPGRAARAVMEAGEHVLMAGAGAEAFARQHGLEMVEPDFFSTEARRDQLRRARAA
GQVALDHDAASGPLDETRKFGTVGAVALDRDGHLAALTSTGGMTNKRPGRIGDSPLIGAGTYADDRTAAISCTGTGEAFI
RVAAAHDVCARMAYGGQDLAAAARAVVEDALPAVGGRGGLIAVDARGRVAMPFNTEGMYRGVVRPGEAPTTAIFGESRRV
PAPAR
>Mature_324_residues
SDHRPVLVIHGGAGTIAQNQDDPGEARYHAALADILAAGAACLADGGSALDAVSVAVDLLEECPLFNAGHGAVFTSAETH
ELDAALMDGATLRAGAVAGVARVRRPGRAARAVMEAGEHVLMAGAGAEAFARQHGLEMVEPDFFSTEARRDQLRRARAAG
QVALDHDAASGPLDETRKFGTVGAVALDRDGHLAALTSTGGMTNKRPGRIGDSPLIGAGTYADDRTAAISCTGTGEAFIR
VAAAHDVCARMAYGGQDLAAAARAVVEDALPAVGGRGGLIAVDARGRVAMPFNTEGMYRGVVRPGEAPTTAIFGESRRVP
APAR

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=304, Percent_Identity=36.1842105263158, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI145275200, Length=304, Percent_Identity=36.1842105263158, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI98991769, Length=267, Percent_Identity=27.3408239700375, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI285002251, Length=248, Percent_Identity=32.6612903225806, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787050, Length=314, Percent_Identity=59.8726114649682, Blast_Score=350, Evalue=8e-98,
Organism=Caenorhabditis elegans, GI17569239, Length=240, Percent_Identity=28.3333333333333, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32564649, Length=345, Percent_Identity=23.1884057971014, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI18921183, Length=316, Percent_Identity=37.9746835443038, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21357893, Length=265, Percent_Identity=30.188679245283, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24652135, Length=183, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI221330102, Length=183, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33191; Mature: 33059

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHRPVLVIHGGAGTIAQNQDDPGEARYHAALADILAAGAACLADGGSALDAVSVAVDL
CCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHH
LEECPLFNAGHGAVFTSAETHELDAALMDGATLRAGAVAGVARVRRPGRAARAVMEAGEH
HHHCCCCCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCE
VLMAGAGAEAFARQHGLEMVEPDFFSTEARRDQLRRARAAGQVALDHDAASGPLDETRKF
EEEECCCHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHC
GTVGAVALDRDGHLAALTSTGGMTNKRPGRIGDSPLIGAGTYADDRTAAISCTGTGEAFI
CCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCEEE
RVAAAHDVCARMAYGGQDLAAAARAVVEDALPAVGGRGGLIAVDARGRVAMPFNTEGMYR
EEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCEE
GVVRPGEAPTTAIFGESRRVPAPAR
CCCCCCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
SDHRPVLVIHGGAGTIAQNQDDPGEARYHAALADILAAGAACLADGGSALDAVSVAVDL
CCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHH
LEECPLFNAGHGAVFTSAETHELDAALMDGATLRAGAVAGVARVRRPGRAARAVMEAGEH
HHHCCCCCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCE
VLMAGAGAEAFARQHGLEMVEPDFFSTEARRDQLRRARAAGQVALDHDAASGPLDETRKF
EEEECCCHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHC
GTVGAVALDRDGHLAALTSTGGMTNKRPGRIGDSPLIGAGTYADDRTAAISCTGTGEAFI
CCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCEEE
RVAAAHDVCARMAYGGQDLAAAARAVVEDALPAVGGRGGLIAVDARGRVAMPFNTEGMYR
EEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCEE
GVVRPGEAPTTAIFGESRRVPAPAR
CCCCCCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]