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The map label for this gene is asnB [H]

Identifier: 170749593

GI number: 170749593

Start: 3366185

End: 3367954

Strand: Reverse

Name: asnB [H]

Synonym: Mrad2831_3188

Alternate gene names: 170749593

Gene position: 3367954-3366185 (Counterclockwise)

Preceding gene: 170749595

Following gene: 170749592

Centisome position: 55.41

GC content: 68.76

Gene sequence:

>1770_bases
ATGTGTGGAATCTGCGGCGAGATCTGTTTCGACAGGCCGGCGGACCCCGCCGCCGTCGACGCGATGATGGGATGCCTGCA
ACCGCGCGGGCCGGACGCCGCGGGGCTTCTCGGTTCCGGCCGGGTCGTCTTCGGCCATCGCAGGCTCAAGATCATCGACC
TGTCCGAGGCCGGCCAGCAACCCATGGTCGATCCGGCGCTCGGGCTCGCGATCGTGTTCAACGGCTGCATCTACAATTTC
CGCGCGCTGCGCGCCGAACTCGAGGCGGCGGGCTACAGCTTCTTCTCGGCCAGCGACACAGAGGTGGTGCTCAAGGCCTA
TCATGCCTGGGGCCGTGCCTGCGTCGAGCGCTTCGCCGGCATGTTCGCGTTCGCCATCCTGGAGCGGGAATCCGGGCGGG
TCGTGCTGGCGCGCGACCGGCTGGGCGTGAAGCCGCTCTACTACAGCGAGACGAAAACCCGGTTCCGCTTCGCCTCGTCG
CTCCCGGCCCTGCTCGCGGCCGGCGACATCGATACCGCGATCGATCCGGTCGCCCTCCACCATTACATGAGCTGGCACGC
GGGCGTGCCGGCCCCGCTCACCATTCTCAAGGGTGTGCGCAAGCTGGCACCGGCGACGATCCTGACGATCGAGCAGGACG
GGACGCAGCACGCGTCGACCTACTGGACACTCGCGATCGGACCCCGCGAGGCCGACCGCGGCCGGACCGAGGCGGACTGG
CGCGAGGCCGTTCTCGATGCCCTGGGCACCGCCGTCGAGCGCAGGCAGGTCGCCGACGTCCCGACGGGCGTCCTGCTCTC
GGGTGGCCTGGACAGCTCGCTGCTCGTGGCCCTGCTCGCGCGGAGCGGGCAGCAGGGCCTGAAGACCTTCTCGGTAGGCT
TCGACGCGGTGAACGGCGTCGAGGGCGACGAGTTCAAGTACTCGGATATCGTCGCCGAGACCTTCGCCACGGACCACACG
AAGCTGGCGGTCGACGGCTCCCGGACGCTGGAAGCGCTGCCCGCGGCGATCGCGGCGATGTCGGAACCGCAGATGAGCCA
CGACGCGGTGGCCTTCCTCCTGCTCTCGCAGGAGGTCGCCAAGCACGTGACGGTGATCCAGAGCGGGCAGGGCGCCGACG
AGGTGTTCGGCGGCTATCACTGGTACCCCAAGCTGATGGGCTCGACCGACCCGGTCGCCGATTACGCGAAAGCGTATTTC
GACCGCGACCACGCCGAGATGCGCGAGGCTCTGACGCCCGACATGCTGGGGGGCGATCACAGCACCGACTACATCGCCGC
CTTCTTCGCGAAGTCGCGGAGCGCCAGCGCCATCGACAAGACCCTGCAGCTCGACCAGGAGGTCATGATGGTCGACGACC
CGGTCAAGCGGGTCGACAACATGACGATGGCCTGCGGCCTCGAGGCGCGGGTGCCGTTCCTCGACCACGACCTCGTCGAG
CTGGCGGCCCGCATCCCGGCGGAGCTCAAGGTCCGGGACGGCGGCAAGTACATCCTGAAGGAAGCCGCGCGGGCGGTTGT
CCCGGCCGAGGTGATCGACCGCAAGAAGGGGTATTTCCCCGTGCCCGCCCTGAAGCACATCCGCGGCCCGTTCCTCGACT
ACGTCCGCGAGATCCTCGACCGGCCCGAGGCGCGCGCGCGCGGCATCTTCAACCGCGCCTACGTCGATCACCTGCTCGCC
GATCCCGACGGCACGCTGACGCCCAAGGGCAACTCGAAGCTCTGGCAGGTGGCCCTTCTGGAGAGCTGGCTCCAGACCCA
CGGCGTCTGA

Upstream 100 bases:

>100_bases
CTGGCGCAGCGCCGTGGCCGGATCGGCGGAACGCCGCGGCGCGCGGGCCGTTTCGCTCGACGGAGCGTGCGTCGCGCCCG
ACCGAGACCCTGCTGACATC

Downstream 100 bases:

>100_bases
GACGGGCCGCGGCACGGCTTTCGCGACAGATCGTGATCGACCGCGCGCTCAAGGTGCCGCGCTTTGTCCACGATCGTCCC
GGATCAGCCGGCCGACAGAC

Product: asparagine synthase amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MCGICGEICFDRPADPAAVDAMMGCLQPRGPDAAGLLGSGRVVFGHRRLKIIDLSEAGQQPMVDPALGLAIVFNGCIYNF
RALRAELEAAGYSFFSASDTEVVLKAYHAWGRACVERFAGMFAFAILERESGRVVLARDRLGVKPLYYSETKTRFRFASS
LPALLAAGDIDTAIDPVALHHYMSWHAGVPAPLTILKGVRKLAPATILTIEQDGTQHASTYWTLAIGPREADRGRTEADW
REAVLDALGTAVERRQVADVPTGVLLSGGLDSSLLVALLARSGQQGLKTFSVGFDAVNGVEGDEFKYSDIVAETFATDHT
KLAVDGSRTLEALPAAIAAMSEPQMSHDAVAFLLLSQEVAKHVTVIQSGQGADEVFGGYHWYPKLMGSTDPVADYAKAYF
DRDHAEMREALTPDMLGGDHSTDYIAAFFAKSRSASAIDKTLQLDQEVMMVDDPVKRVDNMTMACGLEARVPFLDHDLVE
LAARIPAELKVRDGGKYILKEAARAVVPAEVIDRKKGYFPVPALKHIRGPFLDYVREILDRPEARARGIFNRAYVDHLLA
DPDGTLTPKGNSKLWQVALLESWLQTHGV

Sequences:

>Translated_589_residues
MCGICGEICFDRPADPAAVDAMMGCLQPRGPDAAGLLGSGRVVFGHRRLKIIDLSEAGQQPMVDPALGLAIVFNGCIYNF
RALRAELEAAGYSFFSASDTEVVLKAYHAWGRACVERFAGMFAFAILERESGRVVLARDRLGVKPLYYSETKTRFRFASS
LPALLAAGDIDTAIDPVALHHYMSWHAGVPAPLTILKGVRKLAPATILTIEQDGTQHASTYWTLAIGPREADRGRTEADW
REAVLDALGTAVERRQVADVPTGVLLSGGLDSSLLVALLARSGQQGLKTFSVGFDAVNGVEGDEFKYSDIVAETFATDHT
KLAVDGSRTLEALPAAIAAMSEPQMSHDAVAFLLLSQEVAKHVTVIQSGQGADEVFGGYHWYPKLMGSTDPVADYAKAYF
DRDHAEMREALTPDMLGGDHSTDYIAAFFAKSRSASAIDKTLQLDQEVMMVDDPVKRVDNMTMACGLEARVPFLDHDLVE
LAARIPAELKVRDGGKYILKEAARAVVPAEVIDRKKGYFPVPALKHIRGPFLDYVREILDRPEARARGIFNRAYVDHLLA
DPDGTLTPKGNSKLWQVALLESWLQTHGV
>Mature_589_residues
MCGICGEICFDRPADPAAVDAMMGCLQPRGPDAAGLLGSGRVVFGHRRLKIIDLSEAGQQPMVDPALGLAIVFNGCIYNF
RALRAELEAAGYSFFSASDTEVVLKAYHAWGRACVERFAGMFAFAILERESGRVVLARDRLGVKPLYYSETKTRFRFASS
LPALLAAGDIDTAIDPVALHHYMSWHAGVPAPLTILKGVRKLAPATILTIEQDGTQHASTYWTLAIGPREADRGRTEADW
REAVLDALGTAVERRQVADVPTGVLLSGGLDSSLLVALLARSGQQGLKTFSVGFDAVNGVEGDEFKYSDIVAETFATDHT
KLAVDGSRTLEALPAAIAAMSEPQMSHDAVAFLLLSQEVAKHVTVIQSGQGADEVFGGYHWYPKLMGSTDPVADYAKAYF
DRDHAEMREALTPDMLGGDHSTDYIAAFFAKSRSASAIDKTLQLDQEVMMVDDPVKRVDNMTMACGLEARVPFLDHDLVE
LAARIPAELKVRDGGKYILKEAARAVVPAEVIDRKKGYFPVPALKHIRGPFLDYVREILDRPEARARGIFNRAYVDHLLA
DPDGTLTPKGNSKLWQVALLESWLQTHGV

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=546, Percent_Identity=27.8388278388278, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17560178, Length=531, Percent_Identity=25.4237288135593, Blast_Score=121, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6325403, Length=549, Percent_Identity=26.5938069216758, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6321563, Length=616, Percent_Identity=25.3246753246753, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45553209, Length=560, Percent_Identity=25.5357142857143, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 64088; Mature: 64088

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGICGEICFDRPADPAAVDAMMGCLQPRGPDAAGLLGSGRVVFGHRRLKIIDLSEAGQQ
CCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCEEEEEEHHCCCCC
PMVDPALGLAIVFNGCIYNFRALRAELEAAGYSFFSASDTEVVLKAYHAWGRACVERFAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
MFAFAILERESGRVVLARDRLGVKPLYYSETKTRFRFASSLPALLAAGDIDTAIDPVALH
HHHHHHHCCCCCCEEEEECCCCCCCEEECCHHHHHHHHHCCCHHHCCCCCCCCCCHHHHH
HYMSWHAGVPAPLTILKGVRKLAPATILTIEQDGTQHASTYWTLAIGPREADRGRTEADW
HHHHHCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCHHH
REAVLDALGTAVERRQVADVPTGVLLSGGLDSSLLVALLARSGQQGLKTFSVGFDAVNGV
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHCCCHHHCCC
EGDEFKYSDIVAETFATDHTKLAVDGSRTLEALPAAIAAMSEPQMSHDAVAFLLLSQEVA
CCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KHVTVIQSGQGADEVFGGYHWYPKLMGSTDPVADYAKAYFDRDHAEMREALTPDMLGGDH
HHHHHHCCCCCHHHHCCCCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCC
STDYIAAFFAKSRSASAIDKTLQLDQEVMMVDDPVKRVDNMTMACGLEARVPFLDHDLVE
CHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCCCHHHHHCCCEEECCCCCCCCCCCHHHHH
LAARIPAELKVRDGGKYILKEAARAVVPAEVIDRKKGYFPVPALKHIRGPFLDYVREILD
HHHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHC
RPEARARGIFNRAYVDHLLADPDGTLTPKGNSKLWQVALLESWLQTHGV
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGICGEICFDRPADPAAVDAMMGCLQPRGPDAAGLLGSGRVVFGHRRLKIIDLSEAGQQ
CCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCEEEEEEHHCCCCC
PMVDPALGLAIVFNGCIYNFRALRAELEAAGYSFFSASDTEVVLKAYHAWGRACVERFAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
MFAFAILERESGRVVLARDRLGVKPLYYSETKTRFRFASSLPALLAAGDIDTAIDPVALH
HHHHHHHCCCCCCEEEEECCCCCCCEEECCHHHHHHHHHCCCHHHCCCCCCCCCCHHHHH
HYMSWHAGVPAPLTILKGVRKLAPATILTIEQDGTQHASTYWTLAIGPREADRGRTEADW
HHHHHCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCHHH
REAVLDALGTAVERRQVADVPTGVLLSGGLDSSLLVALLARSGQQGLKTFSVGFDAVNGV
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHCCCHHHCCC
EGDEFKYSDIVAETFATDHTKLAVDGSRTLEALPAAIAAMSEPQMSHDAVAFLLLSQEVA
CCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KHVTVIQSGQGADEVFGGYHWYPKLMGSTDPVADYAKAYFDRDHAEMREALTPDMLGGDH
HHHHHHCCCCCHHHHCCCCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCC
STDYIAAFFAKSRSASAIDKTLQLDQEVMMVDDPVKRVDNMTMACGLEARVPFLDHDLVE
CHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCCCHHHHHCCCEEECCCCCCCCCCCHHHHH
LAARIPAELKVRDGGKYILKEAARAVVPAEVIDRKKGYFPVPALKHIRGPFLDYVREILD
HHHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHC
RPEARARGIFNRAYVDHLLADPDGTLTPKGNSKLWQVALLESWLQTHGV
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]