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The map label for this gene is ybdK [C]

Identifier: 170749478

GI number: 170749478

Start: 3245625

End: 3246791

Strand: Reverse

Name: ybdK [C]

Synonym: Mrad2831_3073

Alternate gene names: 170749478

Gene position: 3246791-3245625 (Counterclockwise)

Preceding gene: 170749479

Following gene: 170749477

Centisome position: 53.42

GC content: 74.04

Gene sequence:

>1167_bases
ATGGCCCACGCCTACAGGTTCGGCATCGAGGAGGAGTTCTTCCTGGCCGATGCCCGCACGCGCGGCACACCGCGCGCCGG
CCTCCGCGCCTTCCACGCCGCCGTGCGGGGCCGCCTCGACGATGCCGAGCGCGAGCAGCTCCAGTGCCAGGTGGAGGTGG
CCTCGCCGCCGACCGCCGACCTCGCGGAGGCGCGGGCGCATCTCGCGGACCTGCGCGCGAGCCTCGCGGAGATCGGCGGC
GAGCGGGGCATCCTGACCTTCGCGGCGGGCACGCATCCGACCGCCCGGTGGCGCGACCAGCGGCCCACCGACAAGGCGCG
CTATCACGGCATCATGGCCGACCTCCGGATGCTCGGCCGGCGCAACCTCGTCTGCGGCCTCCACGTCCACGTGGAGGTGC
CGGATCCCGCGGCACGGATCGGGCTGATCAACCGGCTTCTGCCCTACCTGCCGGTGCTGCTCGCGCTCTCGACCGGGTCA
CCGTTCTGGCAGGGGCAGCAGACCGGTCTCGCCGGCTACCGGATGCGCGCCTACGCCGAGCTGCCGCGCACCGGCGTTCC
CGAGCTGTTCGCCGATGCCGACGACTACGCCCGCTACGTCGACGTGATGACCCGCTCGGGTGCCATCGCGGATGCCAGCT
ATCTCTGGTGGCACGTGCGCGCGTCCGTGAAGTACCCGACGCTAGAGCTGCGAATCGCCGACAGCTGCGCGCGCCTCGAC
GACGCGCTCTGCATCGCCGCGCTGTTCCGCTGCCTCGTTCGCCGGGTCGTCCGGGACAGGCGGCTCAATGCCGGCCTGAC
CGCGGCCTCACGCGGCTTCGTTTTGGAGAATCTCTGGCGGGCCGAGTGCGACGGCGTCCGCGCGACGCTCATCGACGAGG
CGCGGGTCCGGGCCGTGCCGGTCGCCGCAGCGGTCGAGGCCCTGCTCGCCGACATCGCCGAGGATGCCGATGCCCTGGGC
TGCGGCGCGGCCTGCGCGCACGCGTCGCGGATCGTCGCGGAGGGGACGAGCGCCGACCGGCAGATGGCCGTGTTCGCGGC
CGCGCGCCGGTCGGGCGCGCGGGAGCGCGCGGCGCTGTCGGCAGTGGTCGATCATCTCGCGGCGGAGACCGCCGCCGGCT
CGAAGTCCGGGCCTGGGGGGCACCCGAACATCGCTCCGGCGGCGTGA

Upstream 100 bases:

>100_bases
CTGGCGCTGAGGCTTTTCGCGCCGCCGGTCGGGCCGGAACCCGGCCGGTGCGGCGGCGTTCGCCGGACAACGATCGGCCC
GTCCGGGGCAGGGAGCGCGG

Downstream 100 bases:

>100_bases
CGACGGGCGCGGACACGGCTGAGGGGGGATCGTCGAGGCCGCGGTCCGTCGGCCGCGCTCGAGGACGCGGTCGCCCACAC
AGTCGCATGGCCATGTTGCC

Product: glutamate--cysteine ligase GCS2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MAHAYRFGIEEEFFLADARTRGTPRAGLRAFHAAVRGRLDDAEREQLQCQVEVASPPTADLAEARAHLADLRASLAEIGG
ERGILTFAAGTHPTARWRDQRPTDKARYHGIMADLRMLGRRNLVCGLHVHVEVPDPAARIGLINRLLPYLPVLLALSTGS
PFWQGQQTGLAGYRMRAYAELPRTGVPELFADADDYARYVDVMTRSGAIADASYLWWHVRASVKYPTLELRIADSCARLD
DALCIAALFRCLVRRVVRDRRLNAGLTAASRGFVLENLWRAECDGVRATLIDEARVRAVPVAAAVEALLADIAEDADALG
CGAACAHASRIVAEGTSADRQMAVFAAARRSGARERAALSAVVDHLAAETAAGSKSGPGGHPNIAPAA

Sequences:

>Translated_388_residues
MAHAYRFGIEEEFFLADARTRGTPRAGLRAFHAAVRGRLDDAEREQLQCQVEVASPPTADLAEARAHLADLRASLAEIGG
ERGILTFAAGTHPTARWRDQRPTDKARYHGIMADLRMLGRRNLVCGLHVHVEVPDPAARIGLINRLLPYLPVLLALSTGS
PFWQGQQTGLAGYRMRAYAELPRTGVPELFADADDYARYVDVMTRSGAIADASYLWWHVRASVKYPTLELRIADSCARLD
DALCIAALFRCLVRRVVRDRRLNAGLTAASRGFVLENLWRAECDGVRATLIDEARVRAVPVAAAVEALLADIAEDADALG
CGAACAHASRIVAEGTSADRQMAVFAAARRSGARERAALSAVVDHLAAETAAGSKSGPGGHPNIAPAA
>Mature_387_residues
AHAYRFGIEEEFFLADARTRGTPRAGLRAFHAAVRGRLDDAEREQLQCQVEVASPPTADLAEARAHLADLRASLAEIGGE
RGILTFAAGTHPTARWRDQRPTDKARYHGIMADLRMLGRRNLVCGLHVHVEVPDPAARIGLINRLLPYLPVLLALSTGSP
FWQGQQTGLAGYRMRAYAELPRTGVPELFADADDYARYVDVMTRSGAIADASYLWWHVRASVKYPTLELRIADSCARLDD
ALCIAALFRCLVRRVVRDRRLNAGLTAASRGFVLENLWRAECDGVRATLIDEARVRAVPVAAAVEALLADIAEDADALGC
GAACAHASRIVAEGTSADRQMAVFAAARRSGARERAALSAVVDHLAAETAAGSKSGPGGHPNIAPAA

Specific function: ATP-dependent carboxylate-amine ligase [H]

COG id: COG2170

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carboxylate-amine ligase family [H]

Homologues:

Organism=Escherichia coli, GI1786795, Length=318, Percent_Identity=22.0125786163522, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011793
- InterPro:   IPR006336 [H]

Pfam domain/function: PF04107 GCS2 [H]

EC number: NA

Molecular weight: Translated: 41814; Mature: 41683

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHAYRFGIEEEFFLADARTRGTPRAGLRAFHAAVRGRLDDAEREQLQCQVEVASPPTAD
CCCCEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHCCEEEEEEECCCCCHH
LAEARAHLADLRASLAEIGGERGILTFAAGTHPTARWRDQRPTDKARYHGIMADLRMLGR
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
RNLVCGLHVHVEVPDPAARIGLINRLLPYLPVLLALSTGSPFWQGQQTGLAGYRMRAYAE
CCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
LPRTGVPELFADADDYARYVDVMTRSGAIADASYLWWHVRASVKYPTLELRIADSCARLD
CCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCHHEEEEEEEEEEECCEEEEEECHHHHHHH
DALCIAALFRCLVRRVVRDRRLNAGLTAASRGFVLENLWRAECDGVRATLIDEARVRAVP
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHH
VAAAVEALLADIAEDADALGCGAACAHASRIVAEGTSADRQMAVFAAARRSGARERAALS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHH
AVVDHLAAETAAGSKSGPGGHPNIAPAA
HHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AHAYRFGIEEEFFLADARTRGTPRAGLRAFHAAVRGRLDDAEREQLQCQVEVASPPTAD
CCCEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHCCEEEEEEECCCCCHH
LAEARAHLADLRASLAEIGGERGILTFAAGTHPTARWRDQRPTDKARYHGIMADLRMLGR
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
RNLVCGLHVHVEVPDPAARIGLINRLLPYLPVLLALSTGSPFWQGQQTGLAGYRMRAYAE
CCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
LPRTGVPELFADADDYARYVDVMTRSGAIADASYLWWHVRASVKYPTLELRIADSCARLD
CCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCHHEEEEEEEEEEECCEEEEEECHHHHHHH
DALCIAALFRCLVRRVVRDRRLNAGLTAASRGFVLENLWRAECDGVRATLIDEARVRAVP
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHH
VAAAVEALLADIAEDADALGCGAACAHASRIVAEGTSADRQMAVFAAARRSGARERAALS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHH
AVVDHLAAETAAGSKSGPGGHPNIAPAA
HHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA