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The map label for this gene is gyaR [H]

Identifier: 170749295

GI number: 170749295

Start: 3039692

End: 3040648

Strand: Reverse

Name: gyaR [H]

Synonym: Mrad2831_2888

Alternate gene names: 170749295

Gene position: 3040648-3039692 (Counterclockwise)

Preceding gene: 170749296

Following gene: 170749290

Centisome position: 50.03

GC content: 72.94

Gene sequence:

>957_bases
ATGCGCACCTGCCTGATCCTCGACGATTACCAGCAGGCGGCTCTCGCGCTCGCGGACTGGACGCGCCTCTCCGACCGCGT
CGCCGTCTCCGCGATCGCCCAGCACATCGCGAATCCCGACGATCTCGTGGCGCGGATCGCGCAGGCCGAGATCCTCGTGA
TCATGCGGGAGCGGACGCCGTTCCCGGCGGCGCTGCTGGAGCGGCTGCCCCGGCTCGAGCTGCTCGTGACCAGCGGCATG
CGCAACCTCGCCATCGACCTCGCGGCGGCCAGGGCGCGCAACGTCGTCGTCTGCGGCACGGATTCGAGCCCGACGCCGCC
GACGGAGCTGACCTGGGCGCTGATCCTCGGACTGGCCCGTCACGTGGCGCGGGAGAGCCTGACCCTGCGGGCAGGCGGCC
CCTGGCAGAGCACGATCGGCTCCGACCTCGCGGGTGCGACCCTCGGCGTCCTCGGCCTCGGGAAGATCGGGACTCGGGTG
GCCGAGATCGGGCGCGCCTTCGGCATGCGGGTGATCGCCTGGAGCCCGAATCTCGACGACGCCCGCGCCGCCGCCGCCGG
GGTGGAGCGGGCGGCCTCGAAGGAGGCCCTGCTGGAGGCGAGCGACGTCGTCACGATCCACCTCGTGCTGGGCGACCGGA
CGCGCGGCCTGCTCGGCGCGGCCGAGCTGCGCCGCATGCGCCGCGACGCCTTTCTCGTGAACACCTCGCGCGCGCCGATC
GTCGATCAGGCCGCGCTGCTCCAGGCCCTGGAGGAAGGCTGGATCGCGGGAGCCGGCCTCGACGTGTTCGAGACCGAGCC
CCTTCCGGCCGACAGCCCGTTCCGGCGCCTGCCCAACGTGCTGGCCGTGCCGCATCTCGGCTACGTGTCGCGCAGCAACT
ACCGGACGTTCTTCACCCAGGCGGTGGAGGACATCGAGGCTTGGCTCGCCGGTGGCCCGATCCGGCGCCTGGGCTGA

Upstream 100 bases:

>100_bases
GGCAGCGTTCGCGGCTCTGGCCGGCGCCTAGGAACAGGGCGCCGCGCGTCGTCGCACGGCGACCGTTGACGCACCTTCCA
CCGAGGCCTAACCCCCTCTC

Downstream 100 bases:

>100_bases
GTCCGGGTACGCCGCCCGACGCGGCCTCAGTCCCCGCGGGGATCCAGCGCGTCCGCGAGGCCGTCGCCGATCAGGTTGAG
CGCCACCAGGGTCGCGATCA

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MRTCLILDDYQQAALALADWTRLSDRVAVSAIAQHIANPDDLVARIAQAEILVIMRERTPFPAALLERLPRLELLVTSGM
RNLAIDLAAARARNVVVCGTDSSPTPPTELTWALILGLARHVARESLTLRAGGPWQSTIGSDLAGATLGVLGLGKIGTRV
AEIGRAFGMRVIAWSPNLDDARAAAAGVERAASKEALLEASDVVTIHLVLGDRTRGLLGAAELRRMRRDAFLVNTSRAPI
VDQAALLQALEEGWIAGAGLDVFETEPLPADSPFRRLPNVLAVPHLGYVSRSNYRTFFTQAVEDIEAWLAGGPIRRLG

Sequences:

>Translated_318_residues
MRTCLILDDYQQAALALADWTRLSDRVAVSAIAQHIANPDDLVARIAQAEILVIMRERTPFPAALLERLPRLELLVTSGM
RNLAIDLAAARARNVVVCGTDSSPTPPTELTWALILGLARHVARESLTLRAGGPWQSTIGSDLAGATLGVLGLGKIGTRV
AEIGRAFGMRVIAWSPNLDDARAAAAGVERAASKEALLEASDVVTIHLVLGDRTRGLLGAAELRRMRRDAFLVNTSRAPI
VDQAALLQALEEGWIAGAGLDVFETEPLPADSPFRRLPNVLAVPHLGYVSRSNYRTFFTQAVEDIEAWLAGGPIRRLG
>Mature_318_residues
MRTCLILDDYQQAALALADWTRLSDRVAVSAIAQHIANPDDLVARIAQAEILVIMRERTPFPAALLERLPRLELLVTSGM
RNLAIDLAAARARNVVVCGTDSSPTPPTELTWALILGLARHVARESLTLRAGGPWQSTIGSDLAGATLGVLGLGKIGTRV
AEIGRAFGMRVIAWSPNLDDARAAAAGVERAASKEALLEASDVVTIHLVLGDRTRGLLGAAELRRMRRDAFLVNTSRAPI
VDQAALLQALEEGWIAGAGLDVFETEPLPADSPFRRLPNVLAVPHLGYVSRSNYRTFFTQAVEDIEAWLAGGPIRRLG

Specific function: Unknown

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557497, Length=256, Percent_Identity=34.375, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI61743967, Length=256, Percent_Identity=34.375, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI23308577, Length=255, Percent_Identity=32.9411764705882, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI145580578, Length=237, Percent_Identity=35.8649789029536, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI4557499, Length=237, Percent_Identity=35.8649789029536, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI145580575, Length=235, Percent_Identity=36.1702127659575, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI6912396, Length=245, Percent_Identity=30.6122448979592, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI87082289, Length=282, Percent_Identity=35.8156028368794, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789279, Length=270, Percent_Identity=28.5185185185185, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787645, Length=244, Percent_Identity=30.327868852459, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI87081824, Length=160, Percent_Identity=31.875, Blast_Score=74, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=294, Percent_Identity=32.6530612244898, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25147481, Length=257, Percent_Identity=29.5719844357977, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=299, Percent_Identity=27.0903010033445, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322116, Length=264, Percent_Identity=28.4090909090909, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324964, Length=275, Percent_Identity=29.4545454545455, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=185, Percent_Identity=30.8108108108108, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324980, Length=159, Percent_Identity=30.8176100628931, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19921140, Length=233, Percent_Identity=38.1974248927039, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24646446, Length=257, Percent_Identity=31.9066147859922, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24646448, Length=257, Percent_Identity=31.9066147859922, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24646452, Length=257, Percent_Identity=31.9066147859922, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24646450, Length=257, Percent_Identity=31.9066147859922, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI62472511, Length=257, Percent_Identity=31.9066147859922, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571528, Length=247, Percent_Identity=29.5546558704453, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28574286, Length=257, Percent_Identity=30.3501945525292, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45552429, Length=181, Percent_Identity=30.3867403314917, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45551003, Length=181, Percent_Identity=30.3867403314917, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585514, Length=181, Percent_Identity=30.3867403314917, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28574282, Length=181, Percent_Identity=30.3867403314917, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28574284, Length=181, Percent_Identity=30.3867403314917, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24585516, Length=178, Percent_Identity=28.6516853932584, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 34197; Mature: 34197

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTCLILDDYQQAALALADWTRLSDRVAVSAIAQHIANPDDLVARIAQAEILVIMRERTP
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEECCCC
FPAALLERLPRLELLVTSGMRNLAIDLAAARARNVVVCGTDSSPTPPTELTWALILGLAR
CHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHH
HVARESLTLRAGGPWQSTIGSDLAGATLGVLGLGKIGTRVAEIGRAFGMRVIAWSPNLDD
HHHHHCEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCH
ARAAAAGVERAASKEALLEASDVVTIHLVLGDRTRGLLGAAELRRMRRDAFLVNTSRAPI
HHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHHEEEECCCCCC
VDQAALLQALEEGWIAGAGLDVFETEPLPADSPFRRLPNVLAVPHLGYVSRSNYRTFFTQ
HHHHHHHHHHHHCCEECCCCCCEECCCCCCCCHHHHCCHHHCCCCCCCCCCCCHHHHHHH
AVEDIEAWLAGGPIRRLG
HHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MRTCLILDDYQQAALALADWTRLSDRVAVSAIAQHIANPDDLVARIAQAEILVIMRERTP
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEECCCC
FPAALLERLPRLELLVTSGMRNLAIDLAAARARNVVVCGTDSSPTPPTELTWALILGLAR
CHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHH
HVARESLTLRAGGPWQSTIGSDLAGATLGVLGLGKIGTRVAEIGRAFGMRVIAWSPNLDD
HHHHHCEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCH
ARAAAAGVERAASKEALLEASDVVTIHLVLGDRTRGLLGAAELRRMRRDAFLVNTSRAPI
HHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHHEEEECCCCCC
VDQAALLQALEEGWIAGAGLDVFETEPLPADSPFRRLPNVLAVPHLGYVSRSNYRTFFTQ
HHHHHHHHHHHHCCEECCCCCCEECCCCCCCCHHHHCCHHHCCCCCCCCCCCCHHHHHHH
AVEDIEAWLAGGPIRRLG
HHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]