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The map label for this gene is crp [H]

Identifier: 170749281

GI number: 170749281

Start: 3023723

End: 3024409

Strand: Reverse

Name: crp [H]

Synonym: Mrad2831_2874

Alternate gene names: 170749281

Gene position: 3024409-3023723 (Counterclockwise)

Preceding gene: 170749283

Following gene: 170749280

Centisome position: 49.76

GC content: 70.6

Gene sequence:

>687_bases
ATGGCGCGGTTGGGGACAATACCTTTCTTCAAGGATCCCGGCATCGAGCTCAGCCCCTACGAGCCCCGCTGCCACTGGCG
CCGCTTCGACGAGAACGAGGTCCTCGTCGACTTCGACGACATCTCCACCGACGTGTACTTCATCGCTTCCGGCGAGGTCC
GCATCCTGAACCGGACCCAGTCCGGCAAGGAGGTCATCCTGGGTGAGATGCGCGGCGGGGCCTTCTTCGGCGAGCTCGCC
GCCATCGACGGGATCGGCCGCTCGGCCAACGTCACGGCGCTGACGCGCGGCGAGGTCTGCGTGGTCCCGGCCCCGGTCTT
CCGGCAGATCGTGTTCGCCTCCGAGGCGATCGCCGACCGGCTGTTCCGGCTCCTGGCCAAGCGCGTGCGCGAGCTGAACA
CGCGGCTCATGGAGCACGCCCTGCTCGACCTCAAGCACCGGCTCTACGCCGAGCTGCTGCGCCTGTCCGTGCCCCGAACC
GGCACGGCGGGCGAGCGGGTGGTCTCGCCGCCGCCCTACCACCACGTCCTCGCCGCGCGGATCGGTTGCCGCCGCGAGCA
GGTGACCCGGGAATTCACCGCGATGGCGGCGGAGGGTTTGGTCGACCGCACGCGCGGCGCCCTGGTCCTGCGCCGGCCGG
ACCTCCTGGAAGCGCGCGTGGCCGAGGCGCTGCGCGAGGATTCCTGA

Upstream 100 bases:

>100_bases
CGGGGCGCGGCTTCACTGTGCGGGCTGCTCCCGTCCCCAGATCTGCTGGCCGGCCCCCGTCAGGGCAGGGACGGAGGCCG
GAGTTGAGGGAACACGATCG

Downstream 100 bases:

>100_bases
TCCGTTGTCCGACAGGCCGGCGCCGCGCCTGCGGCTCGAGGAGCTGACCAAGCGCTTCGGCACCCATCGCGCGGTGGACG
CCGTGTCCCTCGACCTCGAG

Product: cyclic nucleotide-binding protein

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MARLGTIPFFKDPGIELSPYEPRCHWRRFDENEVLVDFDDISTDVYFIASGEVRILNRTQSGKEVILGEMRGGAFFGELA
AIDGIGRSANVTALTRGEVCVVPAPVFRQIVFASEAIADRLFRLLAKRVRELNTRLMEHALLDLKHRLYAELLRLSVPRT
GTAGERVVSPPPYHHVLAARIGCRREQVTREFTAMAAEGLVDRTRGALVLRRPDLLEARVAEALREDS

Sequences:

>Translated_228_residues
MARLGTIPFFKDPGIELSPYEPRCHWRRFDENEVLVDFDDISTDVYFIASGEVRILNRTQSGKEVILGEMRGGAFFGELA
AIDGIGRSANVTALTRGEVCVVPAPVFRQIVFASEAIADRLFRLLAKRVRELNTRLMEHALLDLKHRLYAELLRLSVPRT
GTAGERVVSPPPYHHVLAARIGCRREQVTREFTAMAAEGLVDRTRGALVLRRPDLLEARVAEALREDS
>Mature_227_residues
ARLGTIPFFKDPGIELSPYEPRCHWRRFDENEVLVDFDDISTDVYFIASGEVRILNRTQSGKEVILGEMRGGAFFGELAA
IDGIGRSANVTALTRGEVCVVPAPVFRQIVFASEAIADRLFRLLAKRVRELNTRLMEHALLDLKHRLYAELLRLSVPRTG
TAGERVVSPPPYHHVLAARIGCRREQVTREFTAMAAEGLVDRTRGALVLRRPDLLEARVAEALREDS

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=192, Percent_Identity=27.0833333333333, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25595; Mature: 25464

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLGTIPFFKDPGIELSPYEPRCHWRRFDENEVLVDFDDISTDVYFIASGEVRILNRTQ
CCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCCCEEEEECCCEEEEECCC
SGKEVILGEMRGGAFFGELAAIDGIGRSANVTALTRGEVCVVPAPVFRQIVFASEAIADR
CCCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHH
LFRLLAKRVRELNTRLMEHALLDLKHRLYAELLRLSVPRTGTAGERVVSPPPYHHVLAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
IGCRREQVTREFTAMAAEGLVDRTRGALVLRRPDLLEARVAEALREDS
HCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARLGTIPFFKDPGIELSPYEPRCHWRRFDENEVLVDFDDISTDVYFIASGEVRILNRTQ
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCCCEEEEECCCEEEEECCC
SGKEVILGEMRGGAFFGELAAIDGIGRSANVTALTRGEVCVVPAPVFRQIVFASEAIADR
CCCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHH
LFRLLAKRVRELNTRLMEHALLDLKHRLYAELLRLSVPRTGTAGERVVSPPPYHHVLAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
IGCRREQVTREFTAMAAEGLVDRTRGALVLRRPDLLEARVAEALREDS
HCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]