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The map label for this gene is oxlT [H]

Identifier: 170749199

GI number: 170749199

Start: 2931379

End: 2932656

Strand: Reverse

Name: oxlT [H]

Synonym: Mrad2831_2792

Alternate gene names: 170749199

Gene position: 2932656-2931379 (Counterclockwise)

Preceding gene: 170749204

Following gene: 170749198

Centisome position: 48.25

GC content: 67.29

Gene sequence:

>1278_bases
ATGTCCGAGATCGTCAAGCCGGTGGGGCGCGGCCGCTGGCTGCAACTCGCCTTCGGCGTCGTCTGCATGTGCATGATCGC
CAACATGCAGTACGGCTGGACCTTCTTCGTGAGCCCGATGCAGGAGCGCCACGGCTGGGATCGCGCGGCGATCCAGGTGG
CCTTCACGCTGTTCGTCGTCACCGAGACCTGGCTGGTCCCGATCGAGGGCTGGTTCGTCGATAAGTACGGCCCGCGGATC
GTCACGCTGTTCGGCGGTCTGCTCTGCGCCATCGCGTGGGTGATCAACTCCTACGCGGATTCGCTCACCGTCCTCTACAT
CGCGGCCGCGATCGGCGGCACCGGTGCGGGCGCGGTCTACGGAACCTGCGTCGGCAACTCGCTGAAGTGGTTCCCCGATC
GCCGCGGCCTGGCCGCCGGCATCACCGCCATGGGTTTCGGCGCGGGTTCGGCGCTGACGGTCGTGCCGATCCAGGCGATG
ATCAAGTCCCAGGGCTACGAGGCGGCCTTCTTCTACTTCGGCATCGGGCAGGGCGTGATCGTCATGCTGATCGCGCTGTT
CCTGCGCGCGCCGGCCAAGGGTCAGGTTCCGGAGGTCGCCCGGGTCAGCCAGTCGAAGCGGGACTACAAGCCGTCCGAGA
TGGTCCGCACGCCGATCTTCTGGGTCATGTACGCGATGTTCGTCATGATGGCCGCCGGCGGTCTGATGGCCACCGCGCAG
CTCGGCCCGATCGCCAAGGACTTCAAGATCGCCGACGTCCCGGTCTCGCTCCTCGGGATCACGCTGCCGGCGCTGACCTT
CGCGGCCACGCTCGACCGGGTGCTCAACGGCGTGACGCGTCCGTTCTTCGGCTGGGTCTCCGACCATATCGGCCGCGAGA
ACACGATGTTCCTGTCCTTCGCGATCGAGGGCCTGGGCATCTACGCGCTGAGCCAGTTCGGCCAGAACCCGATCGCCTTC
GTGCTGCTGACGGGTCTCGTCTTCTTCGCCTGGGGCGAGATCTACTCGCTGTTCCCGGCGACCTGCGGCGACACGTTCGG
GTCGAAATACGCGGCGACGAATGCCGGCCTGCTCTACACGGCCAAGGGCACCGCGGCGCTGATCGTCCCCTACACCAGCG
TGCTCACGACCATGACCGGGAGCTGGCACACGGTGTTCCTGGCGGCCGCGGCCCTCAACATCCTGGCGGCCCTGCTGGCG
CTGTTCGTCCTCAAGCCGATGCGCGCCGCCTACACCAAGAAGCCCGAGGCGGGCCTCGCGCCGGTGCTGGCGCAGTAG

Upstream 100 bases:

>100_bases
AGAATTCCGGTCAAGTTGTTCTGCCAAGCTGCGCTCTGGCAAGGCATAAAGCTGGCCTGAGCGCGAACGCCAACAATAAT
ACTGTCCAGGAGGGAACGGT

Downstream 100 bases:

>100_bases
CAGGCCCCGGTCGTCCGGCGCCGCGACGCCGAACCGACGTGATGAGCCCGGCGCCGTCTTTACGGCGCCGGGCTTCCTGC
ATACCGGGAACCGGGCCTTC

Product: major facilitator transporter

Products: NA

Alternate protein names: OFA; Oxalate:formate antiport protein; Oxalate:formate exchange protein [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSEIVKPVGRGRWLQLAFGVVCMCMIANMQYGWTFFVSPMQERHGWDRAAIQVAFTLFVVTETWLVPIEGWFVDKYGPRI
VTLFGGLLCAIAWVINSYADSLTVLYIAAAIGGTGAGAVYGTCVGNSLKWFPDRRGLAAGITAMGFGAGSALTVVPIQAM
IKSQGYEAAFFYFGIGQGVIVMLIALFLRAPAKGQVPEVARVSQSKRDYKPSEMVRTPIFWVMYAMFVMMAAGGLMATAQ
LGPIAKDFKIADVPVSLLGITLPALTFAATLDRVLNGVTRPFFGWVSDHIGRENTMFLSFAIEGLGIYALSQFGQNPIAF
VLLTGLVFFAWGEIYSLFPATCGDTFGSKYAATNAGLLYTAKGTAALIVPYTSVLTTMTGSWHTVFLAAAALNILAALLA
LFVLKPMRAAYTKKPEAGLAPVLAQ

Sequences:

>Translated_425_residues
MSEIVKPVGRGRWLQLAFGVVCMCMIANMQYGWTFFVSPMQERHGWDRAAIQVAFTLFVVTETWLVPIEGWFVDKYGPRI
VTLFGGLLCAIAWVINSYADSLTVLYIAAAIGGTGAGAVYGTCVGNSLKWFPDRRGLAAGITAMGFGAGSALTVVPIQAM
IKSQGYEAAFFYFGIGQGVIVMLIALFLRAPAKGQVPEVARVSQSKRDYKPSEMVRTPIFWVMYAMFVMMAAGGLMATAQ
LGPIAKDFKIADVPVSLLGITLPALTFAATLDRVLNGVTRPFFGWVSDHIGRENTMFLSFAIEGLGIYALSQFGQNPIAF
VLLTGLVFFAWGEIYSLFPATCGDTFGSKYAATNAGLLYTAKGTAALIVPYTSVLTTMTGSWHTVFLAAAALNILAALLA
LFVLKPMRAAYTKKPEAGLAPVLAQ
>Mature_424_residues
SEIVKPVGRGRWLQLAFGVVCMCMIANMQYGWTFFVSPMQERHGWDRAAIQVAFTLFVVTETWLVPIEGWFVDKYGPRIV
TLFGGLLCAIAWVINSYADSLTVLYIAAAIGGTGAGAVYGTCVGNSLKWFPDRRGLAAGITAMGFGAGSALTVVPIQAMI
KSQGYEAAFFYFGIGQGVIVMLIALFLRAPAKGQVPEVARVSQSKRDYKPSEMVRTPIFWVMYAMFVMMAAGGLMATAQL
GPIAKDFKIADVPVSLLGITLPALTFAATLDRVLNGVTRPFFGWVSDHIGRENTMFLSFAIEGLGIYALSQFGQNPIAFV
LLTGLVFFAWGEIYSLFPATCGDTFGSKYAATNAGLLYTAKGTAALIVPYTSVLTTMTGSWHTVFLAAAALNILAALLAL
FVLKPMRAAYTKKPEAGLAPVLAQ

Specific function: Anion transporter that carries out the exchange of divalent oxalate with monovalent formate, the product of oxalate decarboxylation, at the plasma membrane, and in doing so catalyzes the vectorial portion of a proton-motive metabolic cycle that drives ATP

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. OFA (TC 2.A.1.11) family [H]

Homologues:

Organism=Escherichia coli, GI1789969, Length=381, Percent_Identity=24.1469816272966, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004741 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45720; Mature: 45589

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIVKPVGRGRWLQLAFGVVCMCMIANMQYGWTFFVSPMQERHGWDRAAIQVAFTLFVV
CCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHH
TETWLVPIEGWFVDKYGPRIVTLFGGLLCAIAWVINSYADSLTVLYIAAAIGGTGAGAVY
HHHEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
GTCVGNSLKWFPDRRGLAAGITAMGFGAGSALTVVPIQAMIKSQGYEAAFFYFGIGQGVI
EEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCEEEEEEECCHHHH
VMLIALFLRAPAKGQVPEVARVSQSKRDYKPSEMVRTPIFWVMYAMFVMMAAGGLMATAQ
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHH
LGPIAKDFKIADVPVSLLGITLPALTFAATLDRVLNGVTRPFFGWVSDHIGRENTMFLSF
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
AIEGLGIYALSQFGQNPIAFVLLTGLVFFAWGEIYSLFPATCGDTFGSKYAATNAGLLYT
EHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEECCCCEEEE
AKGTAALIVPYTSVLTTMTGSWHTVFLAAAALNILAALLALFVLKPMRAAYTKKPEAGLA
ECCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PVLAQ
CCCCC
>Mature Secondary Structure 
SEIVKPVGRGRWLQLAFGVVCMCMIANMQYGWTFFVSPMQERHGWDRAAIQVAFTLFVV
CHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHH
TETWLVPIEGWFVDKYGPRIVTLFGGLLCAIAWVINSYADSLTVLYIAAAIGGTGAGAVY
HHHEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
GTCVGNSLKWFPDRRGLAAGITAMGFGAGSALTVVPIQAMIKSQGYEAAFFYFGIGQGVI
EEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCEEEEEEECCHHHH
VMLIALFLRAPAKGQVPEVARVSQSKRDYKPSEMVRTPIFWVMYAMFVMMAAGGLMATAQ
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHH
LGPIAKDFKIADVPVSLLGITLPALTFAATLDRVLNGVTRPFFGWVSDHIGRENTMFLSF
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
AIEGLGIYALSQFGQNPIAFVLLTGLVFFAWGEIYSLFPATCGDTFGSKYAATNAGLLYT
EHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEECCCCEEEE
AKGTAALIVPYTSVLTTMTGSWHTVFLAAAALNILAALLALFVLKPMRAAYTKKPEAGLA
ECCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PVLAQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8636101; 9651403; 11919184; 11113128; 11274108; 11500368 [H]