Click here to switch to the map view.

The map label for this gene is cspE [H]

Identifier: 170749143

GI number: 170749143

Start: 2876764

End: 2877369

Strand: Reverse

Name: cspE [H]

Synonym: Mrad2831_2736

Alternate gene names: 170749143

Gene position: 2877369-2876764 (Counterclockwise)

Preceding gene: 170749145

Following gene: 170749142

Centisome position: 47.34

GC content: 70.13

Gene sequence:

>606_bases
ATGTCTGACGAACGCGGATCCGGGCCCGAGAACCCCGAGCGGGGGATGATCGCCCGTACCGGCGAGACGGGACAGCACGA
GGATGAGGCGCTCGACCTCGTCGAGGTGGCCGGCCGCATCAAGTGGTTCGACGTTTCGAAGGGCTTCGGGTTCATCGTGC
CCGACGACGGCGCGCCCGACATCCTGCTCCACGTCACCTGCCTGCGGCGCGACGGCTACCAGGCCGCCAGCGAGGGCGCC
CGCATCGTCGTGGAGGCCGTGGAGCGGCCCCGCGGCTGGCAGGCCTTCCGGGTGATCTCCCTCGATCAGGCGACGGCGGT
GCATCCCTCCGAGCTGCCGATGCCGCGCACGCACGTCACCGTGACGCCGACCAGCGGGCTCGAGACCGCCGTCGTGAAGT
GGTTCAACCGCCTCCGCGGGTTCGGATTCCTGACGCGCGGCGACGGGACGCCGGACATCTTCGTGCACATGGAGACGCTG
CGGCGCTACGGTATCGCCGAGCTGAAGCCCGGCGAGTCCGTACTGGTCCGCTACGGCGACGGCTCGAAAGGCGTGATGGC
GGCCGAGGTGCGGCTGATCGACGGAGCCATGCCCTCGTCCCACTGA

Upstream 100 bases:

>100_bases
GCGGCCCTGTTCTGCCGGTCAAATGGGCTTCCCGGACATCAGGACTTCGTTAATCTTCCCGGGAGAGCGCGCGGCGTGAT
TCGGTGTAGGTTGCTCGGCC

Downstream 100 bases:

>100_bases
GGACGCGTTTTCCCGCCAAGCACAGTGCGTCCTATCTCCTTGGTGGGGCTCGGAAAACCCGGGATCGGATTCCCCTCGCG
CGCATCCTGGTCTATCACCG

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: CSP-E [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MSDERGSGPENPERGMIARTGETGQHEDEALDLVEVAGRIKWFDVSKGFGFIVPDDGAPDILLHVTCLRRDGYQAASEGA
RIVVEAVERPRGWQAFRVISLDQATAVHPSELPMPRTHVTVTPTSGLETAVVKWFNRLRGFGFLTRGDGTPDIFVHMETL
RRYGIAELKPGESVLVRYGDGSKGVMAAEVRLIDGAMPSSH

Sequences:

>Translated_201_residues
MSDERGSGPENPERGMIARTGETGQHEDEALDLVEVAGRIKWFDVSKGFGFIVPDDGAPDILLHVTCLRRDGYQAASEGA
RIVVEAVERPRGWQAFRVISLDQATAVHPSELPMPRTHVTVTPTSGLETAVVKWFNRLRGFGFLTRGDGTPDIFVHMETL
RRYGIAELKPGESVLVRYGDGSKGVMAAEVRLIDGAMPSSH
>Mature_200_residues
SDERGSGPENPERGMIARTGETGQHEDEALDLVEVAGRIKWFDVSKGFGFIVPDDGAPDILLHVTCLRRDGYQAASEGAR
IVVEAVERPRGWQAFRVISLDQATAVHPSELPMPRTHVTVTPTSGLETAVVKWFNRLRGFGFLTRGDGTPDIFVHMETLR
RYGIAELKPGESVLVRYGDGSKGVMAAEVRLIDGAMPSSH

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1786841, Length=69, Percent_Identity=37.6811594202899, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=38.8059701492537, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 21984; Mature: 21853

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDERGSGPENPERGMIARTGETGQHEDEALDLVEVAGRIKWFDVSKGFGFIVPDDGAPD
CCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCCC
ILLHVTCLRRDGYQAASEGARIVVEAVERPRGWQAFRVISLDQATAVHPSELPMPRTHVT
EEEEEEEECCCCCCCHHCCCEEEEEEHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEE
VTPTSGLETAVVKWFNRLRGFGFLTRGDGTPDIFVHMETLRRYGIAELKPGESVLVRYGD
ECCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEEHHHHHCCCCEECCCCEEEEEECC
GSKGVMAAEVRLIDGAMPSSH
CCCCEEEEEEEEEECCCCCCC
>Mature Secondary Structure 
SDERGSGPENPERGMIARTGETGQHEDEALDLVEVAGRIKWFDVSKGFGFIVPDDGAPD
CCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCCC
ILLHVTCLRRDGYQAASEGARIVVEAVERPRGWQAFRVISLDQATAVHPSELPMPRTHVT
EEEEEEEECCCCCCCHHCCCEEEEEEHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEE
VTPTSGLETAVVKWFNRLRGFGFLTRGDGTPDIFVHMETLRRYGIAELKPGESVLVRYGD
ECCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEEHHHHHCCCCEECCCCEEEEEECC
GSKGVMAAEVRLIDGAMPSSH
CCCCEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]