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The map label for this gene is ysgA [C]

Identifier: 170749140

GI number: 170749140

Start: 2874640

End: 2875512

Strand: Reverse

Name: ysgA [C]

Synonym: Mrad2831_2733

Alternate gene names: 170749140

Gene position: 2875512-2874640 (Counterclockwise)

Preceding gene: 170749141

Following gene: 170749139

Centisome position: 47.31

GC content: 72.05

Gene sequence:

>873_bases
ATGGCGGATCACGACCGGGCCGGACTCGACGGGCTGATCACGCCGCCCCTGTCGCGGCGGGGCTTCGTGATGACGAGCCT
GATGAGCGGCCTGACGCTCGCGACCACCCGCGTCGAGGCCCAGGTGATCCACACGGACAGTGCGGGCATCGAGGCCGGCG
AGGTGAAGATCCCGGCTGCGGACGGCCCGATGCCGGCCTACCGGGCGGTTCCGGAGGGCGCGGGCCCGTTCCCGATCGTC
CTGGTAATCGAGGAGATCTTCGGCGTCCACGACTACATCAAGGACATCTGTCGCCGGCTCGCGAAGGCCGGTTACTGCGC
CGTGGCCCCCGAGCTCTACGCCCGCCAGGGCGACCTCTCGACCATGTCGGATCCCAAGCAGATCATCCGGGACGTCATCG
CTAAGACACCGGACGCGCAGTGGATCGGCGATCTCGACGCGACGGTGGCCTGGGCGGCCTCCGCGGCGAAGGGCGACCCG
AACCGTCTGGGCGTCATGGGCTGGTGCCGCGGCGGGCGCGACGCGTGGCTCTACGCGGCCCATCGTCGCGACCTGAAGGC
GGCCGTGGCCTGGTACGGACCGATCGGCGGCGAGCGGACCGACATCCAGCCGCGCACCGCCGGAGACGTGGCGGCCGAGA
TCCACGCCCCGCTCCTCGCCCTCTACGGCGGGGCCGATGCCGGGATCCCCGTGGCGGCGGTCGAGGAGGCGCGCGACCGC
GCCAAGGCGGCGGGCAAGAGTGTCGAGCTCGTGATCTACCCGGAGGCGCCGCACGGCTTCCACGCGGATTACCGTCCGAG
CTACCGGCGCGAGGCCGCGGAGGATGGCTGGGCGCGCGCGCTGGCCTTCCTGAAATCGCACGGCGTCGGCTGA

Upstream 100 bases:

>100_bases
TGGCGGCTGCCGGGGCCTGACGGCCCGGTAACGGCGGGCGATTTGACCCGGGCCGCCGCGCGCGGGTAGCCCTCATCCTC
GAACCGAGGGAGAGAGCACC

Downstream 100 bases:

>100_bases
ATAGACGCCTCAGGACGCCCGATCGAGCGCGTTCCGGGACGGCTGTGTTACCCAAGACACGGCCACAACTTCACAATCGT
GCAAGGATCCAAGGAGGATC

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MADHDRAGLDGLITPPLSRRGFVMTSLMSGLTLATTRVEAQVIHTDSAGIEAGEVKIPAADGPMPAYRAVPEGAGPFPIV
LVIEEIFGVHDYIKDICRRLAKAGYCAVAPELYARQGDLSTMSDPKQIIRDVIAKTPDAQWIGDLDATVAWAASAAKGDP
NRLGVMGWCRGGRDAWLYAAHRRDLKAAVAWYGPIGGERTDIQPRTAGDVAAEIHAPLLALYGGADAGIPVAAVEEARDR
AKAAGKSVELVIYPEAPHGFHADYRPSYRREAAEDGWARALAFLKSHGVG

Sequences:

>Translated_290_residues
MADHDRAGLDGLITPPLSRRGFVMTSLMSGLTLATTRVEAQVIHTDSAGIEAGEVKIPAADGPMPAYRAVPEGAGPFPIV
LVIEEIFGVHDYIKDICRRLAKAGYCAVAPELYARQGDLSTMSDPKQIIRDVIAKTPDAQWIGDLDATVAWAASAAKGDP
NRLGVMGWCRGGRDAWLYAAHRRDLKAAVAWYGPIGGERTDIQPRTAGDVAAEIHAPLLALYGGADAGIPVAAVEEARDR
AKAAGKSVELVIYPEAPHGFHADYRPSYRREAAEDGWARALAFLKSHGVG
>Mature_289_residues
ADHDRAGLDGLITPPLSRRGFVMTSLMSGLTLATTRVEAQVIHTDSAGIEAGEVKIPAADGPMPAYRAVPEGAGPFPIVL
VIEEIFGVHDYIKDICRRLAKAGYCAVAPELYARQGDLSTMSDPKQIIRDVIAKTPDAQWIGDLDATVAWAASAAKGDPN
RLGVMGWCRGGRDAWLYAAHRRDLKAAVAWYGPIGGERTDIQPRTAGDVAAEIHAPLLALYGGADAGIPVAAVEEARDRA
KAAGKSVELVIYPEAPHGFHADYRPSYRREAAEDGWARALAFLKSHGVG

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=265, Percent_Identity=46.4150943396226, Blast_Score=251, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 30915; Mature: 30784

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADHDRAGLDGLITPPLSRRGFVMTSLMSGLTLATTRVEAQVIHTDSAGIEAGEVKIPAA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCCCEEECCC
DGPMPAYRAVPEGAGPFPIVLVIEEIFGVHDYIKDICRRLAKAGYCAVAPELYARQGDLS
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHCCCCCC
TMSDPKQIIRDVIAKTPDAQWIGDLDATVAWAASAAKGDPNRLGVMGWCRGGRDAWLYAA
CCCCHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEHH
HRRDLKAAVAWYGPIGGERTDIQPRTAGDVAAEIHAPLLALYGGADAGIPVAAVEEARDR
HHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHH
AKAAGKSVELVIYPEAPHGFHADYRPSYRREAAEDGWARALAFLKSHGVG
HHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADHDRAGLDGLITPPLSRRGFVMTSLMSGLTLATTRVEAQVIHTDSAGIEAGEVKIPAA
CCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCCCEEECCC
DGPMPAYRAVPEGAGPFPIVLVIEEIFGVHDYIKDICRRLAKAGYCAVAPELYARQGDLS
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHCCCCCC
TMSDPKQIIRDVIAKTPDAQWIGDLDATVAWAASAAKGDPNRLGVMGWCRGGRDAWLYAA
CCCCHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEHH
HRRDLKAAVAWYGPIGGERTDIQPRTAGDVAAEIHAPLLALYGGADAGIPVAAVEEARDR
HHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHH
AKAAGKSVELVIYPEAPHGFHADYRPSYRREAAEDGWARALAFLKSHGVG
HHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9168622 [H]