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The map label for this gene is fabI2 [H]

Identifier: 170749089

GI number: 170749089

Start: 2826005

End: 2826835

Strand: Reverse

Name: fabI2 [H]

Synonym: Mrad2831_2682

Alternate gene names: 170749089

Gene position: 2826835-2826005 (Counterclockwise)

Preceding gene: 170749090

Following gene: 170749087

Centisome position: 46.51

GC content: 70.04

Gene sequence:

>831_bases
ATGACGGGTTTGATGGCAGGCAAGCGCGGCCTGATCATGGGCGTCGCGAACGATCACTCGATCGCCTGGGGGATCGCCAA
GGCGCTGCACGCCCAGGGCGCGCGACTGGCTTTCACCTATCAGGGCGAGGCGCTGGGCCGACGGGTGACGCCGCTCGCCG
CCAAGCTCGATTCGGACATCGTGGTGCCCTGCGACGTCGAGGATCTCGCCTCCGTCGACGCGACCTTCGAGGCCCTCGAC
GCGCGGTTCGACGGCGGTCTCGACTTCGTGGTCCACGCCATCGGTTTCTCCGACAAGGCGCAGCTCAAGGGGCGCTACGT
CGACGTGACGACGCGCGAGAACTTCGTGCGCACGATGACGATCTCGTGCTTCTCGTTCACCGAGATCGCGCAGCGCGCGG
CCAAGCGGATGAACCAGGGCGGGTCGCTGCTGACCCTGACCTACGGCGGCGCCACCCGGGTCATGCCGAACTACAACGTC
ATGGGCGTCGCCAAGGCGGCGCTCGAGGCCTCGGTGCGCTACCTGGCGAGCGACCTGGGCCCGGACGGGATCCGGGTCAA
CGCGCTTTCCGCCGGCCCGATGCGGACCCTGGCGGGCGCCGGGATCGCCGACGCGCGGCTGATGTTCAATCACCAGCGGG
CGCACGCGCCGCTGCGGCGCACGGTGAGCCTGGAGGATGTCGGCGGGTCGGCCCTCTACCTGCTGTCGCCGCTCTCGGGC
GGCGTCACCGGCGAGGTCCACTTCGTGGATGCCGGCTACAACATCATCTCGATGCCGCGCCCGGACGTGCTGCAGGCGCA
GGACGCGGCCGGCGTCGTCGGCGACGCCTGA

Upstream 100 bases:

>100_bases
CGCGAGCCGCGTTAAGGCAGCCTGCCGCGGTTCGCTCGTCGCGACCGCGGTCCGGGCCCGCTCCGCGGGTCCGGCGTGCT
CGGTCAGGATGTGAGTCGGC

Downstream 100 bases:

>100_bases
GGCGCCTCACTCCTCCGGAATGCGCGCCGGGGGCAGGGGACCGGCCGGGACGCCGCGCTCGCCGCGGACTGCCCGCCGCT
CCCGCGCGGCGCGCGCCTCC

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MTGLMAGKRGLIMGVANDHSIAWGIAKALHAQGARLAFTYQGEALGRRVTPLAAKLDSDIVVPCDVEDLASVDATFEALD
ARFDGGLDFVVHAIGFSDKAQLKGRYVDVTTRENFVRTMTISCFSFTEIAQRAAKRMNQGGSLLTLTYGGATRVMPNYNV
MGVAKAALEASVRYLASDLGPDGIRVNALSAGPMRTLAGAGIADARLMFNHQRAHAPLRRTVSLEDVGGSALYLLSPLSG
GVTGEVHFVDAGYNIISMPRPDVLQAQDAAGVVGDA

Sequences:

>Translated_276_residues
MTGLMAGKRGLIMGVANDHSIAWGIAKALHAQGARLAFTYQGEALGRRVTPLAAKLDSDIVVPCDVEDLASVDATFEALD
ARFDGGLDFVVHAIGFSDKAQLKGRYVDVTTRENFVRTMTISCFSFTEIAQRAAKRMNQGGSLLTLTYGGATRVMPNYNV
MGVAKAALEASVRYLASDLGPDGIRVNALSAGPMRTLAGAGIADARLMFNHQRAHAPLRRTVSLEDVGGSALYLLSPLSG
GVTGEVHFVDAGYNIISMPRPDVLQAQDAAGVVGDA
>Mature_275_residues
TGLMAGKRGLIMGVANDHSIAWGIAKALHAQGARLAFTYQGEALGRRVTPLAAKLDSDIVVPCDVEDLASVDATFEALDA
RFDGGLDFVVHAIGFSDKAQLKGRYVDVTTRENFVRTMTISCFSFTEIAQRAAKRMNQGGSLLTLTYGGATRVMPNYNVM
GVAKAALEASVRYLASDLGPDGIRVNALSAGPMRTLAGAGIADARLMFNHQRAHAPLRRTVSLEDVGGSALYLLSPLSGG
VTGEVHFVDAGYNIISMPRPDVLQAQDAAGVVGDA

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=265, Percent_Identity=27.9245283018868, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=52.8957528957529, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI2367365, Length=256, Percent_Identity=27.734375, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787905, Length=141, Percent_Identity=32.6241134751773, Blast_Score=63, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644337, Length=208, Percent_Identity=28.8461538461538, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29113; Mature: 28981

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLMAGKRGLIMGVANDHSIAWGIAKALHAQGARLAFTYQGEALGRRVTPLAAKLDSDI
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHCCCCCHHHHHCCCCE
VVPCDVEDLASVDATFEALDARFDGGLDFVVHAIGFSDKAQLKGRYVDVTTRENFVRTMT
EEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCEEEEEECCCCEEEEEE
ISCFSFTEIAQRAAKRMNQGGSLLTLTYGGATRVMPNYNVMGVAKAALEASVRYLASDLG
EEHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
PDGIRVNALSAGPMRTLAGAGIADARLMFNHQRAHAPLRRTVSLEDVGGSALYLLSPLSG
CCCEEEEEECCCCHHHHHCCCCCHHHHEECCHHHCCCHHHEEEEECCCCCEEEEEECCCC
GVTGEVHFVDAGYNIISMPRPDVLQAQDAAGVVGDA
CCEEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGLMAGKRGLIMGVANDHSIAWGIAKALHAQGARLAFTYQGEALGRRVTPLAAKLDSDI
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHCCCCCHHHHHCCCCE
VVPCDVEDLASVDATFEALDARFDGGLDFVVHAIGFSDKAQLKGRYVDVTTRENFVRTMT
EEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCEEEEEECCCCEEEEEE
ISCFSFTEIAQRAAKRMNQGGSLLTLTYGGATRVMPNYNVMGVAKAALEASVRYLASDLG
EEHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
PDGIRVNALSAGPMRTLAGAGIADARLMFNHQRAHAPLRRTVSLEDVGGSALYLLSPLSG
CCCEEEEEECCCCHHHHHCCCCCHHHHEECCHHHCCCHHHEEEEECCCCCEEEEEECCCC
GVTGEVHFVDAGYNIISMPRPDVLQAQDAAGVVGDA
CCEEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]