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The map label for this gene is leuA [H]

Identifier: 170749073

GI number: 170749073

Start: 2808677

End: 2810242

Strand: Reverse

Name: leuA [H]

Synonym: Mrad2831_2666

Alternate gene names: 170749073

Gene position: 2810242-2808677 (Counterclockwise)

Preceding gene: 170749074

Following gene: 170749070

Centisome position: 46.24

GC content: 69.67

Gene sequence:

>1566_bases
ATGACCACGACCAGCACCGCCTCCGCCCGCGACCGGATCCTGATCTTCGACACCACGCTGCGCGACGGCGAGCAGTGCCC
CGGCGCCACCATGACCTTCGACGAGAAGCTCGCGGTGGCCGAGTTGCTCGACACGATGGGCGTCGACATCATCGAGGCCG
GGTTCCCCATCGCGTCGAACGGCGATTTCGAGGCGGTCTCCGAGATCGCCCGCCGGACCAAGCGCGCGACCATCGCGGGC
CTCGCCCGCGCCATCCCCGCCGACATCGCCCGGGCCGGCGAGGCCGTGCGCCACGCCAAGCGCGCGCGGATCCACACCTT
CGTGTCGACCTCGCCGATCCATCTCGCGCACCAGATGCGCAAGAGCCAGGACGAGGTCGTCGAGATCATCCTGAAGACCG
TGGCCCAGGCCCGCGACCTCGTCGAGGACGTGGAGTGGTCGGCGATGGACGCGACCCGCACGCCGCTCGACTACCTGTGC
CGCTGCGTCGAGGCGGCGATCAAGGCCGGCGCCACGACGATCAACCTCCCCGATACGGTCGGCTACGCCACGCCGTCGGA
GTACCGCGACATGTTCGCGAACGTCCGCGCGCGCGTGCCGAACGCCGACAAGGCGGTGTTCTCGGTCCATTGCCACAACG
ACCTGGGCCTCGCGATCGCCAATTCCCTGGCGGGCATCGAGGGCGGCGCCCGGCAGGTCGAGTGCACGATCAACGGCATC
GGCGAGCGGGCCGGCAACGCCGCGCTCGAGGAGATCGTGATGGCGCTGCGCACCCGCGCGGACGTGCTGCCCTACGAGAC
CGGCGTCGAATCGACCATGCTGACCCGCGCCTCGAAGCTCGTGAGCCACGCCACGAACTTCCCGGTGCAGTTCAACAAGG
CGATCGTCGGCCGCAACGCCTTCGCGCACGAGAGCGGCATCCACCAGGACGGCATGCTCAAGAACGCCGAGACCTACGAG
ATCATGACGCCCGCCTCGGTCGGGCTGACCAAGACCTCCCTGGTGATGGGCAAGCATTCCGGCCGGGCGGCCTTCCGCTC
CAAGCTGGAGGATCTCGGCCTGCACCTGTCCGACAACCAGTTCCAGGACGCCTTCGACCGCTTCAAGGCCTTGGCCGACC
GGAAGAAGCACGTCTACGACGAGGACATCGAGGCGCTGGTCGACGAGAACCTCGCCACGGCCCACGACCGGATCCGGCTG
GTCTCCCTGTCGGTGATCGCCGGCACCCGCGGCCCGCAGCGCGCGACTTTGCGGATCGCCGACGAGACCGGCACGCGGAT
CGAGGAGGCGGACGGCAACGGGCCGGTGGACGCGGTGTTCAACGCGATCTCGGCGCTGGTCCCGCACCAGGCGCAGCTGG
AGCTCTACCAGGTCCATGCCGTGACCGGCGGCACGGACGCGCAGGCGGAGGTCTCGGTGCGCCTGCGCGACGGCGAGCGC
AGCGTCACCGCGCGCGGCGCCGATCCGGACACGCTGGTGGCGTCCGCGAAGGCGTATCTCTCGGCGCTGAACAAGCTGTC
GGCCCATTCCGTGCGCGTGCATGCACAGCATGCGGCGGCGGAATGA

Upstream 100 bases:

>100_bases
TGGGCCTTCAGGGGAGTTCTTTCATTCGCGAATCCGCCCGCGAGGGCACCACGCCGCGCCGCGCCATCCCGTGCCGGGCG
GCCCGCGACAGGGTAATGCC

Downstream 100 bases:

>100_bases
GAAAAAGCTGCGGCCGGGGCTGGACAGGCCCCGGCCGCGTTGGTATCAGCCCGCCACCCCGGACGGACTTGGTGGCCACC
GGGCGGTCGCAGACGCGATC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MTTTSTASARDRILIFDTTLRDGEQCPGATMTFDEKLAVAELLDTMGVDIIEAGFPIASNGDFEAVSEIARRTKRATIAG
LARAIPADIARAGEAVRHAKRARIHTFVSTSPIHLAHQMRKSQDEVVEIILKTVAQARDLVEDVEWSAMDATRTPLDYLC
RCVEAAIKAGATTINLPDTVGYATPSEYRDMFANVRARVPNADKAVFSVHCHNDLGLAIANSLAGIEGGARQVECTINGI
GERAGNAALEEIVMALRTRADVLPYETGVESTMLTRASKLVSHATNFPVQFNKAIVGRNAFAHESGIHQDGMLKNAETYE
IMTPASVGLTKTSLVMGKHSGRAAFRSKLEDLGLHLSDNQFQDAFDRFKALADRKKHVYDEDIEALVDENLATAHDRIRL
VSLSVIAGTRGPQRATLRIADETGTRIEEADGNGPVDAVFNAISALVPHQAQLELYQVHAVTGGTDAQAEVSVRLRDGER
SVTARGADPDTLVASAKAYLSALNKLSAHSVRVHAQHAAAE

Sequences:

>Translated_521_residues
MTTTSTASARDRILIFDTTLRDGEQCPGATMTFDEKLAVAELLDTMGVDIIEAGFPIASNGDFEAVSEIARRTKRATIAG
LARAIPADIARAGEAVRHAKRARIHTFVSTSPIHLAHQMRKSQDEVVEIILKTVAQARDLVEDVEWSAMDATRTPLDYLC
RCVEAAIKAGATTINLPDTVGYATPSEYRDMFANVRARVPNADKAVFSVHCHNDLGLAIANSLAGIEGGARQVECTINGI
GERAGNAALEEIVMALRTRADVLPYETGVESTMLTRASKLVSHATNFPVQFNKAIVGRNAFAHESGIHQDGMLKNAETYE
IMTPASVGLTKTSLVMGKHSGRAAFRSKLEDLGLHLSDNQFQDAFDRFKALADRKKHVYDEDIEALVDENLATAHDRIRL
VSLSVIAGTRGPQRATLRIADETGTRIEEADGNGPVDAVFNAISALVPHQAQLELYQVHAVTGGTDAQAEVSVRLRDGER
SVTARGADPDTLVASAKAYLSALNKLSAHSVRVHAQHAAAE
>Mature_520_residues
TTTSTASARDRILIFDTTLRDGEQCPGATMTFDEKLAVAELLDTMGVDIIEAGFPIASNGDFEAVSEIARRTKRATIAGL
ARAIPADIARAGEAVRHAKRARIHTFVSTSPIHLAHQMRKSQDEVVEIILKTVAQARDLVEDVEWSAMDATRTPLDYLCR
CVEAAIKAGATTINLPDTVGYATPSEYRDMFANVRARVPNADKAVFSVHCHNDLGLAIANSLAGIEGGARQVECTINGIG
ERAGNAALEEIVMALRTRADVLPYETGVESTMLTRASKLVSHATNFPVQFNKAIVGRNAFAHESGIHQDGMLKNAETYEI
MTPASVGLTKTSLVMGKHSGRAAFRSKLEDLGLHLSDNQFQDAFDRFKALADRKKHVYDEDIEALVDENLATAHDRIRLV
SLSVIAGTRGPQRATLRIADETGTRIEEADGNGPVDAVFNAISALVPHQAQLELYQVHAVTGGTDAQAEVSVRLRDGERS
VTARGADPDTLVASAKAYLSALNKLSAHSVRVHAQHAAAE

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=496, Percent_Identity=45.9677419354839, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320071, Length=378, Percent_Identity=27.5132275132275, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6320019, Length=376, Percent_Identity=27.3936170212766, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324682, Length=536, Percent_Identity=26.3059701492537, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324225, Length=536, Percent_Identity=26.1194029850746, Blast_Score=124, Evalue=5e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56133; Mature: 56002

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTSTASARDRILIFDTTLRDGEQCPGATMTFDEKLAVAELLDTMGVDIIEAGFPIASN
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCC
GDFEAVSEIARRTKRATIAGLARAIPADIARAGEAVRHAKRARIHTFVSTSPIHLAHQMR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHH
KSQDEVVEIILKTVAQARDLVEDVEWSAMDATRTPLDYLCRCVEAAIKAGATTINLPDTV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCC
GYATPSEYRDMFANVRARVPNADKAVFSVHCHNDLGLAIANSLAGIEGGARQVECTINGI
CCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECC
GERAGNAALEEIVMALRTRADVLPYETGVESTMLTRASKLVSHATNFPVQFNKAIVGRNA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECHHHHCCCC
FAHESGIHQDGMLKNAETYEIMTPASVGLTKTSLVMGKHSGRAAFRSKLEDLGLHLSDNQ
HHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHHEEECCCCCHHHHHHHHHHCCCCCCCCH
FQDAFDRFKALADRKKHVYDEDIEALVDENLATAHDRIRLVSLSVIAGTRGPQRATLRIA
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHEEEEEEEEEECCCCCCCEEEEEE
DETGTRIEEADGNGPVDAVFNAISALVPHQAQLELYQVHAVTGGTDAQAEVSVRLRDGER
CCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHEEEEEEEEECCCCCCCEEEEEEEECCCC
SVTARGADPDTLVASAKAYLSALNKLSAHSVRVHAQHAAAE
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCC
>Mature Secondary Structure 
TTTSTASARDRILIFDTTLRDGEQCPGATMTFDEKLAVAELLDTMGVDIIEAGFPIASN
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCC
GDFEAVSEIARRTKRATIAGLARAIPADIARAGEAVRHAKRARIHTFVSTSPIHLAHQMR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHH
KSQDEVVEIILKTVAQARDLVEDVEWSAMDATRTPLDYLCRCVEAAIKAGATTINLPDTV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCC
GYATPSEYRDMFANVRARVPNADKAVFSVHCHNDLGLAIANSLAGIEGGARQVECTINGI
CCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECC
GERAGNAALEEIVMALRTRADVLPYETGVESTMLTRASKLVSHATNFPVQFNKAIVGRNA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECHHHHCCCC
FAHESGIHQDGMLKNAETYEIMTPASVGLTKTSLVMGKHSGRAAFRSKLEDLGLHLSDNQ
HHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHHEEECCCCCHHHHHHHHHHCCCCCCCCH
FQDAFDRFKALADRKKHVYDEDIEALVDENLATAHDRIRLVSLSVIAGTRGPQRATLRIA
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHEEEEEEEEEECCCCCCCEEEEEE
DETGTRIEEADGNGPVDAVFNAISALVPHQAQLELYQVHAVTGGTDAQAEVSVRLRDGER
CCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHEEEEEEEEECCCCCCCEEEEEEEECCCC
SVTARGADPDTLVASAKAYLSALNKLSAHSVRVHAQHAAAE
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA