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The map label for this gene is narY [H]

Identifier: 170749001

GI number: 170749001

Start: 2745172

End: 2746701

Strand: Reverse

Name: narY [H]

Synonym: Mrad2831_2591

Alternate gene names: 170749001

Gene position: 2746701-2745172 (Counterclockwise)

Preceding gene: 170749002

Following gene: 170749000

Centisome position: 45.19

GC content: 65.16

Gene sequence:

>1530_bases
ATGAAAATCCGCGCGCAGATCGCGATGGTTCTCAACCTCGACAAGTGCATCGGCTGCCATACCTGCTCGGTCACCTGTAA
GAACGTCTGGACCAACCGCGAGGGCGTCGAGTACGCTTGGTTCAACAACGTCGAGACCAAGCCCGGCATCGGCTATCCCA
AGGACTGGGAGAACCAGGACAAGTGGAAGGGCGGCTGGACGCGCAGGACGAACGGCAAGATCGAGCCGCGCATCGGCTCG
AAATGGCGCATTCTCGCGAACATCTTCGCCAACCCTGATCTGCCCGAGATCGACGACTACTACGAGCCGTTCGACTTCGA
CTACCAGCATCTGCAACGGGCCCCCGAGGTCCAGGCCTTCCCGACTGCCCGCCCGCGCTCGAAGATCACCGGCGAGCGCA
TGGAGAAGATCGAGTGGGGGCCGAACTGGGAGGAGATCCTGGGTGGCGAGTTCGCCAAGCGCCGGCAGGACAAGAACTTC
GAGGGGGTCGAGGAGGAGATCTACGGCCAGTTCGAGAACACCTTCATGATGTACCTGCCGCGGTTGTGCGAGCACTGCCT
CAACCCGGCCTGCGTCGCCTCCTGCCCGTCGGGCGCGATCTACAAGCGCGAGGAGGACGGCGTCGTCCTGATCGACCAGG
ACAAGTGCCGCGGCTGGCGCATGTGCGTCTCCGGCTGCCCGTACAAGAAGATTTACTACAACTGGTCCTCGGGTAAGTCC
GAGAAGTGCACGCTGTGCTACCCCCGCCTGGAGGCGGGTCAGCCAACCGTGTGCTCGGAGACCTGCGTCGGCCGCATCCG
CTATCTTGGCGTCGTGCTCTACGACGCCGACCGGATCGAGGCGGCCGCCTCGACGCCCGACGAGCAGGACCTGTACGAGG
AACAGCTCAAGGTCTTCCTCGACCCCTCGGATCCGAAGGTCATCTCCGAGGCACGCAAGGCGGGCATCGCCGAGGCTTGG
CTGGAAGCCGCCCGCCGCTCTCCGGTCTACAAGATGGCTATGGAGTGGAAGATCGCCTTCCCGCTCCACCCCGAATACCG
GACCCTGCCGATGGTCTGGTACGTGCCGCCGCTCTCACCCATCCAGTCGGCGGCGTCGGCCGGCAAGATCGGCCTCGACG
GCGAGATGCCGGACGTGAAGAGCCTGCGCATCCCGGTAAAGTACCTCGCCAACCTCCTCACCGCCGGCAAGGAGGAGCCG
GTGGTGACGGGGCTGGAGCGGATGCTGGCCATGCGCGCCTACATGCGCGGCAAAACCTTCGACGGCGTCCTCGACGAGGC
CATCGCGGCCCGGGTCGGGCTGAGCGGCGCGCAGATCGAGGAGATGTACCGGGTCATGGCCATCGCGAACTACGAGGACC
GGTTCGTCATACCGACCAGCCACCGCGAGATGGGCATCGAGGACGCCTACGACCTGCGCGGCTCCTGCGGCTTCTCCTTC
GGGAATGGCTGCTCGGGCGGCACGACGGGCACGAACCTGTTCGGCGCGCCCAAGCCCGCCAAGGCCAAGACACCGATGGA
AGTCGCCTGA

Upstream 100 bases:

>100_bases
CACCCCTTAGGGGACCATCCCGAATGTCCCGCGGCCGGTCCCGCGGGTATCCGTCCAAACCACTGGCGCCGCGGTCAACG
GCAGCCAAGAGGACCCTGCC

Downstream 100 bases:

>100_bases
GGCGTGCCGAGGAGAACTCTCATGAAGACGCTGAAGGCCCTCTCGGCCCTGCTCACCTATCCCTCACGCGACCTCGCTCT
CGCTGTTCCCGAGATCCACG

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTNREGVEYAWFNNVETKPGIGYPKDWENQDKWKGGWTRRTNGKIEPRIGS
KWRILANIFANPDLPEIDDYYEPFDFDYQHLQRAPEVQAFPTARPRSKITGERMEKIEWGPNWEEILGGEFAKRRQDKNF
EGVEEEIYGQFENTFMMYLPRLCEHCLNPACVASCPSGAIYKREEDGVVLIDQDKCRGWRMCVSGCPYKKIYYNWSSGKS
EKCTLCYPRLEAGQPTVCSETCVGRIRYLGVVLYDADRIEAAASTPDEQDLYEEQLKVFLDPSDPKVISEARKAGIAEAW
LEAARRSPVYKMAMEWKIAFPLHPEYRTLPMVWYVPPLSPIQSAASAGKIGLDGEMPDVKSLRIPVKYLANLLTAGKEEP
VVTGLERMLAMRAYMRGKTFDGVLDEAIAARVGLSGAQIEEMYRVMAIANYEDRFVIPTSHREMGIEDAYDLRGSCGFSF
GNGCSGGTTGTNLFGAPKPAKAKTPMEVA

Sequences:

>Translated_509_residues
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTNREGVEYAWFNNVETKPGIGYPKDWENQDKWKGGWTRRTNGKIEPRIGS
KWRILANIFANPDLPEIDDYYEPFDFDYQHLQRAPEVQAFPTARPRSKITGERMEKIEWGPNWEEILGGEFAKRRQDKNF
EGVEEEIYGQFENTFMMYLPRLCEHCLNPACVASCPSGAIYKREEDGVVLIDQDKCRGWRMCVSGCPYKKIYYNWSSGKS
EKCTLCYPRLEAGQPTVCSETCVGRIRYLGVVLYDADRIEAAASTPDEQDLYEEQLKVFLDPSDPKVISEARKAGIAEAW
LEAARRSPVYKMAMEWKIAFPLHPEYRTLPMVWYVPPLSPIQSAASAGKIGLDGEMPDVKSLRIPVKYLANLLTAGKEEP
VVTGLERMLAMRAYMRGKTFDGVLDEAIAARVGLSGAQIEEMYRVMAIANYEDRFVIPTSHREMGIEDAYDLRGSCGFSF
GNGCSGGTTGTNLFGAPKPAKAKTPMEVA
>Mature_509_residues
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTNREGVEYAWFNNVETKPGIGYPKDWENQDKWKGGWTRRTNGKIEPRIGS
KWRILANIFANPDLPEIDDYYEPFDFDYQHLQRAPEVQAFPTARPRSKITGERMEKIEWGPNWEEILGGEFAKRRQDKNF
EGVEEEIYGQFENTFMMYLPRLCEHCLNPACVASCPSGAIYKREEDGVVLIDQDKCRGWRMCVSGCPYKKIYYNWSSGKS
EKCTLCYPRLEAGQPTVCSETCVGRIRYLGVVLYDADRIEAAASTPDEQDLYEEQLKVFLDPSDPKVISEARKAGIAEAW
LEAARRSPVYKMAMEWKIAFPLHPEYRTLPMVWYVPPLSPIQSAASAGKIGLDGEMPDVKSLRIPVKYLANLLTAGKEEP
VVTGLERMLAMRAYMRGKTFDGVLDEAIAARVGLSGAQIEEMYRVMAIANYEDRFVIPTSHREMGIEDAYDLRGSCGFSF
GNGCSGGTTGTNLFGAPKPAKAKTPMEVA

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=499, Percent_Identity=71.3426853707415, Blast_Score=774, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=497, Percent_Identity=72.2334004024145, Blast_Score=773, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=127, Percent_Identity=40.9448818897638, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=43.5643564356436, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=39.3939393939394, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI2367345, Length=101, Percent_Identity=29.7029702970297, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57484; Mature: 57484

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTNREGVEYAWFNNVETKPGIGYPKDWENQD
CCCCEEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWKGGWTRRTNGKIEPRIGSKWRILANIFANPDLPEIDDYYEPFDFDYQHLQRAPEVQAF
CCCCCCEECCCCEECCCCCCCEEEEEHHHCCCCCCCCHHCCCCCCCCHHHHHCCCCCCCC
PTARPRSKITGERMEKIEWGPNWEEILGGEFAKRRQDKNFEGVEEEIYGQFENTFMMYLP
CCCCCCHHCCHHHHHHCCCCCCHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGAIYKREEDGVVLIDQDKCRGWRMCVSGCPYKKIYYNWSSGKS
HHHHHHCCCHHHHCCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCEEEEECCCCCC
EKCTLCYPRLEAGQPTVCSETCVGRIRYLGVVLYDADRIEAAASTPDEQDLYEEQLKVFL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEEECHHHHHHCCCCCHHHHHHHHHHEEE
DPSDPKVISEARKAGIAEAWLEAARRSPVYKMAMEWKIAFPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCCHH
IQSAASAGKIGLDGEMPDVKSLRIPVKYLANLLTAGKEEPVVTGLERMLAMRAYMRGKTF
HHHHHHCCCCCCCCCCCCHHHEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCH
DGVLDEAIAARVGLSGAQIEEMYRVMAIANYEDRFVIPTSHREMGIEDAYDLRGSCGFSF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHCCCCHHHHCCCCCCCCC
GNGCSGGTTGTNLFGAPKPAKAKTPMEVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTNREGVEYAWFNNVETKPGIGYPKDWENQD
CCCCEEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWKGGWTRRTNGKIEPRIGSKWRILANIFANPDLPEIDDYYEPFDFDYQHLQRAPEVQAF
CCCCCCEECCCCEECCCCCCCEEEEEHHHCCCCCCCCHHCCCCCCCCHHHHHCCCCCCCC
PTARPRSKITGERMEKIEWGPNWEEILGGEFAKRRQDKNFEGVEEEIYGQFENTFMMYLP
CCCCCCHHCCHHHHHHCCCCCCHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGAIYKREEDGVVLIDQDKCRGWRMCVSGCPYKKIYYNWSSGKS
HHHHHHCCCHHHHCCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCEEEEECCCCCC
EKCTLCYPRLEAGQPTVCSETCVGRIRYLGVVLYDADRIEAAASTPDEQDLYEEQLKVFL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEEECHHHHHHCCCCCHHHHHHHHHHEEE
DPSDPKVISEARKAGIAEAWLEAARRSPVYKMAMEWKIAFPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCCHH
IQSAASAGKIGLDGEMPDVKSLRIPVKYLANLLTAGKEEPVVTGLERMLAMRAYMRGKTF
HHHHHHCCCCCCCCCCCCHHHEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCH
DGVLDEAIAARVGLSGAQIEEMYRVMAIANYEDRFVIPTSHREMGIEDAYDLRGSCGFSF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHCCCCHHHHCCCCCCCCC
GNGCSGGTTGTNLFGAPKPAKAKTPMEVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]