Click here to switch to the map view.

The map label for this gene is moaA [H]

Identifier: 170748996

GI number: 170748996

Start: 2741326

End: 2742348

Strand: Reverse

Name: moaA [H]

Synonym: Mrad2831_2586

Alternate gene names: 170748996

Gene position: 2742348-2741326 (Counterclockwise)

Preceding gene: 170748997

Following gene: 170748995

Centisome position: 45.12

GC content: 70.97

Gene sequence:

>1023_bases
ATGACCTGTGCGGCGTTCCCCGGCTCCGGCCAGTCCCTCGTCGACGGCTACGGCCGGCACGTGAGCTACCTGCGCGTCTC
CGTTACCGACCGCTGCGACCTGCGATGCACCTACTGCATGTCGGAGCAGATGACCTTCCTGCCCCGGCGTGACCTCCTGA
CCCTGGAGGAACTCGACCGGCTTTGCTCCGCCTTCGTGGAGCGCGGCGTGCGCCGCATCCGGCTCACCGGCGGCGAGCCC
CTAGTCCGCCGGGACGTCATCCGCTTGCTCGAATCCCTCTCCCGGCACCTCGACACGGGCACGCTCGACGAGCTGACCCT
GACCACCAACGGCACGATGCTGGCGCACCGCGCCCGGGACCTCGCAGCCTGCGGCGTCCGGCGCATTAACGTCTCGCTCG
ATACCCTCGACCCGGACCGCTACCGCGCCGTCACCCGCCGCGGCGACCTCTCGGTGGTGCTCGACGGACTCGACGCCGCG
CAGGCGGCGGGCTTGGCCGTGAAGCTCAATGTTGTCGCCCTGCGGGACGTCAACGAGGACGAGCTTGAGGACCTCGTCCG
GTTCGCCCATGGGCGCGGCATGGACCTCACGCTAATCGAGGTCATGCCGCTCGGCGACACCGGTCACGACCGCGCCACGC
GCTTCCTCCCGCTCGATGAGGTGCGTAGACGCCTGTCGCGAAGCCTCACCCTCGATCCGCTCGCCTACCGTTCGGCGGGC
CCGGCCCGCTACGTGCGCGTGGCCGAGACCGGCGGGCGGCTCGGCTTCATCACGCCGCTGACCCACAACTTCTGCGAGGG
CTGCAATCGGGTGCGGGTGACCTGCGCCGGGACGCTCTACACCTGCCTCGGGCACGAGGAGGGCCGGGACCTGCGCGCTG
CCCTGCGAGGCTCGGAAGCGGACGGGCTGCTGCAGGCGGCCATCGACGACGCCATCGCGCGCAAGCCCAAGGGCCACGAC
TTCGTCGCCGACCGCATCTCCGCTCCCGCCGTCGCGCGGCACATGAGCGTCACCGGGGGATAG

Upstream 100 bases:

>100_bases
CAGGCCACAGGTGGCATCCCCTGCCTGCGGATGCGGCCACTGAACCGTTCACGCCATGGCGGTCGAGGCATCTCGCCAAA
CCGCCTGCGGAGATCCTGTC

Downstream 100 bases:

>100_bases
GACGCCATGGTGGCTCGCATCGGCATCCTCACCGTGTCGGACCGCGCCGCGGCGGGCGTCTACGCGGACGAGGGCGGCCC
TGCCATCCGGGCGGAGCTCA

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTCAAFPGSGQSLVDGYGRHVSYLRVSVTDRCDLRCTYCMSEQMTFLPRRDLLTLEELDRLCSAFVERGVRRIRLTGGEP
LVRRDVIRLLESLSRHLDTGTLDELTLTTNGTMLAHRARDLAACGVRRINVSLDTLDPDRYRAVTRRGDLSVVLDGLDAA
QAAGLAVKLNVVALRDVNEDELEDLVRFAHGRGMDLTLIEVMPLGDTGHDRATRFLPLDEVRRRLSRSLTLDPLAYRSAG
PARYVRVAETGGRLGFITPLTHNFCEGCNRVRVTCAGTLYTCLGHEEGRDLRAALRGSEADGLLQAAIDDAIARKPKGHD
FVADRISAPAVARHMSVTGG

Sequences:

>Translated_340_residues
MTCAAFPGSGQSLVDGYGRHVSYLRVSVTDRCDLRCTYCMSEQMTFLPRRDLLTLEELDRLCSAFVERGVRRIRLTGGEP
LVRRDVIRLLESLSRHLDTGTLDELTLTTNGTMLAHRARDLAACGVRRINVSLDTLDPDRYRAVTRRGDLSVVLDGLDAA
QAAGLAVKLNVVALRDVNEDELEDLVRFAHGRGMDLTLIEVMPLGDTGHDRATRFLPLDEVRRRLSRSLTLDPLAYRSAG
PARYVRVAETGGRLGFITPLTHNFCEGCNRVRVTCAGTLYTCLGHEEGRDLRAALRGSEADGLLQAAIDDAIARKPKGHD
FVADRISAPAVARHMSVTGG
>Mature_339_residues
TCAAFPGSGQSLVDGYGRHVSYLRVSVTDRCDLRCTYCMSEQMTFLPRRDLLTLEELDRLCSAFVERGVRRIRLTGGEPL
VRRDVIRLLESLSRHLDTGTLDELTLTTNGTMLAHRARDLAACGVRRINVSLDTLDPDRYRAVTRRGDLSVVLDGLDAAQ
AAGLAVKLNVVALRDVNEDELEDLVRFAHGRGMDLTLIEVMPLGDTGHDRATRFLPLDEVRRRLSRSLTLDPLAYRSAGP
ARYVRVAETGGRLGFITPLTHNFCEGCNRVRVTCAGTLYTCLGHEEGRDLRAALRGSEADGLLQAAIDDAIARKPKGHDF
VADRISAPAVARHMSVTGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI22027536, Length=312, Percent_Identity=36.5384615384615, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI116734668, Length=312, Percent_Identity=36.5384615384615, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI1786999, Length=332, Percent_Identity=32.5301204819277, Blast_Score=178, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17561210, Length=311, Percent_Identity=33.1189710610932, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI32566331, Length=341, Percent_Identity=33.1378299120235, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI32566329, Length=319, Percent_Identity=33.2288401253918, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28574490, Length=318, Percent_Identity=38.9937106918239, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI28574488, Length=311, Percent_Identity=39.2282958199357, Blast_Score=202, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 37384; Mature: 37253

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCAAFPGSGQSLVDGYGRHVSYLRVSVTDRCDLRCTYCMSEQMTFLPRRDLLTLEELDR
CCCCCCCCCCHHHHHHHCCEEEEEEEEECCCCCCEEEEECCCCHHHCCCCCCCCHHHHHH
LCSAFVERGVRRIRLTGGEPLVRRDVIRLLESLSRHLDTGTLDELTLTTNGTMLAHRARD
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEHHHHHH
LAACGVRRINVSLDTLDPDRYRAVTRRGDLSVVLDGLDAAQAAGLAVKLNVVALRDVNED
HHHCCEEEEEEEEECCCCHHHHHHHCCCCEEEEEECCCHHHHCCEEEEEEEEEEECCCHH
ELEDLVRFAHGRGMDLTLIEVMPLGDTGHDRATRFLPLDEVRRRLSRSLTLDPLAYRSAG
HHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHEECCCHHHHHHHHHHCCCCCCHHHCCCC
PARYVRVAETGGRLGFITPLTHNFCEGCNRVRVTCAGTLYTCLGHEEGRDLRAALRGSEA
CHHEEEEECCCCCEEEECCHHHHHHCCCCEEEEEECHHHHHHHCCCCCCHHHHHHCCCCC
DGLLQAAIDDAIARKPKGHDFVADRISAPAVARHMSVTGG
CHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHEECCCC
>Mature Secondary Structure 
TCAAFPGSGQSLVDGYGRHVSYLRVSVTDRCDLRCTYCMSEQMTFLPRRDLLTLEELDR
CCCCCCCCCHHHHHHHCCEEEEEEEEECCCCCCEEEEECCCCHHHCCCCCCCCHHHHHH
LCSAFVERGVRRIRLTGGEPLVRRDVIRLLESLSRHLDTGTLDELTLTTNGTMLAHRARD
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEHHHHHH
LAACGVRRINVSLDTLDPDRYRAVTRRGDLSVVLDGLDAAQAAGLAVKLNVVALRDVNED
HHHCCEEEEEEEEECCCCHHHHHHHCCCCEEEEEECCCHHHHCCEEEEEEEEEEECCCHH
ELEDLVRFAHGRGMDLTLIEVMPLGDTGHDRATRFLPLDEVRRRLSRSLTLDPLAYRSAG
HHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHEECCCHHHHHHHHHHCCCCCCHHHCCCC
PARYVRVAETGGRLGFITPLTHNFCEGCNRVRVTCAGTLYTCLGHEEGRDLRAALRGSEA
CHHEEEEECCCCCEEEECCHHHHHHCCCCEEEEEECHHHHHHHCCCCCCHHHHHHCCCCC
DGLLQAAIDDAIARKPKGHDFVADRISAPAVARHMSVTGG
CHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]