Click here to switch to the map view.

The map label for this gene is insF1 [H]

Identifier: 170748982

GI number: 170748982

Start: 2729050

End: 2729910

Strand: Reverse

Name: insF1 [H]

Synonym: Mrad2831_2570

Alternate gene names: 170748982

Gene position: 2729910-2729050 (Counterclockwise)

Preceding gene: 170748983

Following gene: 170748976

Centisome position: 44.92

GC content: 67.48

Gene sequence:

>861_bases
GTGACCTTCGCCTTCATCGAGCAGCATGCGCGGACCTGGCCGGTGCGTCTCATGTGCCGCGTGCTCGGAGTCTCACCCAG
CGGCTTCTACGGATGGCGGTCACGGCCTGAGAGCGCCCGCTCGGCCTCCAATCGCCAACTCCTGGACGACGTCCGGCGCA
TCCATGCCGCCCACCACCGACGCTATGGTGCCCCACGCGTGCATGCCGCTCTGCGAGCCGAGGGCCGGTCCGTCAGCCGC
GGGCGGGTGGAGCGTCTCATGCGCCGGCATGGCATTCGTGCGCTGGCGGGGCGTCGGTTCCGGCCCTGTACGACCGACAG
CCGTCACGATCTCCCCATCGCGCCCAATCTCCTCAAGCAGGACTTCTCAGCCGCGCGACCCGACACGGTCTGGCTGGCCG
ATATCACCTACCTGCCGACCGGCGAGGGCTGGCTGTACTTGGCCGCTGTCCTCGATCTGGCCACGCGCAAGATCGTCGGT
TGGTCGATGCGCGATCACATGCGCGCCGAGCTGAGCGTCGCGGCATTGATGATGGCCGCCCAGCGGCAGCGGCCGGCCGC
CGGGCTCATCTGCCACTCGGATCGCGGCAGCCAGTACGCGGCCGAGGCCTATCGGAAGCAGCTCGCCGCAATGGGGGCCA
GGCCGTCCATGAGTCGGAGCGCCTGTTGCTACGATAACGCCCCGATGGAGAGCTTCTTCCACACGCTCAAGGTCGAGCTC
GTCCATCAGCGACGATGGGCGACCCGGGATGAAGCGCGGCGCGATCTGTTCGCCTACATCGAGGGCTACTACAATCGGCA
ACGCATCCACTCGGCACTCGGCTATCTCACGCCCGAACAAGCCGAGAGGACCGCGAAATGA

Upstream 100 bases:

>100_bases
AGGCCGCCGAGATCGCCCGACTGCGGCGGGAGCTCGACCGCACGCGTATGGAGCGCGACGTACTAAAAAAAGCTATCGGC
ATCTTCGCGGAGATGCCCAA

Downstream 100 bases:

>100_bases
TCTCCCTGTGTCCGCGACACCGAGGGAGGATCACTTCGTCGAATGACGCTACGGCGCGTCCGTGCGCGTCGGGCAACCGG
GACCGTCAGGGACGGGCCCG

Product: integrase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTFAFIEQHARTWPVRLMCRVLGVSPSGFYGWRSRPESARSASNRQLLDDVRRIHAAHHRRYGAPRVHAALRAEGRSVSR
GRVERLMRRHGIRALAGRRFRPCTTDSRHDLPIAPNLLKQDFSAARPDTVWLADITYLPTGEGWLYLAAVLDLATRKIVG
WSMRDHMRAELSVAALMMAAQRQRPAAGLICHSDRGSQYAAEAYRKQLAAMGARPSMSRSACCYDNAPMESFFHTLKVEL
VHQRRWATRDEARRDLFAYIEGYYNRQRIHSALGYLTPEQAERTAK

Sequences:

>Translated_286_residues
MTFAFIEQHARTWPVRLMCRVLGVSPSGFYGWRSRPESARSASNRQLLDDVRRIHAAHHRRYGAPRVHAALRAEGRSVSR
GRVERLMRRHGIRALAGRRFRPCTTDSRHDLPIAPNLLKQDFSAARPDTVWLADITYLPTGEGWLYLAAVLDLATRKIVG
WSMRDHMRAELSVAALMMAAQRQRPAAGLICHSDRGSQYAAEAYRKQLAAMGARPSMSRSACCYDNAPMESFFHTLKVEL
VHQRRWATRDEARRDLFAYIEGYYNRQRIHSALGYLTPEQAERTAK
>Mature_285_residues
TFAFIEQHARTWPVRLMCRVLGVSPSGFYGWRSRPESARSASNRQLLDDVRRIHAAHHRRYGAPRVHAALRAEGRSVSRG
RVERLMRRHGIRALAGRRFRPCTTDSRHDLPIAPNLLKQDFSAARPDTVWLADITYLPTGEGWLYLAAVLDLATRKIVGW
SMRDHMRAELSVAALMMAAQRQRPAAGLICHSDRGSQYAAEAYRKQLAAMGARPSMSRSACCYDNAPMESFFHTLKVELV
HQRRWATRDEARRDLFAYIEGYYNRQRIHSALGYLTPEQAERTAK

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=243, Percent_Identity=32.5102880658436, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI1788405, Length=286, Percent_Identity=46.1538461538462, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1787263, Length=286, Percent_Identity=46.1538461538462, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1786753, Length=286, Percent_Identity=46.1538461538462, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1786570, Length=286, Percent_Identity=46.1538461538462, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1786490, Length=286, Percent_Identity=46.1538461538462, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1789981, Length=270, Percent_Identity=30.7407407407407, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32717; Mature: 32586

Theoretical pI: Translated: 11.11; Mature: 11.11

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFAFIEQHARTWPVRLMCRVLGVSPSGFYGWRSRPESARSASNRQLLDDVRRIHAAHHR
CCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RYGAPRVHAALRAEGRSVSRGRVERLMRRHGIRALAGRRFRPCTTDSRHDLPIAPNLLKQ
HCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
DFSAARPDTVWLADITYLPTGEGWLYLAAVLDLATRKIVGWSMRDHMRAELSVAALMMAA
HHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QRQRPAAGLICHSDRGSQYAAEAYRKQLAAMGARPSMSRSACCYDNAPMESFFHTLKVEL
HHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VHQRRWATRDEARRDLFAYIEGYYNRQRIHSALGYLTPEQAERTAK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
TFAFIEQHARTWPVRLMCRVLGVSPSGFYGWRSRPESARSASNRQLLDDVRRIHAAHHR
CHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RYGAPRVHAALRAEGRSVSRGRVERLMRRHGIRALAGRRFRPCTTDSRHDLPIAPNLLKQ
HCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
DFSAARPDTVWLADITYLPTGEGWLYLAAVLDLATRKIVGWSMRDHMRAELSVAALMMAA
HHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QRQRPAAGLICHSDRGSQYAAEAYRKQLAAMGARPSMSRSACCYDNAPMESFFHTLKVEL
HHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VHQRRWATRDEARRDLFAYIEGYYNRQRIHSALGYLTPEQAERTAK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]