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The map label for this gene is merA [H]

Identifier: 170748962

GI number: 170748962

Start: 2710697

End: 2712115

Strand: Reverse

Name: merA [H]

Synonym: Mrad2831_2549

Alternate gene names: 170748962

Gene position: 2712115-2710697 (Counterclockwise)

Preceding gene: 170748965

Following gene: 170748961

Centisome position: 44.62

GC content: 70.33

Gene sequence:

>1419_bases
ATGTCTTGGACGCACGACGTGGTGGTTGTCGGTGCGGGGGCGGCCGGGCTCACCGCCGCGGGAGGCTTGGCGCAGCTTGG
CCTGCGGGTGGCCTTGATCGAGGCGGGGGCGATGGGGGGCGAATGCCTCAATACGGGCTGCGTTCCGTCCAAGGCCCTTC
TCGCAATCGCTGCCCGGGCGCAGGCGGTCCGCGACATGGGACGCTTCGGTATCCGCGCGCAGGAGCCGCAAGTCGATTTT
TCGGGCGTGATGGCGCAGGTGCGCGGCGTCATCGCCGAGATCGCCCCGCATGACGCGCAGGAGCGTTTCGAAGCCTGGGG
CGCCGAGGTGATCCGGGCACATGCCCGCTTCACGGCCCCCCGGACGCTGCTGGTCGCCGGCCGAACCCTCAGGGCACCGA
GAATCGTGCTTGCGGTGGGGTCGCGCCCTGCCGTGCCGTCCATCCCGGGTCTCGATGCCGTCCCCTTTTTGACGAACGAG
ACGCTGTTTTCCATCGATGCCTTTCCGGAACGCCTCGTCGTGCTGGGCGCCGGTTCGGTCGGCATGGAGATGGCGCAGGC
ATTCCGTCGACTCGGGGCCGAAGTTGCGGTGATCGACCGGGGCCCCCCCCTATCCCGGGACGAGCCTGAAGCCGCGGCGC
TGGTGTCGGAGCGACTGGAGGCGGAAGGCATCGCGTTCCACCGGGCCGCCATCCGAGGCGTGTCGTCCGTCGGAGGCGGC
GTCGCGGTCGCTTTGGATGACGGCGACACCGTCGGGGGAAGCCATCTCCTGGTGGCGCTGGGGCGATCCGCGAACCTTCA
TGGGCTTGGCCTCGAAGAGGCCGGCGTGGGTACGGACGGCGGAGGCATCGTCGTCGACGACCGATTGCGGACCAGCGCAC
GCGGCGTCTACGCCATCGGCGATTGCCGCGACGGACCTCGCCTGACCCACGCGGCCGGCTACGAGGGCGCGCGGATCGTC
GCCGCCATCGGGTTCGGGCTGCGTGCCCGGGTCGACTACCGCGCCCTGCCGCGGGTCGCCTACACCAGCCCCGAACTCGC
GCAGGTCGGGATGACGGAAGCGGAGGCAAGGGAGGGGGGCGGCCGTGTCACGGCGATGCGCGAACCCTTCGCCGACAACG
ACCGGGCCGTCGCCGAGGGAGCTTCCGTCGGCTTTCTCAAGGTCGTACGACGGAATGGCCGGATCGTCGGTGCCTGCCTC
GTGGGAGAGCGGGTGGGCGACCTGACGATGCCGTGGGTTCTCTCCATCGGCGGTGCCAAACCGACACCCTGGGCGTTGTC
GGGCATGATCCTGCCGTATCCGACCCGTTCCGAGATCACGAAGGCAGCCGCGTTCGCCATGTACGCACCGCGCCTGTTCT
CGCGACCGGCGCGCATATGGGCCGGGGTCCTCGCAGCGCTTCGGCGCCGGCTGGGCTGA

Upstream 100 bases:

>100_bases
GGTGTTTTCGAGGCTCATGCGGATCGCTCCTGTCGGTCGGGGGAGACCACGCCGCGCGGAGCGGGGTCAATCGCGCGGGC
TGACGCAAGGAAGGATCGTG

Downstream 100 bases:

>100_bases
GGCGGTCCCGATGGCTTCCGCTCCTCCTCCTGCCGGCCCCTCATGCTCGCCGTTCCGTGACAGTCGGGCTCCGAGGCTCG
CGCCAGGCAAGTTCCGGGAT

Product: pyridine nucleotide-disulfide oxidoreductase dimerisation region

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSWTHDVVVVGAGAAGLTAAGGLAQLGLRVALIEAGAMGGECLNTGCVPSKALLAIAARAQAVRDMGRFGIRAQEPQVDF
SGVMAQVRGVIAEIAPHDAQERFEAWGAEVIRAHARFTAPRTLLVAGRTLRAPRIVLAVGSRPAVPSIPGLDAVPFLTNE
TLFSIDAFPERLVVLGAGSVGMEMAQAFRRLGAEVAVIDRGPPLSRDEPEAAALVSERLEAEGIAFHRAAIRGVSSVGGG
VAVALDDGDTVGGSHLLVALGRSANLHGLGLEEAGVGTDGGGIVVDDRLRTSARGVYAIGDCRDGPRLTHAAGYEGARIV
AAIGFGLRARVDYRALPRVAYTSPELAQVGMTEAEAREGGGRVTAMREPFADNDRAVAEGASVGFLKVVRRNGRIVGACL
VGERVGDLTMPWVLSIGGAKPTPWALSGMILPYPTRSEITKAAAFAMYAPRLFSRPARIWAGVLAALRRRLG

Sequences:

>Translated_472_residues
MSWTHDVVVVGAGAAGLTAAGGLAQLGLRVALIEAGAMGGECLNTGCVPSKALLAIAARAQAVRDMGRFGIRAQEPQVDF
SGVMAQVRGVIAEIAPHDAQERFEAWGAEVIRAHARFTAPRTLLVAGRTLRAPRIVLAVGSRPAVPSIPGLDAVPFLTNE
TLFSIDAFPERLVVLGAGSVGMEMAQAFRRLGAEVAVIDRGPPLSRDEPEAAALVSERLEAEGIAFHRAAIRGVSSVGGG
VAVALDDGDTVGGSHLLVALGRSANLHGLGLEEAGVGTDGGGIVVDDRLRTSARGVYAIGDCRDGPRLTHAAGYEGARIV
AAIGFGLRARVDYRALPRVAYTSPELAQVGMTEAEAREGGGRVTAMREPFADNDRAVAEGASVGFLKVVRRNGRIVGACL
VGERVGDLTMPWVLSIGGAKPTPWALSGMILPYPTRSEITKAAAFAMYAPRLFSRPARIWAGVLAALRRRLG
>Mature_471_residues
SWTHDVVVVGAGAAGLTAAGGLAQLGLRVALIEAGAMGGECLNTGCVPSKALLAIAARAQAVRDMGRFGIRAQEPQVDFS
GVMAQVRGVIAEIAPHDAQERFEAWGAEVIRAHARFTAPRTLLVAGRTLRAPRIVLAVGSRPAVPSIPGLDAVPFLTNET
LFSIDAFPERLVVLGAGSVGMEMAQAFRRLGAEVAVIDRGPPLSRDEPEAAALVSERLEAEGIAFHRAAIRGVSSVGGGV
AVALDDGDTVGGSHLLVALGRSANLHGLGLEEAGVGTDGGGIVVDDRLRTSARGVYAIGDCRDGPRLTHAAGYEGARIVA
AIGFGLRARVDYRALPRVAYTSPELAQVGMTEAEAREGGGRVTAMREPFADNDRAVAEGASVGFLKVVRRNGRIVGACLV
GERVGDLTMPWVLSIGGAKPTPWALSGMILPYPTRSEITKAAAFAMYAPRLFSRPARIWAGVLAALRRRLG

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=435, Percent_Identity=29.6551724137931, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=27.645788336933, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI33519430, Length=412, Percent_Identity=25.7281553398058, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI33519428, Length=412, Percent_Identity=25.7281553398058, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI33519426, Length=412, Percent_Identity=25.7281553398058, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI148277065, Length=412, Percent_Identity=25.7281553398058, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI148277071, Length=412, Percent_Identity=25.7281553398058, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI22035672, Length=443, Percent_Identity=27.765237020316, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI291045266, Length=350, Percent_Identity=26.2857142857143, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI291045268, Length=327, Percent_Identity=25.9938837920489, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1786307, Length=418, Percent_Identity=31.1004784688995, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789915, Length=358, Percent_Identity=32.4022346368715, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87082354, Length=465, Percent_Identity=28.6021505376344, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI87081717, Length=446, Percent_Identity=28.9237668161435, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32565766, Length=471, Percent_Identity=29.723991507431, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17557007, Length=415, Percent_Identity=27.9518072289157, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=361, Percent_Identity=29.6398891966759, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=361, Percent_Identity=29.6398891966759, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=456, Percent_Identity=23.6842105263158, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=29.559748427673, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6325166, Length=429, Percent_Identity=25.6410256410256, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325240, Length=476, Percent_Identity=26.4705882352941, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21358499, Length=438, Percent_Identity=30.365296803653, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24640553, Length=387, Percent_Identity=30.4909560723514, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24640549, Length=387, Percent_Identity=30.4909560723514, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640551, Length=387, Percent_Identity=30.4909560723514, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17737741, Length=387, Percent_Identity=28.1653746770026, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 49117; Mature: 48986

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWTHDVVVVGAGAAGLTAAGGLAQLGLRVALIEAGAMGGECLNTGCVPSKALLAIAARA
CCCCCCEEEEECCCCCHHHCCCHHHHCEEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHH
QAVRDMGRFGIRAQEPQVDFSGVMAQVRGVIAEIAPHDAQERFEAWGAEVIRAHARFTAP
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
RTLLVAGRTLRAPRIVLAVGSRPAVPSIPGLDAVPFLTNETLFSIDAFPERLVVLGAGSV
CEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCEECCCCEEEECCCCCEEEEEECCHH
GMEMAQAFRRLGAEVAVIDRGPPLSRDEPEAAALVSERLEAEGIAFHRAAIRGVSSVGGG
HHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
VAVALDDGDTVGGSHLLVALGRSANLHGLGLEEAGVGTDGGGIVVDDRLRTSARGVYAIG
EEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHCCCCCCCCCEEECCCCCCCCCCEEEEE
DCRDGPRLTHAAGYEGARIVAAIGFGLRARVDYRALPRVAYTSPELAQVGMTEAEAREGG
CCCCCCCCEECCCCCCCEEEEEECCCCEEEECHHHCCCEECCCCHHHHCCCCHHHHHCCC
GRVTAMREPFADNDRAVAEGASVGFLKVVRRNGRIVGACLVGERVGDLTMPWVLSIGGAK
CEEEEECCCCCCCCCHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHEEECCCC
PTPWALSGMILPYPTRSEITKAAAFAMYAPRLFSRPARIWAGVLAALRRRLG
CCCHHHCCEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SWTHDVVVVGAGAAGLTAAGGLAQLGLRVALIEAGAMGGECLNTGCVPSKALLAIAARA
CCCCCEEEEECCCCCHHHCCCHHHHCEEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHH
QAVRDMGRFGIRAQEPQVDFSGVMAQVRGVIAEIAPHDAQERFEAWGAEVIRAHARFTAP
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
RTLLVAGRTLRAPRIVLAVGSRPAVPSIPGLDAVPFLTNETLFSIDAFPERLVVLGAGSV
CEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCEECCCCEEEECCCCCEEEEEECCHH
GMEMAQAFRRLGAEVAVIDRGPPLSRDEPEAAALVSERLEAEGIAFHRAAIRGVSSVGGG
HHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
VAVALDDGDTVGGSHLLVALGRSANLHGLGLEEAGVGTDGGGIVVDDRLRTSARGVYAIG
EEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHCCCCCCCCCEEECCCCCCCCCCEEEEE
DCRDGPRLTHAAGYEGARIVAAIGFGLRARVDYRALPRVAYTSPELAQVGMTEAEAREGG
CCCCCCCCEECCCCCCCEEEEEECCCCEEEECHHHCCCEECCCCHHHHCCCCHHHHHCCC
GRVTAMREPFADNDRAVAEGASVGFLKVVRRNGRIVGACLVGERVGDLTMPWVLSIGGAK
CEEEEECCCCCCCCCHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHEEECCCC
PTPWALSGMILPYPTRSEITKAAAFAMYAPRLFSRPARIWAGVLAALRRRLG
CCCHHHCCEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2536669; 10559175; 2067577 [H]