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The map label for this gene is opuAB [H]

Identifier: 170748909

GI number: 170748909

Start: 2658642

End: 2659493

Strand: Reverse

Name: opuAB [H]

Synonym: Mrad2831_2491

Alternate gene names: 170748909

Gene position: 2659493-2658642 (Counterclockwise)

Preceding gene: 170748910

Following gene: 170748908

Centisome position: 43.76

GC content: 68.9

Gene sequence:

>852_bases
ATGGACTGGAACGTCCCGAAATTTCCCCTCGACACGCTCAGCGACGACGGGCTCGATTGGCTCACCGACCATGGCGCTTG
GCTGACCCGCGCCGTCAGCCGCTCGGTCTCGAACGGGATCGACACGCTGACCGCGGCCCTCATCTCGGTGCCGCCCTGGG
CGGTCATCGCGGTGGCGGCCCTGGCGACCTACAAGGTCGGGGGGCGCAAGGTCGGGCTCCTGACGCTCGCCGGCCTGCTG
TTCCTGTGGGATCTCCGGCTCTGGCAGTCCACGGTCGAGACCCTGGTCCTCGTGAGCCTCGCGACCGTGATGGCCCTCCT
GATCGGGATCCCGGTGGGCATCTGGTTCGCCCTCAGCCGGTGGGCCTGGAAGGTGTTCTCGCCGGTGCTGGACATGATGC
AGACGCTGCCGAGCTTCGTCTATCTCATCCCGGCGCTGCCCTTCTTCGGCCTCGGTGCGGTCTCGGCCTGCTTCGCGACC
ATCATCTTCTCCGTACCGCCCGTGATCCGTCTCACGACGCTCGGCATCCGCAACGTGCCGACGGAACTGATCGAGGCGTC
CGACGCCTTCGGCGGTTCGGCGACGCAGAAGCTCCTCAAGGTGCAGTTGCCGCTCGCGCTGCCGACCATCATGGCCGGCG
TCAACCAGACGATGATGCTCGCCCTGTCCATGGTCGTCATCGCCGCGATGATCGGGGCCGGAGGCCTCGGGCGCGAGGTG
TGGCAGTCGATCCAGCGCCTCGAGGCGGGCGCCGGCTTCGAGGCCGGGCTCGGCATCGTGATCCTCGCCGTCATCCTCGA
CCGCTTCACCCAGGCCCTCGCCGCGCGCGCCCACCCGCACGGCTCGGCCTGA

Upstream 100 bases:

>100_bases
CGAGCCGGGACATCGTCCGCGCCCTGGCGGCGCGCCCGGGCACGCACGGTCGGCCGACCCTCAGCGCCGCCCCCCTTCAA
AAGCCCACAGGAGCGGAAGG

Downstream 100 bases:

>100_bases
CGGACGCCGCGCCGCCGCCGAAGCCGCCGCCGCCCCGGCTGCTCGCGGCGCCCGGTACCGCCGGGCGCCACCCTGTCGCG
CGCCGATTCTCGAAGCTCCG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MDWNVPKFPLDTLSDDGLDWLTDHGAWLTRAVSRSVSNGIDTLTAALISVPPWAVIAVAALATYKVGGRKVGLLTLAGLL
FLWDLRLWQSTVETLVLVSLATVMALLIGIPVGIWFALSRWAWKVFSPVLDMMQTLPSFVYLIPALPFFGLGAVSACFAT
IIFSVPPVIRLTTLGIRNVPTELIEASDAFGGSATQKLLKVQLPLALPTIMAGVNQTMMLALSMVVIAAMIGAGGLGREV
WQSIQRLEAGAGFEAGLGIVILAVILDRFTQALAARAHPHGSA

Sequences:

>Translated_283_residues
MDWNVPKFPLDTLSDDGLDWLTDHGAWLTRAVSRSVSNGIDTLTAALISVPPWAVIAVAALATYKVGGRKVGLLTLAGLL
FLWDLRLWQSTVETLVLVSLATVMALLIGIPVGIWFALSRWAWKVFSPVLDMMQTLPSFVYLIPALPFFGLGAVSACFAT
IIFSVPPVIRLTTLGIRNVPTELIEASDAFGGSATQKLLKVQLPLALPTIMAGVNQTMMLALSMVVIAAMIGAGGLGREV
WQSIQRLEAGAGFEAGLGIVILAVILDRFTQALAARAHPHGSA
>Mature_283_residues
MDWNVPKFPLDTLSDDGLDWLTDHGAWLTRAVSRSVSNGIDTLTAALISVPPWAVIAVAALATYKVGGRKVGLLTLAGLL
FLWDLRLWQSTVETLVLVSLATVMALLIGIPVGIWFALSRWAWKVFSPVLDMMQTLPSFVYLIPALPFFGLGAVSACFAT
IIFSVPPVIRLTTLGIRNVPTELIEASDAFGGSATQKLLKVQLPLALPTIMAGVNQTMMLALSMVVIAAMIGAGGLGREV
WQSIQRLEAGAGFEAGLGIVILAVILDRFTQALAARAHPHGSA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=274, Percent_Identity=47.4452554744526, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1788451, Length=199, Percent_Identity=31.1557788944724, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788449, Length=190, Percent_Identity=28.9473684210526, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786564, Length=166, Percent_Identity=33.7349397590361, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30156; Mature: 30156

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]